Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,303,990 G→A 9.9% V53I (GTA→ATA)  oppC → oligopeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,303,9900GA 9.9% 117.8 / 2.4 40V53I (GTA→ATA) oppColigopeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (17/19);  new base A (2/2);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGACTGGGCGATGATGTCCAGCGCCCCGGATATGGAGTCCGGTCACTACTTTGGTACTGACTCATCCGGTCGCGACCTGCTTGTGCGCGTTGCGATTGGC  >  NC_000913/1303847‑1304133
                                                                                                                                               |                                                                                                                                               
agAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAAt                                                                                                                                             <  2:54931/148‑1 (MQ=255)
agAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAAt                                                                                                                                             <  3:205267/148‑1 (MQ=255)
       cAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAAt                                                                                                                                             <  1:124783/141‑1 (MQ=255)
       cAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAAt                                                                                                                                             >  2:124783/1‑141 (MQ=255)
          cGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCg                                                                                                                                   <  5:329543/148‑1 (MQ=255)
                       aaTTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGt                                                                                                                      <  5:357601/148‑1 (MQ=255)
                       aaTTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGt                                                                                                                      <  3:183061/148‑1 (MQ=255)
                        aTTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCTATGCTTTCCCAGtt                                                                                                                     >  4:34910/1‑148 (MQ=255)
                             aGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCt                                                                                                                <  2:190758/148‑1 (MQ=255)
                                  aaaGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTATAATCCTGGCACCGATGCTTTCCCAg                                                                                                                       <  5:41386/136‑1 (MQ=255)
                                  aaaGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTATAATCCTGGCACCGATGCTTTCCCAg                                                                                                                       >  6:41386/1‑136 (MQ=255)
                                    aGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGa                                                                                                         <  5:283426/148‑1 (MQ=255)
                                         gatggcGAAGGGCGCAGATTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGGTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATactg                                                                                                    <  5:46940/143‑1 (MQ=255)
                                           ggTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATactgac                                                                                                  >  7:357544/1‑148 (MQ=255)
                                             tCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTATAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATactgactg                                                                                                >  4:288891/1‑148 (MQ=255)
                                              cGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTg                                                                                                                   <  2:96400/128‑1 (MQ=255)
                                              cGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTg                                                                                                                   >  1:96400/1‑128 (MQ=255)
                                                aaGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGt                                                                                                                      >  5:317173/1‑123 (MQ=255)
                                                aaGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGt                                                                                                                      <  6:317173/123‑1 (MQ=255)
                                                aaGGGCGAAGCTTGTGGCAGGACGCACGTCGACCTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGACTGGGc                                                                                             >  4:149624/1‑148 (MQ=255)
                                                    gcgcAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGACTGGGCgat                                                                                          >  6:136166/1‑147 (MQ=255)
                                                    gcgcAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTATAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGACTGGGCgatg                                                                                         <  3:288891/148‑1 (MQ=255)
                                                                     aCGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATactg                                                                                                    <  2:17229/120‑1 (MQ=255)
                                                                     aCGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATactg                                                                                                    >  1:17229/1‑120 (MQ=255)
                                                                             cGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGAt                                                                                                        >  6:353393/1‑108 (MQ=255)
                                                                             cGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGAt                                                                                                        <  5:353393/108‑1 (MQ=255)
                                                                                   tttATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGACTGGGCGATGATATCCAGCGCCCCGGATATGGAGTCCGGt                                                           >  7:303856/1‑147 (MQ=255)
                                                                                         cATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGACTGGGCGATGATGTCCAGCGCCCCGGATATGGAGTCCGGTCactac                                                     <  3:149624/147‑1 (MQ=255)
                                                                                              ccGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGACTGGGCGATGATGTCCAGCGCCCCGGATATGGAGTCCGGTCACTACTTTGGt                                               <  8:357544/148‑1 (MQ=255)
                                                                                                     gcggTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGACTGGGCgat                                                                                          <  5:331656/98‑1 (MQ=255)
                                                                                                     gcggTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGACTGGGCgat                                                                                          >  6:331656/1‑98 (MQ=255)
                                                                                                         ttGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGACTGGGCGATGATGTCCAGCGCCCCGGATATGGAGTCCGGTCACTACTTTGGTACTGACTCATc                                    >  4:44093/1‑148 (MQ=255)
                                                                                                                 cTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGACTGGGCGATGATGTCCAGCGCCCCGGATATGGAGTCCGGTCACTACTTTGGTACTGACTCATCCGGTcgc                             >  1:80220/1‑147 (MQ=255)
                                                                                                                         gatggtgctgATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGACTGGGCGATGATGTCCAGCGCCCCGGATATGGAGTCCGGTCAATACTTTGGTACTGACTCATCCGGTCGCGACCTGCtt                    >  2:35057/3‑148 (MQ=255)
                                                                                                                            ggtgctgATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGACTGGGCGATGATGTCCAGCGCCCCGGATATGGAGTCCGGTCACTACTTTGGTACTGACTCATCCGGTCGCGACCTGCTtgtg                 >  7:88335/1‑148 (MQ=255)
                                                                                                                                ctgATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGACTGGGCGATGATGTCCAGCGCCCCGGATATGGAGTCCGGTCACTACTTTGGTACTGACTCATCCGGTCGCGACCTGCTTGTGcgcg             <  3:44093/148‑1 (MQ=255)
                                                                                                                                ctgATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGACTGGGCGATGATGTCCAGCGCCCCGGATATGGAGTCCGGTCACTACTTTGGTACTGACTCATCCGGTCGCGACCTGCTTGTGcgcg             <  2:80220/148‑1 (MQ=255)
                                                                                                                                    tCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGCCTGGGCGATGATGTCCAGCGCCCCGGATATGGAGTCCGGTCACTACTTTGGTACTGACTCATCCGGTCGCGACCTGCTTGTGCGCGTTGc         >  6:178452/1‑148 (MQ=255)
                                                                                                                                          tATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGACTGGGCGATGATGTCCAGCGCCCCGGATATGGAGTCCGGTCACTACTTTGGTACTGACTCATCCGGTCGCGACCTGCTTGTGCGCGTTGCGATTgg   >  7:382553/1‑148 (MQ=255)
                                                                                                                                            tttGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGACTGGGCGATGATGTCCAGCGCCCCGGATATGGAGTCCGGTCACTACTTTGGTACTGACTCATCCGGTCGCGACCTGCTTGTGCGCGTTGCGATTggc  <  5:178452/147‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
AGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAGTCTGATAGTGCTGGTGCTGATCGCGTTATTTGTAATCCTGGCACCGATGCTTTCCCAGTTTGCCTATGACGATACTGACTGGGCGATGATGTCCAGCGCCCCGGATATGGAGTCCGGTCACTACTTTGGTACTGACTCATCCGGTCGCGACCTGCTTGTGCGCGTTGCGATTGGC  >  NC_000913/1303847‑1304133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑