Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,730,008	A→G	8.8%	F253L (TTC→CTC)	xylA ←	D‑xylose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,730,008	0	A	G	8.8%	121.4 / 4.2	46	F253L (TTC→CTC)	xylA	D‑xylose isomerase
Reads supporting (aligned to +/- strand):  ref base A (22/19);  new base G (2/2);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.30e-01

ACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCT  >  NC_000913/3729736‑3730275                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                             acgTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCAAGCGTCGCGTGGTTAGCTTCAATTTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTCAAGGAAGc                                                                                                                                                                                                                                                                            >  1:238606/1‑275 (MQ=255)                 aaGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTACTCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTcg                                                                                                                                                                                                                                                                >  1:138158/1‑271 (MQ=255)                 aaGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTcg                                                                                                                                                                                                                                                                <  2:138158/271‑1 (MQ=255)                 aaGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑gg                                                                                                                                                                                                                                                            <  2:25660/274‑1 (MQ=255)                              cGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGCCCGTCGC‑GGCACCGTAATCatat                                                                                                                                                                                                                                              >  2:384435/1‑275 (MQ=255)                                           gCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTTTTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTACCTTCAATGTTCCGTTTAATCCCTTTTTCCAGACCAACCCGTTTCAGGAATCCATAGACCGTCGCTGCCATC‑TAAACATATTGATGTTTGTTc                                                                                                                                                                                                                                  >  1:361873/1‑274 (MQ=255)                                                                   cACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGCTTAATCTATTTTTCCAGACACAACTGTTTCAGGACGCCATCAAACGTACC‑GGCATCGTAATCATATTGATGTTTGGTTCGTTCTTGTGGTTTCGGTTAGAtca                                                                                                                                                                                                         >  2:190728/1‑273 (MQ=255)                                                                              tctcTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGt                                                                                                                                                                                                                                                      >  1:156830/1‑219 (MQ=255)                                                                                  ttCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATc                                                                                                                                                                                                                                                  <  2:156830/219‑1 (MQ=255)                                                                                   tCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTTAGGAGGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGaa                                                                                                                                                                                         >  2:13697/1‑275 (MQ=255)                                                                                    ccACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGTTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGaaa                                                                                                                                                                                        <  2:305967/275‑1 (MQ=255)                                                                                      tcggtaatgtgtctaagagacaggccccagcccagGTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTTGCTATTTCATGATGGAAAGAGTGACCTGCCAGCTTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGaaa                                                                                                                                                                                        <  2:368700/248‑1 (MQ=255)                                                                                        acTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCCATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCCGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGCCCGTCGC‑GGCATCGTCATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCCGTTCGATCAGCAACGTGCCCTGGGAAcca                                                                                                                                                                                     >  2:325247/1‑273 (MQ=255)                                                                                            ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGGGCCCTGGACACCGAttt                                                                                                                                                                                 >  1:53576/1‑274 (MQ=255)                                                                                                 gAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCCTCGTAATCATATTGATGTTTGGTCGGTTCTTCCGGTTTCGGTTCGATAAGCCACGTGCCCTTGAAACCGTTtttttgtt                                                                                                                                                                           >  1:12297/1‑275 (MQ=255)                                                                                                      ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTATTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACAGATtttatgtttattc                                                                                                                                                                      >  2:133246/1‑273 (MQ=255)                                                                                                      ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATAGTCATCATATTTATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAAAGTTCCCTGGAAACCGATtttatgtttctt                                                                                                                                                                       >  2:174244/1‑271 (MQ=255)                                                                                                            tgtCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAacc                                                                                                                                                                <  2:153969/275‑1 (MQ=255)                                                                                                                        gTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTg                                                                                                                                                                                            >  1:368700/1‑235 (MQ=255)                                                                                                                        gTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGTCCAACCACCCTCTCCATa                                                                                                                                                    >  1:339968/1‑275 (MQ=255)                                                                                                                              cATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAGGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAg                                                                                                                                                                                                        >  2:153184/1‑217 (MQ=255)                                                                                                                                  gCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAGGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAAc                                                                                                                                                                                                    <  1:153184/217‑1 (MQ=255)                                                                                                                                                        ccGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAg                                                                                                                                                                                                                                                                       >  2:208342/1‑129 (MQ=255)                                                                                                                                                            aCAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCg                                                                                                                                                                                                                                                                   <  1:208342/129‑1 (MQ=255)                                                                                                                                                                        ctatggacgttgCTATTTCAGGATGGAAAGAGTGTCCTGCCAGCGTCGCGTGGTTAGCTTTAATGTTCAGTTTACTCTCTTTTTCCAGACCAACCTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGc                                                                                                    <  2:304891/264‑1 (MQ=255)                                                                                                                                                                        cAATTGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGTTTATCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGTAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACa                                                                                                      <  2:239023/273‑1 (MQ=255)                                                                                                                                                                          aTGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATTTGCATAAAGCGGACACGTTGTTCACGATCCTGACTCAAAGCGGGATTTAACa                                                                                                      >  1:239023/1‑271 (MQ=255)                                                                                                                                                                          aTGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTAGC‑AGCATCGTAATCATATTTATGTTTGGTCGGTTCTTGCGGTTTCGGTTAGATAAGCAAAGTGCACTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGGACAGTTGTTAACCCTCCTGAAGAAAGTTGGTGTTTAACAGCGt                                                                                                  >  2:234692/1‑275 (MQ=255)                                                                                                                                                                                            ggagggACAGAGTGACCTGCCAGCGGTGCGGTGTTAGCTTCACTGTTCAGTTTAATCTCTTTTTCCCGACCAAACTGTTTCAGGAAGCCATAGACCAGCGC‑GGCATCGTAATCATTTTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGAc                                                                                 <  1:384435/270‑1 (MQ=255)                                                                                                                                                                                              atgGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTTAGGAGGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCg                                                                               <  1:13697/274‑1 (MQ=255)                                                                                                                                                                                                    agagTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCcaca                                                                        <  2:339968/275‑1 (MQ=255)                                                                                                                                                                                                        tGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCca                                                                          <  1:56628/269‑1 (MQ=255)                                                                                                                                                                                                        tGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCca                                                                          >  2:56628/1‑269 (MQ=255)                                                                                                                                                                                                         gcccTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACa                                                                   <  2:12297/273‑1 (MQ=255)                                                                                                                                                                                                         gagcTGCCAGNGTGNGNNGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATGGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCt                                                                                                                                                                     <  1:325247/174‑1 (MQ=255)                                                                                                                                                                                                                                 cTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCACTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTAt                                             >  2:232839/1‑273 (MQ=255)                                                                                                                                                                                                                                     aaTGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCCACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCAc                                                         >  2:90696/1‑257 (MQ=255)                                                                                                                                                                                                                                     aaTGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATtttatg                                                                                                                                                                             >  2:167240/1‑141 (MQ=255)                                                                                                                                                                                                                                         ttCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATtttatgttta                                                                                                                                                                         <  1:167240/141‑1 (MQ=255)                                                                                                                                                                                                                                         ttCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGcc                                                     <  1:90696/257‑1 (MQ=255)                                                                                                                                                                                                                                                   tctcTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTc                                                                                                                                                                                                              >  1:220274/1‑94 (MQ=255)                                                                                                                                                                                                                                                       tttttCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATa                                                                                                                                                                                                          <  2:220274/94‑1 (MQ=255)                                                                                                                                                                                                                                                               gACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCcaca                                                                        >  1:276279/1‑216 (MQ=255)                                                                                                                                                                                                                                                                aCCCAACTGGTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATGGATGTTTGGTCGGTTCTTGCTGTTTCGGTTCGATAAGCAACGTGCCATGGTAACCGATGTTATGTTTATGCTCAACCACCATCTGCATAAAGCGACCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCAACAGGACATAGTTTTCACCGCCCAATTTGTGGGTTGCTTCCATCGCTGTAACAACTTGCGTTg            <  2:381368/275‑1 (MQ=255)                                                                                                                                                                                                                                                                   aaaCTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGAc                                                                    <  2:276279/216‑1 (MQ=255)                                                                                                                                                                                                                                                                          ttCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCTACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTCTGGTTTGCTTCACTCGCTGTAACAACTTCCGTTGCCCCCCAGCt  >  1:148272/1‑275 (MQ=255)                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                             ACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGC‑GGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCT  >  NC_000913/3729736‑3730275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑