Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,968,508 Δ1 bp coding (594/1104 nt) wecA → UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,968,5080A.95.5% 177.1 / 1.0 45coding (594/1104 nt)wecAUDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base . (17/25);  total (19/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAGGGCAAAACCCATCCCATCAGCC  >  NC_000913/3968374‑3968644
                                                                                                                                      |                                                                                                                                        
aTGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGGCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATg                                                                                                                                 <  2:623718/143‑1 (MQ=255)
 tggtggATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGTAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGc                                                                                                                                <  2:62939/143‑1 (MQ=255)
 tGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGc                                                                                                                                >  2:721499/1‑143 (MQ=255)
   gTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCtt                                                                                                                              >  2:311588/1‑143 (MQ=255)
    ttGATGGCAGTGATGGCTTGTTGGGTGGGTTGTCCTGCGACTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTa                                                                                                                             <  2:690394/143‑1 (MQ=255)
         gtcATTGATGGCTTGCTGGGGGGGTTGTCCTGCGTCGAGGTAGCAGCAATCGGTATGATTGTGTGGTCCAACTTGCAAACCGGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCtt                                                                                                                        <  2:234966/141‑1 (MQ=255)
         ggCAGTGATGGCGTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCtt                                                                                                                        <  1:48761/143‑1 (MQ=255)
            aTTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAACAGGGACGATTTTGTGGTTCGGCGGGCAAACCAGCCTCGCAAGCTGGTGCTTTGCGCAGCTCGCCGCCGTCATG‑CACCCATCATCGTTAACCGTggg                                                                                                                     >  1:848755/1‑142 (MQ=255)
                  ggCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCCGCAATCGGTCTGCTTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCGTTGCGATGATCGCCGCCATCCT‑CCCTACATCACGCGTGACCTCGGTACCCTg                                                                                                               >  2:327155/1‑143 (MQ=255)
                   gCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTgg                                                                                                              <  1:626465/143‑1 (MQ=255)
                          ggggggGTTGTCGGGCGCCTCGTCTGGAGCAATGGGTATGATTTTGTGGGTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTcgccg                                                                                                       <  1:609794/143‑1 (MQ=255)
                              gggTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCACTCTTAACCTTCGTCTCCTGGGTCGCCGCCAc                                                                                                   >  2:411280/1‑143 (MQ=255)
                                 ttGTCCTGCGTCTAGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACaaa                                                                                                <  2:161755/143‑1 (MQ=255)
                                 ttGTCCTGCGTCGCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACaaa                                                                                                <  2:46667/143‑1 (MQ=255)
                                 ttGTCCGGCGTCTAGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCGTGGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACaaa                                                                                                <  2:166509/143‑1 (MQ=255)
                                     ccTGCGGCTCGTTTGCCGCCAGCCGGATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCt                                                                                            <  1:397597/143‑1 (MQ=255)
                                        gCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTa                                                                                         >  1:963944/1‑143 (MQ=255)
                                         cGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGAGGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGCATCCTGGGTCGCCGCTACAAACGCTTTAt                                                                                        >  2:328448/1‑143 (MQ=255)
                                          gTCTCGTTTGCACCAATCGGGATGATTTTGGCGTGCGTTGGGCAAACCGTCCTCGCGATCTGGTGCTTAGAGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTAGCCTGGGTCGCCGCTACAAAGTCTTTATg                                                                                       <  1:247862/143‑1 (MQ=255)
                                                    cagcaATCGGTATGACTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCgg                                                                             <  2:765814/143‑1 (MQ=255)
                                                     agcaATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGAGATGCggg                                                                            >  2:831314/1‑143 (MQ=255)
                                                     agcaATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCGCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCggg                                                                            <  2:161082/143‑1 (MQ=255)
                                                         aTCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTTACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGt                                                                        <  2:828816/143‑1 (MQ=255)
                                                                tGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAACGTCTTTATGGGTGATGCGGGCAGTACGCTgc                                                                 >  2:538532/1‑142 (MQ=255)
                                                                tGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGa                                                                 >  2:730695/1‑143 (MQ=255)
                                                                           ttCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTAcc                                                      <  2:848932/143‑1 (MQ=255)
                                                                               aCGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGAGGGCAGTACGCTGACTGGGGTTACCGttc                                                  >  1:699563/1‑142 (MQ=255)
                                                                                cGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTTGGTTGCCGCTACAAAGTCTTTATGTGGGATGCGGGTATTACGCTGATTGGTTTTACCGTTAt                                                 <  2:555017/143‑1 (MQ=255)
                                                                                   gCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTg                                              >  2:183499/1‑143 (MQ=255)
                                                                                      aaCCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGAt                                           <  2:514794/143‑1 (MQ=255)
                                                                                             gtCGCTATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTc                                    <  1:171075/142‑1 (MQ=255)
                                                                                             cTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTc                                    <  1:183499/143‑1 (MQ=255)
                                                                                              tCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGCTCCTGCTCg                                   >  2:302105/1‑143 (MQ=255)
                                                                                                          gCTTTGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAgg                       <  2:568537/143‑1 (MQ=255)
                                                                                                           cTTTGCGATGATCGCCGCCATCCTGCC‑GACACGAGGCATAAGCGTGGGAACGTGGGTCGCCGCTACGAAGTGTTTATGGGGGAGGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAggg                      <  2:228858/143‑1 (MQ=255)
                                                                                                            tttGCGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTCCCGTTATCTGGCTCCTTCTCGTAATGAccccgtgc                     >  2:41321/1‑138 (MQ=255)
                                                                                                                cgctgatCGCGGCCGTCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTGTACCGTTATCTGGATCCTGCTCGAAACGACCCAGGGCaaaa                 <  1:387908/140‑1 (MQ=255)
                                                                                                                cGATGATCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGGTGCGGGCCGTACGCTGATTGGTTTTCCCGTTATCTGGATCCTGCCCGAAACGACCCAGCGCaaaa                 >  2:564100/1‑143 (MQ=255)
                                                                                                                cGATGATCGCCGCCATCCTG‑CAGACTTCATGCTGAACCGGTGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAGGGCaaaa                 <  2:375019/143‑1 (MQ=255)
                                                                                                                  atgatCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTCATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGTTCCTGCTCGAAACGACCCAGAGCCAAAcc               >  1:785890/1‑143 (MQ=255)
                                                                                                                  atgatCGCCGCCATCCTGCC‑TACATCATGCTTAACATTCATCGACTACCAAGCGCCTACTAAGTCTGTATGGGTGATGCGGGCAGTACGCCGACGGGTAGCAGCGTTATCTGGATCCTGCTCGAAACGACACAGGGCAAAAcc               >  1:662708/1‑143 (MQ=255)
                                                                                                                      tCGCCGCCATCCTGCC‑TACATCATGCTTAACCTTGGGATCCTGGGTCCCCGCTACAAAGTCTTTATGTGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTCCTCGAAACGACCCAGGGCAAAAcccatc           <  2:662708/143‑1 (MQ=255)
                                                                                                                       cgccgcCATCCTGCC‑TACCTCATGCTTAACCTTGGCATCCTGTGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAGGGCAAAAcccatcc          <  2:53399/143‑1 (MQ=255)
                                                                                                                            ccATCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTGTTACCGTTATCTGGATCATGCTCGAAGCGACCCAGCGCCAAACCCATCCCATCa     >  1:909629/1‑143 (MQ=255)
                                                                                                                               tCCTGCC‑TACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATTCGGGCAGTACGCTGCTTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAGGGCCCGACCACTCCCATCAGca  >  1:816139/1‑142 (MQ=255)
                                                                                                                                      |                                                                                                                                        
ATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAGGGCAAAACCCATCCCATCAGCC  >  NC_000913/3968374‑3968644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑