Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,014,199 C→T 5.8% T314I (ACC→ATC)  pqiB → paraquat‑inducible, SoxRS‑regulated MCE domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,014,1990CT 5.8% 230.1 / 2.6 69T314I (ACC→ATC) pqiBparaquat‑inducible, SoxRS‑regulated MCE domain protein
Reads supporting (aligned to +/- strand):  ref base C (30/35);  new base T (2/2);  total (32/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

TCGTTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGATGTTGTTGAGCACC  >  NC_000913/1014061‑1014336
                                                                                                                                          |                                                                                                                                         
tCGTTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCa                                                                                                                                   >  1:51681/1‑147 (MQ=255)
tCGTTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGCGTACCGTAAGCa                                                                                                                                   >  6:373228/1‑147 (MQ=255)
  gTTTTTAATGATGATCAGAAGAGCATTCATGATTCGTTGTACACCTAGCACATTGATTATCTGATGATCTTTAAAGATTCGGTAAGCTGGCTGCAACCGTGAGCTCCGGTAGAGTTCCGTGTTATTCGCCTGGGTACCGTAAGCaaa                                                                                                                                 <  5:256829/147‑1 (MQ=255)
  gTTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCaaa                                                                                                                                 <  3:28744/147‑1 (MQ=255)
  gTTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCaaa                                                                                                                                 <  7:220822/147‑1 (MQ=255)
    tttGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACGGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTg                                                                                                                              >  6:11737/1‑148 (MQ=255)
    tttGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTg                                                                                                                              >  3:185947/1‑148 (MQ=255)
     ttGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTg                                                                                                                              <  1:241173/147‑1 (MQ=255)
     ttGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACAGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGc                                                                                                                             >  7:168180/1‑148 (MQ=255)
           gatgatCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCaa                                                                                                                                  <  8:33299/137‑1 (MQ=255)
           gatgatCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCaa                                                                                                                                  >  7:33299/1‑137 (MQ=255)
            atgatCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCtt                                                                                                                      >  3:248430/1‑148 (MQ=255)
             tgatCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCttt                                                                                                                     <  5:373228/148‑1 (MQ=255)
             tgatCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCttt                                                                                                                     <  4:185947/148‑1 (MQ=255)
                     agagCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGaa                                                                                                             <  4:248430/148‑1 (MQ=255)
                     agagCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGaa                                                                                                             <  2:51681/148‑1 (MQ=255)
                      gagCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAat                                                                                                            <  4:69804/148‑1 (MQ=255)
                      gagCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAat                                                                                                            <  8:259921/148‑1 (MQ=255)
                      gagCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAat                                                                                                            <  1:185970/148‑1 (MQ=255)
                      gagCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAat                                                                                                            <  1:169934/148‑1 (MQ=255)
                      gagCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCAGAat                                                                                                            <  5:34108/148‑1 (MQ=255)
                            tCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGt                                                                                                      >  7:13542/1‑148 (MQ=255)
                             cAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCaa                                                                                                                                  <  5:75453/119‑1 (MQ=255)
                             cAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCaa                                                                                                                                  >  6:75453/1‑119 (MQ=255)
                             cAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGt                                                                                                      >  2:389281/1‑147 (MQ=255)
                             cAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGt                                                                                                      >  7:48700/1‑147 (MQ=255)
                              aGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTc                                                                                                     <  8:13542/147‑1 (MQ=255)
                               ggATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTATCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGt                                                                                                      >  8:248852/1‑145 (MQ=255)
                               ggATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTATCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGt                                                                                                      >  8:242681/1‑145 (MQ=255)
                               ggATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGcc                                                                                                                <  5:5321/135‑1 (MQ=255)
                               ggATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGcc                                                                                                                >  6:5321/1‑135 (MQ=255)
                                 aTTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGa                                                                                                  >  2:215065/1‑147 (MQ=255)
                                 aTTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGAc                                                                                                 <  4:223651/148‑1 (MQ=255)
                                       tGTACACCGATCACATTGATTATCTGACGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTaa                                                                                           >  3:297025/1‑148 (MQ=255)
                                             ccGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACgatga                                                                                     <  1:215065/148‑1 (MQ=255)
                                                    caTTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACgatg                                                                                      >  5:194857/1‑140 (MQ=255)
                                                    caTTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACgatg                                                                                      <  6:194857/140‑1 (MQ=255)
                                                       tGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTAtt                                                                           >  6:51977/1‑148 (MQ=255)
                                                            aTCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCgg                                                                       <  1:389281/147‑1 (MQ=255)
                                                             tCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTATCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGt                                                                      <  7:248852/147‑1 (MQ=255)
                                                             tCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTATCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGt                                                                      <  7:242681/147‑1 (MQ=255)
                                                             tCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGt                                                                      <  4:297025/147‑1 (MQ=255)
                                                                         tAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATc                                                         >  1:386571/1‑148 (MQ=255)
                                                                          aaaGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATc                                                         >  6:2893/1‑147 (MQ=255)
                                                                           aaGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCg                                                        <  5:11737/147‑1 (MQ=255)
                                                                                        cgGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCt                                           >  7:138615/1‑147 (MQ=255)
                                                                                         ggTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTg                                          >  7:203612/1‑147 (MQ=255)
                                                                                                  ccGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGc                                <  8:203612/148‑1 (MQ=255)
                                                                                                           ccGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAGGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGaaa                                       <  2:359209/132‑1 (MQ=255)
                                                                                                           ccGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAGGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGaaa                                       >  1:359209/1‑132 (MQ=255)
                                                                                                             ggTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAAt                     <  5:51977/148‑1 (MQ=255)
                                                                                                                 gagTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCgg                 <  8:138615/148‑1 (MQ=255)
                                                                                                                  agTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCgg                 <  8:48700/147‑1 (MQ=255)
                                                                                                                     tCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTAt                                                          >  4:27982/1‑103 (MQ=255)
                                                                                                                     tCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTAt                                                          <  3:27982/103‑1 (MQ=255)
                                                                                                                     tCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATATAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGAtgt             >  4:256780/1‑148 (MQ=255)
                                                                                                                        ggggTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGAtgttgt          <  5:2893/148‑1 (MQ=255)
                                                                                                                             aTTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGAtgtt            <  6:22311/141‑1 (MQ=255)
                                                                                                                             aTTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGAtgtt            >  5:22311/1‑141 (MQ=255)
                                                                                                                               tCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGATGTTGTTGAGCAc   >  5:384019/1‑148 (MQ=255)
                                                                                                                                cGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCa                                                   >  1:344915/1‑99 (MQ=255)
                                                                                                                                cGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCa                                                   <  2:344915/99‑1 (MQ=255)
                                                                                                                                cGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCgg                                             <  1:26604/105‑1 (MQ=255)
                                                                                                                                cGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCgg                                             >  2:26604/1‑105 (MQ=255)
                                                                                                                                cGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGAtgttg           <  6:126420/139‑1 (MQ=255)
                                                                                                                                cGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGAtgttg           >  5:126420/1‑139 (MQ=255)
                                                                                                                                cGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGATGTTGTTGAGCAcc  >  2:114826/1‑148 (MQ=255)
                                                                                                                                     gggTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGcc                                                    >  6:188018/1‑93 (MQ=255)
                                                                                                                                     gggTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGcc                                                    <  5:188018/93‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
TCGTTTTGTATGATGATCAGAAGAGCATTCAGGATTCGTTGTACACCGATCACATTGATTATCTGATGTTCTTTAAAGATTCGGTACGCGGTCTGCAACCGGGAGCTCCGGTAGAGTTCCGGGGTATTCGCCTGGGTACCGTAAGCAAAGTGCCATTCTTTGCGCCGAATATGCGTCAGACATTTAACGATGATTACCGTATTCCGGTACTGATTCGTATCGAGCCAGAGCGGCTGAAAATGCAGCTTGGCGAAAATGCGGATGTTGTTGAGCACC  >  NC_000913/1014061‑1014336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: