Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,830,829 T→C 8.3% E385G (GAG→GGG)  astC ← succinylornithine transaminase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,830,8290TC 8.3% 137.6 / 3.0 48E385G (GAG→GGG) astCsuccinylornithine transaminase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (22/22);  new base C (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGACGTCAGGCCGCCGCCCGTTTTGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAA  >  NC_000913/1830682‑1830975
                                                                                                                                                   |                                                                                                                                                  
cGACGTCAGGCCGCCGCCCGTTTTGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACct                                                                                                                                                    >  3:158790/1‑148 (MQ=255)
cGACGTCAGGCCGCCGCCCGTTTTGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACct                                                                                                                                                    >  7:230569/1‑148 (MQ=255)
 gACGTCAGGCCGCCGCCCGTTTTGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCCCctc                                                                                                                                                   >  5:286214/1‑148 (MQ=255)
  aCGTCAGGCCGCCGCCCGTTTTGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACctct                                                                                                                                                  <  2:226651/148‑1 (MQ=255)
                  cGTTTTGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGa                                                                                                                                  <  8:230569/148‑1 (MQ=255)
                     tttGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACctct                                                                                                                                                  <  3:135945/129‑1 (MQ=255)
                     tttGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACctct                                                                                                                                                  >  4:135945/1‑129 (MQ=255)
                     tttGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAgcg                                                                                                                               >  1:96182/1‑148 (MQ=255)
                      ttGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAgcg                                                                                                                               <  6:286214/147‑1 (MQ=255)
                       tGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAgcgc                                                                                                                              <  2:96182/147‑1 (MQ=255)
                       tGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAgcgc                                                                                                                              <  7:24037/147‑1 (MQ=255)
                        gCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAg                                                                                                                            >  5:341966/1‑148 (MQ=255)
                               aGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCaaa                                                                                                                     <  6:341966/148‑1 (MQ=255)
                               aGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCaaa                                                                                                                     <  4:158790/148‑1 (MQ=255)
                                gCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCttcttc                                                                                                                                             <  6:122767/123‑1 (MQ=255)
                                gCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCttcttc                                                                                                                                             >  5:122767/1‑123 (MQ=255)
                                  tGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACg                                                                                                                  >  1:262120/1‑148 (MQ=255)
                                       cAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGAc                                                                                                                                       <  7:151809/122‑1 (MQ=255)
                                       cAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGAc                                                                                                                                       >  8:151809/1‑122 (MQ=255)
                                                    tCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGca                                                                                                                >  6:322288/1‑132 (MQ=255)
                                                    tCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGca                                                                                                                <  5:322288/132‑1 (MQ=255)
                                                    tCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCg                                                                                                 >  1:231677/1‑147 (MQ=255)
                                                     cTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGc                                                                                                >  1:251104/1‑147 (MQ=255)
                                                        aTCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATc                                                                                            <  2:262120/148‑1 (MQ=255)
                                                           gCTCAACGGGACGGATGACCATCATGATGAACCTCTGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAg                                                                                          <  2:231677/147‑1 (MQ=255)
                                                                aCGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCCCCCCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCCCCCGCAATCAGTAcccg                                                                                    >  2:251140/1‑146 (MQ=255)
                                                                aCGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCCCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCAGCAATCAGTACCAt                                                                                    >  2:245404/1‑148 (MQ=255)
                                                                 cGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCAt                                                                                    >  4:72411/1‑147 (MQ=255)
                                                                         aTGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCaaa                                                                                                                     <  1:163899/106‑1 (MQ=255)
                                                                         aTGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCaaa                                                                                                                     >  2:163899/1‑106 (MQ=255)
                                                                         aTGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGc                                                                           <  4:176541/148‑1 (MQ=255)
                                                                            aCCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCtt                                                                         <  2:251104/147‑1 (MQ=255)
                                                                                tcatGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTcgcc                                                                    >  3:77766/1‑148 (MQ=255)
                                                                                 catGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTcgccg                                                                   >  4:157057/1‑148 (MQ=255)
                                                                                              ggCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTga                                                           >  4:87531/1‑143 (MQ=255)
                                                                                                   aCAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGc                                                                                                                 >  2:336910/1‑84 (MQ=255)
                                                                                                   aCAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGc                                                                                                                 <  1:336910/84‑1 (MQ=255)
                                                                                                   aCAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGtt                                                 <  3:157057/148‑1 (MQ=255)
                                                                                                   aCAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCCCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGtt                                                 <  1:251140/148‑1 (MQ=255)
                                                                                                   aCAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCCCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGtt                                                 <  1:245404/148‑1 (MQ=255)
                                                                                                    cAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGttt                                                >  8:225918/1‑148 (MQ=255)
                                                                                                             cGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTga                                                           <  2:352723/128‑1 (MQ=255)
                                                                                                             cGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTga                                                           >  1:352723/1‑128 (MQ=255)
                                                                                                                          aaaGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCa                          <  3:72411/148‑1 (MQ=255)
                                                                                                                            aGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCAtt                        >  6:331448/1‑148 (MQ=255)
                                                                                                                            aGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCAtt                        >  4:87979/1‑148 (MQ=255)
                                                                                                                            aGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCAtt                        >  6:348423/1‑148 (MQ=255)
                                                                                                                              cGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCa                      <  7:225918/148‑1 (MQ=255)
                                                                                                                                aTCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGt                    <  4:77766/148‑1 (MQ=255)
                                                                                                                                 tCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGt                    >  2:185617/1‑147 (MQ=255)
                                                                                                                                             gtcACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATc        <  3:87531/147‑1 (MQ=255)
                                                                                                                                               cACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAg      >  5:366531/1‑147 (MQ=255)
                                                                                                                                                aCCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGt                    >  6:178349/1‑132 (MQ=255)
                                                                                                                                                aCCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGt                    <  5:178349/132‑1 (MQ=255)
                                                                                                                                                  ctctTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAAt                               <  1:295671/119‑1 (MQ=255)
                                                                                                                                                  ctctTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAAt                               >  2:295671/1‑119 (MQ=255)
                                                                                                                                                  ctctTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCaaa  >  3:62162/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGACGTCAGGCCGCCGCCCGTTTTGCTGGCAAGCTGCATCAGCGCCGAGACATCTGATCGCTCAACGGGACGGATGACCATCATGATGAACCTCGGCTAACAAAGTGTTCGCAAGCAGCTGCAAAGCGATCCAGTCCGGTCGTCACCTCTTCTTCGCTGACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAA  >  NC_000913/1830682‑1830975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑