Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,240,873 G→A 7.2% P400L (CCG→CTG)  cvrA ← putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,240,8730GA 7.2% 161.2 / 3.9 56P400L (CCG→CTG) cvrAputative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (24/27);  new base A (2/2);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.60e-01

ACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGA  >  NC_000913/1240606‑1241140
                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                           
aCCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTcc                                                                                                                                                                                                                                                                      >  2:25410/1‑275 (MQ=255)
  ctgctgcgACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACACCACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGCAACAGTGCCGCAATACGCGTCTCTTTTAGCATATGCACATCGCGCAGTGCCGCGCCCACGCACCATTTAACGGCACTCAATTGATAACCAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCa                                                                                                                                                                                                                                                                    <  1:231031/270‑1 (MQ=255)
                    cAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCTTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACt                                                                                                                                                                                                                                                   <  1:179520/274‑1 (MQ=255)
                        ttACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGCTATCACGAAACAGTGCCGCAATACGCGTCTCTTTAGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTACCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGCTGAATATCCAGGCCAACGCGTGACACCGGACGTCCCAATGGCGGAACCACCACTTTGGc                                                                                                                                                                                                                                              >  1:350280/1‑275 (MQ=255)
                               gcgcCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTAGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACCGGCGGAACCCCCCCTTTGGCTTTTTTa                                                                                                                                                                                                                                       >  2:428870/1‑275 (MQ=255)
                                cgcCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGCAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAACTGATAAACAAACTGCCCCCACGGCTTTTCCGGATGAATATCCAGGCCCACGCGTGACACCGGACGTCCCACTGGCGGAACCACCCCCTTGGCTTTTTTAg                                                                                                                                                                                                                                      >  2:247079/1‑275 (MQ=255)
                                 gcCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGAAAAACAAACTGCCCCCACGGATTTTCCGGATGAATATCCAGGCCAACCCGTGAAACCGGACGTCCCACTGGCCGAACCACCACTCTGGCTTTTTTAgc                                                                                                                                                                                                                                     >  2:491068/1‑275 (MQ=255)
                                 gcCGGAAGATCGCGTGCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAg                                                                                                                                                                                                                                      <  2:197934/274‑1 (MQ=255)
                                      aaGATCGCGTTCCCGAGCAATTACACCCAACACATCGCCTTCGCGCAGTCGGGTGCTGCCTGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAgcc                                                                                                                                                                                                                                    <  1:223383/271‑1 (MQ=255)
                                          tCGCGTTCCCGTCCAATTCCACACACCCAGTCGCCTTCGCTCAGTCGGTGGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACATTGCCGTAATACACGTCTCTTTTGGCATATGCAAAGCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACga                                                                                                                                                                                                                            <  1:471764/275‑1 (MQ=255)
                                               ttCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTg                                                                                                                                                                                                                       <  1:25410/275‑1 (MQ=255)
                                                  ccGACCAATTACACGCAACACATCGCCTTGGCGAAGTCGGGTGCGGCCGGTAGGATGAAGCAACTGGGTATCACGAAGCAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATg                                                                                                                                                                                                                    <  2:392328/275‑1 (MQ=255)
                                                          ttACACACAACACATCGCCTTCGCGCATTCGGGTGCGGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTGTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGc                                                                                                                                                                                                            <  1:449089/275‑1 (MQ=255)
                                                              acacaacacaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAaga                                                                                                                                                                                                        <  1:426979/275‑1 (MQ=255)
                                                               cacaacacaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAagag                                                                                                                                                                                                       >  2:30340/1‑275 (MQ=255)
                                                                ccaacacaTCGCCTTCGCGACGTAGGGTGCTGCCGGTAGGATTAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGc                                                                                                                                                                                                      <  1:262539/274‑1 (MQ=255)
                                                                    cacaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAgccg                                                                                                                                                                                                                                   >  2:237667/1‑242 (MQ=255)
                                                                    cacaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAgccg                                                                                                                                                                                                                                   >  1:338875/1‑242 (MQ=255)
                                                                    cacaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAgccg                                                                                                                                                                                                                                   <  1:237667/242‑1 (MQ=255)
                                                                    cacaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCCTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCTTGCAAGAGCAggg                                                                                                                                                                                                  >  1:359738/1‑273 (MQ=255)
                                                                    cacaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGAGTGACACCGGACGTCCCACTGGCGGAACCACCCCTTTGGCTTTTTTAGCCGCCCACGAGCGTGATGTTCCCTGCAAGAGCAGtt                                                                                                                                                                                                  >  1:294438/1‑274 (MQ=255)
                                                                       aTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCAGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGaaa                                                                                                                                                                                               >  2:275121/1‑275 (MQ=255)
                                                                        tCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCa                                                                                                                                                                                                                               <  2:338874/242‑1 (MQ=255)
                                                                        tCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTAGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAAc                                                                                                                                                                                              <  1:428871/275‑1 (MQ=255)
                                                                           gcTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAg                                                                                                                                                                                           <  1:481695/274‑1 (MQ=255)
                                                                           gcTTCGCGCAGGCGGGTGCTGCCGGTAGGATGAAGAGACTGGTTATCACGAAACAGTGCCGCAATACGCGTCGCTTTAGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAg                                                                                                                                                                                           <  2:350279/274‑1 (MQ=255)
                                                                               cgcgCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTCGCCGCCCCAGCGAGTGATGTTCCCTGCAAGGGCAGGTAAAACAGAAcc                                                                                                                                                                                       >  2:123285/1‑275 (MQ=255)
                                                                                gcgcAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCCCCCACGCACCATTTATCGGCACTCCATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGGGACACCGGACGTCCCCCCAGCGGAACCACCACGTTGGTCTTTTTACCCGCCCACGAGAGTGACGTTCCCGGCTAGCCCAGTGAAACCACAAccc                                                                                                                                                                                      >  1:208301/1‑274 (MQ=255)
                                                                                     gTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCAGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAaag                                                                                                                                                                                 <  1:275121/275‑1 (MQ=255)
                                                                                              tgcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTTCTAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCAACGCGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGACCCACAAAGACGGCGACa                                                                                                                                                                        >  1:357511/1‑275 (MQ=255)
                                                                                              tgcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACa                                                                                                                                                                        >  1:311844/1‑275 (MQ=255)
                                                                                                                aCTGGTTATCACGAACCAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGGGCCGCGACCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCa                                                                                                                                                      <  1:158832/275‑1 (MQ=255)
                                                                                                                                         aatcagcgTCTCTTTTGCCTACTGCAAATCGCGCAGGGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCCCGGATTTGCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGa                                                                                                                             <  2:426929/270‑1 (MQ=255)
                                                                                                                                          aTACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGGCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGaa                                                                                                                            <  2:311844/275‑1 (MQ=255)
                                                                                                                                                  ctctTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGAAAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGTCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACgg                                                                                                                       <  1:403299/272‑1 (MQ=255)
                                                                                                                                                   tctTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTCGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTCAAGAACAGCCGTGCATTCTCCAGCCCCGCCATCATCGGGAAACCGGCCa                                                                                                                    >  2:323763/1‑274 (MQ=255)
                                                                                                                                                                  aaaTCGCGGCGTGCCGCCGCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTGTTAGCCGCCCGCGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCAccgc                                                                                                    <  2:325290/275‑1 (MQ=255)
                                                                                                                                                                   aaacgcgCAGGGCCGCGCCCACGCACCATGTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGAGTTTCCGGATGAATAGCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCAccgcg                                                                                                   <  2:359737/272‑1 (MQ=255)
                                                                                                                                                                   aaTCGCGCAGTGCCGCCCCCACGCACCATTTAACGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCAccgcg                                                                                                   <  1:415437/275‑1 (MQ=255)
                                                                                                                                                                    aTCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAACTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCAACGAGAGTGATGTTCCCTGCAAGAGCAGGGAAACCAGAACCCCAAAGAAGGCGACATTAAAGAAAAGACGTGCATTCCCCAGCCCCGCCATCATCGGGAAACCGGCCAGGATGATTGGCAccgcc                                                                                                   >  2:318114/1‑273 (MQ=255)
                                                                                                                                                                                  cccccACGCACCATTTATCCGCACTCAATTGATAAACGAACTGCTCGCACGGATTTTCCGGATGAAGATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTAcc                                                                                    <  1:236876/273‑1 (MQ=255)
                                                                                                                                                                                    cccACGCACAATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCa                                                                                  <  1:123285/275‑1 (MQ=255)
                                                                                                                                                                                     ccACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAg                                                                                 <  1:30340/275‑1 (MQ=255)
                                                                                                                                                                                             cATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATaaa                                                                         >  2:409542/1‑275 (MQ=255)
                                                                                                                                                                                               tttATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGACCCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCCTCGGGAACACGGACAGGATGATTGGCACCGCGCCGCCTAATCCTCCCCAGCTGATAAaca                                                                       >  2:410935/1‑275 (MQ=255)
                                                                                                                                                                                                 tATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCCCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTCATCCTCCCCAGCTGCTAAACAcg                                                                     >  2:350144/1‑275 (MQ=255)
                                                                                                                                                                                                         cTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCAGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACg                                                                                                                                                          >  2:329246/1‑182 (MQ=255)
                                                                                                                                                                                                             aTTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCAGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCa                                                                                                                                                      <  1:329246/182‑1 (MQ=255)
                                                                                                                                                                                                                      caaacTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACcg                                                >  1:130488/1‑275 (MQ=255)
                                                                                                                                                                                                                      caaacTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCCCGTAATCCTACCCAGCTGATAAACACGCGCTCCCGCCGAGTGCAACcg                                                >  2:75440/1‑275 (MQ=255)
                                                                                                                                                                                                                                ccACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCAAGGAGGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATCAACACGCGCTCACGCAGATTGAACCCCggggagggga                                      >  2:153692/1‑275 (MQ=255)
                                                                                                                                                                                                                                                          ggCCAACGGCTGACGCCTGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTGTAGCCGCCCACGGGAGTGATGTTCCCTGGAAGGGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCGCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGa            <  2:348022/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                cgcgTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCt                                                                 >  1:496010/1‑216 (MQ=255)
                                                                                                                                                                                                                                                                 gcgTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCga     <  1:409543/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                    tGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACg                                                             <  2:496009/216‑1 (MQ=255)
                                                                                                                                                                                                                                                                    tGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCgaaga  >  2:356445/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                           
ACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCTCACGCAGATTGAAACCGCGGAAGGGGAGCAATCCAGCAAATACCGAAAGGGGACGGGCGAAGA  >  NC_000913/1240606‑1241140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: