| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,614,497 | C→T | 9.3% | intergenic (+236/+184) | yjjV → / ← yjjW | putative DNase/putative pyruvate formate lyase activating enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,614,497 | 0 | C | T | 9.3% | 115.9 / 2.8 | 50 | intergenic (+236/+184) | yjjV/yjjW | putative DNase/putative pyruvate formate lyase activating enzyme |
| Reads supporting (aligned to +/- strand): ref base C (27/16); new base T (2/2); total (30/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
TGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACGCG‑TCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCG > NC_000913/4614224‑4614760 | tggttaagaaccacatgacgtggcgtAACGGGCCGTAGGCCGGATAAGGCGTTCGCGCCGCATCAGGCAGTTGGCGCACAATGCCGGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTc < 2:81235/249‑1 (MQ=14) gtttACTACCACGCTTACGTTGTTTAACGTGCCGTAGCCGGGAGAAGGCCTTCACTCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCg < 1:456227/272‑1 (MQ=17) gCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCCTCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCTCACCCGGCAGTccg > 2:184371/1‑273 (MQ=255) cGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGGGTCTCATCCTGCCTACAAGCATGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCAGCCGGCAGTTGgcgccccatgcc > 2:364298/1‑267 (MQ=14) cGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGcc > 1:220857/1‑275 (MQ=255) tttAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCTTCGGCCGGATAAGGCGTTCCCGCCGCATTCGGCTGTCGGCGCATAATGCCTGATGCGCCg > 1:420473/1‑274 (MQ=255) tttAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCCTCCGGCCGTCGGCGCAAAATGCCTGATGCGACGc > 1:350240/1‑275 (MQ=255) gtgcgcgggcggggATAAGGCTTTCGTGCCGCATCTGGCCGTGCGGGCCAAGTGGCGGATGGGGCGCTTAACGCGTCTGATCATGGCTACAGGTTGGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGAACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCttaac < 2:267751/262‑4 (MQ=11) gTGCCGTAGGCCGGATAAGGCGTTCACGCCGCAACCGGCAGTTTGGGCACAATGCCTGATGCGACGCTTAACGCGTTTCATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCTGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGAAGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTc < 1:68007/274‑1 (MQ=255) ccGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGCTAAGGCGTTCACGCCGCATCCGGCAGGCGGCGCCTAAACCCTGATGCGACGCTTGTccggt > 2:291119/1‑271 (MQ=255) tAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCGGATGAGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAAGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGAACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCtt < 1:417232/275‑1 (MQ=255) gCCGGATAAGGCGTTCCCGCCGCATGCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATc < 2:313614/275‑1 (MQ=255) cACGCCGCATCCGGCAGTGGGCGCACAATGCCGGATGCGCCCGTTAACGCGTCTTATAATGCCGACAGGTTGGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGttt < 2:220857/274‑1 (MQ=255) gaGTTTGCGAACAATGCCGGGAGCGGCGCTTAACGCTGCTTATCATGCCTTCAGGTTTGGGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTGGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGGGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAgg < 2:350239/274‑1 (MQ=14) gggtgccacacaGTGACTGGTGCGCCGCTTACAGCGTCTTATCATGCGTACAGGTTTGTGCCGAACGGTAGGCCGGATAAGGCGTTCACGCCGCATCCTGCAGTTGGCGCACGATGCCGGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGc < 2:285707/267‑1 (MQ=14) cacaATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCCCATCCGGCAGTCGGCGCATAATGCCTGATGCGCCGCTTGTCGCGTCTTATCCGTCCTACAGTTTTGTTCCGCACCGTAGGCCGGATaa > 1:281482/1‑274 (MQ=255) cGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATTCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAACGCGTTACCGCCGCATcc > 1:428595/1‑275 (MQ=255) tAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCCGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATACGGCGTTCCCGCACCCTCCCGCCGTAGGCGCATAATTCATGATGCGACGCTTTTCGCCTCTTACCATGCCTACAGGCTTGTGCCGAACCGTAGGCCGGTACCGGCGTTCGcgccgcccccgcca > 2:463943/1‑267 (MQ=17) tCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCCGTCGGCGCATAATGCCTGACGCGACGCTTCTCGCCTCTTATCATGACCACCGGTTTGTGCCGAACCGTAGGCCGGAAAAGGCGTTcccccacgcacccgccgttggc > 2:250611/1‑253 (MQ=17) ttGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGc > 2:114394/1‑175 (MQ=255) tgtgCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAGTCGGCGCCTAATGCCTGATGCGACGCTTGTCGCGTCTTATCATTCCTCCCGGTTTGTGCCGAACCCTAGGCCGCACAACGCGTTCGCGCCGCCTCCGGCCGTTGGCGCCCCACGCCTGACG‑CGAcc > 2:290694/1‑273 (MQ=14) tgtgCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCTTCTTATCATGCCTACAGCTTTGTGCCCAACCGTAGGCTGGCTAAGGCGTTCCCGCCCCATCCGGCAGTCAGCGCACAATGCCTGATG‑CGACg > 1:275930/1‑274 (MQ=255) gCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGt < 1:114394/175‑1 (MQ=255) cGAACGGGAGGCTGGGGAAGGGGGTCCCGCCGCATCCGGCGGTGGGCGCACAATGCCGGGTGCGACGCTTTTAGCGTCTTTGCAGGCCTCAAAGTCGGTGCGGAACCGAAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCGGATGGGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGa < 2:428130/274‑1 (MQ=14) gCCGGGTAAGGCGGTCACGGCGCAGCCGGCAGTTGGCGCACAAGGCGTGATGGGACGCTTGGCGCGTCTTATCATGCCTACAGGTCTGGGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACGCG‑TCTTATc < 2:27533/275‑1 (MQ=14) aTAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGACACGGCCTTCGCGCCGCATCCGGCCGTTGGCGCCCCATGCCTGATC‑AGCCGCTTCACGGC‑TCTTATCAGGc > 2:111975/1‑274 (MQ=14) gCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTCTCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGCTAAGGAGTGCGCTCCGCATCCGGCCGTTGGCGCACCATGCCTGATG‑CGACGCTGGACGCG‑CCGTAtaccgcctgaa > 1:282798/1‑264 (MQ=14) gCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCCCATACGGCAGTCGGCGCATAATGACTTATGCCGACG‑TTAACGCGTTCTTA‑CAGGCCtcaa > 2:342904/1‑271 (MQ=14) cACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGAt > 1:399418/1‑194 (MQ=255) ccgcATCCGGCAGTGGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAgg < 2:399417/194‑1 (MQ=255) cgcATCCGTCAGTCGGCGCACAATGCATGAGGCGACGCTTGTCGCGTCTGATCATGCCTACAAGTCTGGGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATTCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACGCG‑TCTTATCAGGCCTACAAGTCTGTGcc < 1:330890/274‑1 (MQ=255) ttGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCCGTTGGCGAACAATGCCTGATG‑CGACGCTTGACGCG‑TCTTATCCGGCCTACAAGTCTGTGCCGAACCGTAGGcc > 1:115507/1‑274 (MQ=255) tGCCTGATGCTACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACGCG‑TCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCAt < 1:364299/274‑1 (MQ=255) tGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACGCG‑TCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCAt < 1:342905/274‑1 (MQ=255) gccttggtggccGCTTGTCGGGCCTAATCATGCCGACAAGTCGTGGCCGAACCGTGTGCCGGATAAGGCGTTCACGCGGCACCCGGCAGTGGGCGCAGAATGCCGGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGTCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACGCG‑TCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGt < 2:281482/264‑1 (MQ=17) gATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCCTGTCGCGTCTTATCATGCCTACAGGCTTGTGCCGAACCGTAGGCCGGATAAGGCGCTCGCGTCCCCTCCGTCATCTGGCCCCCAATGCCTGATG‑CGACGCTTGACGCG‑CCTTCTCAGGCCTACACGTCTATGCCGCACCGCACGCGGTAACCGGcgcgtccc > 2:254620/1‑267 (MQ=17) aTGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGTGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCCCCGCCTCCGGCCGTTGGCGCCCAACGCCTGACG‑CGACGCTTGCAGCC‑ACTTCTCAGGCCTACAAGTCTGTCCCGAACCGTAGGCCGTCACCGGCATCTCCCaa > 2:184687/1‑275 (MQ=17) aTGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACCCC‑TCTTCTCAGGCCTACAAGTCTGTGCCCAACCGGAGGCCGTATCCGGCATGTCGCaa > 2:3052/1‑275 (MQ=255) aCGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGGTTGTGCCGAACCGTAGGc > 2:264708/1‑150 (MQ=255) ttGTCGCGTCTTAGCATGCCTACAAGTCGGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACGCG‑TCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAgcgc < 1:291119/275‑1 (MQ=255) aTCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACGCG‑TCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCaa > 1:49238/1‑275 (MQ=255) gCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACGCG‑GCTTATCAGGCCTACAAGTCTGTGACGGACCGTAGGCCGTATCCGGCATGTCACACATAGAGCGCCGGCAATATCACCCCGCTCACCCCGCGCAc > 1:402110/1‑274 (MQ=255) aaCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGTCGCG‑Tc > 2:350046/1‑177 (MQ=39) aaCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACGCG‑Tc < 1:350047/177‑1 (MQ=255) tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACGCG‑TCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCCCAACTAGACCGCCGGAAATATCCACCGGCTCCCCCCCCCACCCTTTAAccc > 2:218311/1‑272 (MQ=17) gCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACGCG‑TCTTATCAGGCCTACAAGTCTGTGCCGAACCGGAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCCACCGGACCCACCCGCGCACCTTTAACGCATCa > 1:43122/1‑275 (MQ=255) ggtAAGGCGTTCACGCCGCAGCCGGCAGTCGGGCGATAGGGCCGGATGCGACGCTTGTCGCGTCTTATCAGGCCTACAGGTGTGTGCCGAACCGTAGGCCGGATAAGGCGTGCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACGCG‑TCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCa < 2:420472/273‑1 (MQ=21) gATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGAATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGCCGCG‑TCTTATCAGGCCTACCATTCTGTGCCGAACCGGAGGCCGTCTCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGAACATTTAACGCATACGCCa > 1:172711/1‑275 (MQ=18) aTAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACGCG‑TCTTATCCGGCCTACAAGTCTGGGCCGAACCGTAGGCCGTACCCGGCCTGTCCCAAATACAGCGCCGGAAATATCCACCGGCTCACCCCGCGCCCCTTTAACGCATCAGccc > 2:431454/1‑273 (MQ=255) ggCGTTCCGGCGGCTTCCGGCGTTGGGGGCATAATGCCGGATGTGACGCTGCGCGCGTCTTATCATGCCTACAGGTGTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACGCG‑TCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGc < 2:428594/275‑1 (MQ=21) tCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACGCG‑TCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCaa > 1:80859/1‑274 (MQ=255) aTCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACGCG‑TCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCCCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCg > 1:180327/1‑274 (MQ=255) | TGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATG‑CGACGCTTGACGCG‑TCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCG > NC_000913/4614224‑4614760 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |