Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,926,829 C→T 5.3% V59I (GTC→ATC)  asnC ← transcriptional activator of asnA; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,926,8290CT 5.3% 251.7 / 2.4 75V59I (GTC→ATC) asnCtranscriptional activator of asnA; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (33/38);  new base T (2/2);  total (35/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.16e-01

CTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGT  >  NC_000913/3926687‑3926975
                                                                                                                                              |                                                                                                                                                  
atGTAGTGGCAGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGACGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCaa                                                                                                                                               <  8:167330/147‑1 (MQ=255)
 tGTAGTGGACGGTTGTGTAATAGGCTTCTGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGTAAGGGTAGTCTTTGGCGCTCTTTATTATAATGCCGATAAAGCAGTCTACGTGATAACAGAGCTGATTCGGTCTGACATCatt                                                                                                                                              <  2:106644/148‑3 (MQ=255)
     gTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATAcgc                                                                                                                                          <  8:360473/148‑1 (MQ=255)
       ggCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACgcgc                                                                                                                                        >  2:266486/1‑148 (MQ=255)
         ccGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGcc                                                                                                                                       <  5:342526/147‑1 (MQ=255)
               gtgtAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTa                                                                                                                                 <  1:266486/147‑1 (MQ=255)
                tgtAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAAt                                                                                                                                              >  5:13864/1‑133 (MQ=255)
                tgtAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAAt                                                                                                                                              <  6:13864/133‑1 (MQ=255)
                      aGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTa                                                                                                                                 >  3:232052/1‑140 (MQ=255)
                      aGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTa                                                                                                                                 <  4:232052/140‑1 (MQ=255)
                        gCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCATTACGCGCCCCGGTAATGATCCCCg                                                                                                                       <  1:287796/148‑1 (MQ=255)
                                taCTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCtt                                                                                                                >  8:392131/2‑147 (MQ=255)
                                gaattCATCAAGGCTTTCCAGCTTTGCCAGCTCGGAAGGGTAGTCTTTTGCGCTCTTTAATATAATCCCGATAAAGAAGCCTACGTCATAACCGAGCTGATTCGGGATGACATCAATACGAGCCCCGGTAATTATCCCAGCATGCtt                                                                                                                >  3:299001/4‑147 (MQ=255)
                                gACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATAcgc                                                                                                                                          <  2:345468/121‑1 (MQ=255)
                                gACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATAcgc                                                                                                                                          >  1:345468/1‑121 (MQ=255)
                                       tcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTtc                                                                                                         <  3:21515/147‑1 (MQ=255)
                                       tcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTtc                                                                                                         >  4:21515/1‑147 (MQ=255)
                                        caAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGGCTTTGGCGCTCTTTAATATAATGCCGATATAGCAGCCTACGTCATAACCGAGCTGGTTCGGGCTGACATCAATACGCGCCGCGGTaa                                                                                                                                <  7:306969/123‑1 (MQ=255)
                                         aaGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCAGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACCCGCCCCGGTAAt                                                                                                                               >  8:306969/1‑123 (MQ=255)
                                            gCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTAGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACt                                                                                                   <  6:147892/148‑1 (MQ=255)
                                                tCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGa                                                                                                <  2:376926/147‑1 (MQ=255)
                                                              cgcgGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTtct                                                                                                        >  6:274776/1‑125 (MQ=255)
                                                              cgcgGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTtct                                                                                                        <  5:274776/125‑1 (MQ=255)
                                                                         aGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGc                                                                       >  5:140759/1‑147 (MQ=255)
                                                                          gTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCgg                                                                               >  8:360659/1‑138 (MQ=255)
                                                                          gTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCgg                                                                               <  7:360659/138‑1 (MQ=255)
                                                                           tCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTtc                                                                                                         <  4:136033/111‑1 (MQ=255)
                                                                           tCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTtc                                                                                                         >  3:136033/1‑111 (MQ=255)
                                                                            cTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCaaa                                                                   >  8:48140/1‑148 (MQ=255)
                                                                            cTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCaaa                                                                   >  6:38928/1‑148 (MQ=255)
                                                                             tttGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAAt                                                                  >  6:22247/1‑148 (MQ=255)
                                                                               tGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTctct                                                                                                      >  8:302597/1‑110 (MQ=255)
                                                                               tGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTctct                                                                                                      <  7:302597/110‑1 (MQ=255)
                                                                                 gcgcTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGtt                                                              <  5:22247/148‑1 (MQ=255)
                                                                                        ttAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTTTACTCGAACGTGAATCGTCCCCgg                                                                               >  4:54191/1‑124 (MQ=255)
                                                                                        ttAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTTTACTCGAACGTGAATCGTCCCCgg                                                                               <  3:54191/124‑1 (MQ=255)
                                                                                        ttAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAg                                                       >  3:190488/1‑148 (MQ=255)
                                                                                        ttAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAg                                                       >  8:281579/1‑148 (MQ=255)
                                                                                         tAATATAATTCCGATATAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGcc                                                         <  4:299001/145‑1 (MQ=255)
                                                                                             ataATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGATATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACg                                                                                             <  8:7053/105‑1 (MQ=255)
                                                                                             ataATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGATATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACg                                                                                             >  7:7053/1‑105 (MQ=255)
                                                                                             ataATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGATATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACg                                                                                             >  7:12863/1‑105 (MQ=255)
                                                                                             ataATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGATATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACg                                                                                             <  8:12863/105‑1 (MQ=255)
                                                                                             ataATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCgg                                                  >  5:29143/1‑148 (MQ=255)
                                                                                             ataATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTCAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCgg                                                  >  1:192208/1‑148 (MQ=255)
                                                                                               aaTTCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCgg                                                                               <  3:224736/117‑1 (MQ=255)
                                                                                               aaTGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCgg                                                                               >  4:224736/1‑117 (MQ=255)
                                                                                                aTGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCg                                                >  1:371496/1‑147 (MQ=255)
                                                                                                aTGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCg                                                >  3:227058/1‑147 (MQ=255)
                                                                                                  gCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGcc                                                         <  2:46541/136‑1 (MQ=255)
                                                                                                  gCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGcc                                                         >  1:46541/1‑136 (MQ=255)
                                                                                                     gATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCg                                          >  8:367645/1‑148 (MQ=255)
                                                                                                     gATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCg                                          <  2:371496/148‑1 (MQ=255)
                                                                                                     gATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCg                                          <  4:227058/148‑1 (MQ=255)
                                                                                                          agcagcCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTg                                                                           >  8:371805/1‑110 (MQ=255)
                                                                                                          agcagcCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTg                                                                           <  7:371805/110‑1 (MQ=255)
                                                                                                            cagcCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTgcgcg                                   <  4:190488/148‑1 (MQ=255)
                                                                                                             agcCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTgcgcgc                                  <  7:367645/148‑1 (MQ=255)
                                                                                                             agcCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTgcgcgc                                  <  7:392131/148‑1 (MQ=255)
                                                                                                             agcCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTgcgcgc                                  <  2:120074/148‑1 (MQ=255)
                                                                                                             agcCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTgcgcgc                                  <  6:29143/148‑1 (MQ=255)
                                                                                                                   cGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGT‑‑CCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTgc                                      >  7:374662/1‑136 (MQ=255)
                                                                                                                   cGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGT‑‑CCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTgc                                      <  8:374662/136‑1 (MQ=255)
                                                                                                                    gTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTgcgcgc                                  <  8:177830/141‑1 (MQ=255)
                                                                                                                    gTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTgcgcgc                                  >  7:177830/1‑141 (MQ=255)
                                                                                                                       aTAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCAt                         <  7:281579/147‑1 (MQ=255)
                                                                                                                       aTAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCAt                         <  3:130215/147‑1 (MQ=255)
                                                                                                                        tAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTa                       <  6:140759/148‑1 (MQ=255)
                                                                                                                              aGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCtt                 >  3:80545/1‑148 (MQ=255)
                                                                                                                                   ctTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAgg            <  1:32791/148‑1 (MQ=255)
                                                                                                                                   ctTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAgg            <  7:48140/148‑1 (MQ=255)
                                                                                                                                   ctTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAgg            <  5:38928/148‑1 (MQ=255)
                                                                                                                                   ctTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAg             >  2:32791/1‑147 (MQ=255)
                                                                                                                                             aCATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGt  >  5:323740/1‑148 (MQ=255)
                                                                                                                                             aCATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGt  >  3:335551/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
CTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGT  >  NC_000913/3926687‑3926975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: