| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | 465407 = | 43 (1.030) | 3 (0.070) | 3/274 | 6.0 | 6.4% | coding (141/696 nt) | queC | 7‑cyano‑7‑deazaguanine (preQ0) synthase, queuosine biosynthesis |
| ? | NC_000913 | 465460 = | 46 (1.140) | coding (88/696 nt) | queC | 7‑cyano‑7‑deazaguanine (preQ0) synthase, queuosine biosynthesis | |||||
| Rejected: Coverage evenness skew score above cutoff. | |||||||||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
GTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATAAAAGCGTTATGGTAACGCCTGCGATTAACC > NC_000913/465324‑465599 | gTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGAc > 2:38529/1‑143 (MQ=255) aGGCTACTGGCCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTtc < 2:354927/143‑1 (MQ=255) ggCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCGGCGCGCGTTCGCGTGCCACGTCGATCTCTGCGCGATGCCGCGGACCGTAATCGAACGTCACGCAAGGGACTtca > 2:674367/1‑143 (MQ=255) aCCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACGTCCTAATAttg > 1:128561/1‑143 (MQ=255) ccAGCTCGGTGAGCAGGGTGACATCCAGCGCCTGATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATtgttgt < 1:288729/143‑1 (MQ=255) gcattGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGc < 1:716554/140‑1 (MQ=255) cggtGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTGCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGcc < 2:718759/140‑1 (MQ=255) cGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGCCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCCCGCACTGGACTTCATCATATTGTTGT‑AATGcc > 2:310141/1‑143 (MQ=255) aGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCAc < 1:38529/143‑1 (MQ=255) tgtcccccAGCTCCTTATGCGCGCGTGCCCCCAGGTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAg < 2:473594/137‑1 (MQ=255) tGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑CATGCCTGAACCAGACAg < 1:446034/143‑1 (MQ=255) gACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCACTGGACTTCATCATATTGCTGT‑AATGCCTGCACCCGACCgg > 2:671490/1‑143 (MQ=255) aCATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAggt > 1:434275/1‑143 (MQ=255) tCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCACGTACGCCTGCCACGTCGCTTTCTGCGCGATGCCGCTGACCGTAATCGCACGTCACGCAATGCACTTCATCATGTTGTCGT‑AATGCCTGCACCAGACAgggggt > 2:221501/1‑143 (MQ=255) cAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCCGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATGGCTGT‑AATGCCTGCACCAGACAGGtggcgg > 2:50175/1‑143 (MQ=255) gCACCTTATGCGCGCGGGCCCCCCGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACTGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGaa < 2:363132/143‑1 (MQ=255) cTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGGGGAATccg > 1:1670/1‑142 (MQ=255) tATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTCCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTCGTGGacccctcg > 2:329766/1‑136 (MQ=255) gtGCGCCCGGGGCCCCAGGTTCAGCGCCAGGGCGCGTGCCACGTCGGTTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTGCATCATATTGTTGT‑AATGCCGGCACCAGACAGGTGGTGGAATCCTGAc < 2:382921/142‑1 (MQ=255) cgTGCCCCCAGTTTCAGCGCCAGTGCGCGCGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACt < 1:88193/143‑1 (MQ=255) gCCCTCCGTGTCAGCGCCAGTTCGCTTGCCACGTGAGGTCCTACTCGATGTCGCTGATCGTCATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGTCCTCCACTGaa < 2:319023/143‑1 (MQ=255) gCCCGCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGaa < 2:61041/143‑1 (MQ=255) gCCCCCAGTTTCAGAGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGGAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGaa < 1:155811/143‑1 (MQ=255) cccccAGTTCCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAac < 1:715211/143‑1 (MQ=255) ccccAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGACCGTCACGCAATGGACTTCATCACATTGTCGT‑ACTGCCTGCACCAGACAGGTCCTCGCATCCTGCCCCCCAccccacc > 2:188170/1‑137 (MQ=255) aGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAAc < 2:476604/143‑1 (MQ=255) tCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATACTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACgcac > 2:348781/1‑140 (MQ=255) aGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATAttgtt < 1:109568/85‑1 (MQ=255) aGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATAttgtt > 2:109568/1‑85 (MQ=255) tCGCGTGCCACGGCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCAt < 1:251455/143‑1 (MQ=255) cacgTGCCACGTCGTTTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACGTCATCATATTGTTGT‑AATGCCTGCACCGGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATa < 2:278211/141‑1 (MQ=255) gcgTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATCCACCACAGCAAATTGTTGT‑AATGCCTGCACCAGACAGCTGGTCAAACCCTGACCGCCACTGAACAAAACGACAGCACgatgcacaa > 2:62383/1‑135 (MQ=255) cgTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGa > 2:461403/1‑143 (MQ=255) cACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGACCGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACCCCACGACAGCCCGTTTCATCGATAATc > 2:519472/1‑143 (MQ=255) aCGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATcc > 1:692532/1‑143 (MQ=255) gTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGCGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTg > 1:677818/1‑143 (MQ=255) tCGATTTCTGCGCGATGCCGCTGACCGTAATCGAAGGTCACGCAAGGGACGTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTGTCATAGATAATCCTGc > 1:730248/1‑143 (MQ=255) tgtCTGCGCGATGCCGCTGACCGTAAGCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGa < 1:248811/141‑1 (MQ=255) tGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACCGGTGGTGGAATCCTGACCTCCACTGAACACAACGACCGCACGGTTCATAGATAATCCTGCTTgcccca > 1:347057/1‑138 (MQ=255) ctcgATGCCGCTGACCGTAATCTAACGTCACGCAATGGACTTCTTC‑TTTTCATGTCAACCCCTGCGCCGAACACCTGCTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATa < 2:322158/141‑1 (MQ=255) cgcgATGCCGCTGACCGTAATCGGACGTCACGCAATGGACTTCATCACATTGTCGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATa < 1:186132/143‑1 (MQ=255) cGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCATGCTTGAACAAGAACAgacaacacgtat > 1:330218/1‑132 (MQ=255) tCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AACGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAAT‑AAAGCGTTATGGTAACGCCTg < 2:96925/143‑1 (MQ=255) cGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATAAAAGCGTTATGGTAACGCCTg > 1:290079/1‑143 (MQ=255) aaCGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACAAGACAGGTGGTGGAATCCTGACGTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATAAAAGCGTCATGGTAACGCCTgac > 2:487223/1‑141 (MQ=255) aCGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATAAAAGCGTTATGGTAACGCCTGCGa < 1:278562/143‑1 (MQ=255) cGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATAAAAGCGTTATGGTAACGCCTGCGATTAAcc < 1:441346/143‑1 (MQ=255) | GTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTGT‑AATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATAAAAGCGTTATGGTAACGCCTGCGATTAACC > NC_000913/465324‑465599 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |