| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,161,067 | C→T | 6.3% | R162H (CGT→CAT) | fhuE ← | ferric‑rhodotorulic acid outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,161,067 | 0 | C | T | 6.3% | 204.1 / 2.8 | 63 | R162H (CGT→CAT) | fhuE | ferric‑rhodotorulic acid outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base C (28/31); new base T (2/2); total (30/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01 | |||||||||||
TGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGATCTGGAATCCGCGGGAATAATAAAGAGCACGATC > NC_000913/1160920‑1161206 | tGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACTACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCAc > 5:36400/1‑148 (MQ=255)tGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCAc > 3:24368/1‑148 (MQ=255) tAATTCCGCCACATACCGTTCTTTGTTCCAGATACCTTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATTTATTACCCTTCCCGGTAATGAGTACTGTCGCGCCACGc < 6:233766/148‑1 (MQ=255) tAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGc < 4:24368/148‑1 (MQ=255) tAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGc < 8:226144/148‑1 (MQ=255) aTCCGCCACATACCGTGCTTTGTTCCAGCTACCGTATTCCGCCGCGACATCGCCTTTAAATTCACGACTGGTCGCGGGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCact < 5:76357/148‑1 (MQ=255) tCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCacta < 5:310388/148‑1 (MQ=255) cacaTACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCt < 6:36400/148‑1 (MQ=255) cacaTACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCt < 8:132976/148‑1 (MQ=255) acaTACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTa < 5:317055/148‑1 (MQ=255) tACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTAcgc > 7:246063/1‑148 (MQ=255) tACCGTTCTTTGTTCCAGCTACCATATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTAcgc < 7:326116/148‑1 (MQ=255) aCCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTAcgcg > 1:330597/1‑148 (MQ=255) cTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCaa < 8:246063/147‑1 (MQ=255) cTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCaa > 8:141789/1‑147 (MQ=255) tttGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAag < 2:193725/148‑1 (MQ=255) tGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAag < 2:330597/146‑1 (MQ=255) tGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAag < 2:344782/146‑1 (MQ=255) cAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGcc < 1:116536/147‑1 (MQ=255) aGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCa > 3:162140/1‑147 (MQ=255) aGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCa > 3:156825/1‑147 (MQ=255) gTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGa > 3:141031/1‑147 (MQ=255) aTTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCAc > 1:227215/1‑108 (MQ=255) aTTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCAc < 2:227215/108‑1 (MQ=255) aTTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGc < 8:253920/110‑1 (MQ=255) aTTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGc > 7:253920/1‑110 (MQ=255) cGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCATGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGAtt > 2:241386/1‑148 (MQ=255) cGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCATGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGAtt > 2:135437/1‑148 (MQ=255) gCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTc > 1:302413/1‑148 (MQ=255) tAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGc < 2:362462/89‑1 (MQ=255) tAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGc > 1:362462/1‑89 (MQ=255) aTTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCaa > 7:377749/1‑105 (MQ=255) aTTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCaa < 8:377749/105‑1 (MQ=255) tCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCactact > 2:85464/1‑90 (MQ=255) tCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCactact < 1:85464/90‑1 (MQ=255) aCGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACGCGTCCCGGTCATGAGTCCTGTCGCGCCTCGAAATACTTCTACGCGTTCAAAGAGTGCCTTATCATAAAGTGCGTCGCCCTGATTCCAGCGCGATTCa < 2:375463/148‑1 (MQ=255) cTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAAt < 2:302413/148‑1 (MQ=255) gTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCTCCCAGATTCCAGCTCGATTCAAAATAg < 4:156825/147‑1 (MQ=255) gTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAg < 4:162140/147‑1 (MQ=255) gTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAg < 4:141031/147‑1 (MQ=255) atTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAAt < 5:343129/125‑1 (MQ=255) atTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAAt > 6:343129/1‑125 (MQ=255) ttAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCata > 2:390/1‑147 (MQ=255) aTTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAg < 7:141789/147‑1 (MQ=255) aGATGGATTACCAGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATATGTGGGGATACCATCAACCATAAAGTTATCGCTc > 6:197/1‑148 (MQ=255) aTGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCat > 1:101210/1‑133 (MQ=255) aTGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCat < 2:101210/133‑1 (MQ=255) ggATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATGCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGATCTgg > 2:129737/1‑147 (MQ=255) tACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGATCTGGAATcc > 3:36574/1‑148 (MQ=255) tCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCaa < 8:33259/90‑1 (MQ=255) tCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCaa > 7:33259/1‑90 (MQ=255) tCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGATCTGGa < 1:393468/132‑1 (MQ=255) tCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGATCTGGa > 2:393468/1‑132 (MQ=255) cATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGATCTGGAATCCGCGGGAataata > 3:304043/1‑147 (MQ=255) aTGAGTCCTGTCGCGCCATGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGATCTGGAATCCGCGGGAAtaataa < 1:135437/147‑1 (MQ=255) aTGAGTCCTGTCGCGCCATGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGATCTGGAATCCGCGGGAAtaataa < 1:241386/147‑1 (MQ=255) tGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGATCTGGAATCCGCGGGAataata > 5:13906/1‑145 (MQ=255) gAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGATCTGGAATCCGCGGGAATAATaaa > 4:89630/1‑146 (MQ=255) aGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGATCTGGaa < 8:7512/128‑1 (MQ=255) aGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGATCTGGaa < 8:13356/128‑1 (MQ=255) aGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGATCTGGaa > 7:7512/1‑128 (MQ=255) aGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGATCTGGaa > 7:13356/1‑128 (MQ=255) tGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGATCTGGAATCCGCGGGAATAATAAAGAGCACGa > 6:364176/1‑148 (MQ=255) gTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGa < 7:264069/115‑1 (MQ=255) gTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGa > 8:264069/1‑115 (MQ=255) gTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCATCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCACCAACCATATAGTTATCGATCTGGAATCCGCGGGAATAATAAAGAGCACGAt > 2:62650/1‑148 (MQ=255) tCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGATCTGGAATCCGCGGGAATAATAAAGAGCACGATc > 4:88866/1‑148 (MQ=255) | TGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCAGATGGATTACCCGTCCCGGTCATGAGTCCTGTCGCGCCACGCACTACTTCTACGCGTTCAAAGAGTGCCATATCAGAAAGTGCGTCGCCCAGATTCCAGCGCGATTCAAAATAGGTGGGGATACCATCAACCATATAGTTATCGATCTGGAATCCGCGGGAATAATAAAGAGCACGATC > NC_000913/1160920‑1161206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |