Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,547,879 Δ1 bp coding (479/3048 nt) fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,547,8790C.95.2% 250.9 / 5.0 64coding (479/3048 nt)fdnGformate dehydrogenase‑N, alpha subunit, nitrate‑inducible
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base . (31/29);  minor base G (1/2);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGGTTCTGACAAATGGCAGCGCATTAGCTGGGAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTCTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGGCGCGCGTCTGACACGGACCAACGGTAGCAAGTCTTGCT  >  NC_000913/1547746‑1548015
                                                                                                                                     |                                                                                                                                        
ccAGGTTCTGACAAATGGCAGCGCATTAGCTGGGAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGAACGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATg                                                                                                                                >  1:78384/1‑143 (MQ=255)
 cAGGTTCTGACAAATGGCAGCGCATTAGCTGGGAAGAAACATTCTCCCGGATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGc                                                                                                                               >  1:223134/1‑143 (MQ=255)
    gTTCTGACAAATGGCAGCGCATTAGCTGGGAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCtgt                                                                                                                            <  2:149005/143‑1 (MQ=255)
      tCTGACGCATGGCCGAGCATTATCGTGGAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATGGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCtgtgt                                                                                                                          <  2:558897/143‑1 (MQ=255)
      acTGACAAATGGCAGCGCAGTAGCTGGGAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCGGACCGTGCCGCTAACATTATTGTTAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCtgtgt                                                                                                                          <  1:860803/142‑1 (MQ=255)
         gACAAATGGCAGCGCATTAGCTGGGAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGcc                                                                                                                       >  2:468059/1‑143 (MQ=255)
          aCAAATGGCAGCGCATTAGCTGGGAAGAAGCATTATCCCGAATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATGTAATAGAACGAGAAGGGCGTAACGGGAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCt                                                                                                                      >  1:734155/1‑143 (MQ=255)
             aaTGGCAGCGCATTAGCTGGGAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCg                                                                                                                   <  1:981093/143‑1 (MQ=255)
              aTGGCAGCGCATTAGCTGGGAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTGTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCgg                                                                                                                  <  2:937666/143‑1 (MQ=255)
               tGGCAGCGCATTAGCTGGGAAGAAGCATTCTCCCGTATTGAGAAGCAAATGACAACAGACCCCGCCGCCGGCGTTACTGGAAATGACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGt                                                                                                                 >  1:546656/1‑143 (MQ=255)
               tGGCAGCGAACTAGCCGGGAAGAAGCATTCTCCCGTATTGCGAAGCGGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGt                                                                                                                 <  1:1042362/143‑1 (MQ=255)
                 gCAGCGCATTAGCGGGGAAGAAGCATTCTCCCGTATGGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGc                                                                                                               <  2:428760/143‑1 (MQ=255)
                   aGCGCATTAGCTGGGAGGAAGCATGCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTGTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCa                                                                                                             <  1:1099826/143‑1 (MQ=255)
                   aGCGCATTAGCTGGGAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAGGCTGGCCGTGACGCTAACTTGATTGAACAGAACGAGCAGGGCGTAACGGGAAACCGTTGGCTGTTACCGGCATGCtgtgtcgcggcgctgccc                                                                                                             >  2:1073308/1‑130 (MQ=255)
                        agtAGCTGGGAAGAAGCAGTCCCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAAc                                                                                                        <  1:184507/141‑1 (MQ=255)
                            gCTGGGAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTATATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTTCGCTGCCAGCCACGacc                                                                                                    >  1:395745/1‑141 (MQ=255)
                              tGGGGAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAAcc                                                                                                  <  2:509528/143‑1 (MQ=255)
                              tGGGAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGGACGAGCAGGGCGTAACGGTAAACCGTCGAGTTGTACCGGTACCGTGTGGCCCTCCGGTGCCAGCAAGGacacg                                                                                                  >  2:1178324/1‑142 (MQ=255)
                              tGGGAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAAcc                                                                                                  >  1:623788/1‑143 (MQ=255)
                               gggAAGAAGCATCCGCCCGAATTGCGAAGCTAATGAAAGCTGAGCGTGACGCTAACTGTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTGGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCg                                                                                                 <  2:666053/143‑1 (MQ=255)
                                ggAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCCAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCgg                                                                                                <  1:459667/143‑1 (MQ=255)
                                  aagaagCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGa                                                                                              <  2:275888/143‑1 (MQ=255)
                                    gaagCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGACCCCGGGATg                                                                                            >  2:1162739/1‑143 (MQ=255)
                                        cATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCACCGACACCGGGAGGCTGa                                                                                        >  2:608251/1‑143 (MQ=255)
                                        cATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGa                                                                                        >  1:661001/1‑143 (MQ=255)
                                          tgctcCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGAcc                                                                                      <  1:1150365/141‑1 (MQ=255)
                                             tcCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAg                                                                                   >  1:872881/1‑143 (MQ=255)
                                               ccGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGACAAGAACGAGCAGACCGACACGGTAAACCGTTGCCTTTTACCGGTATGCTGTGTGCCTCCGGCGCCCGCACCGCAACCGGGATGCTGACCgagac                                                                                 >  2:195203/1‑142 (MQ=255)
                                                cGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAACAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGGCCGCAAAGAAACCGGGATGCTCACACAGaaa                                                                                >  2:55139/1‑143 (MQ=255)
                                                  tATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGACGCTGACCCAGACAtt                                                                              >  2:547604/1‑143 (MQ=255)
                                                   agtgcgtggcgggtgaAAGCTTGTCGTGACGCTAAGTTGATTGAATGTGACGAGCAGGGTGTAGCGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAATCCGGGATGCTGACCCAGAAAttt                                                                             <  2:95272/130‑1 (MQ=255)
                                                       cGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAACGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCGGAAAATTGccc                                                                         >  1:391949/1‑143 (MQ=255)
                                                       cGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAGCGGAACCGGGATGCTGACCCAGAAAGTTcccc                                                                         >  2:863649/1‑143 (MQ=255)
                                                       cGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGccc                                                                         <  1:636269/143‑1 (MQ=255)
                                                         aaGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGc                                                                       <  1:256197/143‑1 (MQ=255)
                                                               atgaAAGCTGACCGTGACGCTAACTTTATTGAAAAGAAGGAGCAGGGCGTAACGGTAAACCGGTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGGCCCGGAGAGTTGCCCGCTCCCTc                                                                 >  1:1022537/1‑143 (MQ=255)
                                                               atgaAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCGGCACCGACACCGCGATGCCGACCCCGCAATTTGCCCGCTcccca                                                                 >  2:436269/1‑141 (MQ=255)
                                                                     gCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATg                                                           >  2:681154/1‑143 (MQ=255)
                                                                     gCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAAGTTGCCCGCTCCCCCGGGATg                                                           >  2:975891/1‑143 (MQ=255)
                                                                       tGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAACCCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCt                                                         >  1:684841/1‑143 (MQ=255)
                                                                         tccGTGACTCTAACTTTATTCAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTgg                                                       <  2:160617/142‑1 (MQ=255)
                                                                           cGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTAGCTTTTACCGGGATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATTCTGAAACAGAAATTTTACAGCTCCCTAGGGATGCTGGCg                                                     >  1:108009/1‑143 (MQ=255)
                                                                            gTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCgg                                                    <  1:1036833/143‑1 (MQ=255)
                                                                            gTGACGCGAACTTGATTGAAAAGAACGAGCAGGGCGTAATGGTAAACCGTTGGCTTTTACCGGTATGCGGTGTGCCTCCGGGGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCgg                                                    <  1:466064/143‑1 (MQ=255)
                                                                              gACGCTAAATGTAGTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGAGGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTa                                                  <  2:1164890/143‑1 (MQ=255)
                                                                                cccTAACGTGATTGCAAAGGACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGa                                                <  2:1009013/141‑1 (MQ=255)
                                                                                   tAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACaa                                             >  1:702218/1‑143 (MQ=255)
                                                                                    aaCTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCACCGAAACCGGGATGCTGACCCAGGAATTTGCCCGCCCCGTCGGGATGCTGGCGGCACACAAc                                            >  2:369848/1‑143 (MQ=255)
                                                                                           ttGAAAAGAACGAGCAGGGCGTAACGCTATACTGTTGGCTTTTACCCGTATGCTGTGTCCCTCCGGTGTCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCTGCTCCCTCTGGATGCTGGCGGTAGACAACCAGgcgc                                     <  2:831268/143‑1 (MQ=255)
                                                                                             gAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGgcgcgc                                   >  1:185295/1‑143 (MQ=255)
                                                                                              aaaaGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACCACCAGGcgcgcg                                  >  2:752654/1‑143 (MQ=255)
                                                                                              aaaaGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGATGAGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTACACGCACCCTCAGGCTGCAGTCGGTCGACAACCAGCcgcacg                                  >  2:1077154/1‑143 (MQ=255)
                                                                                               aaaGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTTCGGTGCCAGCAACGAAACCGGGATGGTGACCCAGAAGTTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGGcgcgcgg                                 >  2:586166/1‑142 (MQ=255)
                                                                                               aaaGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGGCGCGCGt                                 <  2:661001/143‑1 (MQ=255)
                                                                                                   aaCGAGCAGGGCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGGGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGGCGCGCGTCTGa                             <  1:880005/143‑1 (MQ=255)
                                                                                                   aaCGAGCAGGGCGTAACGGTAAACCCTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGGCGCGCGTCTGa                             <  2:409514/143‑1 (MQ=255)
                                                                                                         cggggggttgcGGTAAGCCGTTGGGTTGTGCCGGGATGCGGTGTGCGTGCGGTGGCAGCAACGAAACCGGGATGAAGACCGAGAAATTTGCCCGCGCCGTCGGGATGCTGGCGGTAGACAACCAGGCGCGCGTCTGACACGGa                       <  2:887620/133‑1 (MQ=255)
                                                                                                            ggCGTAACGGTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTCGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGACGCGCGCCTGACACGGACCa                    >  2:476557/1‑143 (MQ=255)
                                                                                                               ggaaCGGTAAACCGTTGGCTTTTACCGGTATGCTGTGGGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGGCGCGCGTCTGACACGGACCAACg                 <  1:512462/141‑1 (MQ=255)
                                                                                                                  ccGGTAAACGGTGGGCTTGTAGCGGTGTGCTGTGTGCCTCCGGTGCCAGCAACGAAATCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGGCGCGCGTCTGACACGGACCAACGGTa              <  2:752456/142‑1 (MQ=38)
                                                                                                                     gTAAACCGTTGGCTTTTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAAGGAAACAGGGATGCTGACCCAGAAATTTGACCGCGCCCTCGGGATGCTGGCGGTAGACAACCAGGCGCGCCTCTGACACCGACCCGCGgcagca           >  2:321865/1‑143 (MQ=255)
                                                                                                                        aaCCGTTGGCTGTTACCGGTATGCTGTGTGCCCCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGGCGCGCGTCTGACACGGACCAACGGTAGCAAGt        <  1:276518/143‑1 (MQ=255)
                                                                                                                            gTTGGCTTTGACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGGCGCGCGTCTGACACGGACCAACGGTAGCAAGTCTTg    <  1:468059/143‑1 (MQ=255)
                                                                                                                              tGGCTTTCACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGGCGCGCGTCTGACACGGACCAACGGTAGCAAGTCTTGCt  <  1:398445/143‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                        
CCAGGTTCTGACAAATGGCAGCGCATTAGCTGGGAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGACCGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTCTACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTTGCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGGCGCGCGTCTGACACGGACCAACGGTAGCAAGTCTTGCT  >  NC_000913/1547746‑1548015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: