Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,904,339	C→T	6.0%	L149L (CTG→TTG)	manZ →	mannose‑specific enzyme IID component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,904,339	0	C	T	6.0%	220.3 / 2.7	66	L149L (CTG→TTG)	manZ	mannose‑specific enzyme IID component of PTS
Reads supporting (aligned to +/- strand):  ref base C (32/30);  new base T (2/2);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GACGGTGCTATCAACGGTATCAAAGTCGGTTTGATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGATATGGGTGGTGGCTTCCTGCAAAAACTGACGGAAGGGGCGTCTATCCTCGGCCTGT  >  NC_000913/1904195‑1904486                                                                                                                                                 |                                                                                                                                                    gacgGTGCTATCAACGGTATCAAAGTCGGTTTGATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGt                                                                                                                                                  <  6:111454/148‑1 (MQ=255)           tCAACGGTATCAAAGTCGGTTTGATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCt                                                                                                                                        >  7:237962/1‑148 (MQ=255)            cAACGGTATCAAAGTCGGTTTGATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGttctt                                                                                                                                              >  1:154084/1‑141 (MQ=255)            cAACGGTATCAAAGTCGGTTTGATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGttctt                                                                                                                                              <  2:154084/141‑1 (MQ=255)            cAACGGTATCAAAGTCGGTTTGATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTg                                                                                                                                       >  8:173172/1‑148 (MQ=255)             aaCGGTATCAAAGTCGGTTTGATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGCCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTg                                                                                                                                       <  1:338375/147‑1 (MQ=255)                 gTATCAAAGTCGGTTTGATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTaa                                                                                                                                  <  4:53824/148‑1 (MQ=255)                 gTATCAAAGTCGGTTTGATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTaa                                                                                                                                  <  8:32906/148‑1 (MQ=255)                     cAAAGTCGGTTTGATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGTTGTTCTTCATCCTGTTTAACCTg                                                                                                                              >  7:111035/1‑148 (MQ=255)                     cAAAGTCGGTTTGATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCAt                                                                                                                                           <  4:98857/135‑1 (MQ=255)                     cAAAGTCGGTTTGATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCAt                                                                                                                                           >  3:98857/1‑135 (MQ=255)                     cAAAGTCGGTTTGATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTg                                                                                                                              >  3:218030/1‑148 (MQ=255)                      aaaGTCGGTTTGATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTgg                                                                                                                             >  7:296981/1‑148 (MQ=255)                        aGTCGGTTTGATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGttt                                                                                                                                    >  1:94198/1‑139 (MQ=255)                        aGTCGGTTTGATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGttt                                                                                                                                    <  2:94198/139‑1 (MQ=255)                          tCGGTTTGATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCg                                                                                                                         >  2:189207/1‑148 (MQ=255)                                 gATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTaa                                                                                                                                  >  2:129595/1‑132 (MQ=255)                                 gATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTaa                                                                                                                                  <  1:129595/132‑1 (MQ=255)                                   tGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAAc                                                                                                                >  2:398573/1‑148 (MQ=255)                                     gggACACTGGCTGGTGTAGGCGACCCTATCTTCTGTGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAAccc                                                                                                              <  2:128406/148‑1 (MQ=255)                                        ccACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGTTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGtt                                                                                                           <  8:111035/148‑1 (MQ=255)                                        ccACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGtt                                                                                                           <  1:189207/148‑1 (MQ=255)                                        ccACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGtt                                                                                                           <  8:296981/148‑1 (MQ=255)                                             ggctggTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATcc                                                                                                                                         <  6:188477/113‑1 (MQ=255)                                             ggctggTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATcc                                                                                                                                         >  5:188477/1‑113 (MQ=255)                                             ggctggTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTa                                                                                                                                   <  7:44186/119‑1 (MQ=255)                                             ggctggTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTa                                                                                                                                   >  8:44186/1‑119 (MQ=255)                                             ggctggTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTtactac                                                                                                      >  8:156128/1‑148 (MQ=255)                                                 ggTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGtt                                                                                                           >  4:291059/1‑139 (MQ=255)                                                 ggTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGtt                                                                                                           <  3:291059/139‑1 (MQ=255)                                                  gtgtAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGgcgt                                                                                                 <  7:173172/148‑1 (MQ=255)                                                       ggCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGGTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGgcgtagcgt                                                                                            <  2:87194/148‑1 (MQ=255)                                                         cGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTATGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGACACCCGTTACTACGGCGTAGCGTAt                                                                                          >  8:169615/1‑148 (MQ=255)                                                         cGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTaa                                                                                                                                  <  6:31759/108‑1 (MQ=255)                                                         cGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTaa                                                                                                                                  >  5:31759/1‑108 (MQ=255)                                                         cGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGAGTAGCGTAt                                                                                          >  2:390119/1‑148 (MQ=255)                                                          gACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATg                                                                                         <  8:20270/148‑1 (MQ=255)                                                            cccGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGtt                                                                                                           >  4:2958/1‑128 (MQ=255)                                                            cccGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGtt                                                                                                           <  3:2958/128‑1 (MQ=255)                                                             ccGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGtt                                                                                      <  4:218030/148‑1 (MQ=255)                                                              cGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTa                                                                                     >  5:111590/1‑148 (MQ=255)                                                                 tcttctGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTTTCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTc                                                                                  >  4:98957/1‑148 (MQ=255)                                                                                 aCGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATc                                                                  >  6:226789/1‑148 (MQ=255)                                                                                  cGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATc                                                                  <  1:398573/147‑1 (MQ=255)                                                                                     ccGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGtt                                                               >  6:33005/1‑147 (MQ=255)                                                                                           tttGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTTTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGat                                                         <  5:226789/147‑1 (MQ=255)                                                                                           tttGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGat                                                         <  8:237962/147‑1 (MQ=255)                                                                                           tttGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGat                                                         <  7:156128/147‑1 (MQ=255)                                                                                           tttGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGat                                                         <  3:179084/147‑1 (MQ=255)                                                                                            ttGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGgcgtagcgt                                                                                            <  6:283699/111‑1 (MQ=255)                                                                                            ttGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGgcgtagcgt                                                                                            >  5:283699/1‑111 (MQ=255)                                                                                            ttGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGatat                                                       >  8:395357/1‑148 (MQ=255)                                                                                                agcaCTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGATATGggt                                                   <  5:33005/148‑1 (MQ=255)                                                                                                   aCTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGTTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGATATGggtggt                                                >  3:105690/1‑148 (MQ=255)                                                                                                      gggTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGATATGGGTGGTGGc                                             >  1:206192/1‑148 (MQ=255)                                                                                                      gggTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGATATGGGTGGTGGc                                             >  7:248867/1‑148 (MQ=255)                                                                                                       ggTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGTTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGATATGGGTGGTGGCt                                            <  4:105690/148‑1 (MQ=255)                                                                                                               tATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGATATGGGTGGTGGCTTCCTGCa                                     >  3:318048/1‑147 (MQ=255)                                                                                                                aTCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGATATGGGTGGTGGCTTCCTGCaa                                    >  5:376110/1‑147 (MQ=255)                                                                                                                  cgcgATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGATATGGGTGGTGGCTTCCTGCaaaaa                                 >  7:363674/1‑148 (MQ=255)                                                                                                                   gcgATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGATATGGGTGGTGGCTTCCTGCAAAAAc                                >  2:65158/1‑148 (MQ=255)                                                                                                                     gATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGATATGGGTGGTGGCTTCCTGCAAAAACt                               >  5:210172/1‑147 (MQ=255)                                                                                                                            ggCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGATATGGGTGGTGGCTTCCTGCAAAAACTGACGGAAg                       <  3:98957/148‑1 (MQ=255)                                                                                                                                    gTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATg                                                                                         <  7:274898/74‑1 (MQ=255)                                                                                                                                    gTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATg                                                                                         >  8:274898/1‑74 (MQ=255)                                                                                                                                       aGGTCCGCTGCTGTTCTTCATCCTGGTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGATATGGGTGGTGGCTTCCTGCAAAAACTGACGGAAGGGGCGTCTAt             <  5:333076/147‑1 (MQ=255)                                                                                                                                                 ctgTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGATATGGGTGGTGGCTTCCTGCAAAAACTGACGGAAGGGGCGTCTATCCTCGGCCTGt  <  1:65158/148‑1 (MQ=255)                                                                                                                                                 ctgTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGATATGGGTGGTGGCTTCCTGCAAAAACTGACGGAAGGGGCGTCTATCCTCGGCCTGt  <  6:111590/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                    GACGGTGCTATCAACGGTATCAAAGTCGGTTTGATGGGGCCACTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTATTTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTGCGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGATATGGGTGGTGGCTTCCTGCAAAAACTGACGGAAGGGGCGTCTATCCTCGGCCTGT  >  NC_000913/1904195‑1904486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑