Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,532,766 Δ1 bp 11.1% coding (419/747 nt) yjhP ← putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,532,7650T.11.1% 83.5 / 7.1 36coding (420/747 nt)yjhPputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (16/13);  new base . (2/2);  total (20/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.00e-01

GGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAG  >  NC_000913/4532522‑4533032
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                           
ggCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCCGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGCCGCCCGTAGGGTTCGCCGTTAGGCATGATCCCGCCCGGTTTcc                                                                                                                                                                                                                                              >  1:137855/1‑274 (MQ=255)
  cccTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCCCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGCAGCCGCAGCCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGATGGGTTCGCCGATCAGCCATATCCCCCCCGGTTTCAg                                                                                                                                                                                                                                             >  1:273807/1‑274 (MQ=255)
                                     gCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCCGGCTTTGCGCTAGCCGCCCCTCCGCCCCGGCAAAccc                                                                                                                                                                                                         >  1:70453/1‑275 (MQ=255)
                                                                                 ccGGTCCCATCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATGTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTTCGCTGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACAtt                                                                                                                                                             <  2:137855/275‑1 (MQ=255)
                                                                                    gTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAAccc                                                                                                                                                                                                         >  2:291674/1‑228 (MQ=255)
                                                                                        cagtcgtCCTGGTCGGCCATACCCATTGCCCCCACGCCATAGCCGGGATCGTCGAACGCTCCGACAAGTCCTGTCAGCGTCAGGAATTCGCTTGTCGGGCGGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCAtt                                                                                                                                                      <  1:45244/269‑1 (MQ=255)
                                                                                        cAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCg                                                                                                                                                                                                     <  1:291674/228‑1 (MQ=255)
                                                                                               ccTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGATCTGACGCCCCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGCCGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTTGCAGCTCCTCCGCCCCGGCAAACCCACCGCAAATCCATGTAGAGCTGACGCCGGCCCCCCCGCCCCACTTTCACTTTGCGAc                                                                                                                                                >  2:45244/1‑274 (MQ=255)
                                                                                               ccTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCCACCCCGGCCGCCCCGCCACATTTTTCATTTGCCAc                                                                                                                                                >  1:173337/1‑274 (MQ=255)
                                                                                                           gtggcATATCCACCGCGGCATAGCCGAGTGCGTCCGACGCACCGAAAAGGCCTCGCAGCGTCAGGGAATGGCTGGTCGGGCTAACGCCGCAGGCCTGGCCAATTTCTTAGTTTGCTGGAAGCTGAGGCCAGTAAGGTTCGCGGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTACGCCGCCCGGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCGGCCACGTCACATTTTTCATTTGGGACGTAACCGGCCGCa                                                                                                                                   <  1:43546/271‑1 (MQ=255)
                                                                                                                 tttCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCAACGTCACCTTTTTCATTTGCGACGTAACCGCCCGCATCGTTa                                                                                                                             >  2:245159/1‑275 (MQ=255)
                                                                                                                      cccacGTCAGAGCCCATGTCGTCGAAGCCTCCGACAAGTCCTGGCGGGGTCAGAAAATGTGTTGTCGAGCTGACGCCGCCGGCCTGGGCTATTGCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGaa                                                                                                                         <  1:53706/273‑1 (MQ=255)
                                                                                                                         acGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTTAGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGCCACATTTTCCATTTGCGACGTAACCGGc                                                                                                                                       >  2:290379/1‑257 (MQ=255)
                                                                                                                             cATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTCCATTTGCGACGTAACCGGCCGCa                                                                                                                                   <  1:290379/257‑1 (MQ=255)
                                                                                                                               tAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACCCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGGTTCAGGCTTTGCGCTAGCAGCTCCTCTGCCCCGGCAAAACCACCGGCAATCCATGTCGCGCCGACGAAGGCCGCCAAGTCCCCATCTTCATTTGCGACGTAACCGGCAGCATCGTTATGAATAAAATGTa                                                                                                                >  2:238340/1‑274 (MQ=255)
                                                                                                                                       aTCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCCCATTTTTCATTTGCGACGTAACCGGCCCCATCGTTCTGAATACAATGTAACCGTTc                                                                                                         >  1:299111/1‑273 (MQ=255)
                                                                                                                                           tcgAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCCTTTCGCGCCGCCGCAGGCCCCCACGTACCATTTTTCCTTTGCGACGTAACCGGCCGCTTCCTTTGGAATAAAATGTACGGGTTCGCTGa                                                                                                    >  1:225492/1‑274 (MQ=255)
                                                                                                                                                               tGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCACACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGCCACATTTTTCATTTGCGACGTACCCGGCCGCATCGTTTTGAATAAAATGCACGCTTTCCCTGCAGCCGAGTTCTTCCGCGCGAcc                                                                              >  1:230184/1‑274 (MQ=255)
                                                                                                                                                               tGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACg                                                                              >  1:166882/1‑275 (MQ=255)
                                                                                                                                                                   gtcGTCAGGGAATGGTTTTTCGGGCTGACGCCGCAGGCCTGGGCTATTTCTGCTGTTGCGGGTAGCTGACGCCAGTAGGGTTGGCCTATAAGCATGATCCCGCCCGGTTTCAGGCTGTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAAGCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCtt                                                                           <  1:254038/273‑1 (MQ=255)
                                                                                                                                                                             aaTCGCTTGTCCGGCTGACCCCGCAGGCCTGGGCTATTTCTTCGGTTGCTGGTAGCTGCCGCCAGTAGGGTTCTCCGATAAGCATGAGCCCGCCCTGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGcgcg                                                                 <  2:225492/275‑1 (MQ=255)
                                                                                                                                                                                cGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCAtt                                                                                                                                                      >  2:104209/1‑187 (MQ=255)
                                                                                                                                                                                    tGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCg                                                                                                                                                  <  1:104209/187‑1 (MQ=255)
                                                                                                                                                                                     gTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCCCGGTGaaaac                                                         >  1:25028/1‑272 (MQ=255)
                                                                                                                                                                                            ncntctgatgtttaaaagggacgagttctGTTGCTGGTAGCGGACGCCAGTAGGGCTCGCCGTTAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACAGTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTc                                                  <  1:306883/250‑1 (MQ=255)
                                                                                                                                                                                                      ggCCTGGGCTATTTGTGCTGTTGCTGGTAGCTGCCGCCAGTAGGGTTCGCCCATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGc                                        <  1:245159/275‑1 (MQ=255)
                                                                                                                                                                                                                 tttCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGACGAGCGACCTCATGTCGATGCCCGTcccc                                >  1:152038/1‑272 (MQ=255)
                                                                                                                                                                                                                 tttCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGCTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCTCGCCGACGCAGGCCGCCACGTCACATTTTTCCTTTTCGACGTAACCGGCCGCCTCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTGTTCCGCCCGACGCTTTCCCTGCGCGGTTGAGAGCACACTCCAGTCAATGCCATGCCGCGTa                             >  1:108944/1‑275 (MQ=255)
                                                                                                                                                                                                                               ggTAGCTGACGCCAGTAGGGCTAGCCGATAAGCATGATCCCCCCCGGTTTCAGGCTTTGCGCTAGCCGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGGCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCCTTGCCTGCGCGGTGACGAGCGa                                                      >  2:306883/1‑236 (MQ=255)
                                                                                                                                                                                                                                  cgaacccGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATc                                                                                                                                 >  2:112840/7‑158 (MQ=255)
                                                                                                                                                                                                                                    cTGACGCCAGTAGGGTTGGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGccc          <  2:25028/275‑1 (MQ=255)
                                                                                                                                                                                                                                      cccGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTa                                                                                                                             <  1:112840/156‑1 (MQ=255)
                                                                                                                                                                                                                                          ccAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGa                    >  2:99268/1‑259 (MQ=255)
                                                                                                                                                                                                                                           cAGTAGGGTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTCGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATTAATAAAACGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCag  <  2:230184/275‑1 (MQ=255)
                                                                                                                                                                                                                                            aGTAGGGTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCag  <  2:166882/274‑1 (MQ=255)
                                                                                                                                                                                                                                              tAGGGTTCGCCGTTAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAACCCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATccc                <  1:99268/259‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                           
GGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGTTCGCCGATAAGCATGATCCCGCCCGGTTTCAGGCTTTGCGCTAGCAGCTCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAG  >  NC_000913/4532522‑4533032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: