Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,365,454 | 0 | A | C | 21.9% | 109.7 / 24.9 | 64 | intergenic (‑97/+19) | cutA/dcuA | divalent‑cation tolerance protein, copper sensitivity/C4‑dicarboxylate antiporter |
Reads supporting (aligned to +/- strand): ref base A (21/29); new base C (8/6); total (29/35) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.72e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
CACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCC > NC_000913/4365316‑4365587 | caccacGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGc > 1:560737/1‑143 (MQ=255) ccgcGACAGACGCGGTATTCGAACTTTGTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGccc < 1:997930/140‑1 (MQ=255) acGACAGGCTCGGTCCTCGAACTTTTTTCTCCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTAGACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTTCACGCCCcg < 2:322060/143‑1 (MQ=255) acGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTTCACGcaatg < 2:745751/143‑5 (MQ=255) cGACAGACGCGGTATTCGAACTTTTTTCATAAAGCATAATCACATCGCAATGATTCTTTCGTCCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTTCACGCCCcgc < 2:1004821/143‑1 (MQ=255) aCAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTTCACGCCCcgctt < 1:12007/143‑3 (MQ=255) cAGACGCGGTATTCGAACTTTTTGCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGAt < 1:803450/143‑1 (MQ=255) cgcgGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGAt < 2:769181/143‑1 (MQ=255) gTATTCGAACTTTTTTCATCAGGCATAAACACCTCGCAAGGACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCAtt < 1:320060/143‑1 (MQ=255) tATTCGAACTTTTTTCATCAAGCACCAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGCACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTa < 2:32955/143‑1 (MQ=255) ttcgatcatattgaatcaAGCAAAAACACCTAGCAAGAACAGATGGAACGGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACa < 2:511787/129‑1 (MQ=255) tCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGCAAGAAAGCGGGGCGTGCACGCCCCGCGATTGATCATTAccg > 2:24087/1‑141 (MQ=255) gtttCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGc < 2:220868/142‑1 (MQ=255) tcaAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGCTTGATCATTACAGCATGAAGCTACCCa > 2:649612/1‑143 (MQ=255) tcaAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTGAGTCGGCGGAAAAGAAAGCGGAGCGTGAACGCCCCGCGATTGACCACTACAGCAACAAGCTACCCa > 1:327166/1‑143 (MQ=255) tcaAGCATAAACAACTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGCGACCGCCCCGCGACTGATCATTACAGCATGAAGCTACCCa > 1:364020/1‑143 (MQ=255) aaGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTCAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGc > 2:319029/1‑143 (MQ=255) aGCATAAACACCCCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCa < 2:983160/143‑1 (MQ=255) gCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCa < 1:1011187/142‑1 (MQ=255) gCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCa > 2:1011187/1‑142 (MQ=255) tAAACGCCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCAcgaa < 2:688560/143‑1 (MQ=255) cacCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGCTTGATCATTACAGCATGAAGCTACCCAGCAcgaccccg > 1:949198/1‑138 (MQ=255) ccgcgcgAGACCCGACGGAACCGAAAGCGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCAcgaagccgaa < 1:739400/140‑1 (MQ=255) ccTCGCTAGAACAGATGGAACCGCAAGAGAGCGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCAcgaagccgaa < 1:266535/143‑1 (MQ=255) ccTCGCAAGGACAGATGGAACCTCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCAcgaagccgaa < 2:101656/143‑1 (MQ=255) cTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCAcgaagccgaag < 2:755909/143‑1 (MQ=255) cGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGCTCCTTACAGCATGAAGCCACCCAGCAcgacaccgagcca > 1:545996/1‑139 (MQ=255) gCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTTCACGCCCcgcttt < 2:312449/100‑4 (MQ=255) gCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTTCACGCCCcgcttt > 1:312449/1‑97 (MQ=255) cAGATGGAACCGCAAGGGAGAGGTCACTATACAACGGCCGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCa < 2:423726/143‑1 (MQ=255) gagggAACCGCAAGAGAGAGGGCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAgg < 2:344317/140‑1 (MQ=255) gATGGCACCGCAACAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAgg < 2:9254/143‑1 (MQ=255) gtgaaaCCGCCAGGTAGAGGTCACCATAGAAGGGAGGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGCACGCCCCGCGATTGATCCTTACCGCCTGATCCTTCCTAGCACGAAGCCGAAGCAAACGGCCAggg < 1:312625/139‑1 (MQ=255) tGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGc < 2:560737/143‑1 (MQ=255) gCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAccc > 2:385565/1‑143 (MQ=255) gTCACTATACAACGGACGGGGGACGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCAGCGTCCGGTCAGTACAGCATGGAGCTACCCAGCACGCAGCCGAAGCAAACGGCCCGGGCAACACCCAGCGGAGCCgg > 1:305121/1‑143 (MQ=255) gTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCgg > 2:253863/1‑143 (MQ=255) aCTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGAt > 1:142024/1‑143 (MQ=255) cTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATg > 2:572531/1‑143 (MQ=255) gacggGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGAtt < 2:989355/58‑1 (MQ=255) gacggGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGAtt > 1:989355/1‑58 (MQ=255) gacggGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGAt < 1:24087/143‑1 (MQ=255) gacggGGGAAGGATGTTAGGCGCCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGAt < 1:851498/143‑1 (MQ=255) gggAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTg > 2:935059/1‑143 (MQ=255) ggAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGa < 2:194686/143‑1 (MQ=255) gATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACg > 2:303831/1‑143 (MQ=255) aTGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGGCGAAGCACGGATGGTTGAAGACGa > 1:630518/1‑143 (MQ=255) aTGTCAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGa < 1:319029/143‑1 (MQ=255) tgctagacatcggCAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGaa < 1:344928/133‑1 (MQ=255) tGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGaa > 2:834303/1‑143 (MQ=255) tGTTAGGCTGCGCGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCTGGATGAAGAACGGATGGTTGAAGACGaa < 1:410318/143‑1 (MQ=255) tAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAAttt > 2:655129/1‑143 (MQ=255) aGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTa > 1:907730/1‑143 (MQ=255) ggcggcggGAAAGAAAGCGGGGCGTGAGCGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTAc < 2:614776/141‑1 (MQ=255) gTCGGCGGGAACGACAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTAc < 2:796847/143‑1 (MQ=255) gTCGGCGGGAAAGAAGGCGGGGCGTGAACGCCCCGCGATTGATCATGACAGCATGAAGCTCCCCAGCCCGACGCCGTAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTAc < 1:763219/143‑1 (MQ=255) gaaagaaaGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACgg < 2:142024/143‑1 (MQ=255) aaagaaagCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGACCGGATGGTTGAAGACGAATTTACCGATACggg > 2:335886/1‑143 (MQ=255) atcgCGGGGCGTTCACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCACACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATCTACCGATACAGgtagt > 2:312701/4‑143 (MQ=255) tcgCGGGGCGTTCACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAGGACGAGTTTACCGATACGGgtagta > 1:685726/3‑143 (MQ=255) tcgCGGGGCGTTCACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGgtagta > 2:247889/3‑143 (MQ=255) tcgCGGGGCGTTCACGCCCCGCGATGGATGATTACCGCATGAAGCTACCCAGCAAGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGCGAACCCGGGAGGAAGAGCGGACGGTGGAAGGCGCAGATAGGGATACGGggagta > 2:259784/3‑143 (MQ=255) cGGGGCGTTCACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGCACCCGGGATGAAGAACGGACGGTCGAAGACGAATCTACCGATACGGGCAGTAccc > 1:587699/1‑143 (MQ=255) cGGGGCGTTCACGCCCCGCGATTGATCATTACAGCATGAAGCTAACCAGAACGAAGAAGAAGCAAACGGGTGGGTCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTAccc > 2:471252/1‑143 (MQ=255) | CACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCC > NC_000913/4365316‑4365587 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |