| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,164,897 | G→A | R359C (CGC→TGC) | parC ← | DNA topoisomerase IV, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,164,897 | 0 | G | A | 95.5% | 53.6 / NA | 22 | R359C (CGC→TGC) | parC | DNA topoisomerase IV, subunit A |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (6/15); minor base T (0/1); total (6/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CGACATCAGCGCCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCGCACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTC > NC_000913/3164757‑3165036 | cgACATCAGCGCCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGCGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGACGGcaca > 1:695608/1‑143 (MQ=255) gcgcCGGTTTCGGTTCATCCTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATcg < 2:837136/143‑1 (MQ=255) ttCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGGGGATATGCAGGCGCTTGAGGGCTTTCTCCAGCCGATAGTTCAGTCGCGGACACTCGGTGTCGCGGCGGACCACCCGCCAtt > 1:759735/1‑143 (MQ=255) ttCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGCCCTTCGCCAAACGATAGTTCAGTCGGCGGCACACGGTCTCGCGGCGGAGCCCCAGCCAtg > 1:116398/1‑142 (MQ=255) aaTCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCa > 1:397894/1‑119 (MQ=255) aaTCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCa < 2:397894/119‑1 (MQ=255) tCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAgg < 2:565937/143‑1 (MQ=255) agaAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCAGAGGAACACCAGCCAATCCGAGAGGGTTTACAGGAGGATGTTCACCGCCGgaca > 2:727036/1‑142 (MQ=255) tcaGCAAACCTTCGTGGCTTTGCTGGCGCTTGTGGATTTTCTCTACTCTATATTTCAGTCGGCGTCACATGTTATCGCTGCTGAACACCAGCCATTCGGAGAGGATTTCCAGTAGGTTTTTCACCGCCGGACGACCATCCAGa < 1:563038/142‑1 (MQ=255) aaaCCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGAt < 2:330825/143‑1 (MQ=255) tggATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAg < 1:325471/142‑1 (MQ=255) cgtatatGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGTCTCACGTTATCGCAGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAg < 1:818812/140‑1 (MQ=255) aGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAg < 2:880909/143‑1 (MQ=255) ggATATGCAGGCGCTTGAGGAGTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAgg < 2:264669/143‑1 (MQ=255) gcgcTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGAt < 2:889793/143‑1 (MQ=255) gAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGGTTCTCACCGCCGGACGACGGTCCGGAGCGACGAGACAAAGGCTAATGCGCTAGctcg > 1:442999/1‑142 (MQ=255) ggACTTTCTCCAGACGATAGTTCAGTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCttt < 2:116398/143‑1 (MQ=255) gTTCAGTCGGCGGCACACGGTAGCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGa < 2:458544/143‑1 (MQ=255) ttCCGTCGGCGTCACACGGTATCGCGTCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAATGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGaa < 1:1011610/143‑1 (MQ=255) tccatccggcggCAGACGTTACCGCGGGGGACCAACAGCCATTCGCAGAGGATTTCCAGCAGGTGTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGaa < 1:642610/137‑1 (MQ=255) gTCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAgg < 2:917116/143‑1 (MQ=255) tCGGCGGCATACGGGATCGCGGCGGAGCACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAggt < 1:742547/143‑1 (MQ=255) tCGGCGGCACACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATGCGATGGCTCTTTGCCAGATCGGTGGCGGCGCAGCggt > 1:360772/1‑143 (MQ=255) gcggcaCACGGGATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAggtggt < 2:314918/143‑1 (MQ=255) ggcaCACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGGTTCTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtc < 2:846838/143‑1 (MQ=255) | CGACATCAGCGCCGGTTTCGGTTCATCTTCATTACGAATGATCTCAATCACTTCGTCGATATTGAGAAACGCCACCAGCAAACCTTCGAGGATATGCAGGCGCTTGAGGACTTTCTCCAGACGATAGTTCAGTCGGCGGCGCACGGTATCGCGGCGGAACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTC > NC_000913/3164757‑3165036 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |