Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,296,190 A→C 38.2% intergenic (+397/+247) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,1900AC38.2% ‑1.7 / 8.4 16intergenic (+397/+247)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base A (7/3);  new base C (4/2);  total (11/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.01e-01

TAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAA  >  NC_000913/4295917‑4296460
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                              
tAAGGCGTTTATGCCGCATCCGACAGCAAAGCCTGAGGCAAGTCTTAACGCGTCTTATCAGGCCTACGCAAGACAGCGCAATAGCCTGATTTAGAGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCAACCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa                                                                                                                                                                                                                                                                                <  2:325946/274‑1 (MQ=255)
    gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGt                                                                                                                                                                                                                                                                           <  2:232096/275‑1 (MQ=37)
         tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACACCCACGCCTGATGCGACCCTTAACGCGTCTTAt                                                                                                                                                                                                                                                                      >  1:49338/1‑275 (MQ=37)
                 aTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATGCGACATCAACGCCTGATGCGACGCTTGCGGCGTCTTATCAGGCCTACGCCAGACCGCGCAATAGCCTGATTTAGCGTCATTTTGTCGGGCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGAAGCTTAACGCGTACTATCCGGCCTa                                                                                                                                                                                                                                                              >  2:187983/1‑275 (MQ=11)
                                                                       gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACCCTAGACAGAGCAATAGCCTGATTTAGCGTGATTCTGTAGTTCGGATAAgga                                                                                                                                                                                                         >  1:75135/1‑273 (MQ=16)
                                                                                    ccttgtttaggcttaTTTTGTAGGTCGGGTAAGGCATGTAAGCCGGATCCGACTGCAACGCCGGAGGCGACGCTTGCCGCGTCTGAGCAGGCCTAAGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTGTGTAGGTAGGGTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATc                                                                                                                                                                                           <  1:105750/261‑1 (MQ=11)
                                                                                    ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGTCGTTTATGCCGCATCCGACATCAACGACTGATGCAACGCTTAACGCGTATTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGCTTTTGTAGGTCGGATAAGACGTTTAcgccgc                                                                                                                                                                                              >  2:297360/1‑272 (MQ=12)
                                                                                      tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAGTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGGCTTATCAGGCCTACACCATACAGCGCAATAGCCTGATTTAGCGTGCTTATGTAGGTCGGATCAGGCGTTTACGCCGAATCCg                                                                                                                                                                                         >  2:360906/1‑275 (MQ=255)
                                                                                                                          tACGCCGCATCCGACATCAACGCCTGATGCGACGGTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATCTACCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAGGCGACGCTTAACGCGTCTTTTCAGGCCTCCGCTATACACCGCATTAGCCTGATTTACCGTGATTTTGTCGGTCGGACAAGGCCTTTACGCCGCATCCGACATCAATTCTTAATCCGACCCTTCACGCGCCTTAt                                                                                                                                                     >  1:381422/1‑275 (MQ=11)
                                                                                                                                                                                                                                    ggtgtctgcgatgggtagagtgcccagcGCCTGATGCGACGCCTGACTCTTCTTATCAGGCCTACGCCAGACAACGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATGCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAATGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgc                                           <  2:324688/248‑1 (MQ=11)
                                                                                                                                                                                                                                          ttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCTCATCCGACATCAATGCCTGATGCGACCCTTGCCGCTTCTTa                                     >  1:365974/1‑275 (MQ=9)
                                                                                                                                                                                                                                                cGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                               <  2:292168/275‑1 (MQ=17)
                                                                                                                                                                                                                                                 gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCCTTTACGCCCCATCCGACACCACTGCCTGATGCACCGCTTGCCGCGTCTTATCAGGcc                              >  1:228710/1‑275 (MQ=255)
                                                                                                                                                                                                                                                            caccGCCTGATGCGACTTTTGCCGCGTCTTATCATGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGGTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCg                   <  2:227956/272‑1 (MQ=255)
                                                                                                                                                                                                                                                                     aTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATACGACGCTTGCCGCGTCTTATACGGCCTCTCATAACCGTTGGTTAAt          >  1:337618/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                      tGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACTCTTGCCGCCTCTTATCAGGCCTTTCTTAACCGTTGGTTAAtt         >  1:60631/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                           cGCTTGCCGCGTCTTATCAGGACTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGGGTTTTATCAGGACTATCTTAACCGTTGGTTAATTTTTc     >  2:4869/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                             attgaCGGGTCGTTTCAGCCCGTCGCCAAACGGCGCAATACCCTGATTTAGCGGGTTTTTGTAGGTCGGATGAGGCGTTTCTGCCGCGTCAGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa  <  1:142162/271‑1 (MQ=14)
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                              
TAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAA  >  NC_000913/4295917‑4296460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: