| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,775,311 | G→T | 5.1% | Q296H (CAG→CAT) | mtlD → | mannitol‑1‑phosphate dehydrogenase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,775,311 | 0 | G | T | 5.1% | 285.5 / 3.8 | 79 | Q296H (CAG→CAT) | mtlD | mannitol‑1‑phosphate dehydrogenase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (37/38); new base T (2/2); total (39/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01 | |||||||||||
GATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAAC > NC_000913/3775176‑3775456 | gATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTaaa < 5:99256/148‑1 (MQ=255) gaagaaAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTg < 8:156853/147‑1 (MQ=255) aaaGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGc > 1:239079/1‑148 (MQ=255) aaaGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGc > 5:159009/1‑148 (MQ=255) aaGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCt < 6:144714/148‑1 (MQ=255) aTTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCATCCACTGCGTaa < 4:173345/126‑1 (MQ=255) aTTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCATCCACTGCGTaa < 4:292768/126‑1 (MQ=255) aTTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCATCCACTGCGTaa > 3:292768/1‑126 (MQ=255) aTTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCATCCACTGCGTaa > 3:173345/1‑126 (MQ=255) aTTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTc > 3:72185/1‑146 (MQ=255) aTTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTc < 4:72185/146‑1 (MQ=255) aTTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCt > 7:73899/1‑147 (MQ=255) aTTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCt > 1:259506/1‑147 (MQ=255) aTTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCt > 7:76853/1‑147 (MQ=255) ttGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGa < 4:65270/148‑1 (MQ=255) ttGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGa > 3:65270/1‑148 (MQ=255) ttGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCAGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTg > 2:353439/1‑147 (MQ=255) tGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTaa > 2:344828/1‑124 (MQ=255) tGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTaa < 1:344828/124‑1 (MQ=255) aaGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGa > 1:165263/1‑124 (MQ=255) aaGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGa < 2:165263/124‑1 (MQ=255) aaGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGc > 8:363837/1‑148 (MQ=255) aGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGcc < 8:76853/148‑1 (MQ=255) aGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGc > 8:185671/1‑147 (MQ=255) tACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGcc < 3:221636/104‑1 (MQ=255) tACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGcc > 4:221636/1‑104 (MQ=255) aCGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCt > 1:304487/1‑148 (MQ=255) cGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGc < 7:185671/146‑1 (MQ=255) cTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGt < 2:259506/148‑1 (MQ=255) ttGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGt > 1:164076/1‑113 (MQ=255) ttGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGt < 2:164076/113‑1 (MQ=255) gACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACg < 2:304487/147‑1 (MQ=255) gCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCgg < 8:5097/140‑1 (MQ=255) gCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCgg > 7:5097/1‑140 (MQ=255) gcggcgTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGcc < 8:321675/119‑1 (MQ=255) gcggcgTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGcc > 7:321675/1‑119 (MQ=255) gcggcgTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGc > 8:175219/1‑148 (MQ=255) ggcgTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTc > 7:56760/1‑124 (MQ=255) ggcgTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTc < 8:56760/124‑1 (MQ=255) cgTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGt < 3:136649/125‑1 (MQ=255) cgTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGt > 4:136649/1‑125 (MQ=255) aCATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATa > 8:241768/1‑147 (MQ=255) aCATCCAGAAAATTCTCGGACGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATa > 6:240639/1‑147 (MQ=255) aTCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATaaaa < 1:353439/148‑1 (MQ=255) ccAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGcc > 4:126581/1‑108 (MQ=255) ccAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGcc < 3:126581/108‑1 (MQ=255) cAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCa < 6:312816/103‑1 (MQ=255) cAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCa > 5:312816/1‑103 (MQ=255) aaaaTTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGt < 2:149574/129‑1 (MQ=255) aaaaTTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGt > 1:149574/1‑129 (MQ=255) aTTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGAtt > 3:243320/1‑147 (MQ=255) ttCTCGGCCGATTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGa < 6:52922/148‑1 (MQ=255) ggCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAgg < 7:175219/146‑1 (MQ=255) ggCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTa < 8:73899/148‑1 (MQ=255) ttttGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGa < 4:190069/138‑1 (MQ=255) ttttGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGa > 3:190069/1‑138 (MQ=255) aTCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATaa < 5:209390/112‑1 (MQ=255) aTCTGAAAGATGAAGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATaa > 6:209390/1‑112 (MQ=255) cTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCa > 4:13934/1‑138 (MQ=255) cTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCa < 3:13934/138‑1 (MQ=255) cTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTcc > 8:40669/1‑148 (MQ=255) aaaGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCa < 7:241768/148‑1 (MQ=255) gatgatGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTg < 7:363837/148‑1 (MQ=255) tgatgTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTg > 6:99771/1‑146 (MQ=255) gatgTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTg > 8:29664/1‑145 (MQ=255) gatgTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTg > 5:36875/1‑145 (MQ=255) atgTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAg < 4:243320/147‑1 (MQ=255) tAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAgatg < 5:99771/147‑1 (MQ=255) cgTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAg < 7:40669/148‑1 (MQ=255) ccGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAg > 6:155769/1‑131 (MQ=255) ccGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAg < 5:155769/131‑1 (MQ=255) ccGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAg < 6:36875/148‑1 (MQ=255) ccGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAg > 3:266614/1‑148 (MQ=255) tCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGaa < 5:240639/148‑1 (MQ=255) cAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTg < 6:387882/106‑1 (MQ=255) cAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTg > 5:387882/1‑106 (MQ=255) cAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGa > 6:245573/1‑146 (MQ=255) cAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGa < 5:245573/146‑1 (MQ=255) aGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAAc < 4:266614/147‑1 (MQ=255) gCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAgg < 8:101531/143‑1 (MQ=255) gCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAgg > 7:101531/1‑143 (MQ=255) | GATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTGCAATGCACTTCCGCAGTGAAGATGATCCGCAGGCTCAGGAAC > NC_000913/3775176‑3775456 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |