Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,132,7870AC96.7% 179.3 / NA 62I204S (ATC→AGC) wcaAputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (27/32);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132649‑2132926
                                                                                                                                          |                                                                                                                                            
aTCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGCTCTGCATTTCACCGTGATTGATATGCAGGCTCTg                                                                                                                                          >  2:1054480/1‑143 (MQ=255)
  cTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGTGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCg                                                                                                                                        >  2:500194/1‑143 (MQ=255)
    ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCCTTTCCCCGTGATTGATATGCAGGCTCTGCgcc                                                                                                                                      >  2:47466/1‑141 (MQ=255)
    ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                      >  1:953042/1‑143 (MQ=255)
      tAGAGGGTGAACAGTTGATATTTGTTGCTGGCGCGGTCGAAGTTGTCTTTGTGCTTGGGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                    <  2:195814/143‑1 (MQ=255)
      tAGAGGGTCTTCCGCTGATATCTTTTGCTGGCGCGGTCGATTTTGTCTTTGTGCTTGGGGTAATAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                    <  2:760177/143‑1 (MQ=255)
           ggTGAACAGCTGATATTTTTTGCTGGCGCGGCCGAATCCGCTTTCGTGCTTGCGGTAAAACTGGAAGTACCCGGAGAAGTTTTTCGGTGACGAGGTGATCTTCATTTCACCGCGATTGATCTGCCGGGTCGGCGTCGCCtctt                                                                                                                               >  2:951852/1‑143 (MQ=255)
             tAAACAGCTGATATTATTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGCAGTACCCGGAGCATTTTTTCGGTGGCGAGGTGATCTGCATTTCACCGTGATGGACATGCGGGCGCGGCGTCGCCtctgct                                                                                                                             >  2:957553/1‑143 (MQ=255)
              aagcAGCCGATTTTTTGTGCTGGCGCGGTCGAATGTGTCTTTGTGCTTGCGGTAGAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                            <  2:191328/140‑1 (MQ=255)
              aaaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                            <  1:8989/143‑1 (MQ=255)
                aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACTAGGTGATCTGCATTTCACCGTGATTGATTTGCAGGCTCTGCGTCGCCtcttcttct                                                                                                                          >  2:106174/1‑143 (MQ=255)
                  aGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTATCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACttt                                                                                                                        <  2:546127/143‑1 (MQ=255)
                   gCTGATATTTTTTGCTCGCGCGGTCGAATTCGTCTTTGTGCCTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc                                                                                                                       <  1:443181/143‑1 (MQ=255)
                     tggtagttgttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGCGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                     <  1:491176/134‑1 (MQ=255)
                     tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAGTTTCCa                                                                                                                     >  1:407759/1‑143 (MQ=255)
                     tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                     <  1:392105/143‑1 (MQ=255)
                      gATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCAc                                                                                                                    >  1:764239/1‑143 (MQ=255)
                        tatTTTTCGCTGGCGCGGTCGAATTTGTCTTTGTGCTCGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACgg                                                                                                                  <  1:969504/143‑1 (MQ=255)
                              cagcttgtgcgtccGAAGTTGGCCTTGTGCCTGCCGTAAAAGGGGAAGTTGCCGGATAAGTTTTTCGGTGACTGTGTGAGCTGGATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCATCTTCTACTTTCCACGGTTCGcc                                                                                                            <  2:849500/130‑1 (MQ=255)
                                        ggTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTGCGCCGTACTccccc                                                                                                  >  1:65262/1‑143 (MQ=255)
                                          tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCCGGCTCTGCGTCGCCTCTTCTACTGTCCCCGGTTCGCCGGACTccaccac                                                                                                >  2:442359/1‑143 (MQ=255)
                                          tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTCCTTTCCACGGTTAGCCGTACTcaaccac                                                                                                >  2:840693/1‑143 (MQ=255)
                                          tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccac                                                                                                >  2:361184/1‑143 (MQ=255)
                                            gAATTTGTGTTTGTGGGTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacca                                                                                              <  1:47466/143‑1 (MQ=255)
                                              aTTTGGCTTTGTGCTTGCGGTAAAGGGGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATc                                                                                            <  1:323886/143‑1 (MQ=255)
                                               tttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtt                                                                                                                         >  1:801778/1‑113 (MQ=255)
                                               tttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtt                                                                                                                         <  2:801778/113‑1 (MQ=255)
                                                 tGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGc                                                                                        <  1:608068/143‑1 (MQ=255)
                                                     tttGTGCTTGCGGTGACAGTGGTAGTACCCGGCGAGTTTTTTCGGTGGCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa                                                                                    <  2:48263/143‑1 (MQ=255)
                                                     tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATGTGCATTTAACAGTGATTGATATGCAGGATCTGCGTCGCGTCTTCTACGTTCCACGCTTAGCCGCACGCCACCACCACCCGCACGa                                                                                    >  2:451502/1‑143 (MQ=255)
                                                     tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCATGGTTCGCCGTACTCCACCACCATCCGCAGGa                                                                                    >  2:877608/1‑143 (MQ=255)
                                                         tgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTAGGTGATGAGGTGATATGCATTTCACCGTGATTGATATGAAGGCTCTGCGTCGCCTCTTCTACTTTCCACCGTTAGTCGTAATCCACGCCCATCTCGCGGAAGAt                                                                                >  2:599559/1‑143 (MQ=255)
                                                         tgCTGGCGGTAAAAGGGGAAGTACCCGGAGAATGTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGAt                                                                                <  2:847457/143‑1 (MQ=255)
                                                           cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCGTCCGCAGGAAGATGt                                                                              >  1:129422/1‑143 (MQ=255)
                                                             ttcGGTATAAGTGGAGGTCGCCGGAGAATTTTTTCGGTGAGGAGGTGAGCTGCAGTGCACCGGGATTGATATGCAGGCGTTGCGTCGCCTCTTCCACTTTCCACGGTTCTCCGTACTCCACCACCATCCGCAGGAAGATGTCg                                                                            <  2:1005985/141‑1 (MQ=255)
                                                              gCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGt                                                                           <  1:951852/143‑1 (MQ=255)
                                                              gCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGt                                                                           <  1:1045746/143‑1 (MQ=255)
                                                                ggTAAAGGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATAGGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTaa                                                                         <  2:126493/143‑1 (MQ=255)
                                                                    aaaGTGGAAGTACCCGGAGAATTTGTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCt                                                                     <  1:898377/143‑1 (MQ=255)
                                                                      aGTTGAACTACGCGGAGCACTTCTTCGGGGACGCGGTAACCTGCATTTCACCTTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt                                                                   <  1:500194/143‑1 (MQ=255)
                                                                       gTGGTAGTACCCGGAGACTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                                  <  1:614398/143‑1 (MQ=255)
                                                                       gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCGTAACCCtgcg                                                                  >  2:449931/1‑141 (MQ=255)
                                                                       gTGCAAGTACCCGGAGACTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                                  <  2:128008/143‑1 (MQ=255)
                                                                        tGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTGATGCTGTGc                                                                 >  2:960259/1‑143 (MQ=255)
                                                                                  cGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGt                                                       >  1:156403/1‑143 (MQ=255)
                                                                                      gaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGAGTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCgg                                                   <  2:787933/143‑1 (MQ=255)
                                                                                          gttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCACCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc                                               <  2:503885/142‑1 (MQ=255)
                                                                                            tttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTCTCCATTAGGTATCGa                                             >  2:575690/1‑143 (MQ=255)
                                                                                                 gggACGAGGTGATCTGCATTTCACCGTGAGTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                        <  2:380098/141‑1 (MQ=255)
                                                                                                  tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaac                                       <  1:818530/143‑1 (MQ=255)
                                                                                                        ggTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGCACAAACACTCtt                                 >  2:418122/1‑143 (MQ=255)
                                                                                                         gTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt                                <  1:367742/143‑1 (MQ=255)
                                                                                                           gATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCTGCTTTCAGTTCGGTATCGAACCAACACCCTTTgc                              >  1:728853/1‑142 (MQ=255)
                                                                                                            aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCGGTTCTCCACCCCCATCCGCGCGAAGCTGCCGTCAGCCTGCGCCGCCCTCCATTCGGTATCGagcacagacgctgcgac                             >  2:425334/1‑130 (MQ=255)
                                                                                                            aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCCCCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTACCGAACCAACACTCTCTGaa                             >  2:299620/1‑143 (MQ=255)
                                                                                                            aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAACCCCTCTTTgca                             >  1:604272/1‑141 (MQ=255)
                                                                                                             tcggCATTTGACCGTGATTGATGTGCAGGATCTTCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCACCATCCGCAGTAAGATGTCGTCATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa                            <  2:940916/140‑1 (MQ=255)
                                                                                                                                 atatGCAGGCTCTGCCTCTCCTCTTCTACTTTCCACGGTTCACCATACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc        <  1:599559/143‑1 (MQ=255)
                                                                                                                                  tatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGCACAAACACTCTTTGAAACGCCATGCCCAGGTATAGccc       >  1:1044051/1‑143 (MQ=255)
                                                                                                                                      cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCATGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGt   >  2:10612/1‑143 (MQ=255)
                                                                                                                                       aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt  <  2:156403/143‑1 (MQ=255)
                                                                                                                                       aGGCTCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCGTACCCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt  <  1:1079871/143‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
ATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132649‑2132926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: