Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 962,982 G→C V330L (GTT→CTT)  rpsA → 30S ribosomal subunit protein S1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913962,9820GC98.9% 279.4 / NA 87V330L (GTT→CTT) rpsA30S ribosomal subunit protein S1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (41/45);  minor base T (0/1);  total (41/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACT  >  NC_000913/962843‑963119
                                                                                                                                           |                                                                                                                                         
cgTGACCAACCCGACCGCCTACGGCTGCTTCGTTGAAATCGAAGAAGCCGGTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTa                                                                                                                                        <  2:877952/143‑1 (MQ=255)
     ccAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGtt                                                                                                                                   >  2:79802/1‑143 (MQ=255)
      cAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTc                                                                                                                                  >  2:38764/1‑143 (MQ=255)
      cAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTACGGTTc                                                                                                                                  >  2:1128052/1‑143 (MQ=255)
       aaCCTGTCCGACTACGGTTGCTGCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCt                                                                                                                                 <  2:1110916/143‑1 (MQ=255)
       aaCCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCt                                                                                                                                 <  1:554167/143‑1 (MQ=255)
       aaCCTGACCGACTACGGCTGCTGCGTGGAAGTCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCt                                                                                                                                 <  2:280051/143‑1 (MQ=255)
           tGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAGCGTTGACAATGAAGGGGCACTTACGGTTCTAGat                                                                                                                             >  2:1077795/1‑143 (MQ=255)
            gACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGata                                                                                                                            >  2:1041331/1‑143 (MQ=255)
                 aCTACGGCTACTTCGTATATAGCGAAGAAGGCGTTGGAGGCCGGGTACACGTTTCCGAAATGGACTGGACCAACAACCCCCTCCTCCCGTCCAAAGTTTTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATcgac                                                                                                                       <  2:708930/143‑1 (MQ=255)
                 aCTACGGCGGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATcgac                                                                                                                       <  1:172025/143‑1 (MQ=255)
                  cTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATcgacg                                                                                                                      <  1:476007/143‑1 (MQ=255)
                  cTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGCACTGGACCAACAAAAACATCCACCCGTCCACAGTAGTTCACGTTGGCGATGAAGTGGAACTTATGGTTCTGGATATcgacg                                                                                                                      >  2:803886/1‑143 (MQ=255)
                   tACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATcgacga                                                                                                                     <  1:909354/143‑1 (MQ=255)
                   tACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATcgacga                                                                                                                     >  2:1150655/1‑143 (MQ=255)
                   tACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTCGCTAACGGTGGCGACGTTGTGGCACTCATGACTCTGGATATcgacga                                                                                                                     >  1:537896/1‑143 (MQ=255)
                   tACGGCTGCTGCGATGAAAGCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATcgacga                                                                                                                     <  2:80867/143‑1 (MQ=255)
                   tACGGCGGCTGCGTTGAAATCGAAGAAGGCGTTGAAGGCCGGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATcgacga                                                                                                                     <  2:823703/143‑1 (MQ=255)
                     cGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTTTGGACAGAACACAAACAACAAAACCATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTCATGGTTCTGGATCTCGACgaag                                                                                                                   >  2:522142/1‑143 (MQ=255)
                      ggCTGCTTCGTTGAATTGGGAGTATGCGGTGAAGGCCTGGTACACGTCGCCGAAAGGGACTGTACCAACAAAAACATCCGCCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACgaaga                                                                                                                  <  2:1074992/143‑1 (MQ=255)
                       gctgctTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACgaagaa                                                                                                                 <  1:844250/143‑1 (MQ=255)
                        ctgctTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAAc                                                                                                                <  1:1069616/143‑1 (MQ=255)
                           ctTCGTTGAAATCGAAGAAGGCGTTAAAGGCCAGGTTCGCGTTTCCGAAATGGACTGGACCAAGAGAAACATCCACCCGTCCAAAGTTGGTAACGTTGGCGATGTAGTGGAACTTATGGGTCTGGATATCGACGGAGAAccag                                                                                                             >  2:143945/1‑140 (MQ=255)
                            ttCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAccgccg                                                                                                            >  2:738691/1‑143 (MQ=255)
                              cgttacaaTCGAAGAAGGCGGTGAAGACCCGGTACACGTTTCCGAAATGGACTGGACCAACAAATACATCCACCCTGCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAAcgtcgtc                                                                                                          <  2:885758/137‑1 (MQ=255)
                               gtgtaaaTCGAAGGCGGCGTTGCAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAAcgtcgtcg                                                                                                         <  2:907928/139‑1 (MQ=255)
                               gTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAacgacgacg                                                                                                         >  1:722533/1‑143 (MQ=255)
                                 tGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTa                                                                                                       <  1:38764/143‑1 (MQ=255)
                                  gAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGCACGTCGTCGTAt                                                                                                      >  1:330380/1‑143 (MQ=255)
                                   aaaTCGAAGAAGGCGTTGAAGGCCTGGTACACGTTACCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGCTAACGTTGGCGAAGTAGTGGAACGTAGGGTTCTGGATACCGACGAAGAAcgatgtggaatc                                                                                                     >  2:803028/1‑133 (MQ=255)
                                    aaTCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGAAATCGACGAAGAAcgccggcctaccc                                                                                                    >  2:910459/1‑135 (MQ=255)
                                     aTCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATGTCGACGAAGAACGTCGTAGTAtcgc                                                                                                   >  1:560258/1‑141 (MQ=255)
                                      tCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTcc                                                                                                  >  2:224634/1‑143 (MQ=255)
                                      tCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTCCTGCATAGCGACGACGAACGTCGTCGTAtctac                                                                                                  >  2:69681/1‑141 (MQ=255)
                                       cGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTccc                                                                                                 >  1:1091842/1‑143 (MQ=255)
                                       cGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTccc                                                                                                 >  1:1089870/1‑143 (MQ=255)
                                         aagaagGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGGCGTATCTCCCTg                                                                                               >  1:1146769/1‑143 (MQ=255)
                                            aagTCGGTGAAGGCCTGCTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGt                                                                                            <  2:794482/143‑1 (MQ=255)
                                                cGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGa                                                                                        <  2:1050410/143‑1 (MQ=255)
                                                cGTGGAAGGCCTGGTACACGTGTCCGGAATGGACTGGACCAACAAAAACATCCACCCGTCGAACGTTGTTAGCGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGa                                                                                        <  2:897243/143‑1 (MQ=255)
                                                  ttGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTACCGTTGGCGATGTAGTGGAACTTCTGGTTCTGGATATCGCCGAAGCACGTCGCCGTCTCTCCCCGGGCCGGaaa                                                                                      >  2:1001276/1‑143 (MQ=255)
                                                  gtggaGGACTGGTCCACGCTTCCGGAATGGCATGGACCAACAAACACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGaaa                                                                                      <  2:810232/139‑1 (MQ=255)
                                                    gAAGGCCTGGTCCACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACa                                                                                    <  1:1041331/143‑1 (MQ=255)
                                                    gAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGGTATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACa                                                                                    <  2:1037051/143‑1 (MQ=255)
                                                    gAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACa                                                                                    <  2:725665/143‑1 (MQ=255)
                                                    gAAGGCCGGGTACACGTCTCCGAAGTGGACTGGACGAACAAAAACAACCACCCGTCCAAAGTTGTTAACGTGGGCGATGTAGTCGAACTTATGGTGCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACa                                                                                    <  2:1034767/143‑1 (MQ=255)
                                                     aaGGCCTGGTACACGTTTCCGAAATGGACTGGGCCAACAAAAGCATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGGGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGGCGTATCTCCCTGGGTCTGCAACAg                                                                                   >  2:388926/1‑143 (MQ=255)
                                                       ggCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTg                                                                                 <  1:100116/143‑1 (MQ=255)
                                                        gCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTCTGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACGGTGc                                                                                >  2:273944/1‑143 (MQ=255)
                                                          cTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACAGCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCaa                                                                              <  2:121390/143‑1 (MQ=255)
                                                          cTGGTACACGTTTCCGAAATGGACGGGACCAACAAAAACATCCGCCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCaa                                                                              <  2:246488/143‑1 (MQ=255)
                                                              tACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCt                                                                          <  1:177310/143‑1 (MQ=255)
                                                                 acGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAAc                                                                       >  2:323911/1‑143 (MQ=255)
                                                                   gTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAACGTTGTTAACGTTGGCGATGTAGTGGAACCTATGGCTCTGGATATCGACGAAGAACGTGGTAGTCTATCCCTGTGGCTGAAACAGTGCGCAGCCAAccc                                                                     >  2:219683/1‑143 (MQ=255)
                                                                     ttCCGAAATGGCGTGGACCAACAAAAACATCCTCCCGTCACTTGTTGGTGTGCGTGGCGATTTAGTGTAATTTATCGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGt                                                                   <  1:1114362/143‑1 (MQ=255)
                                                                       ccGAAATGGACTGGACCAACGAAAACATGCACCCGTCCAAAGTTGTTAACGTGGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTgg                                                                 <  2:430244/143‑1 (MQ=255)
                                                                        cGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGACGAACGTCGCCGTATCTCCCTAGGACTGAAACAGTACAAACCTAACCCgcagc                                                                >  2:282512/1‑139 (MQ=255)
                                                                        cGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGGCGTACCCCCCCGGGGCTGAAACAGCGCAAAGCTACCCCGTGgc                                                                >  2:346452/1‑143 (MQ=255)
                                                                         gAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGCTGTAGTGGACCTTAGGGTTCTGAATATCGACGAAGAACGTCGTCGTATCTCCCTGCGGCTGAAAACGTGCAAAGCTAACCCGTGgca                                                               >  2:35557/1‑143 (MQ=255)
                                                                            attgccgggacCAACGAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGgcagca                                                            <  2:269653/136‑1 (MQ=255)
                                                                            aTGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGgcagca                                                            >  1:1008931/1‑143 (MQ=255)
                                                                               gactggagCAACAAAAACGTCCACCCGGCCAAAGGTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGAGGCAGCAGtt                                                         <  1:786848/143‑1 (MQ=255)
                                                                               gactggacCAACAAAAACATCGTTCCGTCCAAATGTGTTAACGTTAGCGTTGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAGACAGTGCAAAGCTAACCCGTGGCAGCAGtt                                                         <  1:733092/143‑1 (MQ=255)
                                                                                  tggtcccataacaaCCTCCGCCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGGCGTCGTATCTCCCTGGGTCTGAAACAGTGAAAACCTAACTCGTGGCAGCAGTTcgc                                                      <  2:474437/131‑1 (MQ=255)
                                                                                   ggacCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTcgcg                                                     >  1:528116/1‑143 (MQ=255)
                                                                                     acCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGa                                                   >  1:494876/1‑143 (MQ=255)
                                                                                     acCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACACGTAGCAGCAGTTCGCGGa                                                   >  1:135812/1‑143 (MQ=255)
                                                                                       caacaaAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGaaa                                                 >  1:798838/1‑143 (MQ=255)
                                                                                        aacaaAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACTAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCCGCAGTTCGCGGAAAc                                                >  1:440945/1‑143 (MQ=255)
                                                                                           aaaaaCATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCca                                             >  1:18694/1‑143 (MQ=255)
                                                                                           aaaaaCATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGGGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCca                                             <  1:663122/143‑1 (MQ=255)
                                                                                            aaaaCATCTACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGCCGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCcac                                            <  1:554832/143‑1 (MQ=255)
                                                                                            aaaaCATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCACAATGCCCGCTAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCcac                                            <  1:542061/143‑1 (MQ=255)
                                                                                             aaaCATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCAGTGGCAGCAGTTCGCGGAAACCcacc                                           >  1:242443/1‑142 (MQ=255)
                                                                                                 aggcgtgcGTCCGAAGTTGTGAACGTTGGCGAGGTGGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCAcaacaa                                       <  2:690859/136‑1 (MQ=255)
                                                                                                 aTCCACCCGTCCAAAGTTGTTAACGTTGGCGACGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCAcaacaa                                       >  2:614510/1‑143 (MQ=255)
                                                                                                   ccACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCGGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAgg                                     <  2:118415/143‑1 (MQ=255)
                                                                                                     aCCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGc                                   >  1:1074155/1‑143 (MQ=255)
                                                                                                            cAAAGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCgtg                            <  1:943578/143‑1 (MQ=255)
                                                                                                             aaaGTTGTTAACGTTGGCGATGTAGTGGAACTTATGGTTCTGAATATCGACGAAGAACGTCATCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCTGTGGCAGCAGATCGCGGAAACCCAcaacaaagaagagcgagt                           >  2:80836/1‑131 (MQ=255)
                                                                                                                 ttgttAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAACGGCGACCGCGTTGaa                       >  2:1059190/1‑143 (MQ=255)
                                                                                                                  tgttAACGTTGGCGATGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAg                      <  1:1150655/143‑1 (MQ=255)
                                                                                                                             gCGAGGTAGTGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAg           <  1:164345/143‑1 (MQ=255)
                                                                                                                              cGATGTAGGGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGt          <  2:916724/143‑1 (MQ=255)
                                                                                                                                      tGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACt  <  2:18694/143‑1 (MQ=255)
                                                                                                                                      tGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACt  <  2:1089870/143‑1 (MQ=255)
                                                                                                                                      tGGAACTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTAGCGGAAACCCACATCATGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACt  <  2:1091842/143‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
CGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACT  >  NC_000913/962843‑963119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: