Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,677,913	T→C	intergenic (‑10/‑514)	pntA ← / → ydgH	pyridine nucleotide transhydrogenase, alpha subunit/DUF1471 family periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,677,913	0	T	C	100.0%	181.6 / NA	61	intergenic (‑10/‑514)	pntA/ydgH	pyridine nucleotide transhydrogenase, alpha subunit/DUF1471 family periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/31);  total (30/31)

CTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGC  >  NC_000913/1677773‑1678052                                                                                                                                             |                                                                                                                                            cgtGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTGTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGTATGATATTCCCCTc                                                                                                                                           <  2:408900/141‑1 (MQ=255)  ttGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCATTTCGCATGCTATTCCCCTcc                                                                                                                                          >  2:323184/1‑143 (MQ=255)  ttGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTcc                                                                                                                                          >  2:993438/1‑143 (MQ=255)        gTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTGTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCGCTTGGTATGCCAATTCGCATGATATTCCGCTCCACCggg                                                                                                                                    >  2:55167/1‑142 (MQ=255)         ttGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGtt                                                                                                                                   <  1:9515/143‑1 (MQ=255)         ctgcccctctccgcTCTCTACCGCGTCGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGtt                                                                                                                                   <  1:218448/133‑1 (MQ=255)           gACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGgttt                                                                                                                                 >  1:644682/1‑143 (MQ=255)                    cgcTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTgatgat                                                                                                                        >  2:1020977/1‑143 (MQ=255)                    cgcTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCATTGCTGCAACACGGGTTTCATTGCTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATtggtgtt                                                                                                                        >  1:772290/1‑141 (MQ=255)                     gcTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGatgatg                                                                                                                       <  1:1069405/143‑1 (MQ=255)                       tctctACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAGTTCGCATGATATTCCCCTCCATCGGCTTTATTGATGACGGt                                                                                                                     >  2:830355/1‑143 (MQ=255)                         tcgaCCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGGTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGttt                                                                                                                   <  1:293519/140‑1 (MQ=255)                           tgccGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGc                                                                                                                 <  1:621736/141‑1 (MQ=255)                             ccGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCt                                                                                                               <  1:677005/143‑1 (MQ=255)                              cgcgACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTGATTGATGCTGGTTTGCCtg                                                                                                              >  1:298001/1‑143 (MQ=255)                                   cgtcactaCCCCGGTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGGTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCa                                                                                                         <  2:335180/136‑1 (MQ=255)                                     gTAACACCCCGGCTCGGCAGCTGTTCCACTGTTTGTGGCGTTGCTGCAACACGGGTTTCACTGGTTAACCGGTCTCTTGGTATGCCCATTCGCATGATACTCCCCTCCATCGGTTCTATTGATGATGGTTTGCCTGTGTCAgc                                                                                                       >  2:255848/1‑142 (MQ=255)                                           cccAGTTTGGGGAGCTGTTCCTCTGTTTTTGGCGTGGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCGTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATGGATGATGGTTTGCCTGTGTCAGGAGCcac                                                                                                 <  2:419618/143‑1 (MQ=255)                                           cccAGTGCCAGCAGCTGTTCCACGGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTGAACCGTTCTCTCGGTATGCCCATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCcac                                                                                                 <  1:997868/143‑1 (MQ=255)                                           cacAGTTTCAGCAGCTGTTGCACTGTTTGTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGTTATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCcac                                                                                                 <  2:599876/141‑1 (MQ=255)                                            ccAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCcaca                                                                                                >  1:559429/1‑143 (MQ=255)                                            ccAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCcaca                                                                                                >  1:956869/1‑143 (MQ=255)                                            ccAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGGCAGGAGCcaca                                                                                                >  2:935358/1‑143 (MQ=255)                                                   ctgcagcTGTTCCCCTGTCTTTGGCGTGGCCGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCACTTCGCATGATATTCCCCTCCATCGGTTTTATTGATGAGGGTTTGCCTGTGTCAGGAGCCACACAAgctg                                                                                         <  1:30893/141‑1 (MQ=255)                                                   cagcagCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTCAATTGGTTAACCGCTCTCTCAGTCGTTCAATGCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAgctg                                                                                         <  1:44519/143‑1 (MQ=255)                                                   cagcagCGGGTCCACCTTTTTTGCGGTGGCTGCGACACGGGTTTCATTGGTCAACCGTTCTCTTGGTATGCCAATGTGCATGATATTCCCCGCCAACGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAgctg                                                                                         <  2:445646/143‑1 (MQ=255)                                                    atcagcTGTTCCACTGTTTTTGGCGATGATTCACTTCGGTTTTTATTTGTTATCCTTTCTCTTGGTATGCCAATTCGCATGATATGCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAgctgc                                                                                        <  1:487866/141‑1 (MQ=255)                                                       agtggTTCCTCTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGTTTAACCGTTCTCTTGGTATGCCAATTCGCATGATCTTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCa                                                                                     <  2:719882/139‑1 (MQ=255)                                                       agcTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCAGCTCa                                                                                     >  2:971113/1‑143 (MQ=255)                                                         cTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATg                                                                                   >  1:930215/1‑143 (MQ=255)                                                          tGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCGCATGt                                                                                  >  1:158214/1‑143 (MQ=255)                                                            ttCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTAc                                                                                >  1:683650/1‑143 (MQ=255)                                                                   gTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTaa                                                                         <  1:271303/143‑1 (MQ=255)                                                                       ttGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGt                                                                     <  2:724019/143‑1 (MQ=255)                                                                          gCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTTCCCTCCATCGGTTTTATTTATGATGGTTTGCCTGTGTCAGAATCCACACACACTGCTCATGTACGAGCTAAATGTTAc                                                                  >  2:384311/1‑143 (MQ=255)                                                                          gCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATGGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCCCACAAGCTGCTCACGTACGAGCTACATGTTAc                                                                  >  2:985270/1‑143 (MQ=255)                                                                            gtggctgcAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTc                                                                <  2:680556/140‑1 (MQ=255)                                                                            gTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTACATGTTACTc                                                                >  1:823480/1‑143 (MQ=255)                                                                            gTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTc                                                                <  1:1020977/143‑1 (MQ=255)                                                                                 tgcAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAACTGCACCTGCACGAGCTAAATGTTACCCCGATa                                                           >  2:316006/1‑143 (MQ=255)                                                                                       acGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATGCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACCCCGTTAaaataa                                                     <  1:644460/143‑1 (MQ=255)                                                                                          ggTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAAtt                                                  >  1:1105186/1‑143 (MQ=255)                                                                                           gTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTCTTATTGATGATGGTGTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTa                                                 <  2:639699/143‑1 (MQ=255)                                                                                            tttCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAg                                                <  1:882958/143‑1 (MQ=255)                                                                                             ttCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGt                                               <  2:918419/143‑1 (MQ=255)                                                                                                     ttAACCGTTCTCTTGGTATGCCAATTCGCATTATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAACTGTTACTCTGTTAAAATAAATTAGTAACATACg                                       >  1:444685/1‑143 (MQ=255)                                                                                                     ttAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCTGTTAAAATAAATTAGTAACAAACg                                       >  1:1044094/1‑143 (MQ=255)                                                                                                       aaCCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGcc                                     <  2:8689/143‑1 (MQ=255)                                                                                                       aaCCGTTCTCTTGGTAGGCCAATTCGCTTGTTATTCCCCTCCATCGGTTTTATTGATGATTGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGcc                                     <  2:294229/143‑1 (MQ=255)                                                                                                         ccGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGAGGGGTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCt                                   >  1:384393/1‑143 (MQ=255)                                                                                                               tctTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAACTTAGTAACAAACCCCCTATAACg                             >  1:544429/1‑143 (MQ=255)                                                                                                                 ttGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTa                           >  1:670339/1‑143 (MQ=255)                                                                                                                      aTGCCAAGTCGCATGATATTCCCCTCCAGCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGaa                      <  1:480302/143‑1 (MQ=255)                                                                                                                        gCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGaaaa                    >  2:41849/1‑143 (MQ=255)                                                                                                                         ccAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATGAAGTACTgttgtg                   >  1:209341/1‑138 (MQ=255)                                                                                                                         ccAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAAt                   <  2:559429/143‑1 (MQ=255)                                                                                                                           aaTTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTa                 <  1:1011931/143‑1 (MQ=255)                                                                                                                            aTTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTAt                <  1:1102659/143‑1 (MQ=255)                                                                                                                                    tgtatTCCCCTGCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGa        <  2:104102/141‑1 (MQ=255)                                                                                                                                    gagatgccccTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGa        <  1:993438/137‑1 (MQ=255)                                                                                                                                       atTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTTTCATGAGCCCCACAAGCTGCTCATGTACGAGCTAAATGTGATGCCGTTGAACTCAATTAGTAACAAACGCCCTCTCACGTACGGAAAATCCTGCCTGTGATcc     >  2:290737/1‑142 (MQ=255)                                                                                                                                        ttCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTGCGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAACATTATTCATGTGATCTa    >  1:14101/1‑143 (MQ=255)                                                                                                                                          ccccTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAgc  <  1:41849/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                            CTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGC  >  NC_000913/1677773‑1678052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑