Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 375,075 | G→A | 8.0% | intergenic (+194/‑384) | mhpE → / → mhpT | 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 375,075 | 0 | G | A | 8.0% | 140.1 / 2.4 | 59 | intergenic (+194/‑384) | mhpE/mhpT | 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter |
Reads supporting (aligned to +/- strand): ref base G (31/23); new base A (3/2); total (34/25) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCG‑GCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCG‑TCGCATCAGGCATCTGCACACGACTGCCGGATGC > NC_000913/374804‑375342 | cTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGa < 2:252205/274‑1 (MQ=255) ggCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATAAGGCGTTCACGCCGCATCCGGCCTACGGGTGGCGCGAGAAttt > 2:103088/1‑275 (MQ=255) aCGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGGGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGCTGGCACGGGAATTTGGAgg > 1:208642/1‑275 (MQ=255) cGGATGGTGGGCGGCCGGGGGGATATGGTCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCt < 2:388218/274‑1 (MQ=255) tgggcggcCATGAGGATATGATCGTTGTCGTGGCGCTAGATCTGCTGAACAACAATTAATGATGACGGCCGAGAACGTGAATGTTGTATGCCCATCCCAGCACGACTGCCGGATGCGTCGTGAACGCCTGATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGa < 1:142026/272‑1 (MQ=18) cgtccaggtgagtatGATGGTTGGCGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATGTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCTTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑t < 1:253541/263‑1 (MQ=255) gcCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTCGCGTCGCATCATGCATCTGCGCACGCCTGCCGGCTGCGGCGTGAACGACATGTCCGGCCTACGGAGTGCCCggggagtcgggtggcgtgtcaggtgcgtgc > 2:133600/1‑248 (MQ=21) tatGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCAta > 2:279413/1‑274 (MQ=255) tCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTCTCCGGCCTACGGATGGCGCGGGAAGTTGTAAGCCTGATAAGACGCG‑TTAGCGTTGAACCAGGc > 1:90004/1‑274 (MQ=255) tCGGTGACGTGGCGCTGGATCTGCGCAACAACAAAGAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGGTAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGc < 1:389089/274‑1 (MQ=255) gTTGAGGTGGCGCTGGATCTGAGCAACAACAAATAAGGGTGACTGGCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAAGTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCAt < 2:191405/274‑1 (MQ=255) ttGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCt > 1:369937/1‑275 (MQ=255) ttGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCATGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCCGGCATCTGCGCACGACTGCCGGCTGCGGCGGGAACGCCTTATCCGGCCTACGGGTGGCGCGAGCATTTGTAGGCCTGATAAGACGCG‑TTAGAGTCGTATCAGGCGTCt > 1:214975/1‑275 (MQ=18) tGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTg > 1:414054/1‑275 (MQ=255) gcgcAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGGGGACGACTGCCGGATGCGGCGGGAACGCCTTATCCGGCCTACGGGTGGAGGGAGACTGTGTAGGCCTGATAAGGCGCG‑GTGGCGTCGCCTCAGGCGTCTGCGCACCACTGCCGGCTgc > 1:386603/1‑275 (MQ=17) gcgcAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAAAGCCTTATCCGGCCTACGGCTGGCGCGGGAATTTGTAGGCCTTGTAAGACGCT‑GTTGCGTCGCCTCAGGCATCTGGCCACGACTTCCGGCTgc > 1:343977/1‑275 (MQ=18) gcgcAACAACAAAGAATGATGACTGCCGAGAGCGTGGATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TGAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATgc < 1:355647/275‑1 (MQ=255) aataatGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAg > 1:285077/1‑239 (MQ=255) atgatgACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCAt < 2:285077/239‑1 (MQ=255) tgatgaCTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCCCGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCGACGGATGGCGCGGGAAGTTGTAGGCCTGATAAGACGCG‑TTAGCGTGGCATCAGGCATCTGCGCAGGACTGCCAGATGCGGCGGGAACGCCTTAt > 1:136946/1‑275 (MQ=255) tgatgaCTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGCTGCGGCGTGAACGCCTTATCCGGCCTACGGCTGGCGCGGGAATTTGTAGGCCTGATAAGAACCG‑TTAGCGTCGCATCAGGCATCTGCGCACGCCTTCCGAATGCGGCGTGCACGCAtttt > 2:207608/1‑273 (MQ=17) tgatgaCTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATTCGGCGTGAACCCCTTATCCGGCCTACGGATGGCGCGGGGATTTGTGGGCCCGATAGGCCGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGGAgcggcgctacgcccttat > 1:229680/1‑263 (MQ=17) tgatgaCTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCCGGCATCTGCGCACGACTGCCGGATGCGGCGGGAACGACTTAt > 1:448782/1‑275 (MQ=255) gaggaCTGCCGAGAACGTGCATTTTTTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTAGCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCCTCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTAt < 1:274215/271‑1 (MQ=255) atgaCTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATc < 1:279413/274‑1 (MQ=255) atgaCTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATAAGGCGTTCACGCCGCATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATc < 1:103088/274‑1 (MQ=255) ggcggccagggaCGTGCATTTGGTATGCCCGTCCCAGCACGACGGGCGGATTCGGCGTGAACGCCTTCTCCGGCCTACGGATGGCGTGAGAAGTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGccatccggca < 2:369936/264‑9 (MQ=14) tGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGGGAACGCCTTATCCGGCCTACCGATGGCGCGGGAATTTGTAGGGCTGATAAGGCGCG‑TTAGCGTCCCATCAGGCATCTGCCCGCGACTGCCGGGAGCGGGCTGAAGCCCCTATCCCGccg > 2:99112/1‑274 (MQ=17) agaACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGCCGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGCACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGAG‑TTCGCGTCGCATCTGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCGTCACCGGccgtcggt > 1:220079/1‑269 (MQ=17) gatccgaagacgcgtgccgagtcgggcgTGAACGCCTTATCCGGCCGACGGAGGGCGGGAGGAGTTGGAGGTCTGATAAGGGGCGTTAGCGTCGCATCAGGCATCGGCGCACGACTGCCGGATGCGGCGGGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGc < 2:343976/250‑1 (MQ=11) cATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGcc > 1:124292/1‑275 (MQ=255) cACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTTCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGCATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGCTGAGGCGTGAACGCCTTGACCAGCCTACGGGTGGCGCGAGCCTTTGTAGGCCTGGTacgc > 2:115771/1‑272 (MQ=14) aCGGCTGCGGGATGCGGCGGGAACGCCTTATCCGGCCTACGGAGGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGa < 1:425882/274‑1 (MQ=255) cTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGa > 2:327086/1‑210 (MQ=38) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCCGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGGTAAGACGCGTTAg > 1:380896/1‑275 (MQ=255) ggATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTTCGACCGACTGCCGGATGCGGCGTGCAGGCCTTATCCGCCCTACGGGTGGCGCCAGAATTTGTAGGCCTTATTAGCCGCGTTAg > 2:182590/1‑273 (MQ=14) gagggggggggacggCCTTATCCGGCCTGCGGATGGGGGGAGAATTGGTAGGTGTGGTAGGACGCGTGATGGGCGCATCAGGCAGCTGCGCAGGACTGCCGGAGGCGGCGTGAACGCCTTATCCGGCCTACGGAGGGCGCGGGAATTTGTAGGCCGGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGt < 2:490016/261‑1 (MQ=11) ttATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGCAACCCGTATCCGGCCCACTGGTGGCGCGCGCATTTGTAGGCCTGATAAGCAGCCTTCGCGTCCCATCCGGCCTCTggg > 2:171567/1‑273 (MQ=11) tccggcggatgggttgCGTGAGAATTTGGGGGTCGGATAAGAGGCGTTGGCGTCGCACAGGGCATCGGCGCACGACTGCCGGATGGGGCGTGAACGCCTTATCCGGCCTACGGAGGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAAATTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCa < 2:204536/259‑1 (MQ=14) ccgggcgtcGGAGGGCGCGAGAACTTGTAGGTCTGATAAGACGCGGTAGCGTCGCCTCAGGCCTCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTAGCCGGCCTACTGATGGCGCGGGAATTTGTAGGCCTGATAGGACGCG‑TTAGCTTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCAc < 2:136946/266‑1 (MQ=11) ctgggacgACGGGTTAGGGTCGGATCGGGCATCTGCGCACGCCTGCCGGATGCGGCGTGAACGCCGTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCg‑g < 2:208642/268‑1 (MQ=255) gATAAGCCGCGTTAGCGTCGCGGCGGGCACGCGCGCAGGACTGCCGGATGGGGCGTGAACGCCGTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCG‑Gcc < 2:414053/275‑1 (MQ=14) ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGAAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGGCGCATCAGGCATCTGCGCACCACTGCCGGAGTCGGCGGAAACCCCTTTTCCG‑GCCTACgcagagc > 2:454672/1‑268 (MQ=14) aaagggtcgcgtcggggatggcgcACGGATGCCGGAGGCGGCGGGAACGCCTTATGCGGTCTACACTTCG‑GCCATAAACCGGAGGCCTGATAAGACGCGCAGAGCGGCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCG‑GCCTACGGATGgcg < 2:399916/254‑1 (MQ=11) tgagcagcaGGCAGCCGCGCAGGACTGCCGGATGCGGCGTGAACGCCTTAGCGGGCCTACGGATGGCGCGGGAATGTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCG‑GCCTACGGATGGCGCGGGa < 2:229680/272‑1 (MQ=255) gtgggagtaggcaggtggggaggACGGCCGGATGCGGCGTGAACGCCGTATCCGGCCGAGGGATGGCGAGGGAATGTGTAGGCCTGGTAAGAGGCG‑TTAGCGTCGGATCAGGCATCTGCGCACGGCTGCCGGATGGGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCG‑GCCTACGGATGGCGCGGGa < 2:469270/253‑1 (MQ=11) gCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGgc > 1:125823/1‑165 (MQ=38) cATCAGGCATTTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGAGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGACTTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCG‑GCCTACGGCTGGCGCGGGAAttt > 1:372811/1‑275 (MQ=255) cATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTCGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCG‑GCCTACGGATGGCGCGGGAAttt > 1:272610/1‑275 (MQ=255) cATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGGGCGCGAGAATTTGTCGGCCTGATAATACGCGTTACCGTCCCATCCGGCATCTGCGCCCGACTGCCGGATCCGGATCAAACCCCTTATCCC‑GCCCACGGCGGGCGCGGGaa > 2:496199/1‑272 (MQ=14) cAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCga < 2:125823/165‑1 (MQ=39) gCAGCTGCGCACGACTGCCGGATGCGGCGGAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCG‑GCCTACGGATGGCGCGGGAATTTGTAGGc < 1:133600/275‑1 (MQ=255) cATCTGAGAACGAGTGCCGGAGGCGGCGTGAACGCCTTATGCGGACTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCG‑GCCTACGGATGGCGCGGGAATTTGTAGGcc < 2:448781/275‑1 (MQ=255) cGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGTTAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCC‑GCCTACGGATTGCGCGGGATTGTGTAGGCCTGATTAgca > 1:342925/1‑272 (MQ=255) cGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGAAAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCACCAGGCACCTGCGCACGACTGCCGGAGGCGGCGTAAGCCCCTTACCCC‑GCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGAc > 2:226806/1‑274 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGCTGCGGCGCAAACGCCTTCACCG‑GCCTACGGATGCCGCGGGCATTCGTAAGCAGGTTAAGGCGCGGTa > 2:436634/1‑274 (MQ=14) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGCGGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTCGCGTCGCATCCGGCATCTGCGCACGACTGCCGGATGCGGCGTCAACCGCTT‑TCCGCGCCTCCGGACCGCGCGGGCATTTGTAGGCACGATACAACGCGtaa > 2:28917/1‑272 (MQ=11) ccTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTCTCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTAACCC‑GCCTTCTGATCGCTCGGGCATTTGGGACCCTGATAAGTCGCGCGAGCGCTCCCCTC‑GGCATCCGCACACgcacg > 2:46910/1‑271 (MQ=14) ggCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGGAAACGCCTTATCCG‑GCCTACGGATGGCGCGGGAATTTGTAGGCCTGTTAAGACGCGTTGACG‑GCGCCTCCGCCACCTCACCACGACCGCCGGAAGc > 2:336303/1‑274 (MQ=14) gcgcGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATCAGACGCGTTAGCGTCCCATCAGGCATCTGCGCACGAATGCCGGATGCGGCGTAAACGCCTTATCCT‑GCCTACGGATGGCGCGGGGATTTGTCGGGCGGATCAGACGCGTTAGCG‑CCGAACGAGGCACCTGCACACCACTGCCgtaac > 2:299942/1‑268 (MQ=14) | CTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCG‑GCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCG‑TCGCATCAGGCATCTGCACACGACTGCCGGATGC > NC_000913/374804‑375342 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |