Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,211,424 T→C 9.5% pseudogene (12/165 nt) icdC → pseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,211,4240TC 9.5% 182.7 / 8.2 63pseudogene (12/165 nt)icdCpseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion
Reads supporting (aligned to +/- strand):  ref base T (26/31);  new base C (3/3);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAAAATGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCG  >  NC_000913/1211277‑1211566
                                                                                                                                                   |                                                                                                                                              
tAAATATGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTACGGGCTAATGAGTAATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCat                                                                                                                                                <  5:118091/148‑1 (MQ=255)
tAAAAATGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTTATGAGTTATATGCAAATTATTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCat                                                                                                                                                <  1:209904/148‑1 (MQ=255)
 aaaaaTGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCata                                                                                                                                               >  8:64244/1‑148 (MQ=255)
 aaaaaTGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCata                                                                                                                                               >  2:242660/1‑148 (MQ=255)
 aaaaaTGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCat                                                                                                                                                <  8:320132/147‑1 (MQ=255)
 aaaaaTGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCat                                                                                                                                                >  7:320132/1‑147 (MQ=255)
  aaaaTGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCat                                                                                                                                                <  3:183343/146‑1 (MQ=255)
  aaaaTGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCat                                                                                                                                                <  6:246339/146‑1 (MQ=255)
  aaaaTGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCat                                                                                                                                                <  6:379028/146‑1 (MQ=255)
   aaaTGGTTATCAAAACTCAAAAACACATTTTCATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATg                                                                                                                                             <  5:308486/148‑1 (MQ=255)
    aaTGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATgg                                                                                                                                            <  4:19375/148‑1 (MQ=255)
     aTGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATggg                                                                                                                                           <  8:130149/148‑1 (MQ=255)
     aTGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATggg                                                                                                                                           <  7:64244/148‑1 (MQ=255)
             cAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTACCTAATGCTGCGCCACATGGGTTggccc                                                                                                                                    >  6:129232/1‑144 (MQ=255)
             cAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACt                                                                                                                                    >  1:69030/1‑147 (MQ=255)
              aaaaCTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTg                                                                                                                                   <  1:159604/147‑1 (MQ=255)
               aaaCTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGaa                                                                                                                                 <  7:65918/148‑1 (MQ=255)
                       aaaCACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGa                                                                                                                         <  2:69030/148‑1 (MQ=255)
                       aaaCACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGa                                                                                                                         <  8:163090/148‑1 (MQ=255)
                        aaCACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAc                                                                                                                        >  3:65080/1‑148 (MQ=255)
                                       tCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGt                                                                                                         <  1:242660/148‑1 (MQ=255)
                                       tCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGt                                                                                                         <  4:156516/148‑1 (MQ=255)
                                       tCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGt                                                                                                         <  5:129232/148‑1 (MQ=255)
                                        cAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCTCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTa                                                                                                        >  5:37325/1‑148 (MQ=255)
                                                tataGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGc                                                                                                                            <  7:122569/120‑1 (MQ=255)
                                                tataGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGc                                                                                                                            >  8:122569/1‑120 (MQ=255)
                                                       aTGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaatca                                                                                         <  4:65080/148‑1 (MQ=255)
                                                          cTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATg                                                                                      <  5:89023/148‑1 (MQ=255)
                                                               ggCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGaa                                                                                  >  1:368080/1‑147 (MQ=21)
                                                                   aaTGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaatca                                                                                         >  2:189209/1‑136 (MQ=255)
                                                                   aaTGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaatca                                                                                         <  1:189209/136‑1 (MQ=255)
                                                                   aaTGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaat                                                                                           >  6:381117/1‑134 (MQ=255)
                                                                   aaTGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaat                                                                                           <  5:381117/134‑1 (MQ=255)
                                                                    aTGAGTTATAAGCAAATTAGTAAAATTATGTTGCTATGACAGATAGTTACGATTTAGTCATCTAACTAATGCTGAGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCg                                                                             >  5:138163/1‑147 (MQ=255)
                                                                     tGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaatca                                                                                         >  4:238504/1‑134 (MQ=255)
                                                                     tGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaatca                                                                                         <  3:238504/134‑1 (MQ=255)
                                                                      gAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAgg                                                                                                          <  1:308915/116‑1 (MQ=255)
                                                                      gAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAgg                                                                                                          >  2:308915/1‑116 (MQ=255)
                                                                      gAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAgg                                                                                                 <  2:263646/125‑1 (MQ=255)
                                                                      gAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAgg                                                                                                 >  1:263646/1‑125 (MQ=255)
                                                                      gAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaatc                                                                                          >  3:100211/1‑132 (MQ=255)
                                                                      gAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaatc                                                                                          <  4:100211/132‑1 (MQ=255)
                                                                      gAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTaa                                                                          >  5:11018/1‑148 (MQ=255)
                                                                      gAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTa                                                                           >  8:16145/1‑147 (MQ=255)
                                                                      gAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTa                                                                           <  7:16145/147‑1 (MQ=255)
                                                                      gAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTgg                                                                                                                                       >  1:69360/1‑87 (MQ=255)
                                                                      gAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTgg                                                                                                                                       <  2:69360/87‑1 (MQ=255)
                                                                          tttatGCAAATTAGTAAAATTAAGTTGCTATGTCAGATAGTTACGAATTAGTCATCTAACTACTGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGGAATCAATGCCAAGACCGTAACtt                                                                       <  6:138163/145‑1 (MQ=255)
                                                                          tataTGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGt                                                                                                         >  3:145327/1‑113 (MQ=255)
                                                                          tataTGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGt                                                                                                         <  6:156226/113‑1 (MQ=255)
                                                                          tataTGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGt                                                                                                         <  4:145327/113‑1 (MQ=255)
                                                                          tataTGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGt                                                                                                         >  5:156226/1‑113 (MQ=255)
                                                                           atatGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTa                                                                      <  2:368080/147‑1 (MQ=18)
                                                                             atGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGa                                                                   >  3:91486/1‑148 (MQ=255)
                                                                               gCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaatca                                                                                         <  7:319957/124‑1 (MQ=255)
                                                                               gCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaatca                                                                                         >  2:314924/1‑124 (MQ=255)
                                                                               gCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaatca                                                                                         >  8:319957/1‑124 (MQ=255)
                                                                               gCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaatca                                                                                         <  1:314924/124‑1 (MQ=255)
                                                                                cAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACt                                                                 <  7:44415/147‑1 (MQ=255)
                                                                                 aaaTTAGTTAAATTATGTTGCTATGTCAGATAGTTACTATTTAGGCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTc                                                               <  6:37325/148‑1 (MQ=255)
                                                                                   aTTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaatca                                                                                         <  7:123947/120‑1 (MQ=255)
                                                                                   aTTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaatca                                                                                         >  8:123947/1‑120 (MQ=255)
                                                                                         aaaaTTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACt                                                                 >  7:264864/1‑138 (MQ=255)
                                                                                         aaaaTTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACt                                                                 <  8:264864/138‑1 (MQ=255)
                                                                                          aaaTTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTg                                                      <  4:91486/148‑1 (MQ=255)
                                                                                              tATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGTTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATg                                                   >  7:352309/1‑147 (MQ=255)
                                                                                                tGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGa                                                 <  1:312588/147‑1 (MQ=255)
                                                                                                             gATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGCCCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAgctgc                                   >  2:245403/1‑148 (MQ=255)
                                                                                                                                 ctaactaaTGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGt               >  5:207718/1‑148 (MQ=255)
                                                                                                                                             gcgcCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGatcatc   >  3:259044/1‑148 (MQ=255)
                                                                                                                                             gcgcCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGatcatc   >  2:115633/1‑148 (MQ=255)
                                                                                                                                              cgcCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTGGTGAAGCGATCATCg  >  7:335311/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
TAAAAATGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCG  >  NC_000913/1211277‑1211566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: