Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,872,748 G→A 5.8% V236V (GTG→GTA yeaJ → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,872,7480GA 5.8% 231.4 / 2.7 69V236V (GTG→GTAyeaJputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base G (39/26);  new base A (2/2);  total (41/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.69e-01

GTATTGGCATCGATCGCAAAGATATTGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTCC  >  NC_000913/1872603‑1872893
                                                                                                                                                 |                                                                                                                                                 
gTATTGGCATCGATCGCAAAGATATTGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGtgct                                                                                                                                                 <  4:75315/148‑1 (MQ=255)
     ggCATCGATCGCAAAGATATTGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGctgctg                                                                                                                                             >  6:29587/1‑147 (MQ=255)
             tcgCAAAGATATTGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTaa                                                                                                                                     >  1:367555/1‑147 (MQ=255)
                     atatTGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGTGATTGTGCTGCTGGATATTAACAAAAACaa                                                                                                                            <  2:281915/148‑1 (MQ=255)
                     atatTGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACaa                                                                                                                            <  7:348284/148‑1 (MQ=255)
                      tatTGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGTATTGTGCTGCTGGATATTAACAAAAACaa                                                                                                                            <  7:177487/147‑1 (MQ=255)
                           aaaaGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGc                                                                                                                       <  5:29587/147‑1 (MQ=255)
                            aaaGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCg                                                                                                                      >  1:38742/1‑147 (MQ=255)
                                    ttACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCtt                                                                                                             >  2:112997/1‑148 (MQ=255)
                                     tACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCtt                                                                                                             <  2:367555/147‑1 (MQ=255)
                                       ccGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCtttt                                                                                                           <  7:174242/147‑1 (MQ=255)
                                               aCCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCAt                                                                                                   >  3:151967/1‑147 (MQ=255)
                                                ccGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATg                                                                                                  <  2:38742/147‑1 (MQ=255)
                                                                ttACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATg                                                                                                  >  4:74564/1‑131 (MQ=255)
                                                                ttACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATg                                                                                                  <  3:74564/131‑1 (MQ=255)
                                                                ttACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTgg                                                                                 >  2:67018/1‑148 (MQ=255)
                                                                ttACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTgg                                                                                 >  3:319470/1‑148 (MQ=255)
                                                                ttACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTgg                                                                                 >  5:87480/1‑148 (MQ=255)
                                                                ttACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTgg                                                                                 >  8:340898/1‑148 (MQ=255)
                                                                 tACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGa                                                                                                 >  6:384919/1‑131 (MQ=255)
                                                                 tACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGa                                                                                                 <  5:384919/131‑1 (MQ=255)
                                                                 tACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGc                                                                                >  8:226785/1‑148 (MQ=255)
                                                                         tCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCtttt                                                                                                           >  7:340178/1‑113 (MQ=255)
                                                                         tCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCtttt                                                                                                           >  7:60060/1‑113 (MQ=255)
                                                                         tCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCtttt                                                                                                           <  8:340178/113‑1 (MQ=255)
                                                                         tCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCtttt                                                                                                           <  8:60060/113‑1 (MQ=255)
                                                                         tCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTtct                                                                         >  8:228626/1‑147 (MQ=255)
                                                                         tCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTtct                                                                         >  6:30204/1‑147 (MQ=255)
                                                                           aGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCa                                                                       <  7:226785/147‑1 (MQ=255)
                                                                                aCTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCATCTCCTCTGGCGTTTTCTCAATGTCa                                                                 >  5:342018/1‑148 (MQ=255)
                                                                                   aaaCAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTc                                                                                                     >  4:358923/1‑109 (MQ=255)
                                                                                   aaaCAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTc                                                                                                     <  3:358923/109‑1 (MQ=255)
                                                                                   aaaCAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGc                                                              >  1:307745/1‑148 (MQ=255)
                                                                                   aaaCAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGc                                                              >  7:317775/1‑148 (MQ=255)
                                                                                     aCAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCt                                                             >  3:280018/1‑147 (MQ=255)
                                                                                         aaCGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAacc                                                         >  6:247633/1‑147 (MQ=255)
                                                                                            gTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAaccgata                                                     <  5:30204/148‑1 (MQ=255)
                                                                                            gTGATGAGTTTGCTGACGCCGCTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAaccgata                                                     >  6:53420/1‑148 (MQ=255)
                                                                                              gatgaGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGt                                                                   <  7:184059/132‑1 (MQ=255)
                                                                                              gatgaGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGt                                                                   >  8:184059/1‑132 (MQ=255)
                                                                                              gatgaGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAaccgatacc                                                   >  4:210671/1‑147 (MQ=255)
                                                                                                 gaGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAaccgataccgat                                                >  7:311319/1‑148 (MQ=255)
                                                                                                  aGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGAtt                                               <  7:340898/148‑1 (MQ=255)
                                                                                                   gTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTc                                              <  4:319470/148‑1 (MQ=255)
                                                                                                          gACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGgcgc                                       >  2:148229/1‑148 (MQ=255)
                                                                                                                 gTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTa                                <  6:87480/148‑1 (MQ=255)
                                                                                                                  tataTGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCa                                                                       <  6:43014/108‑1 (MQ=255)
                                                                                                                  tataTGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCa                                                                       >  5:43014/1‑108 (MQ=255)
                                                                                                                  tataTGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTat                               <  4:151967/148‑1 (MQ=255)
                                                                                                                  tataTGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTat                               <  1:112997/148‑1 (MQ=255)
                                                                                                                  tataTGTCGCAGGGCAGCTAAAAGGGATTGTACTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAaccgatacc                                                   <  4:178531/128‑1 (MQ=255)
                                                                                                                  tataTGTCGCAGGGCAGCTAAAAGGGATTGTACTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAaccgatacc                                                   >  3:80952/1‑128 (MQ=255)
                                                                                                                  tataTGTCGCAGGGCAGCTAAAAGGGATTGTACTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAaccgatacc                                                   >  3:178531/1‑128 (MQ=255)
                                                                                                                  tataTGTCGCAGGGCAGCTAAAAGGGATTGTACTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAaccgatacc                                                   <  4:80952/128‑1 (MQ=255)
                                                                                                                    tatGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTatc                              <  4:280018/147‑1 (MQ=255)
                                                                                                                        tCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTAtcatca                          <  7:228626/147‑1 (MQ=255)
                                                                                                                                cAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAAcc                       <  4:70187/142‑1 (MQ=255)
                                                                                                                                cAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAAcc                       >  3:70187/1‑142 (MQ=255)
                                                                                                                                cAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCATCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGc                  >  7:354731/1‑147 (MQ=255)
                                                                                                                                   cTAAAAGGGATTGTGCTGCTGGATATTAACAAAAAAAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCCATTCGGGGCGCGACATTATCATCAACAAGAGCg                 >  2:239953/1‑145 (MQ=255)
                                                                                                                                    tAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTatcatc                           >  7:218266/1‑134 (MQ=255)
                                                                                                                                    tAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTatcatc                           <  8:218266/134‑1 (MQ=255)
                                                                                                                                    tAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCg                 >  2:361039/1‑144 (MQ=255)
                                                                                                                                      aaaGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGAt            >  2:205091/1‑147 (MQ=255)
                                                                                                                                      aaaGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGAt            >  5:344815/1‑147 (MQ=255)
                                                                                                                                       aaGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATa           >  8:147105/1‑147 (MQ=255)
                                                                                                                                         gggATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATc        >  3:285415/1‑148 (MQ=255)
                                                                                                                                         gggATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATc        >  1:56470/1‑148 (MQ=255)
                                                                                                                                             ttGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTatc                              >  7:177641/1‑122 (MQ=255)
                                                                                                                                             ttGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTatc                              <  8:177641/122‑1 (MQ=255)
                                                                                                                                              tgtgCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTc   >  5:287342/1‑148 (MQ=255)
                                                                                                                                                tgctgcTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTcc  <  5:53420/147‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GTATTGGCATCGATCGCAAAGATATTGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTCC  >  NC_000913/1872603‑1872893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: