Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 3,951,734 Δ326 bp pseudogene (194‑519/663 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive, acetolactate synthase II, large subunit, cryptic, interrupted

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3951734 3952059 326 45 [0] [1] 45 ilvG pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive, acetolactate synthase II, large subunit, cryptic, interrupted

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 39517330 (0.000)42 (0.660) 37/268 0.8 100% pseudogene (193/663 nt) ilvG pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive, acetolactate synthase II, large subunit, cryptic, interrupted
?NC_000913 3952060 = 0 (0.000)pseudogene (520/663 nt) ilvG pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive, acetolactate synthase II, large subunit, cryptic, interrupted

CAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATC  >  NC_000913/3951922‑3952199
                                                                                                                                          |                                                                                                                                           
atcccggtgacgctatctacgcgccgtcgttgttaaaacaactgtcggatcgtaaacctgcggattgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCa                                                                                                                                         <  2:670823‑M2/5‑1 (MQ=255)
 tcccggtgacgctatctacgcgccgttgttgttaaaacaactgtcggatcgtaaacctgcggattgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAg                                                                                                                                        <  2:196722‑M2/6‑1 (MQ=255)
     ggtgacgctatctacgcgccgttgttgttaaaacaactgtcggatcgtaaacctgcggattgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccgggaaGACCGGGTTg                                                                                                                                    >  2:651964‑M2/134‑143 (MQ=255)
        gacgctatctacgcgccgttgttgttaaaacaactgtcggagcgtaaacctgcggattgcggcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAg                                                                                                                                 <  1:1119247‑M2/13‑1 (MQ=255)
          cgctatctacgcgccgctgttgttaagccaactgtcggatcgtaaacctgcggattgcgtcgtgacgacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCg                                                                                                                               <  1:1113766‑M2/15‑1 (MQ=255)
               tctacgcgccgttgttgttaaaacaactgtcggatcgtaaacctgcggattgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggcaaGACCAGGTTGAAGCGGCACt                                                                                                                          >  2:1045329‑M2/124‑143 (MQ=255)
                   cgcgccgttgttgtgaaaacaactgtcggatcgtgaaacttcgtatggcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGac                                                                                                                      <  1:1126647‑M2/24‑1 (MQ=255)
                      gccgttgttgttaaaacaactgtcggatcgtaaacctgcggattgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGCCGCGGCCCTCGAcccc                                                                                                                   >  2:916779‑M2/117‑143 (MQ=255)
                        cgttggtgttaaaacaactgtcggatcgtaaacctgcggattgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCAt                                                                                                                 <  2:462215‑M2/29‑1 (MQ=255)
                            gttgttaaaacaactgtcggatcgtaaacctgcggattgcgtcgtgaccacagatgtggcgccgcaccagatgtgggctgcgcagcacatcgcccacactcgcccagaaaGACCAGGTTGAAGCGGCACTGCACAACATGCTg                                                                                                             >  2:5064‑M2/111‑143 (MQ=255)
                                ttaagacagctgtcggatcgtaaacctgcggattgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccgcactcgcccggaaaGAGCGGGTTGAAGCTGCACTCGACACCATGCTGAACa                                                                                                         <  1:143091‑M2/37‑1 (MQ=255)
                                ttaaaacaactgtcggatcgtaaacctgcggattgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACa                                                                                                         >  1:515960‑M2/107‑143 (MQ=255)
                                ttaaaacaactgtcggatcgtaaacctgcggattgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTCGAAGCGGCACTCGACACCATGCTGACCa                                                                                                         >  1:364408‑M2/107‑143 (MQ=255)
                                ttaaaacaactgtcggatcgtaaacctgcggattgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGATGAAGCGGCACTCGACACCAGGCTGAACa                                                                                                         >  1:678638‑M2/107‑143 (MQ=255)
                                       aactgtcggatcgtaaacctgcggattgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATgg                                                                                                  <  2:691240‑M2/44‑1 (MQ=255)
                                       aactgtcggatcgtaaacctgcggattgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGAACCCATGCTGAACAGCGAggg                                                                                                  >  1:855606‑M2/100‑143 (MQ=255)
                                         ctgtcggatcgtacacctgcggcgtgcgtcgtgaccacggatgcggggcagcactatatgtgcgctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGc                                                                                                <  1:668048‑M2/46‑1 (MQ=255)
                                         cggtcggaccctcaacctgcggattgcgtcgtgaccacagatgtggggcagcaccagatgggggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGc                                                                                                <  2:35134‑M2/46‑1 (MQ=255)
                                           gtcggatcgtaaacctgcggattgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGGTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCa                                                                                              >  2:1084237‑M2/96‑143 (MQ=255)
                                            tcggatcgtaaacctgcggattgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacctcgcccacactcgcccggacaGACCCGGTTGAAGCGGCACTCGACGCCATGCTGAACAGTGATGCGCCAt                                                                                             >  2:729654‑M2/95‑143 (MQ=255)
                                                  cgtaaacctgcggattgcgtcgagccgacagaggtgggacctgaccatattcgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGc                                                                                       <  2:843183‑M2/55‑1 (MQ=255)
                                                   gtaaaccgccggattgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCt                                                                                      <  2:407068‑M2/56‑1 (MQ=255)
                                                    taaacctgcggattgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCtt                                                                                     >  2:202144‑M2/87‑143 (MQ=255)
                                                     aaacctgcggattgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTc                                                                                    <  2:450469‑M2/58‑1 (MQ=255)
                                                     aaacctgcggagtgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTc                                                                                    <  2:930878‑M2/58‑1 (MQ=255)
                                                         ctgcggattgcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGGACAGTGATGGGCCATACCTGCTGCATGt                                                                                >  2:208296‑M2/82‑143 (MQ=255)
                                                               gtggcgtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAAt                                                                          <  1:794296‑M2/68‑1 (MQ=255)
                                                                    gtcgtgaccacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATcgacg                                                                     >  2:651850‑M2/71‑143 (MQ=255)
                                                                          accacagatgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATCCCTGCTTCATGTCTCAATCGACGACCTTg                                                               >  2:355180‑M2/65‑143 (MQ=255)
                                                                           gcacagctgtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGAGCAGGTTGAAGCGGAAATCGTCAGCATGCTGAACAGTGATGGGCAATACCTGCTTCATGTCTCAATCGACGAACTTga                                                              <  1:247137‑M2/80‑1 (MQ=255)
                                                                               agatgtggggcagcacgaggtgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAATCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAAc                                                          <  1:928809‑M2/84‑1 (MQ=255)
                                                                                   gtggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCt                                                      >  2:1004260‑M2/56‑143 (MQ=255)
                                                                                     ggggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTgg                                                    <  2:565813‑M2/90‑1 (MQ=255)
                                                                                      gggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGc                                                   <  2:1142227‑M2/91‑1 (MQ=255)
                                                                                       ggcagcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACAAGGTTGAAGCGGCACGCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCCATCGACGAACTTGAGTACGTCTGGcc                                                  >  2:22149‑M2/52‑143 (MQ=255)
                                                                                           gcaccagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTg                                              <  2:192270‑M2/96‑1 (MQ=255)
                                                                                               cagatgtgggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACACTGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCCGGTgc                                          >  1:76987‑M2/44‑143 (MQ=255)
                                                                                                      gggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGCAGCGGCACGCGACACCATGCTGCACAGTGATGGGCCATACCTGCTCCATGTCTCAATCGACGCACTCGAGAACGTCTGGCCGCtgctgccgcgtcg                                   >  1:237988‑M2/37‑139 (MQ=255)
                                                                                                       ggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACCGCGATGGGCCATACCTGCTTCATGTCCCAATCGACGAACCTGAGCACGTCTGGCCGCTCGTGCCGCCCGgc                                  >  1:424831‑M2/36‑143 (MQ=255)
                                                                                                       ggctgcgcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGCGATGCGCCATACCTGCTTCATGTCTCAATCGACGAACTCGAGAACGTCCGGCcgctcgtgcccgccggc                                  >  1:35823‑M2/36‑136 (MQ=255)
                                                                                                             gcagcacatcgcccacactcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACCGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGGACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGt                            >  2:128305‑M2/30‑143 (MQ=255)
                                                                                                                  acatcgcccaccctcgcccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTc                       <  2:948593‑M2/119‑1 (MQ=255)
                                                                                                                                  cccGGAAAGACCAGGGTGAAGCGGCACTCGACACCATGCTGAACAGTGAGGGGCCATACCTGGTTCATGTCTCACTCGACGAACTTGAGGAAGACTGGCCGCTGGCGCCGACTGGGCCCAGCAAATGAGAGATGAGGGAGaaa       >  1:630298/1‑143 (MQ=255)
                                                                                                                                       aaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATc  <  2:515960‑M2/140‑1 (MQ=255)
                                                                                                                                       aaaGACCAGGTTGAACCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCTGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATc  <  2:687640‑M2/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATC  >  NC_000913/3951922‑3952199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.