Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 467,238 G→T 5.1% T25T (ACC→ACA ybaE ← putative ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913467,2380GT 5.1% 276.2 / 2.7 78T25T (ACC→ACAybaEputative ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (39/35);  new base T (2/2);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

GACCAGAAAGCGTAATTGTCCGCGCTTTCCGCGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACC  >  NC_000913/467100‑467382
                                                                                                                                          |                                                                                                                                                
gACCAGAAAGCGTAATTGTCCGCGCTTTCCGCGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGtt                                                                                                                                         >  3:214954/1‑148 (MQ=255)
gACCAGAAAGCGTAATTGTCCGCGCTTTCCGCGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGtt                                                                                                                                         >  5:241525/1‑148 (MQ=255)
       aaGCGTAATTGTCCGCGCTTTCCGCGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCt                                                                                                                                  >  6:104259/1‑148 (MQ=255)
                  tCCGCGCTTTCCGCGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGaa                                                                                                                       <  6:134305/148‑1 (MQ=255)
                  tCCGCGCTTTCCGCGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGa                                                                                                                        <  4:214954/147‑1 (MQ=255)
                  tCCGCGCTTTCCGCGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGa                                                                                                                        <  6:241525/147‑1 (MQ=255)
                  tCCGCGCTTTCCGCGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGa                                                                                                                        <  5:104259/147‑1 (MQ=255)
                  tCCGCGCTTTCCGCGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGa                                                                                                                        <  8:63919/147‑1 (MQ=255)
                    cgcgCTTTCCGCGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAg                                                                                                                      <  7:295630/147‑1 (MQ=255)
                            ccGCGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTGCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCcac                                                                                                              <  5:254283/147‑1 (MQ=255)
                                        tGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGc                                                                                                                                   <  2:169114/114‑1 (MQ=255)
                                        tGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGc                                                                                                                                   >  1:169114/1‑114 (MQ=255)
                                                   cTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATAc                                                                                                  >  4:209333/1‑136 (MQ=255)
                                                   cTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATAc                                                                                                  <  3:209333/136‑1 (MQ=255)
                                                    tCCCGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTg                                                                                                      <  8:209093/131‑1 (MQ=255)
                                                    tCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTg                                                                                                      >  7:209093/1‑131 (MQ=255)
                                                    tCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGAt                                                                                                    <  3:160427/133‑1 (MQ=255)
                                                    tCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGAt                                                                                                    >  4:160427/1‑133 (MQ=255)
                                                     ccAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTg                                                                                                      <  5:36414/130‑1 (MQ=255)
                                                     ccAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTg                                                                                                      >  6:36414/1‑130 (MQ=255)
                                                     ccAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTg                                                                                                      >  7:13590/1‑130 (MQ=255)
                                                     ccAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTg                                                                                                      <  8:13590/130‑1 (MQ=255)
                                                     ccAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGtt                                                                                    >  4:250743/1‑148 (MQ=255)
                                                     ccAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGtt                                                                                    >  8:343385/1‑148 (MQ=255)
                                                     ccAGCCATCCCGCCTCCTGAGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGCCGGTTTGCGGCTTTCCGGCGCAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGtt                                                                                    >  3:24862/1‑148 (MQ=255)
                                                       aGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGtt                                                                                    >  7:137748/1‑146 (MQ=255)
                                                             cccGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAgg                                                                                                                     >  1:13108/1‑107 (MQ=255)
                                                             cccGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAgg                                                                                                                     <  2:13108/107‑1 (MQ=255)
                                                             cccGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTg                                                                                   <  4:336658/141‑1 (MQ=255)
                                                             cccGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTg                                                                                   >  3:336658/1‑141 (MQ=255)
                                                             cccGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATc                                                                            >  4:169169/1‑148 (MQ=255)
                                                               cGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTg                                                                                                      <  3:321691/120‑1 (MQ=255)
                                                               cGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTg                                                                                                      >  4:321691/1‑120 (MQ=255)
                                                               cGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAAGCg                                                                           >  4:107989/1‑147 (MQ=255)
                                                                gCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCa                                                                         >  4:356153/1‑148 (MQ=255)
                                                                   tcctGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACTGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGc                                                                                <  1:319950/138‑1 (MQ=255)
                                                                   tcctGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACTGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGc                                                                                >  2:319950/1‑138 (MQ=255)
                                                                    cctGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCatat                                                                      >  5:272575/1‑147 (MQ=255)
                                                                             gACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATa                                                                                                   >  5:139722/1‑109 (MQ=255)
                                                                             gACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATa                                                                                                   <  6:139722/109‑1 (MQ=255)
                                                                             gACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGaa                                                            >  5:142964/1‑148 (MQ=255)
                                                                              aCGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCAACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGaa                                                            >  4:146804/1‑147 (MQ=255)
                                                                                    cAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTg                                                                                                      <  8:366116/99‑1 (MQ=255)
                                                                                    cAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTg                                                                                                      >  7:366116/1‑99 (MQ=255)
                                                                                    cAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAatat                                                      >  3:285944/1‑147 (MQ=255)
                                                                                        gTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATattatt                                                  >  3:273665/1‑147 (MQ=255)
                                                                                         tACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTAttt                                                 >  2:159328/1‑147 (MQ=255)
                                                                                          aCGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACTGTGACGTTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGc                                                                          <  3:130212/121‑1 (MQ=255)
                                                                                          aCGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACTGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGc                                                                          >  4:130212/1‑121 (MQ=255)
                                                                                          aCGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTaa                                               >  5:379133/1‑148 (MQ=255)
                                                                                            gAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAAc                                              >  4:292691/1‑147 (MQ=255)
                                                                                               cATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTAtt                                          <  3:146804/148‑1 (MQ=255)
                                                                                                 tGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCa                                        <  8:137748/148‑1 (MQ=255)
                                                                                                  ggCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTc                                         >  7:326187/1‑146 (MQ=255)
                                                                                                  ggCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTc                                         <  8:326187/146‑1 (MQ=255)
                                                                                                   gCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACt                                      <  4:273665/148‑1 (MQ=255)
                                                                                                    cGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTa                                     <  6:379133/148‑1 (MQ=255)
                                                                                                       tCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTa                                  >  6:279629/1‑148 (MQ=255)
                                                                                                        cGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTa                                  <  6:142964/147‑1 (MQ=255)
                                                                                                          cTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACtt                               <  6:272575/148‑1 (MQ=255)
                                                                                                           tGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTc                              >  4:127358/1‑148 (MQ=255)
                                                                                                           tGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTc                              >  6:222401/1‑148 (MQ=255)
                                                                                                             cAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAAtatt                                                     >  5:276318/1‑123 (MQ=255)
                                                                                                             cAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAAtatt                                                     <  6:276318/123‑1 (MQ=255)
                                                                                                                aaaCAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGtat                         <  7:343385/148‑1 (MQ=255)
                                                                                                                 aaCAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGtata                        >  7:13892/1‑148 (MQ=255)
                                                                                                                      cGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCa                   <  4:24862/148‑1 (MQ=255)
                                                                                                                       gTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAg                  <  3:127358/148‑1 (MQ=255)
                                                                                                                           ggCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTg               <  4:285944/147‑1 (MQ=255)
                                                                                                                           ggCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTg               <  3:356153/147‑1 (MQ=255)
                                                                                                                            gCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGAt             <  3:169169/148‑1 (MQ=255)
                                                                                                                            gCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGAt             <  5:222401/148‑1 (MQ=255)
                                                                                                                             ccAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATa            <  3:250743/148‑1 (MQ=255)
                                                                                                                                gTTCGCTGTCGGTGAAGGTTTGCGTCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTTGTTAAGACGGTTGAGCAATCGATTTTAATCCCGGAACAATATTATTTAATTATTGACTATTACTTGTGtatattt                     <  6:122209/136‑3 (MQ=255)
                                                                                                                                 ttCGCTGACGGTGACGGTTTGCGGCTTTCCGGCCGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATAATTTAACTATTCACTATTACTTCCGTATATATc                    >  5:122209/1‑136 (MQ=255)
                                                                                                                                  tCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCaa       >  2:135002/1‑148 (MQ=255)
                                                                                                                                  tCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATACTTCCGTATATATCAGGTGATACTCAAt      >  3:266298/1‑148 (MQ=255)
                                                                                                                                  tCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGGTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCat       >  4:15270/1‑147 (MQ=255)
                                                                                                                                       gacggtgacggtTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCAc   >  8:94943/1‑147 (MQ=255)
                                                                                                                                        acggtgacggtTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCAcc  <  8:13892/147‑1 (MQ=255)
                                                                                                                                          ggtgacggtTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTg               >  2:14840/1‑132 (MQ=255)
                                                                                                                                          ggtgacggtTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTg               <  1:14840/132‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                
GACCAGAAAGCGTAATTGTCCGCGCTTTCCGCGTCCTGACTGCGCCTGCCACTCCAGCCATCCCGCCTCCTGTGCCTGACGCAACAGCGTACGAACATGGCGTTCGCTGCAAAAACAGCGTTCGGCCAGTTCGCTGACGGTGACGGTTTGCGGCTTTCCGGCGGAAGGTTGCCACAGACGTTGATACTGGTTAAGACGGTTGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACC  >  NC_000913/467100‑467382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: