Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 395,476 G→T S271Y (TCT→TAT)  ampH ← D‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase; penicillin‑binding protein; weak beta‑lactamase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913395,4760GT98.4% 396.5 / ‑2.3 126S271Y (TCT→TAT) ampHD‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase; penicillin‑binding protein; weak beta‑lactamase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (64/60);  total (65/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 126 total aligned reads displayed.

TCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGT  >  NC_000913/395336‑395621
                                                                                                                                            |                                                                                                                                                 
tCGGTGCCATATATACCAAACCTAAACCAAGCGCATCCGCTTTTCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCTCTTATAACTCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGAc                                                                                                                                              <  1:272514/146‑1 (MQ=255)
tCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGAc                                                                                                                                              <  8:225293/146‑1 (MQ=255)
   gggCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATAc                                                                                                                                        >  4:259844/1‑149 (MQ=255)
     gCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATActg                                                                                                                                      <  4:233459/149‑1 (MQ=255)
      ccATATATACCCAACCTAAACCAAGCGAATCCGCTTTTCCTGGGACATACATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGATGATAAAAATCATATGACAAATActgc                                                                                                                                     >  8:216484/1‑149 (MQ=255)
       cATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATActgct                                                                                                                                    >  7:277847/1‑149 (MQ=255)
        atatatACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCCACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATActgctg                                                                                                                                   >  1:258460/1‑149 (MQ=255)
        atatatACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATActgctg                                                                                                                                   >  5:52800/1‑149 (MQ=255)
        atatatACCCAACCTAAAACAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTATCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATActgctg                                                                                                                                   >  5:380267/1‑149 (MQ=255)
         tatataCCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACtgctgc                                                                                                                                  <  4:135046/149‑1 (MQ=255)
           tataCCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATActg                                                                                                                                      <  8:341994/143‑1 (MQ=255)
           tataCCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATActg                                                                                                                                      >  7:341994/1‑143 (MQ=255)
               cccAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACCTCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAg                                                                                                                            >  2:165635/1‑149 (MQ=255)
                ccAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAgc                                                                                                                           >  5:254485/1‑149 (MQ=255)
                ccAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAgc                                                                                                                           <  8:277847/149‑1 (MQ=255)
                ccAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAgc                                                                                                                           >  7:164692/1‑149 (MQ=255)
                  aaCCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCATTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAccgc                                                                                                                         <  8:131899/149‑1 (MQ=255)
                  aaCCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAgcg                                                                                                                          >  8:303823/1‑148 (MQ=255)
                          ccAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATc                                                                                                                 >  3:239581/1‑149 (MQ=255)
                             aGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATActgc                                                                                                                                     >  5:317088/1‑126 (MQ=255)
                             aGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGcc                                                                                                              <  6:166582/149‑1 (MQ=255)
                              gcgcATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCa                                                                                                             <  8:182718/149‑1 (MQ=255)
                               cgcATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCTCGCGCTGATAAATCAACGTCTGCATTCGATATGCCTGGTTACTGAGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATAATATCGCCAg                                                                                                            <  6:392739/149‑1 (MQ=255)
                               cgcATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAg                                                                                                            >  6:111065/1‑149 (MQ=255)
                               cgcATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAg                                                                                                            <  1:369398/149‑1 (MQ=255)
                               cgcATCCGATTTGCCGGGTACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACCTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGAAAAATACTGCTGCATCCAGCGCATCATATCGCCAg                                                                                                            <  7:188478/149‑1 (MQ=255)
                                     cGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTg                                                                                                       <  6:254485/148‑1 (MQ=255)
                                      gCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTgg                                                                                                      >  4:111733/1‑148 (MQ=255)
                                       atttGCCGTTGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGAGATCTGCCTTGTTACTGCGCTGATAAAAATCATATTACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGaa                                                                                                    <  6:87284/148‑1 (MQ=255)
                                        tttGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTACTGCATCCAGCGCATCATATCGCCAGGCGTGGaa                                                                                                    >  1:138713/1‑148 (MQ=255)
                                         ttGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCAAGCGcatc                                                                                                                      <  7:368252/129‑1 (MQ=255)
                                            ccGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCTCATCATATCGCCAGGCGTGGAATAGAc                                                                                               <  1:6032/149‑1 (MQ=255)
                                            ccGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGAc                                                                                               >  4:360086/1‑149 (MQ=255)
                                             cGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAg                                                                                                            >  8:388759/1‑135 (MQ=255)
                                              ggggACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGa                                                                                                     >  2:27353/1‑141 (MQ=255)
                                              ggggACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGa                                                                                                     <  1:27353/141‑1 (MQ=255)
                                                 gACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGAcgccgc                                                                                          <  8:164692/149‑1 (MQ=255)
                                                  aCATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGAcgccgcc                                                                                         >  1:56066/1‑149 (MQ=255)
                                                    aTCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATGCTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGAcgccgccgc                                                                                       >  1:94963/1‑149 (MQ=255)
                                                      ccATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGTCGCTg                                                                                     >  7:338073/1‑149 (MQ=255)
                                                      ccATGCCAATCACTTTCGTAAACTGCGCGAGCTGATAAATCAACGTCTGCATCCGATCTGCCTGTTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCATCGcatca                                                                                                                     >  5:401261/1‑117 (MQ=255)
                                                       cATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTTCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCAAGGCGTGGAATAGACGCCGCCGCTg                                                                                     <  5:162909/148‑1 (MQ=255)
                                                       cATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGc                                                                                    <  4:235500/149‑1 (MQ=255)
                                                       cATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGc                                                                                    >  5:214607/1‑149 (MQ=255)
                                                        aTGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGc                                                                                    >  3:347840/1‑148 (MQ=255)
                                                         tGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGcc                                                                                   <  6:340991/148‑1 (MQ=255)
                                                          gCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGAATCATATCGCAAGGCGTGGAATAGACGACGCCGCTGCCaa                                                                                 >  1:120225/1‑149 (MQ=255)
                                                             aaTCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTg                                                                              >  5:301992/1‑149 (MQ=255)
                                                              aTCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACaaa                                                                                                                                           >  3:102422/1‑87 (MQ=255)
                                                              aTCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACaaa                                                                                                                                           <  4:102422/87‑1 (MQ=255)
                                                               tCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTgcc                                                                            <  6:52800/149‑1 (MQ=255)
                                                                cACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTgccg                                                                           >  1:100831/1‑149 (MQ=255)
                                                                 aCTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTgccgc                                                                          >  1:128535/1‑149 (MQ=255)
                                                                   tttCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGCCTCCGCCGCTGCCAATTgccgc                                                                          >  6:158064/1‑147 (MQ=255)
                                                                     tCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGc                                                                      >  8:65478/1‑149 (MQ=255)
                                                                      cGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTACTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGc                                                                      <  2:138713/148‑1 (MQ=255)
                                                                       gTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCgt                                                                    >  2:410803/1‑149 (MQ=255)
                                                                            cTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTAt                                                               <  5:52177/149‑1 (MQ=255)
                                                                            cTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTAt                                                               >  2:293367/1‑149 (MQ=255)
                                                                             tGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGAc                                                                                                                                              <  1:87869/69‑1 (MQ=255)
                                                                             tGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTAtt                                                              >  4:348600/1‑149 (MQ=255)
                                                                               cgcgcgcTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTgccgc                                                                          >  7:323823/1‑135 (MQ=255)
                                                                               cgcgcgcTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTAtt                                                              <  2:56066/147‑1 (MQ=255)
                                                                               cgcgcgcTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTg                                                             >  3:160621/1‑148 (MQ=255)
                                                                               cgcgcgcTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGc                                                            <  3:360086/149‑1 (MQ=255)
                                                                                    gcTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGa                                                       >  8:293360/1‑149 (MQ=255)
                                                                                    gcTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGa                                                       >  8:353610/1‑149 (MQ=255)
                                                                                     cTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGAc                                                      <  1:410803/149‑1 (MQ=255)
                                                                                     cTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGAc                                                      <  2:128535/149‑1 (MQ=255)
                                                                                      tGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACt                                                     >  8:41713/1‑149 (MQ=255)
                                                                                       gATAAAACAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATCACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATACACGCCGCCCCTGCCAATTGTCGCCAGCGTCTTATTGCACGGACTg                                                    >  6:108305/1‑149 (MQ=255)
                                                                                        aTAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCTGCGCATCATTTCGCCAGGCGTGGAATATACGCCGACGCTGCAAATTGCCGACAGCGTGTTATTGAACGGACt                                                     <  6:380267/147‑1 (MQ=255)
                                                                                        aTAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTgg                                                   <  6:301992/149‑1 (MQ=255)
                                                                                           aaTCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCa                                                 <  4:347840/148‑1 (MQ=255)
                                                                                            aTCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGTACTGGCAcc                                               >  3:37110/1‑149 (MQ=255)
                                                                                              cAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTgg                                                   <  2:53159/143‑1 (MQ=255)
                                                                                                   tCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCTCTCa                                        <  7:16625/149‑1 (MQ=255)
                                                                                                       cATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCaa                                    <  2:258460/149‑1 (MQ=255)
                                                                                                       cATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATGCTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGc                                      <  2:94963/147‑1 (MQ=255)
                                                                                                        aTGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGTACTGGCACCGCGCTCAGCAAc                                   <  4:37110/149‑1 (MQ=255)
                                                                                                        aTGCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCAAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAAc                                   >  8:118664/1‑149 (MQ=255)
                                                                                                          gCGATCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCa                                 >  3:765/1‑149 (MQ=255)
                                                                                                             aTCTGCCTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCAtt                               >  2:158435/1‑148 (MQ=255)
                                                                                                                  ccTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAAcgg                         <  3:111733/149‑1 (MQ=255)
                                                                                                                  ccTGGTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAAcgg                         >  8:363949/1‑149 (MQ=255)
                                                                                                                      gTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGTCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAAcggcg                       <  8:338073/147‑1 (MQ=255)
                                                                                                                      gTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAAcggcgg                      <  1:376830/148‑1 (MQ=255)
                                                                                                                      gTTACTGCGCTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAAcggcgg                      <  7:41713/148‑1 (MQ=255)
                                                                                                                             cgcTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGAt               <  6:214607/148‑1 (MQ=255)
                                                                                                                              gcTGATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATcc             <  7:65478/149‑1 (MQ=255)
                                                                                                                                 gATAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGCTCCGGt          >  6:293621/1‑149 (MQ=255)
                                                                                                                                  aTAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCTCCGCTGCCAATTGCCGCCATCGTGTTATTGCACGGACTGGCAcc                                               <  1:339070/111‑1 (MQ=255)
                                                                                                                                  aTAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCgg           <  7:293360/147‑1 (MQ=255)
                                                                                                                                  aTAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTg         <  5:102406/149‑1 (MQ=255)
                                                                                                                                  aTAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCAGCCGCTGCCAATTGCCGCAAGCGTGTTATTGCACGGACTGTCAcc                                               >  2:339070/1‑111 (MQ=255)
                                                                                                                                   tAAAAATCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGt          >  8:284069/1‑147 (MQ=255)
                                                                                                                                     aaaaTCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAgg      <  6:363721/149‑1 (MQ=255)
                                                                                                                                      aaaTCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAggg     >  4:186554/1‑149 (MQ=255)
                                                                                                                                       aaTCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAgggg    >  1:273569/1‑149 (MQ=255)
                                                                                                                                       aaTCATATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAgggg    <  4:765/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
TCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGT  >  NC_000913/395336‑395621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: