Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,697,375 T→C Q222Q (CAA→CAG hdhA ← 7‑alpha‑hydroxysteroid dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,697,3750TC98.5% 204.4 / NA 65Q222Q (CAA→CAGhdhA7‑alpha‑hydroxysteroid dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (38/26);  minor base G (0/1);  total (38/27)
Fisher's exact test for biased strand distribution p-value = 4.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGAATATTTTTTTCACCCAGGT  >  NC_000913/1697237‑1697514
                                                                                                                                          |                                                                                                                                           
cccacacGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCg                                                                                                                                         <  2:555751/140‑1 (MQ=255)
acaacaCGCACGGTTGTTTACCGGTACGTTAGTGAATGAATTGAGCTCCTGTACCCCTCCACCGGGGACGGTCAGAACGTGTCCGCTTACCCAGCTCGCACAAGGCGCGCACAGGAACAGCGCTGCGTTAGCAATATACTGCg                                                                                                                                         >  1:57500/1‑143 (MQ=255)
 caacaCGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCgg                                                                                                                                        >  2:618384/1‑143 (MQ=255)
  gatctgcgcgGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCCCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGt                                                                                                                                       <  2:1018046/135‑1 (MQ=255)
    cacgcacgGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTg                                                                                                                                     <  2:517490/143‑1 (MQ=255)
     acgcacgGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTgg                                                                                                                                    <  1:973575/143‑1 (MQ=255)
        cacgGTTGTTTACCGGTCCGTTAGTGTATTAATGGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAACGGAACAGCGCTGCGTTAGCAGTCTCCTGCGGttagatc                                                                                                                                 >  1:1018046/1‑138 (MQ=255)
           ggTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTCCGGTTGGCCCAGa                                                                                                                              >  2:583415/1‑143 (MQ=255)
            gttgttTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGAc                                                                                                                             <  2:370292/143‑1 (MQ=255)
            gttgttTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGAc                                                                                                                             >  2:496804/1‑143 (MQ=255)
               gttTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTCCCCAGCCCGCAGCAGGCGAGCGAAAGAAAAGCGCTGCGATATCACTGTCCAGCGGTTGGCTCAGACGTc                                                                                                                          >  1:746114/1‑143 (MQ=255)
                   aCCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGAt                                                                                                                      >  2:1088376/1‑143 (MQ=255)
                   aCCCGTCCGTTAGTGTATTTATTGATCTCCTGTACCCCACCACCGGAGACGGTGAAAATATATCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGAt                                                                                                                      <  1:408075/143‑1 (MQ=255)
                          cGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGCTCGCCgtg                                                                                                               >  2:134855/1‑143 (MQ=255)
                           gTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGCCGCGCAAAGGCACAGCGATGCGTTAGCATTATCCTGCGGTTGGCCCAGACGTCGGATCGGCgcga                                                                                                              >  1:478055/1‑140 (MQ=255)
                            gtAGTGTATTAATTGATCTCTGGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGtgtg                                                                                                             <  1:967322/142‑1 (MQ=255)
                              aGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCt                                                                                                           <  2:461457/143‑1 (MQ=255)
                               gtgtagtaactactcTCCTGCACCCTACCACCGGGGCCGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTg                                                                                                          <  2:394630/129‑1 (MQ=255)
                                  tATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGTTTGGCCCAGACGTCTGATCGGCGTGTGCTGtct                                                                                                       >  2:519305/1‑141 (MQ=255)
                                  tATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTaa                                                                                                       >  2:835754/1‑143 (MQ=255)
                                          taGCGCCTGCCCCCCCCCCCCGGACACGGTGAGAATTTGTCCGCTTACCGAGCTCGCAGCAGTCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTTATCGGCGTGTGCTGTAACAtttttt                                                                                               <  1:776691/142‑1 (MQ=255)
                                          gaccgccTGTACCCCACCACCGGAGACGGTGAGAATTGGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAAGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACAtttttt                                                                                               <  2:504158/138‑1 (MQ=255)
                                            gcgccTGGACGCCACCACGGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACGGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGt                                                                                             <  2:883415/140‑1 (MQ=255)
                                              tCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGCCGTCTGGTCGGCGTCTGCTGTAGCATTTTTTGTTc                                                                                           >  2:760167/1‑143 (MQ=255)
                                               ccTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCATACCCAGCTCGCAGCAGGCGCGCAAAGGAACAGCACAGCGCTAGCAATAGCCTGCGGTTGGCCTAGACGTCTACTCACCGTGTGCTGGAACGTTTTTTgggca                                                                                          >  1:283998/1‑139 (MQ=255)
                                               ccTGTACCCCACCACCGGAGACGGCGAGAATTTGTCCGATTACCCAGCTCGCAGCAGGAGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGAGGCCTGATAGGCGCGGGCTGTAACAtcttcggtgcc                                                                                          >  1:1030635/1‑136 (MQ=255)
                                                cTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATGCTGCCGTTGGCCCAGAGTTCTCATAGGCGTGTGCTATAACATTTTTTGTTcga                                                                                         >  1:648919/1‑141 (MQ=255)
                                                 tGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAAt                                                                                        <  1:943693/143‑1 (MQ=255)
                                                  gTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAAtt                                                                                       >  2:433322/1‑143 (MQ=255)
                                                     ccccACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGGTCAATTTCt                                                                                    >  2:66492/1‑143 (MQ=255)
                                                      cccACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTg                                                                                   <  2:1093186/143‑1 (MQ=255)
                                                       tcacctcCGGAGACGGGGAGAATTTGTTCGTTTACCCTGCTCTCAGCAGGCGGGCAAAGGAACAGCGCTGCCTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTgg                                                                                  <  1:751469/142‑1 (MQ=255)
                                                       ccaccaCCGGAGACGGTGAGAATTTGTCCGCTTACCCCGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTgg                                                                                  <  2:1035443/143‑1 (MQ=255)
                                                        caccacCGGAGGCGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAAAAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGGGCTGTGCCAGTGTTTGTGCAATTTCTgga                                                                                 >  2:1045899/1‑142 (MQ=255)
                                                                gagaCGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACCTTTTTTGTTCAATTTCTGGTGTAATAAc                                                                         >  1:671458/1‑143 (MQ=255)
                                                                 agaCGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAAGATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACg                                                                        <  1:212835/143‑1 (MQ=255)
                                                                     ggTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGAtt                                                                    <  2:499517/143‑1 (MQ=255)
                                                                      gTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACACTTTTTGTTCAATTGCTGGTGTAATAACGGAttt                                                                   >  1:662535/1‑143 (MQ=255)
                                                                       tGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGAGTTc                                                                  >  1:60835/1‑143 (MQ=255)
                                                                          gaATCTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAgg                                                               <  2:569111/143‑1 (MQ=255)
                                                                           aaTTTGTCCGCTTACCCAGCGCGCCGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAggg                                                              <  2:53509/143‑1 (MQ=255)
                                                                             tttGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCCTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATACCGGATCTCAGGCCa                                                            >  1:201710/1‑143 (MQ=255)
                                                                                    gCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTCCAATTTCTCGTGTAATAACGGATTTCAGGGCATCGGTTa                                                     >  1:662456/1‑143 (MQ=255)
                                                                                    gCTCACCCCGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTa                                                     <  2:145663/143‑1 (MQ=255)
                                                                                       tACCCAGCTCGCAGCAGGTCGTCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAata                                                  <  2:427765/143‑1 (MQ=255)
                                                                                         cccAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGTCGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATAtt                                                <  1:936755/143‑1 (MQ=255)
                                                                                          tttgctcgcctgctgCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTg                                               >  2:99454/15‑143 (MQ=255)
                                                                                          tttgctcgcctgctgCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTg                                               >  1:728100/15‑143 (MQ=255)
                                                                                          ccAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTg                                               >  1:911329/1‑143 (MQ=255)
                                                                                            aGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGcc                                             >  1:2473/1‑143 (MQ=255)
                                                                                              cTCGCAGCAGGCGAGAAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGcccc                                           >  1:980040/1‑143 (MQ=255)
                                                                                                cGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCCTCGGTTAATATTGCCCCCg                                         >  1:986847/1‑143 (MQ=255)
                                                                                                   agcagGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGgcg                                      >  1:137280/1‑143 (MQ=255)
                                                                                                         cGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAAAATTTTTTGTTCCATTTCTGGTGTAATAACGGCATTCAGGTCTTCGGTTAGTATTGCCCCCGgcgggacgc                                >  2:306244/1‑137 (MQ=255)
                                                                                                          gAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGAGCATCGGTTAATATTGCCCACGGCGCAATGcc                               >  1:498245/1‑143 (MQ=255)
                                                                                                           aGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAACTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCa                              <  2:181048/143‑1 (MQ=255)
                                                                                                           aGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTATTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCa                              >  2:303736/1‑143 (MQ=255)
                                                                                                             cAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCAtt                            <  2:1142570/143‑1 (MQ=255)
                                                                                                                  gAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTAccc                       >  2:1061552/1‑143 (MQ=255)
                                                                                                                      agagcTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTATGCTGTAACATTTTTTGTTCAATTTATGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCCACGCCATTTACCCGAat                   >  1:1113387/4‑143 (MQ=255)
                                                                                                                      aGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCGGGGCGTCGGTTAATATTGCCCCCGGCGCGCTGCCATGTACCCGagt                   >  2:403129/1‑141 (MQ=255)
                                                                                                                      aGCGCGGCGTTAGCAATATCGTGCTGTTGGTACAGACGTCTGTTCGGCGTGTGCTGGAACATTTTTTTTTCAATTTCTTGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGTCCCCGGCGCAATGCCATTTACCCGAat                   <  1:1103988/143‑1 (MQ=255)
                                                                                                                          cTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTCTTCAATTTCTGGTGTAATAGCGGATTTCAGGGCATCGGTTGATATGGCCCCGGGCGGACTGGCATTTCCCCgcgtatgt               >  2:62634/1‑136 (MQ=255)
                                                                                                                            gCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGAATAttttt             <  2:728100/143‑1 (MQ=255)
                                                                                                                                       atCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGCGTTTCAGGGCCTCCGTTAATATTGCCCCCGGCGCAATGCCACCTACCCCAATATTTTCTTCACCCAgcg  >  1:336458/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                           
ACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGAATATTTTTTTCACCCAGGT  >  NC_000913/1697237‑1697514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: