Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,466,711 T→C 9.8% pseudogene (1320/2513 nt) ydbA → pseudogene, autotransporter homolog, interrupted by IS2 and IS30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,466,7110TC 9.8% 158.6 / 2.2 55pseudogene (1320/2513 nt)ydbApseudogene, autotransporter homolog, interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base T (27/22);  new base C (4/2);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGG‑CACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATT  >  NC_000913/1466439‑1466988
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                       
atgaaGGGCGGCGACCGTGATGTCAGGGGCGGCGGGCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGa                                                                                                                                                                                                                                                                         <  2:230993/285‑1 (MQ=255)
                                                   aCCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACGGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGAATGTTAGAGGCGGCGGTCAC‑GGTATTg                                                                                                                                                                                                                     >  2:24875/1‑289 (MQ=255)
                                                   aCCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCa                                                                                                                                                                                                                              >  2:344098/1‑281 (MQ=255)
                                                   aCCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCa                                                                                                                                                                                                                              <  1:344098/281‑1 (MQ=255)
                                                                 aaCGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCCGTTGTCAACAACGAAGGCGCTGTCGCTTACACAAAC‑CTGGCGCTCCCGACGCCGACTAACGGTGATGACGCCACGGCAACCAACAACGGCAAAACCACCGTTGC‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGCTTCAGGCCGGCTGTCTGGATGTAAGCGGCGGCGGTCAC‑GGTATTGGTCTCCCCGGcca                                                                                                                                                                                                        >  1:369162/1‑286 (MQ=17)
                                                                        gATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGa                                                                                                                                                                                                        >  2:383951/1‑281 (MQ=255)
                                                                        gATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCAGCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGa                                                                                                                                                                                                        <  1:383951/281‑1 (MQ=255)
                                                                         gcaggttcagaagtgtaaaaagaagcGATCCGGAGTCCATCGGGATCCAGGTGGCCGGCGACCAGGAGGTTGTAAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCaa                                                                                                                                                                                                <  1:220483/263‑1 (MQ=17)
                                                                                gggCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGAt                                                                                                                                                                                        >  2:116351/1‑289 (MQ=255)
                                                                                  gCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGCTTCAGGACGGCGACCTGGATGTCAGCGGCGGCAGTCAC‑GGGATTGATATAACAGGCGACAGCGCAACGGGGGAGaa                                                                                                                                                                                      >  1:421878/1‑289 (MQ=255)
                                                                                  gCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATa                                                                                                                                                                                       >  2:159775/1‑288 (MQ=255)
                                                                                  gCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCGGTCGTCAACAACGAAGGCGAGAGCACCATCACCAACGGTGGCAT‑CGGCATCCAGAGTAGCGGAGGTGACGCTACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGACAAACATTCCACCCGCACGGCATTTTCCGGCAACAACGGGAAGGGCATTCACGACTGCCATCTGGCTGTCAGACGCGGCAGTCAC‑GGTAGTGCCATCACCGGcaccagtcacactgtcgataa                                                                                                                                                                                      >  1:144228/1‑269 (MQ=2)
                                                                                  agggtcagattggtataagaggcagttCCATCGGGATCCAGGTGGACGGCGACCTGGCGGTTTGTAACAACGAAGGCGGGGGCGCCATGACCAACGGTGGCAC‑CGGCACCCAGATTAATGGGGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATaa                                                                                                                                                                                      <  1:473078/263‑1 (MQ=1)
                                                                                        tGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAggg                                                                                                                                                                                >  2:81937/1‑289 (MQ=255)
                                                                                                 ccGAGCCGGCGTCAAGCGGTACCCAGGTTGACGGCGACAGGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGAGATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGAc                                                                                                                                                                       <  1:159775/289‑1 (MQ=255)
                                                                                                       acagggacATCGATATCCAGAGCGACGGTGACCAGGCCGTCGTTAACAACGAAGGCGGGAGCACAATCACCAACGGTGGCAC‑CGGAACCCAGATTAACGGGGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCa                                                                                                                                                                  <  1:171999/281‑1 (MQ=11)
                                                                                                              cATAGGTACACAGGTTGACGGGTACCAGGCGGTGGTTAACGACGAAGGCGAGGGCGCCATCAGCAACGGTGACAC‑CGTCACGCAGGTTAACGGTGATGACGGCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGGTAG‑GCTGTTGATACAGCCGGCTACAGGGGAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCg                                                                                                                                                          <  1:116351/289‑1 (MQ=17)
                                                                                                               aTCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATaa                                                                                                                                                                                      >  2:220483/1‑260 (MQ=255)
                                                                                                                    tagcaaGGTGGACGGCGACAAGGCAGTTGTTAACAACGAAGCCGAGAGAGCGACCACCAGCGGTGTCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTGGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGAAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGTCGGCGAC‑GGGATTGGTAGCCCCGGCCACAGGGCTACAATAGACAACAAGGGCACCATGACCGTCACCGATCCGGAGTc                                                                                                                                                     <  1:81937/283‑1 (MQ=14)
                                                                                                                    tATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCa                                                                                                                                                                                                                              >  2:157608/1‑216 (MQ=255)
                                                                                                                    tATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCa                                                                                                                                                                                                                              <  1:157608/216‑1 (MQ=255)
                                                                                                                    tATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCa                                                                                                                                                                                                                              <  2:334193/216‑1 (MQ=255)
                                                                                                                           gTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGGCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGCCCACCGGTa                                                                                                                                             >  2:72455/1‑289 (MQ=255)
                                                                                                                              gACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCACCGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATcc                                                                                                                                          >  1:213437/1‑289 (MQ=255)
                                                                                                                              gACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAATAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGAAAATCGCGGGCAATAACGGGATGGTAAATCAGGACGGCAGTCTGACTGTCAGCCGCTG‑AGACACATGTGATGCTGCCGACGGCGACAGAGGAATGGATGATAACAACGGCACCATGACCGCCACCGAACCGGCGCCCATCGGTATcc                                                                                                                                          >  1:144805/1‑289 (MQ=14)
                                                                                                                              gACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑g                                                                                                                                                                                                                           >  1:243656/1‑208 (MQ=255)
                                                                                                                              gACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑g                                                                                                                                                                                                                           <  2:243656/208‑1 (MQ=255)
                                                                                                                              gACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCACCGGTATcc                                                                                                                                          >  2:330468/1‑289 (MQ=255)
                                                                                                                              gACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGACGACGCCACCGCAAACAACAACGGCAAAACCACCGTTGA‑TGGAAAGGATTCCACCGGTACGGAAATCGCGGGCAATAACGGCAAGGTGAATCAGGACGGTGACCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGAGAACACTGGTGACAGCGCAACGGTTGATAACAAGTGCCTCATGCCCGTCACAGGTCCCGAATCCATCCGTacc                                                                                                                                           >  1:92822/1‑286 (MQ=14)
                                                                                                                                                        cGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGATGGTGATTCAGGACGGCGACCTGGATGTTAGCGGCGGCGGGCAC‑GGTATTGATATCACCGGTGACAGCGCAACGGTGGATAACAAGGGCACCAAGACCGTCACCGATCCGGAGTCCTGCGGGAACCAGAAGAACGGCGACACGGACATCGTc                                                                                                                >  1:7632/1‑289 (MQ=14)
                                                                                                                                                           aGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCaa                                                                                                              <  2:369162/288‑1 (MQ=255)
                                                                                                                                                               gaaggcaccATAACCAACGGTGTCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGCGTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGAGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACg                                                                                                         <  1:330468/282‑1 (MQ=255)
                                                                                                                                                                        aTCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCgaga                                                                                                <  2:144228/289‑1 (MQ=255)
                                                                                                                                                                        aTCACCAACGGTGGAAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCgaga                                                                                                <  2:421878/289‑1 (MQ=255)
                                                                                                                                                                         tCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGagag                                                                                               <  2:213437/289‑1 (MQ=255)
                                                                                                                                                                                 ggTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCa                                                                                       <  2:441950/289‑1 (MQ=255)
                                                                                                                                                                                   tGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATCGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGTTGTCCGCGTCGGCGGCCAA‑CGTATTGATCTTACCGGCGACAGCGCAACGGTGGATAACAAGGGCCCCAAGACCGTCACCGATCCGGCGTCCATCGGTATACAGATTCACCGCGCACAGGCACACGTAAACAACCAAgggacgagaacactcacc                                                                                     >  1:150730/1‑273 (MQ=14)
                                                                                                                                                                                     gCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCTATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCaa                                                                                   >  2:408288/1‑289 (MQ=255)
                                                                                                                                                                                     gCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCgaga                                                                                                >  2:220585/1‑276 (MQ=255)
                                                                                                                                                                                     gCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCgaga                                                                                                <  1:220585/276‑1 (MQ=255)
                                                                                                                                                                                     gCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGAAAATCGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGACCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCCACGGTTGACAACAAGGGCACCATGACCGTCCCCGATACTGGGTCGATTGGTATCCAGATTACCGGCGACCAGGCCATCGTCAATCACTAAGGcatgcacattgacccca                                                                                    >  1:428366/1‑272 (MQ=14)
                                                                                                                                                                                      cAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGCCAC‑GGTATTGATATCACCGGCGACATCGCCACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCATGCCATCGTCAATAACGAAGGCGAGAGCAATATCACCAAt                                                                                  >  2:183271/1‑289 (MQ=255)
                                                                                                                                                                                                     ttAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGcggcaccgg‑ca                                                                    <  2:389003/288‑1 (MQ=255)
                                                                                                                                                                                                      tAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATACCAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGCTTGACGGCGACCCGGCAATCGTCAATAACGAGTGCGAGAGCACTACCACAAATGGcgccatagg‑cac                                                                   >  1:149847/1‑288 (MQ=17)
                                                                                                                                                                                                              atgaCGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGcggcaccg                                                                        >  1:258230/1‑276 (MQ=255)
                                                                                                                                                                                                              atgaCGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGcggcaccg                                                                        <  2:258230/276‑1 (MQ=255)
                                                                                                                                                                                                              atgaCGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACAAGGCCAGCGTCAATAACGAAGGAGAGCGCACTAAAACCAACGGCGGTACCGGACAC‑CAGATCaa                                                          >  1:231989/1‑289 (MQ=17)
                                                                                                                                                                                                                                    cGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGCCAATACCGAAGGCGAGAGCACTATCACAAATGGCGGCACGGG‑CACTCAGATCAACGGTAACGACGCCACGGCGAAt                                    >  2:230527/1‑289 (MQ=255)
                                                                                                                                                                                                                                        aaaaCC‑CCGGTGACCGGCAAGGATGCCACAGGTGCTGCAATCGAGAGGAATAACGGGAAAGGTATTAAGGACGGCGATCTGGATGTAAGCGGCGGCGTTCAC‑GTTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCCGATTGACGGCGACCAGGCGGTCGTCAATAACGAATGCGAGAGCACTATCACCAACGGCGGCACCGG‑CACTCAGATCAACGGTAACGACGCCACCGCGAATAACa                                <  1:150357/289‑1 (MQ=14)
                                                                                                                                                                                                                                         aaaCCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGG‑CACTCAGATCAACGGTAACGACGCCACCGCGAATAACAg                               >  2:161366/1‑289 (MQ=255)
                                                                                                                                                                                                                                            ccaccGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGG‑CACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTgg                            <  2:231989/289‑1 (MQ=255)
                                                                                                                                                                                                                                                        ggCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGG‑CACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAAcc                     >  2:14022/1‑285 (MQ=255)
                                                                                                                                                                                                                                                                  ccACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGG‑CACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGt                <  2:301558/280‑1 (MQ=255)
                                                                                                                                                                                                                                                                  ccACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGG‑CACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGt                >  1:301558/1‑280 (MQ=255)
                                                                                                                                                                                                                                                                        gTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGG‑CACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGAtt  >  2:432019/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                       
ATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCAC‑CGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGA‑CGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAC‑GGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGG‑CACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATT  >  NC_000913/1466439‑1466988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑