Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,890,091 G→C 6.9% H148D (CAT→GAT)  yeaY ← Slp family lipoprotein, RpoE‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,890,0910GC 6.9% 190.8 / 2.9 55H148D (CAT→GAT) yeaYSlp family lipoprotein, RpoE‑regulated
Reads supporting (aligned to +/- strand):  ref base G (27/24);  new base C (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACAT  >  NC_000913/1889953‑1890234
                                                                                                                                          |                                                                                                                                               
attCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATCCCAACGttt                                                                                                                                        >  8:305015/1‑148 (MQ=255)
attCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATCCCAACGttt                                                                                                                                        >  8:299718/1‑148 (MQ=255)
          aaCTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAAAGCCAACGTTTGTAACCCGtt                                                                                                                              <  2:215145/148‑1 (MQ=255)
                                   ggATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAgg                                                                                                     <  5:309667/148‑1 (MQ=255)
                                    gATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTta                                                                                                         >  7:256596/1‑143 (MQ=255)
                                    gATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTta                                                                                                         <  8:256596/143‑1 (MQ=255)
                                     aTTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAAcc                                                                                                                                  <  8:28286/117‑1 (MQ=255)
                                     aTTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAAcc                                                                                                                                  >  7:28286/1‑117 (MQ=255)
                                     aTTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAAcc                                                                                                                                  <  6:353138/117‑1 (MQ=255)
                                     aTTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAAcc                                                                                                                                  >  5:353138/1‑117 (MQ=255)
                                        ataCCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATCCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTa                                                                                                <  7:299718/148‑1 (MQ=255)
                                         taCCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTa                                                                                                <  5:186090/147‑1 (MQ=255)
                                         taCCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATCCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTa                                                                                                <  7:305015/147‑1 (MQ=255)
                                            cAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAAc                                                                                                                                   <  7:136810/109‑1 (MQ=255)
                                            cAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAAc                                                                                                                                   >  8:136810/1‑109 (MQ=255)
                                                cccATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGAt                                                                                        >  3:343282/1‑148 (MQ=255)
                                                     ccgccgTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTtat                                                                                                        <  3:281198/127‑1 (MQ=255)
                                                     ccgccgTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTtat                                                                                                        >  4:281198/1‑127 (MQ=255)
                                                     ccgccgTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTg                                                                                             >  1:332377/1‑138 (MQ=255)
                                                     ccgccgTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTg                                                                                             <  2:332377/138‑1 (MQ=255)
                                                        ccgTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTa                                                                                                >  7:185150/1‑132 (MQ=255)
                                                        ccgTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTa                                                                                                <  8:185150/132‑1 (MQ=255)
                                                                  aGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCAc                                                                       >  1:254326/1‑147 (MQ=255)
                                                                           caCGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTgg                                                             >  1:221973/1‑148 (MQ=255)
                                                                            aCGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCAGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGc                                                            >  6:373765/1‑148 (MQ=255)
                                                                               aCCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCg                                                         <  2:254326/148‑1 (MQ=255)
                                                                               aCCGCCATAAAACCATGGATCAATCGGCAGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCg                                                         <  5:373765/148‑1 (MQ=255)
                                                                                     aTAAAACCATGGATCAATCGGCTGAGTCGTCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTTACCCGTTACTTGCATCACCATAATTTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTAcc                                                   <  8:54413/148‑1 (MQ=255)
                                                                                     aTAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTa                                                                                                >  7:8150/1‑103 (MQ=255)
                                                                                     aTAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTa                                                                                                <  8:8150/103‑1 (MQ=255)
                                                                                                             aGGCGGCATAATCACCTGCTGGGTAAAAAGCCAACCTTTGTAACCCGTTACTTGCATCACCAAAAATTTAAAGGGCGTAATGCCGATTTTGAAGTCAACCGCACCACTGATTGGCCCGACTAACGTAACCAGTTGTCCACGGAAATc                            >  3:287285/1‑147 (MQ=255)
                                                                                                                     tAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTg                                                                  <  7:312190/101‑1 (MQ=255)
                                                                                                                     tAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTg                                                                  >  8:312190/1‑101 (MQ=255)
                                                                                                                      aaTCACCTTCTTGGTTAAATGCCAACGTTTGTTACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTTGCCCGTCTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTc                   <  2:311772/147‑1 (MQ=255)
                                                                                                                      aaTCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGt                                                                   >  2:61802/1‑99 (MQ=255)
                                                                                                                      aaTCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGt                                                                   <  1:61802/99‑1 (MQ=255)
                                                                                                                      aaTCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAAc                                              >  7:253164/1‑120 (MQ=255)
                                                                                                                      aaTCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAAc                                              <  8:253164/120‑1 (MQ=255)
                                                                                                                      aaTCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGtt                                         <  8:369522/125‑1 (MQ=255)
                                                                                                                      aaTCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGtt                                         >  7:369522/1‑125 (MQ=255)
                                                                                                                      aaTCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTc                   >  1:167890/1‑147 (MQ=255)
                                                                                                                      aaTCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTc                   >  7:193546/1‑147 (MQ=255)
                                                                                                                      aaTCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTc                   >  8:195601/1‑147 (MQ=255)
                                                                                                                      aaTCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTc                   >  2:178683/1‑147 (MQ=255)
                                                                                                                      aaTCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGAACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTc                   >  1:311772/1‑147 (MQ=255)
                                                                                                                       aTCACCTGCTGGGTTAAATGCCAACGTTTGTCACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACAAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCACCCGTGTc                   >  6:117714/1‑146 (MQ=255)
                                                                                                                       aTCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCg                                                         <  7:257892/108‑1 (MQ=255)
                                                                                                                       aTCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCg                                                         >  8:257892/1‑108 (MQ=255)
                                                                                                                       aTCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCg                                                         >  8:251659/1‑108 (MQ=255)
                                                                                                                       aTCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCg                                                         <  7:251659/108‑1 (MQ=255)
                                                                                                                           ccTTCTGGTTTAAATGCAAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCTCTCCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGaaa             <  4:287285/148‑1 (MQ=255)
                                                                                                                                  ggTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTc      <  1:178683/148‑1 (MQ=255)
                                                                                                                                   gTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTca     <  7:195601/148‑1 (MQ=255)
                                                                                                                                    ttAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTca     >  7:156524/1‑147 (MQ=255)
                                                                                                                                      aaaTGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACAt  >  4:24311/1‑148 (MQ=255)
                                                                                                                                          |                                                                                                                                               
ATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACAT  >  NC_000913/1889953‑1890234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑