Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,542 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5420CA97.7% 276.4 / NA 87P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (43/42);  minor base G (1/1);  total (44/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184403‑4184680
                                                                                                                                            |                                                                                                                                          
cTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACACGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCt                                                                                                                                         >  1:1250602/1‑143 (MQ=255)
  ggCTGTTAACCGCCGGATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCCCTCGAATATATGCCTTACGCTGAAAACGGTACGCCGGTAGACGTCGTACTGAACCAGCTgg                                                                                                                                       <  2:443099/143‑1 (MQ=255)
  ggCGGTTAAACGCCGGATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGGCATTTGTAAGACCAACCCGATCGAAGATATGCCGTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                       <  1:516324/143‑1 (MQ=255)
  ggCGGTGAACCGCCGTATCCAGCCTGGTGACATGCTGGCAGCTCGTCACGGTAACAAGGGTCTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGACGAGCACCATATGGCTGTAGACATCCTACTGAACCAGCTgg                                                                                                                                       <  1:609694/143‑1 (MQ=255)
   gCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTggg                                                                                                                                      >  2:1350617/1‑143 (MQ=255)
     ggTTAAACGCCGTGTCCAGCCTGGTGACCAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCg                                                                                                                                    <  1:1254024/143‑1 (MQ=255)
     ggTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGGCATCGTGCTGAAGCGGCTGGGCg                                                                                                                                    >  2:1263385/1‑143 (MQ=255)
      gTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGt                                                                                                                                   >  1:256068/1‑143 (MQ=255)
        tAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAc                                                                                                                                 <  2:1296263/143‑1 (MQ=255)
         taaCGCCGTTTCGTGCCTGTTGCCAACTTTGTAGGTCGTCACGGTAACTAGGGTGTAATTTCTAAGATTAACCCGATCGTAGTTATGCCTTACGATGACATCGGCACGCCGCTAGTCTTCGTATTGAACCAGCTGGGTGTAcc                                                                                                                                <  1:50461/142‑1 (MQ=255)
         aaaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGACGATATGCCTTGCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAcc                                                                                                                                >  2:686656/1‑143 (MQ=255)
           ccGCCGTATCCAGCCGGGTGACAAGATGGCAGGTCGTCACGGTCACGCGGGTGTAATTTCTAAGATGAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGt                                                                                                                              <  2:568693/142‑1 (MQ=255)
           aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTCGACATCGTACTGAACCAGCTGGGCGTACCgc                                                                                                                              >  2:784332/1‑142 (MQ=255)
           aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGt                                                                                                                              >  1:202790/1‑143 (MQ=255)
             gCCGTATCCAGCCTGGTGACAAGATGGCAGGTCTTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGCGGACATCGCACTGAGCGAGCGGGGCGTACCGtct                                                                                                                            >  2:1354119/1‑143 (MQ=255)
             gCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTCCTGAACCAGCTGGGCGTACCGtct                                                                                                                            >  2:608642/1‑143 (MQ=255)
                gTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGt                                                                                                                         <  1:632541/143‑1 (MQ=255)
                gTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGt                                                                                                                         <  2:452033/143‑1 (MQ=255)
                gTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGt                                                                                                                         <  2:54039/143‑1 (MQ=255)
                 tATCCAGCCTGGTGACCAGCTCGCAGTTCCTCACGGTAACAAGGGTGTACTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTa                                                                                                                        <  1:767349/143‑1 (MQ=255)
                 tATCCAGCCGGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTa                                                                                                                        <  2:335930/143‑1 (MQ=255)
                 gactcatcgtGGTGCCAAGCTGGCATGTCGTCACGGGAAGAAGGGTGTACTTTCTAAGATCAACCCGATCTAAGATATGCCTTACGCTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTa                                                                                                                        <  1:216830/134‑1 (MQ=255)
                  gtgcAGTCTGGCGACAAGATGGGAGGTCGTCACGGTAACACGGGTGTAATTTCTAAGGTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGACCCAGCTGGGCGTACCGTCTCGTAt                                                                                                                       <  1:1124787/140‑1 (MQ=255)
                   tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg                                                                                                                      >  1:832056/1‑143 (MQ=255)
                   tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGCAGACATCGTACTGAACCAGCTGGGCGTACCGTCCCGTATg                                                                                                                      >  1:231819/1‑143 (MQ=255)
                     cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                    <  1:458749/143‑1 (MQ=255)
                          tGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                               <  2:1251946/143‑1 (MQ=255)
                          tGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                               >  1:1004331/1‑143 (MQ=255)
                          tGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCAAAGTCCATGTGATTGCTATATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                               <  2:623983/143‑1 (MQ=255)
                            gTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGt                                                                                                             >  1:1262354/1‑143 (MQ=255)
                            gTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGt                                                                                                             >  2:961727/1‑143 (MQ=255)
                            gTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTACTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGt                                                                                                             >  1:819204/1‑143 (MQ=255)
                                cAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGa                                                                                                         <  1:1220728/143‑1 (MQ=255)
                                   gATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATcg                                                                                                      >  1:545372/1‑142 (MQ=255)
                                     tcccAGGTCCTCACTGTAACAAGCGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                    <  2:29346/140‑1 (MQ=255)
                                       gCAGGTCGTCACGGTAACAAGGGTGTAATTGCTATGATCAACCGGATCGAAGATATGCCTTACGATGAAAACGTTACGCTGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGa                                                                                                  <  1:467554/143‑1 (MQ=255)
                                         aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                                <  1:1285224/143‑1 (MQ=255)
                                         aGGGCGGCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATACGCCTAACGTCGCAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                                <  1:1014558/143‑1 (MQ=255)
                                                cACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGCTCCTCGCAACCCAcca                                                                                         >  1:1048670/1‑142 (MQ=255)
                                                 aCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAc                                                                                                                                 <  2:588262/102‑1 (MQ=255)
                                                 aCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAc                                                                                                                                 >  1:588262/1‑102 (MQ=255)
                                                  cGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                       <  2:528241/143‑1 (MQ=255)
                                                      aaCAAGGGTGTAAGGTCTGAGATCAACCCGATCGAAGATATGCCTCACGATGAGAGCGGTACGCCGCTAGACATCGTACTGAACCAGCTGGGAGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAt                                                                                   <  1:1292865/143‑1 (MQ=255)
                                                      aaCAAGGGGGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAt                                                                                   <  1:778374/143‑1 (MQ=255)
                                                       acagctgtgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGATCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATg                                                                                  <  1:141967/137‑1 (MQ=255)
                                                         aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGc                                                                                >  2:93177/1‑143 (MQ=255)
                                                                tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                         <  2:256068/143‑1 (MQ=255)
                                                                 aaTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGCGTATGGCTGCGAAAg                                                                        <  1:1140379/143‑1 (MQ=255)
                                                                      ttAAGAACAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCTAAAGGTATc                                                                   <  2:949672/142‑1 (MQ=255)
                                                                      cTAAGATCAACCCCAGCGAAGCTATGCCCTACGATGAAAACGGTACCCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTACAATAGAGGAAATGGACCAGGGTATGGCTGCCAAAGGTATc                                                                   <  1:969067/143‑1 (MQ=255)
                                                                       tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCg                                                                  >  1:997938/1‑143 (MQ=255)
                                                                       tAAGATCAACCCGATCGAAGATATACCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTCAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGGCAGAGCGTCGAACCCCACCCGGGGATGGATGCGCAAAGTagct                                                                  >  1:710245/1‑140 (MQ=255)
                                                                        aaGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCgg                                                                 <  2:997938/143‑1 (MQ=255)
                                                                          gATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCg                                                               >  2:649494/1‑143 (MQ=255)
                                                                           aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                              <  1:93177/143‑1 (MQ=255)
                                                                              gaCCCGGTCGAAGCTATGCCTGAGGATGGAAACGGTACGTTGGTAGACTGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCTCCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                           <  1:401750/142‑1 (MQ=255)
                                                                              aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGACACCCACCTGGGTATGGCTGCGAACGGCATCGGCGAcca                                                           >  1:1250922/1‑141 (MQ=255)
                                                                               aCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGGCAAg                                                          >  1:564056/1‑143 (MQ=255)
                                                                               aCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAg                                                          <  2:1090522/143‑1 (MQ=255)
                                                                                 ccGATCGAGGAGATGTCTTGCGATGAACACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAACCCCAACTGCGTATGCCTGCGAAAGGTATCGGCGACAAGAt                                                        <  1:482940/143‑1 (MQ=255)
                                                                                 ccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                        >  1:862048/1‑143 (MQ=255)
                                                                                  cGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAGCTGGGTATGGCTGCGAAAGGTATCGGCGGCAAGATc                                                       >  2:464287/1‑143 (MQ=255)
                                                                                    aTCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCaa                                                     <  1:1350617/143‑1 (MQ=255)
                                                                                    aTCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGAACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCGCGAAACCCACCTGGGTATGGCGGCGAAAGGAATAGGGGACAAGATcca                                                     >  2:1113520/1‑141 (MQ=255)
                                                                                            atatGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATg                                              <  2:662770/143‑1 (MQ=255)
                                                                                            atatGCCTTACGACGGAAGCGGTGCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATg                                              <  1:1268006/143‑1 (MQ=255)
                                                                                             tatGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGc                                             <  2:867654/143‑1 (MQ=255)
                                                                                             tatGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGc                                             <  2:1250602/143‑1 (MQ=255)
                                                                                                  cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACCAGATCAACGCCATGCTGaaa                                        >  2:650285/1‑143 (MQ=255)
                                                                                                  cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAACTGGGCGAACCGTCTCGTATAAACATCAGTCAGATCCTCGAAACCCACCTGGGGATGGCTGCGAACGAAACCGGCGACGAGAAACACGCCATGCTGaaa                                        >  1:502731/1‑143 (MQ=255)
                                                                                                       gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGCAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAccgca                                   >  2:94353/1‑143 (MQ=255)
                                                                                                          gAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAGCAGCGCCATGCTGAAACAgcagca                                >  1:214747/1‑143 (MQ=255)
                                                                                                             aaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTAAGATCCTCGAGACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGACCACACCCAGGCGGATGCAGCAGCaaga                             >  1:212317/1‑143 (MQ=255)
                                                                                                               cGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGACAGGTCTCGGCGACAAGATCAACGCCATGGTGAAACAGCAGCaagaag                           >  2:899759/1‑143 (MQ=255)
                                                                                                                 gTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACCGCAGCCAGAAGTc                         >  1:400907/1‑143 (MQ=255)
                                                                                                                  tACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGACAGGTATCGGCGACAAGATCAACGCCATGCTGAACCAGCGGCAAGAAGTcg                        >  2:544809/1‑143 (MQ=255)
                                                                                                                  tACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                        <  2:1221285/143‑1 (MQ=255)
                                                                                                                    cGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCACCGCCATGCTGCAACAGCAGCAAGAAGTcgcg                      >  2:277150/1‑143 (MQ=255)
                                                                                                                     gCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGCAAGCTATCGGCGACCACATCCACGCCACGCGGAAACAACCGCCAGAAGTcgaca                     >  1:935741/1‑140 (MQ=255)
                                                                                                                       cGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGaaa                   >  2:781552/1‑143 (MQ=255)
                                                                                                                             aCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc             >  1:1032820/1‑143 (MQ=255)
                                                                                                                               aTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGGAGTAGCGAAACTGCGCGa           >  2:771180/1‑143 (MQ=255)
                                                                                                                                   tACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGCTCAACGTCTTACGAAAACAGCAGCAAGACGTCGCGAAACTGCGCGAATTc       >  1:280337/1‑143 (MQ=255)
                                                                                                                                     cTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGGGAATTCAt     >  2:72455/1‑143 (MQ=255)
                                                                                                                                       gAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGACAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGACACTGCGCGCATTCAGcc   >  1:563348/1‑143 (MQ=255)
                                                                                                                                        aaCCGGCTTGGCGTACCGTCTCGTATGAACATCGGTGAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCa  <  2:1022220/143‑1 (MQ=255)
                                                                                                                                        aaCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGCTAACCTCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCa  >  2:1339852/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                          
CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184403‑4184680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: