Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,677,913 T→C intergenic (‑10/‑514) pntA ← / → ydgH pyridine nucleotide transhydrogenase, alpha subunit/DUF1471 family periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,677,9130TC98.6% 215.0 / ‑4.5 72intergenic (‑10/‑514)pntA/ydgHpyridine nucleotide transhydrogenase, alpha subunit/DUF1471 family periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (31/40);  total (31/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.85e-01

CAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGA  >  NC_000913/1677778‑1678046
                                                                                                                                       |                                                                                                                                     
cAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCg                                                                                                                                >  2:1180870/1‑143 (MQ=255)
 aGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCgg                                                                                                                               <  1:237382/143‑1 (MQ=255)
    tGACCCGCGCCGCTCTCTACCGCGGCGGTAAATCCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGttt                                                                                                                            <  1:1187211/143‑1 (MQ=255)
       cccGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTAt                                                                                                                         <  2:813848/143‑1 (MQ=255)
        ccGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTAtg                                                                                                                        >  2:122028/1‑142 (MQ=255)
          gtgccgccCTTTAGCTCGGCGGTAAATCCCCGTTTCAGGTGCTGTTCCACTGTTTTTGTCGTTGCTGCAACACTGGTTTCATTGGTTAATCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATtga                                                                                                                      <  1:1239692/141‑1 (MQ=255)
          gcgcCGCTCTCTCCCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTCTTGGCGTTGCGGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATtga                                                                                                                      <  1:600118/143‑1 (MQ=255)
           cgccgcGCTCTCCCGCGACGGTAAAACCCGGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGTTGCAACACGGGTTTCATTGGTTACCCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATtgat                                                                                                                     <  1:835976/143‑1 (MQ=255)
             ccgcTCCCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGCTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATtgatga                                                                                                                   <  2:262342/143‑1 (MQ=255)
              cgcTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTgatgat                                                                                                                  >  1:792746/1‑143 (MQ=255)
               gcTGTCTACCGCGACGGTAGAACCCAGTGTCAGCAGCGGTTCCACTGTTTTTCGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGGTTTATTGatgatg                                                                                                                 <  1:272267/143‑1 (MQ=255)
               gcTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGatgatg                                                                                                                 <  1:855261/143‑1 (MQ=255)
                ctctctACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATGCGCATGATATTCCCCTCCATCGGTTTTATTGATGATgg                                                                                                                >  1:491879/1‑143 (MQ=255)
                   tctACCGCGACGGTAAAACCCAGTTTTATCTGCTGTTCCTCTGTTTTTGGCGTTGCTGCAACACGGGTGTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGttt                                                                                                             <  2:421820/143‑1 (MQ=255)
                     caaggcgACTGTACAACCCAGTCTCTGACGCTCTTCTATTGTCTATGGCGTTGTTGCAACTGGCGCCTCCTTGGTTAAGCTTTTTTTTGGTATGCTAATTCGCTTTAATTTCTCCTTCTTTGTTTTTATTGATGATGGTTTGc                                                                                                           <  1:344756/139‑1 (MQ=255)
                       ccGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCt                                                                                                         >  2:471004/1‑143 (MQ=255)
                           gACGGTAAAGCCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTgtgt                                                                                                     <  1:1253163/143‑1 (MQ=255)
                           gACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTgtgt                                                                                                     <  2:405052/143‑1 (MQ=255)
                              ggTAAACCCCAGTTCCAGCAGCTGTTCCACTGTTGCTGGCGTCGCTGCAACACTGGTTTCAGTGGTTCACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTTCATCGGTTTTAGTGATGATGGTTTGCCTGTGTCAg                                                                                                  <  1:990897/143‑1 (MQ=255)
                               gTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCTgg                                                                                                 >  1:793189/1‑143 (MQ=255)
                               gTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCGCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGGTTGCCTGTGTCAgg                                                                                                 >  1:1175188/1‑143 (MQ=255)
                               gTAAAACCCAGTTGCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAgg                                                                                                 <  1:146283/143‑1 (MQ=255)
                                  acaCCCAGTTTCAGCAGCTGTTCCGCTGTTTTTGGCGTTGCTGCAACACGGGTTGCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGc                                                                                              <  1:648898/141‑1 (MQ=255)
                                   aaCCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGcc                                                                                             >  2:77497/1‑143 (MQ=255)
                                    aCCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTGTTATTGATGATGGTTTGCCTGTGTCGGGAGCca                                                                                            >  2:280576/1‑143 (MQ=255)
                                      ccAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCcaca                                                                                          >  2:216893/1‑143 (MQ=255)
                                       cAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCcacac                                                                                         <  1:336030/143‑1 (MQ=255)
                                          tttCAGCAGCTGTTCCCCTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAg                                                                                      <  1:163630/143‑1 (MQ=255)
                                          tttCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCACGATATTCCCCTCCATCGGTGTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAg                                                                                      >  1:668633/1‑143 (MQ=255)
                                              agcaggTGTTCCACTGTTTTTCGGGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCGAATTCGCATGATATTCCCCTCCATCGGTTTTATCTATGATGGTTTGCCTGTGTCAGGAGCCACACAAgctgc                                                                                  <  1:607027/143‑1 (MQ=255)
                                              agcagcTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAgctgc                                                                                  >  1:759541/1‑143 (MQ=255)
                                                   cTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTAGGCCAATTCGCATGAGATTCCCCTCCATCGGTTGTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATg                                                                             <  2:896709/143‑1 (MQ=255)
                                                    tGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATCGGTTAACCGTTCTCTTGGTATGTCAATTCGCATGATATTCCCCTCCATCGGCTTTATTGATGATGGTTTGCCTGTGTCAGTCGGCCCACGAGCTGCTCATGt                                                                            >  1:1063632/1‑143 (MQ=255)
                                                     gTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGTTTTGCCTATGTCAGCAGCCACACAAGCTGCTCATGTa                                                                           >  1:1049434/1‑143 (MQ=255)
                                                        ccACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGa                                                                        <  2:884869/143‑1 (MQ=255)
                                                        ccACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGa                                                                        <  1:1078497/143‑1 (MQ=255)
                                                        ccACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTGTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGa                                                                        <  2:1288803/143‑1 (MQ=255)
                                                          aCTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGc                                                                      >  2:6843/1‑143 (MQ=255)
                                                              tttttGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTGTGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTaaa                                                                  >  2:1349261/1‑143 (MQ=255)
                                                              tttttGGCGTTGCTGCAACACGGGTGTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTaaa                                                                  <  1:16641/143‑1 (MQ=255)
                                                               ttttGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAAt                                                                 <  1:673647/143‑1 (MQ=255)
                                                                tttGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATg                                                                >  2:820233/1‑143 (MQ=255)
                                                                tttGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATg                                                                <  2:1020305/143‑1 (MQ=255)
                                                                 ttGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGt                                                               <  2:1028434/143‑1 (MQ=255)
                                                                  tGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTACCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGtt                                                              >  1:451629/1‑143 (MQ=255)
                                                                    gCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGACATTCCCCTCTATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCACTTACGAGCTAACTCTTAc                                                            >  1:622598/1‑143 (MQ=255)
                                                                     cGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACt                                                           <  1:1223334/143‑1 (MQ=255)
                                                                           tgcAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTa                                                     <  1:132102/143‑1 (MQ=255)
                                                                             taaCACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTaaa                                                   <  2:1008053/142‑1 (MQ=255)
                                                                             cAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTaaa                                                   <  2:1034596/143‑1 (MQ=255)
                                                                              aaCACGGGTTTCATTGGTTACCCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTaaaa                                                  <  2:17009/143‑1 (MQ=255)
                                                                                 acGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAaaataa                                               >  1:813164/1‑143 (MQ=255)
                                                                                      tttCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAg                                          >  2:533523/1‑143 (MQ=255)
                                                                                          aTTGGTTAACCGTTCTTTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAAc                                      <  2:664559/143‑1 (MQ=255)
                                                                                          aTTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAAc                                      >  2:1017171/1‑143 (MQ=255)
                                                                                              gtgagccgtgttgttGGTATGTCAATTCGCATGAGATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAAc                                  <  1:755957/130‑1 (MQ=255)
                                                                                              gTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGCTGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTGATGTACGAGCTAAATCTTACTCCGGCAACATAAATTACTAACAGAc                                  >  1:1334895/1‑143 (MQ=255)
                                                                                              gTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTCTTAGTGATGATGGTTTTCCTGTGGCAGGAGCAACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTACAAAAACATTAGTaaacaag                                  >  1:717958/1‑138 (MQ=255)
                                                                                               ttAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACg                                 >  2:409959/1‑143 (MQ=255)
                                                                                                tAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGc                                <  2:759541/143‑1 (MQ=255)
                                                                                                    cGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGTAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAATAACAAACGCCAta                            >  1:1194752/1‑143 (MQ=255)
                                                                                                     gTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCgat                           >  1:455629/1‑143 (MQ=255)
                                                                                                        ctctTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGc                                                                      <  1:548517/97‑1 (MQ=255)
                                                                                                        ctctTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGc                                                                      >  2:548517/1‑97 (MQ=255)
                                                                                                          ctTGGTATGCCAATTCGCATGATATTACCCTCCATCGGTTTTATTGCTGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGCGCTAAATGTTACTCCGTTACAATAAACTAGTAACCAAACCCCTATAACgg                      >  1:120109/1‑142 (MQ=255)
                                                                                                           ttagTATCCCAAGTCGCATGACATTCCCCTCCATCGGTTGTATTGATAATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTa                     <  2:1303133/140‑1 (MQ=255)
                                                                                                                aTGCCAATTCGCATGATATTCCCCTCCTTCGGTTTTATTGATGATGGGTTGCCTGTGTCAGGATCCACCCAAGCTGCACGTGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGaa                <  1:657677/143‑1 (MQ=255)
                                                                                                                  gCCAATTCGCGTGATATTCCCCTCCAGCGGTTTTATTGATGATGGTGTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGaaaa              <  1:180934/143‑1 (MQ=255)
                                                                                                                   ccgaTTCGCATGATTTTCCCCTCCATCGGTTTTATTGGTGATGGTTTGGCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAAt             <  1:873479/140‑1 (MQ=255)
                                                                                                                          gCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCt      <  1:820233/143‑1 (MQ=255)
                                                                                                                           cATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCtg     >  2:819608/1‑143 (MQ=255)
                                                                                                                              gATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGa  >  2:609627/1‑143 (MQ=255)
                                                                                                                                       |                                                                                                                                     
CAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGA  >  NC_000913/1677778‑1678046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: