Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,626,708	A→G	6.3%	E266G (GAG→GGG)	radA →	DNA repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,626,708	0	A	G	6.3%	195.6 / 3.7	63	E266G (GAG→GGG)	radA	DNA repair protein
Reads supporting (aligned to +/- strand):  ref base A (28/31);  new base G (2/2);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

CACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTC  >  NC_000913/4626569‑4626854                                                                                                                                            |                                                                                                                                                   cACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCg                                                                                                                                            <  6:301594/148‑1 (MQ=255)    gTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCt                                                                                                                                                 >  8:267318/1‑140 (MQ=255)    gTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCt                                                                                                                                                 <  7:267318/140‑1 (MQ=255)    gTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCt                                                                                                                                         >  2:322392/1‑148 (MQ=255)        ccAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTcgc                                                                                                                                     >  4:214974/1‑148 (MQ=255)         cAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCCTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTcgc                                                                                                                                     >  3:1008/1‑147 (MQ=255)         cAAAGATGGTTCGCTGGCTGCCCCGAAAGTGCTCGAACACTGTATCGACTGTTCGGTGCTTATGGATGCCGATGCCGACTCCCGTATTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGAGAATGAGCTCCGCGTCTAcgc                                                                                                                                     >  4:339249/1‑147 (MQ=255)         cAAAGACGGTTCGCGGGCTGGCCCGAAAGGGCTGGAACACTGTATCGACTGTGCGGTGCTGTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTcgcg                                                                                                                                    <  8:212283/148‑1 (MQ=255)                 gTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCg                                                                                                                             >  1:212573/1‑147 (MQ=255)                  ttCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATGGACTGTTCGGTGCTTTTGGATGGCGATGCCGAGTCCCGTTTTCGCTCCTTGCGCAGCCATTAAAACCGCTTCGGCGCGGTGATTGAGCTGGGCGTGTTCGCGATGACCGaa                                                                                                                           <  3:339249/148‑1 (MQ=255)                    cGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAAc                                                                                                                          <  8:5253/147‑1 (MQ=255)                    cGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAAc                                                                                                                          <  1:322392/147‑1 (MQ=255)                     gctggctgGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATg                                                                                                                                 <  1:124119/139‑1 (MQ=255)                     gctggctgGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATg                                                                                                                                 >  2:124119/1‑139 (MQ=255)                                  aaaGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAg                                                                                                           >  8:324895/1‑148 (MQ=255)                                      tGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGGGAATGAGCTGCGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAg                                                                                                       >  5:359774/1‑148 (MQ=255)                                          ggAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAAc                                                                                                   >  1:318115/1‑148 (MQ=255)                                            aaCACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAAccc                                                                                                 <  2:182435/148‑1 (MQ=255)                                                  gTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCgg                                                                                            >  6:304039/1‑147 (MQ=255)                                                    aTCGACTGTTCGGTGCTTTTGGATGGCGATTCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCa                                                                                          <  6:54558/147‑1 (MQ=255)                                                           gTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCt                                                                                   >  1:323047/1‑147 (MQ=255)                                                           gTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGCCAATTTTCt                                                                                   >  2:322518/1‑147 (MQ=255)                                                             tCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCtt                                                                                  >  5:76435/1‑146 (MQ=255)                                                             tCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCtt                                                                                  >  2:2607/1‑146 (MQ=255)                                                                        ggATGGCGATGCAGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGa                                                                      <  2:323047/147‑1 (MQ=255)                                                                          aTGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGa                                                                   <  4:1008/148‑1 (MQ=255)                                                                          aTGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCAT‑AAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGAACGAACAGGGGATTCGTGAAGTCAGCAACCCTTCGGCAATATTCTTAAGTCGCGGCGATGaa                                                                  <  1:160595/148‑1 (MQ=255)                                                                            ggCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAAGCCTTCGGCAATTTTCTTAAGTCGCGGCGATGaa                                                                  <  2:212573/147‑1 (MQ=255)                                                                               gATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGa                                                              <  3:214974/148‑1 (MQ=255)                                                                               gATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGa                                                              <  6:76435/148‑1 (MQ=255)                                                                                aTGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGAc                                                             >  2:222651/1‑148 (MQ=255)                                                                                         cccGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAg                                                    >  1:22497/1‑148 (MQ=255)                                                                                              tttCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGGGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAg                                               >  3:324955/1‑148 (MQ=255)                                                                                              tttCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGGGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAg                                               >  3:355109/1‑148 (MQ=255)                                                                                              tttCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGa                                                                   >  1:9314/1‑128 (MQ=255)                                                                                              tttCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGa                                                                   >  3:317378/1‑128 (MQ=255)                                                                                              tttCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGa                                                                   <  4:317378/128‑1 (MQ=255)                                                                                              tttCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGa                                                                   <  2:9314/128‑1 (MQ=255)                                                                                              tttCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAg                                               >  5:81618/1‑148 (MQ=255)                                                                                               ttCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGGGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAg                                               <  4:355109/147‑1 (MQ=255)                                                                                               ttCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGGGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAg                                               <  4:324955/147‑1 (MQ=255)                                                                                                 cGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCgcggc                                                                        <  2:295625/120‑1 (MQ=255)                                                                                                 cGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCgcggc                                                                        >  1:295625/1‑120 (MQ=255)                                                                                                 cGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTg                                             <  7:324895/147‑1 (MQ=255)                                                                                                 cGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTg                                             <  6:81618/147‑1 (MQ=255)                                                                                                 cGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTg                                             <  1:222651/147‑1 (MQ=255)                                                                                                 cGCACCTTGCGCAGCCATAAAAAACGCTTCTGCGCGGGGAATGAGCTGGGCGTCTTCGCGATGTCCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTGCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTg                                             <  6:359774/147‑1 (MQ=255)                                                                                                   cACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGAtg                                          >  8:240034/1‑148 (MQ=255)                                                                                                       ttGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGAcc                                                            <  7:131033/126‑1 (MQ=255)                                                                                                       ttGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGAcc                                                            >  8:131033/1‑126 (MQ=255)                                                                                                         gcgcAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATggtggt                                     <  1:322518/147‑1 (MQ=255)                                                                                                                 aTAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGggaag                             >  8:395320/1‑147 (MQ=255)                                                                                                                  tAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATggtggt                                     <  2:221870/138‑1 (MQ=255)                                                                                                                  tAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATggtggt                                     >  1:221870/1‑138 (MQ=255)                                                                                                                  tAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGggaagga                           <  5:304039/148‑1 (MQ=255)                                                                                                                  tAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTAGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGggaagga                           <  2:318115/148‑1 (MQ=255)                                                                                                                   aaaaaCCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGa                                                              >  4:78505/1‑112 (MQ=255)                                                                                                                   aaaaaCCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGa                                                              <  3:78505/112‑1 (MQ=255)                                                                                                                   aaaaaCCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGggaaggaa                          >  8:398752/1‑148 (MQ=255)                                                                                                                      aaCCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAAcgc                       <  7:398752/148‑1 (MQ=255)                                                                                                                          gCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTcc                   <  7:395320/148‑1 (MQ=255)                                                                                                                                gcgcGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCActgct             <  2:22497/148‑1 (MQ=255)                                                                                                                                           gAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTc  >  8:261856/1‑148 (MQ=255)                                                                                                                                            |                                                                                                                                                   CACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTC  >  NC_000913/4626569‑4626854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑