Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,158,881 C→T 100% S338L (TCG→TTG)  ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,158,8810CT100.0% 247.7 / NA 69S338L (TCG→TTG) ptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/33);  total (36/33)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGTTCCTGACCGGTATCACCGAGCCGATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCG  >  NC_000913/1158740‑1159025
                                                                                                                                             |                                                                                                                                                
cGTTCCTGACCGGTATCACCGAGCCGATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACgg                                                                                                                                            >  2:263232/1‑148 (MQ=255)
   tCCTGACCGGTATCACCGAGCCGATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGc                                                                                                                                                >  5:324690/1‑141 (MQ=255)
   tCCTGACCGGTATCACCGAGCCGATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGc                                                                                                                                                <  6:324690/141‑1 (MQ=255)
        aCCGGTATCACCGAGCCGATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATc                                                                                                                                     <  1:263232/147‑1 (MQ=255)
               tCACCGAGCCGATCGAGTTCTCCTTCATGGTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGTCCTGGCATTCCCAATCTGTATTCTTCTGTGGATGCGTGACGGTACGGCGTTCTTGCACGGTCTGATCGACTTCAt                                                                                                                             <  4:270262/148‑1 (MQ=255)
                 aCCGAGCCGATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCg                                                                                                                           >  5:284978/1‑148 (MQ=255)
                  ccGAGCCGATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACGTCATCGt                                                                                                                          >  8:272099/1‑148 (MQ=255)
                       ccGATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTtctgt                                                                                                                     <  6:126742/148‑1 (MQ=255)
                         gATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTtctgtct                                                                                                                   <  8:121829/148‑1 (MQ=255)
                           tCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTgg                                                                                                                 >  2:169618/1‑148 (MQ=255)
                                 tctcCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTaa                                                                                                              >  7:214167/1‑145 (MQ=255)
                                  ctcCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAAc                                                                                                             <  8:214167/145‑1 (MQ=255)
                                           gTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTtc                                                                                                                        >  1:248298/1‑125 (MQ=255)
                                           gTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTtc                                                                                                                        <  2:248298/125‑1 (MQ=255)
                                            ttCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACtgt                                                                                                >  4:352100/1‑148 (MQ=255)
                                                 tGCGCCGATCCTGTACATCATCCACGCGATTCAGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTg                                                                                           >  2:225079/1‑148 (MQ=255)
                                                       gATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCg                                                                                     <  3:352100/148‑1 (MQ=255)
                                                         tCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGAt                                                                                   >  5:79137/1‑148 (MQ=255)
                                                         tCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGAt                                                                                   >  8:42920/1‑148 (MQ=255)
                                                              tACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCTATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGAtcgtc                                                                               <  4:240310/147‑1 (MQ=255)
                                                              tACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGAtcgtcg                                                                              <  1:169618/148‑1 (MQ=255)
                                                                  tcatcCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTAt                                                                          <  6:284978/148‑1 (MQ=255)
                                                                    atcCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGGACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATc                                                                         <  4:114105/147‑1 (MQ=255)
                                                                      ccACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGt                                                                      >  8:27371/1‑148 (MQ=255)
                                                                      ccACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGt                                                                      >  7:118716/1‑148 (MQ=255)
                                                                       cACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGtt                                                                     <  6:79137/148‑1 (MQ=255)
                                                                                    cAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTta                                                        <  3:330167/148‑1 (MQ=255)
                                                                                           ggCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTc                                                                                        >  1:174338/1‑109 (MQ=255)
                                                                                           ggCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTc                                                                                        <  2:174338/109‑1 (MQ=255)
                                                                                           ggCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGttt                                                         <  1:195645/140‑1 (MQ=255)
                                                                                           ggCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGttt                                                         >  2:195645/1‑140 (MQ=255)
                                                                                           ggCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTacac                                                  >  3:241807/1‑147 (MQ=255)
                                                                                           ggCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTacac                                                  >  1:114390/1‑147 (MQ=255)
                                                                                           ggCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTacac                                                  >  8:146418/1‑147 (MQ=255)
                                                                                           ggCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTacac                                                  >  7:251876/1‑147 (MQ=255)
                                                                                                tCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTaa                                                                                                              >  8:377181/1‑82 (MQ=255)
                                                                                                tCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTaa                                                                                                              <  7:377181/82‑1 (MQ=255)
                                                                                                tCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGtt                                                                     <  1:198506/123‑1 (MQ=255)
                                                                                                tCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGtt                                                                     >  2:198506/1‑123 (MQ=255)
                                                                                                tCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCt                                             >  8:271039/1‑147 (MQ=255)
                                                                                                 cccAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCtt                                            <  7:271039/147‑1 (MQ=255)
                                                                                                 cccAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCtt                                            <  4:241807/147‑1 (MQ=255)
                                                                                                  ccAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGc                                                                                             <  1:344464/97‑1 (MQ=255)
                                                                                                  ccAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTcc                                          >  3:190689/1‑148 (MQ=255)
                                                                                                       cTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTg                                     >  2:378983/1‑148 (MQ=255)
                                                                                                          tATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTg                                  <  2:114390/148‑1 (MQ=255)
                                                                                                             tcttctGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGtt                                                                     >  7:325127/1‑110 (MQ=255)
                                                                                                             tcttctGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGtt                                                                     <  8:325127/110‑1 (MQ=255)
                                                                                                                tctGGGGATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTa                              >  6:268841/1‑146 (MQ=255)
                                                                                                                    gggATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAg                           <  8:118716/145‑1 (MQ=255)
                                                                                                                    gggATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGc                          <  7:42920/146‑1 (MQ=255)
                                                                                                                    gggATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGc                          <  7:27371/146‑1 (MQ=255)
                                                                                                                    gggATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTT‑CCGATCGGCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGc                          <  1:225079/145‑1 (MQ=255)
                                                                                                                     ggATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGttt                                                         <  6:41436/114‑1 (MQ=255)
                                                                                                                     ggATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGttt                                                         >  5:41436/1‑114 (MQ=255)
                                                                                                                     ggATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTg                                     >  7:393336/1‑134 (MQ=255)
                                                                                                                     ggATGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTg                                     <  8:393336/134‑1 (MQ=255)
                                                                                                                        tGCGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGa                    >  5:385042/1‑148 (MQ=255)
                                                                                                                          cGTGACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATc                  <  4:190689/148‑1 (MQ=255)
                                                                                                                             gACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTACAGCACTGGATCTg                >  4:230264/1‑147 (MQ=255)
                                                                                                                             gACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGa                    >  4:62126/1‑143 (MQ=255)
                                                                                                                             gACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGa                    <  3:62126/143‑1 (MQ=255)
                                                                                                                             gACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATc                  <  6:30155/145‑1 (MQ=255)
                                                                                                                             gACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATc                  >  5:30155/1‑145 (MQ=255)
                                                                                                                             gACGGTACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTg                >  7:71832/1‑147 (MQ=255)
                                                                                                                                  tACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGaaa             <  6:327255/145‑1 (MQ=255)
                                                                                                                                  tACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGaaa             >  5:327255/1‑145 (MQ=255)
                                                                                                                                  tACGTCGTTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACg          >  2:227530/1‑148 (MQ=255)
                                                                                                                                       cgtTCTTGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGAACTGGATCTGAAAACGCCggg     >  8:13063/1‑148 (MQ=255)
                                                                                                                                          tcttGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCg  >  6:128874/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                
CGTTCCTGACCGGTATCACCGAGCCGATCGAGTTCTCCTTCATGTTCGTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCG  >  NC_000913/1158740‑1159025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑