Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,730,008 A→G 8.8% F253L (TTC→CTC)  xylA ← D‑xylose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,730,0080AG 8.8% 121.4 / 4.2 46F253L (TTC→CTC) xylAD‑xylose isomerase
Reads supporting (aligned to +/- strand):  ref base A (22/19);  new base G (2/2);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.30e-01

ACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCT  >  NC_000913/3729736‑3730275
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                            
acgTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCAAGCGTCGCGTGGTTAGCTTCAATTTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTCAAGGAAGc                                                                                                                                                                                                                                                                            >  1:238606/1‑275 (MQ=255)
                aaGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTACTCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTcg                                                                                                                                                                                                                                                                >  1:138158/1‑271 (MQ=255)
                aaGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTcg                                                                                                                                                                                                                                                                <  2:138158/271‑1 (MQ=255)
                aaGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCgg                                                                                                                                                                                                                                                            <  2:25660/274‑1 (MQ=255)
                             cGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGCCCGTCGCGGCACCGTAATCatat                                                                                                                                                                                                                                              >  2:384435/1‑275 (MQ=255)
                                          gCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTTTTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTACCTTCAATGTTCCGTTTAATCCCTTTTTCCAGACCAACCCGTTTCAGGAATCCATAGACCGTCGCTGCCATCTAAACATATTGATGTTTGTTc                                                                                                                                                                                                                                  >  1:361873/1‑274 (MQ=255)
                                                                  cACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGCTTAATCTATTTTTCCAGACACAACTGTTTCAGGACGCCATCAAACGTACCGGCATCGTAATCATATTGATGTTTGGTTCGTTCTTGTGGTTTCGGTTAGAtca                                                                                                                                                                                                         >  2:190728/1‑273 (MQ=255)
                                                                             tctcTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGt                                                                                                                                                                                                                                                      >  1:156830/1‑219 (MQ=255)
                                                                                 ttCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATc                                                                                                                                                                                                                                                  <  2:156830/219‑1 (MQ=255)
                                                                                  tCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTTAGGAGGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGaa                                                                                                                                                                                         >  2:13697/1‑275 (MQ=255)
                                                                                   ccACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGTTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGaaa                                                                                                                                                                                        <  2:305967/275‑1 (MQ=255)
                                                                                     tcggtaatgtgtctaagagacaggccccagcccagGTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTTGCTATTTCATGATGGAAAGAGTGACCTGCCAGCTTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGaaa                                                                                                                                                                                        <  2:368700/248‑1 (MQ=255)
                                                                                       acTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCCATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCCGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGCCCGTCGCGGCATCGTCATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCCGTTCGATCAGCAACGTGCCCTGGGAAcca                                                                                                                                                                                     >  2:325247/1‑273 (MQ=255)
                                                                                           ttCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGGGCCCTGGACACCGAttt                                                                                                                                                                                 >  1:53576/1‑274 (MQ=255)
                                                                                                gAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCCTCGTAATCATATTGATGTTTGGTCGGTTCTTCCGGTTTCGGTTCGATAAGCCACGTGCCCTTGAAACCGTTtttttgtt                                                                                                                                                                           >  1:12297/1‑275 (MQ=255)
                                                                                                     ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTATTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACAGATtttatgtttattc                                                                                                                                                                      >  2:133246/1‑273 (MQ=255)
                                                                                                     ggTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATAGTCATCATATTTATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAAAGTTCCCTGGAAACCGATtttatgtttctt                                                                                                                                                                       >  2:174244/1‑271 (MQ=255)
                                                                                                           tgtCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAacc                                                                                                                                                                <  2:153969/275‑1 (MQ=255)
                                                                                                                       gTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTg                                                                                                                                                                                            >  1:368700/1‑235 (MQ=255)
                                                                                                                       gTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGTCCAACCACCCTCTCCATa                                                                                                                                                    >  1:339968/1‑275 (MQ=255)
                                                                                                                             cATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAGGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAg                                                                                                                                                                                                        >  2:153184/1‑217 (MQ=255)
                                                                                                                                 gCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAGGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAAc                                                                                                                                                                                                    <  1:153184/217‑1 (MQ=255)
                                                                                                                                                       ccGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAg                                                                                                                                                                                                                                                                       >  2:208342/1‑129 (MQ=255)
                                                                                                                                                           aCAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCg                                                                                                                                                                                                                                                                   <  1:208342/129‑1 (MQ=255)
                                                                                                                                                                       ctatggacgttgCTATTTCAGGATGGAAAGAGTGTCCTGCCAGCGTCGCGTGGTTAGCTTTAATGTTCAGTTTACTCTCTTTTTCCAGACCAACCTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGc                                                                                                    <  2:304891/264‑1 (MQ=255)
                                                                                                                                                                       cAATTGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGTTTATCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGTAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACa                                                                                                      <  2:239023/273‑1 (MQ=255)
                                                                                                                                                                         aTGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATTTGCATAAAGCGGACACGTTGTTCACGATCCTGACTCAAAGCGGGATTTAACa                                                                                                      >  1:239023/1‑271 (MQ=255)
                                                                                                                                                                         aTGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTAGCAGCATCGTAATCATATTTATGTTTGGTCGGTTCTTGCGGTTTCGGTTAGATAAGCAAAGTGCACTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGGACAGTTGTTAACCCTCCTGAAGAAAGTTGGTGTTTAACAGCGt                                                                                                  >  2:234692/1‑275 (MQ=255)
                                                                                                                                                                                           ggagggACAGAGTGACCTGCCAGCGGTGCGGTGTTAGCTTCACTGTTCAGTTTAATCTCTTTTTCCCGACCAAACTGTTTCAGGAAGCCATAGACCAGCGCGGCATCGTAATCATTTTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGAc                                                                                 <  1:384435/270‑1 (MQ=255)
                                                                                                                                                                                             atgGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTTAGGAGGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCg                                                                               <  1:13697/274‑1 (MQ=255)
                                                                                                                                                                                                   agagTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCcaca                                                                        <  2:339968/275‑1 (MQ=255)
                                                                                                                                                                                                       tGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCca                                                                          <  1:56628/269‑1 (MQ=255)
                                                                                                                                                                                                       tGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCca                                                                          >  2:56628/1‑269 (MQ=255)
                                                                                                                                                                                                        gcccTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACa                                                                   <  2:12297/273‑1 (MQ=255)
                                                                                                                                                                                                        gagcTGCCAGNGTGNGNNGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATGGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCt                                                                                                                                                                     <  1:325247/174‑1 (MQ=255)
                                                                                                                                                                                                                                cTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCACTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTAt                                             >  2:232839/1‑273 (MQ=255)
                                                                                                                                                                                                                                    aaTGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCCACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCAc                                                         >  2:90696/1‑257 (MQ=255)
                                                                                                                                                                                                                                    aaTGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATtttatg                                                                                                                                                                             >  2:167240/1‑141 (MQ=255)
                                                                                                                                                                                                                                        ttCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATtttatgttta                                                                                                                                                                         <  1:167240/141‑1 (MQ=255)
                                                                                                                                                                                                                                        ttCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGcc                                                     <  1:90696/257‑1 (MQ=255)
                                                                                                                                                                                                                                                  tctcTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTc                                                                                                                                                                                                              >  1:220274/1‑94 (MQ=255)
                                                                                                                                                                                                                                                      tttttCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATa                                                                                                                                                                                                          <  2:220274/94‑1 (MQ=255)
                                                                                                                                                                                                                                                              gACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCcaca                                                                        >  1:276279/1‑216 (MQ=255)
                                                                                                                                                                                                                                                               aCCCAACTGGTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATGGATGTTTGGTCGGTTCTTGCTGTTTCGGTTCGATAAGCAACGTGCCATGGTAACCGATGTTATGTTTATGCTCAACCACCATCTGCATAAAGCGACCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCAACAGGACATAGTTTTCACCGCCCAATTTGTGGGTTGCTTCCATCGCTGTAACAACTTGCGTTg            <  2:381368/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                  aaaCTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGAc                                                                    <  2:276279/216‑1 (MQ=255)
                                                                                                                                                                                                                                                                         ttCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCTACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTCTGGTTTGCTTCACTCGCTGTAACAACTTCCGTTGCCCCCCAGCt  >  1:148272/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                            
ACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCGCGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGGTTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCATCGCTGTAACAACTTGCGTTGCCGCCCAGCT  >  NC_000913/3729736‑3730275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: