breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 754,654 G→T intergenic (‑186/‑523) gltA ← / → sdhC citrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556
RA 1,158,626 G→A G253D (GGC→GAC)  ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
JC JC 2,413,482 IS5 (–) +4 bp coding (13‑16/1203 nt) ackA → acetate kinase A and propionate kinase 2
RA 2,722,202 T→G D750E (GAT→GAG pka → protein lysine acetyltransferase
MC JC 3,176,353 (GCA)4→3 coding (479‑481/828 nt) cpdA ← 3',5' cAMP phosphodiesterase
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,801,032 C→T G323E (GGA→GAA)  waaR ← lipopolysaccharide 1,2‑glucosyltransferase; UDP‑glucose:(glucosyl)LPS alpha‑1,2‑glucosyltransferase
JC 3,815,859 Δ82 bp [rph][rph] [rph], [rph]
MC JC 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,287,443 G→T intergenic (‑72/‑321) acs ← / → nrfA acetyl‑CoA synthetase/nitrite reductase, formate‑dependent, cytochrome
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 123048 127562 4515 10 [8] [9] 10 [aceE]–[aceF] [aceE],[aceF]
* * ÷ NC_000913 1293040 1312560 19521 36 [0] [0] 46 tdk–[yciB] tdk,insZ,insZ,insZ,adhE,ychE,oppA,oppB,oppC,oppD,oppF,yciU,clsA,yciY,kch,yciI,tonB,yciA,[yciB]
* * ÷ NC_000913 2066278–2066587 2066587 1–310 10 [8] [9] 11 [insH1] [insH1]
* * ÷ NC_000913 3423733–3424234 3424547–3424238 5–815 11 [9] [9] 12 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 257908 =NA (NA)35 (0.960) 33/260 0.2 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 1293039 0 (0.000)intergenic (‑117/‑488) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 = 258675NA (NA)46 (1.270) 39/260 0.1 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 1312561 = 0 (0.000)coding (330/540 nt) yciB IspA family inner membrane protein
* ? NC_000913 274620 =NA (NA)10 (0.300) 8/242 3.0 NA noncoding (530/1195 nt) IS5 repeat region
?NC_000913 274663 = NA (NA)noncoding (487/1195 nt) IS5 repeat region