Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	659,993	T→G	17.0%	N75T (AAC→ACC)	lipA ←	lipoate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	659,993	0	T	G	17.0%	92.0 / 2.5	47	N75T (AAC→ACC)	lipA	lipoate synthase
Reads supporting (aligned to +/- strand):  ref base T (10/26);  new base G (8/2);  total (19/28)
Fisher's exact test for biased strand distribution p-value = 7.62e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CGGGCTTTTTTCCCGAATGGCAGTAATGCAATCCGCAAAGTGCTGGGCACCGCCATCGCGCAGGTCATCACGGTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTC  >  NC_000913/659734‑660258                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                           cGGGCTTTTTTCCCGAATGGCAGTAATGCAATCCGCAAAGTGCTGGGCACCGCCATCGCGCAGGTCATCACGGTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGGGGGTGAATCATTCCGCCCGGGTCGGGCAGGGGGCtt                                                                                                                                                                                                                                                             >  2:108410/1‑275 (MQ=255)  gggCGTTGTTACCGAATGGCAGTAATCCAATCCGCAAAGTGCTGGGCACCGCCATCGCGCAGGTCATCACGGTCAACGGTGGTTATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCTTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTc                                                                                                                                                                                                                                                            <  1:96973/275‑1 (MQ=255)       ttattaccGAATGGCAGTAATGCAATCCGCAAAGTGCTGGGGACCGCCAGCGCGCATGTCATCACGGTCAACAGAGGTGAGAACCACATAACGCAGGGCCAGATCGGCAATGGGCTGCGCCAGTGTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑c                                                                                                                                                                                                                                                      <  1:282655/269‑1 (MQ=255)         cttCCTGAAGGGACGTAATGAAATCCGAAAAGTGCGGGGCACCGCCAGGGCGCAGGTCATCACGGGCAACGGAGGTGATAACCACATCACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑c                                                                                                                                                                                                                                                      <  2:89259/272‑1 (MQ=255)                  ggggagaaagtccaacagCAAAGTGCTGGGCACCGCCATCGGGAAGGTCATCACGGTCAACGGAGGTGATAACCACAGAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATg                                                                                                                                                                                                                                            <  2:441051/257‑1 (MQ=255)                                tCCGCAAAGTGCTGGGCACCGCCATCGGGCAGGTCATCACGGTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGATAGGGCAGGGGGCTTCATCG‑CAGAACGAATGCAGGCCAttttttcc                                                                                                                                                                                                                             >  1:116666/1‑272 (MQ=255)                                gccGCAAAGTGCTGGGCACCGCCATCGCGCAGGTCATCACCGTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGGTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTgcg                                                                                                                                                                                                                             <  1:108410/274‑1 (MQ=255)                                                   ccccagagcgcAGGTCATCACGGTCAACGGGAGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGGGCCAGTGTCACTGGTTTATTGGCATTAGGGGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGACTTTGATGcc                                                                                                                                                                                                           <  1:114324/267‑1 (MQ=255)                                                     ccATCGCGCAGGTCATCACGGTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTTATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTAACAAAAGCGCCGGGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCCGGGTCGGGCCGGAGGCTTCCTCC‑CAGAGAAAATGCAGGGCCACTTTGCGCATTTCGGCTTTGAGGCCCt                                                                                                                                                                                                         >  2:114324/1‑274 (MQ=255)                                                        tccggcAGGTCATCACGGTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGa                                                                                                                                                                                                      <  2:253291/270‑1 (MQ=255)                                                        tccggcAGGTCATCACGGTAAACGGAGGTGATAACCACATAACCCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGa                                                                                                                                                                                                      <  2:206286/270‑1 (MQ=255)                                                        tCGCGCAGGTCATCACGGTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGa                                                                                                                                                                                                      <  2:488369/274‑1 (MQ=255)                                                                          tCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGCACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTTCTGTGCCGTGGTTGAAGCATTCCGCCCGGGTAGGGCCGGAGGCCTCCTCGCCAGCCA‑AATGCAGGGCCATTTTGCGCCTTTGGGCTTTTCTTCCCTGGACACAGGTTGAGTCCCCTgg                                                                                                                                                                                   >  2:154488/1‑275 (MQ=255)                                                                                      aTAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGGTAGGGCAGGAGGCTTCCTCG‑CAGAAAGAATGCAGGCCATTTTTTCGCATTGCGGCTTTGCTGCCCGGGGTACGTGGAGAGTCCCCTTGAAACTTTAtttt                                                                                                                                                                       >  2:287000/1‑275 (MQ=255)                                                                                             caTAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CCGACAGAATGCCGGACCTTTTTTCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGCAGCTTGCtttttctccc                                                                                                                                                                 >  2:317174/1‑268 (MQ=255)                                                                                                     aGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTTCCGTGGTTGAAACATTCCGCCAGGGTAGGGCAAGAGGCTTCCTCC‑CAGACAGAAGGCAGGCCCTTTTTGCGCATTGGGGCTTTGATGCCCCTGGTACGTGTACAGGCCGCTGGAAGCTTGATTTTCCTCCATTCCGGCt                                                                                                                                                         >  2:396221/1‑274 (MQ=255)                                                                                                     aGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGAGCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGGTCAGGCCGGAGGCCTCCTCG‑CAGACCGCATGCAGGCCATTTTTGAGCCTTGCGGCTTTGTTGCCCTGGATCCGTGTCGCGTCCGCCGCGCGCTTGATTTTTATCCACTCCGGcc                                                                                                                                                         >  2:118085/1‑273 (MQ=255)                                                                                                        gcCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCCGGAGGCTTCCTCG‑CCGCCAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCACCGGATACGTGTAGAGTCCGCTGGCAGCTTGGTTTTCATCCCTTCCCGCTTgc                                                                                                                                                      >  2:25032/1‑274 (MQ=255)                                                                                                                    aTGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCCCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCCTTGCGGCTTTTGTTCCCTTGCTACGGTTAGGGTCCCGTGGGAGCTTGAGTTTAATCCATTCCCGGTTTCGCAGCAGGGCtt                                                                                                                                          >  2:476240/1‑274 (MQ=255)                                                                                                                                  tttCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCCGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTTCGCATTGCGGCTTTGATTCCCCGGGTACGTTTAGGGTCTGCTGGAAGCTTGATTTTCATCCATTCCGGTTTTCCCAGCCGGGCGTCGCGCTCTGTTCCCa                                                                                                                           >  2:95167/1‑275 (MQ=255)                                                                                                                                  tttCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGGTAGGGCAGGAGGCTTCCTCG‑CACACAGAATGCAGGCCATTTTTTCGCATTGCGGCTTTGATGCTCTGGATACGTGTAGAGTCCGCTGGAAGCTTGTTTTTTATCCACTCCGGCTTGCGCCGCGGGGGCTCGCGCCCTTTTTCCa                                                                                                                           >  2:300677/1‑275 (MQ=255)                                                                                                                                    tCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATACCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGGTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATTCAGGCCATTTTTTCGCATTGCGGCTTTGATGCCCTTGATACGGGTTGAGTCAGCCGGACGCTTAGTTTTTATCCATTCCCGCTTTCGGCGCAGGGCCGCGCCCTCTGTTggcccg                                                                                                                         >  2:255244/1‑270 (MQ=255)                                                                                                                                                          aGCTACCGGGCGACCGTGGGCAACATCACAGACCGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGcc                                                                                                    <  1:347476/274‑1 (MQ=255)                                                                                                                                                                   gCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCgg                                                                                          <  2:337754/275‑1 (MQ=255)                                                                                                                                                                         gTGGGCAACCTCACAGAACGGACAACGGCTGGTAGAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAAGGCAGGCCATTTTTTCGCATTGCGGCTTTTATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGTGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATcg                                                                                     <  1:95167/274‑1 (MQ=255)                                                                                                                                                                         gTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATcg                                                                                     <  2:330928/274‑1 (MQ=255)                                                                                                                                                                           gggCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATcgcg                                                                                   <  1:25032/274‑1 (MQ=255)                                                                                                                                                                           gggCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATcgcg                                                                                   <  1:300677/274‑1 (MQ=255)                                                                                                                                                                                 cATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGTCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGCGGCCTCCTCG‑CAGACAGAATGCAGGCCATTTTTGGGCATTGCGGCTTTGATGCCCTGGAACAGTGTAGAGTCCGCTGGAAACTTGATTTTTCTCACTTCAGGCTTTGGCAACAGGGGTTCGCGGCTTGTTGTCCCGTTTTTAACCCGGCTCACGGccccttttttctagacggcgggtttt                                                                            >  2:299595/1‑251 (MQ=255)                                                                                                                                                                                         aaCGGAGAACGGCGGGTACACAGAGGGCCGAGGATCATAGACGTTGCTGGGCCGTGGTTGAAGCATTCCGCCAGGGTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCGTTGATGCCCGGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACTTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACcgcg                                                                    <  2:187045/275‑1 (MQ=255)                                                                                                                                                                                              aCAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGGGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGAGCCGCAGGGCTTCGCGCTCTGTTGCCCCGCTTTTAACCGGGATCAGGGCCCTCTTAACGGCATCGCGGTATTTGACCACGCGTTcc                                                                >  1:399065/1‑274 (MQ=255)                                                                                                                                                                                               cAACGGGGGGTAAAAATAGCGCCGGGGATCATAAACGTTGCTGGGCCGTGGGTGAAGCGTTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAAGGCAGGCCATTTTTGCGCATTGCGGTTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCAt                                                              <  2:2712/275‑1 (MQ=255)                                                                                                                                                                                                   ggcggGTACAAATAGCGCGGAGGATCATAAACGTGGCGGTGCCGTGGTTGAAGAATTCCTCCAGGTTAGGGCATGAGGCTTCCTCG‑CAGACAGACTGCAGGCCATTTTTGCGCATTTCGGCTTTGATGCCCTGGATACGTGTATAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATcaca                                                          <  1:255244/275‑1 (MQ=255)                                                                                                                                                                                                           cAAATAGCGGCGAGGATCAGAAACGGTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGtt                                                   <  2:399064/274‑1 (MQ=255)                                                                                                                                                                                                                aGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCa                                             <  1:154488/275‑1 (MQ=255)                                                                                                                                                                                                                    ccGAGGATCATAAACGTTGCTGGGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAg                                          <  2:189043/274‑1 (MQ=255)                                                                                                                                                                                                                                     tGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCCTCGCGGTATTTAAACCCCAGTTCCATCAACATGGGTTTCATCATAGCGTGCGTGTTCACGTTGc                        >  1:94163/1‑275 (MQ=255)                                                                                                                                                                                                                                      gCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCCTCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCg                       >  1:59675/1‑275 (MQ=255)                                                                                                                                                                                                                                      gCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATc                                                             >  2:442293/1‑237 (MQ=255)                                                                                                                                                                                                                                       cTGGGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGGTTCCTCG‑CAGAAAGAATGCAGGCCATGTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCg                       <  2:37633/274‑1 (MQ=255)                                                                                                                                                                                                                                          tgCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACaa                                                         <  1:442294/237‑1 (MQ=255)                                                                                                                                                                                                                                           gcctgggTTGAAGCATTCCGCCAGGTTAGGGCAGGGGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTa                  <  2:94163/270‑1 (MQ=255)                                                                                                                                                                                                                                                     aaGCATTCCGCCAGGTTATGGCAGGAGGCTGCCTCG‑CAGACAGAATGCATGCCATTTTTGCGCATTGCGTCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGTGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAg        <  1:287000/275‑1 (MQ=255)                                                                                                                                                                                                                                                         aTTCCGCCAGGGTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCCTTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCAACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGt     <  1:396222/274‑1 (MQ=255)                                                                                                                                                                                                                                                           tCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGCTCCATAACAATGGGTTTACGCATAGCGTGCGTGTTCCAGTTGCGAAATACGAAGGAACGCGttta  >  1:446217/1‑274 (MQ=255)                                                                                                                                                                                                                                                           tCCGCAAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCGTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGttt   <  1:317174/274‑1 (MQ=255)                                                                                                                                                                                                                                                               ccAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGtt                                                   <  2:219053/222‑1 (MQ=255)                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                           CGGGCTTTTTTCCCGAATGGCAGTAATGCAATCCGCAAAGTGCTGGGCACCGCCATCGCGCAGGTCATCACGGTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCG‑CAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTC  >  NC_000913/659734‑660258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑