Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,085,179 T→C 6.8% Y116C (TAT→TGT)  yeeE ← UPF0394 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,085,1790TC 6.8% 170.9 / 2.3 58Y116C (TAT→TGT) yeeEUPF0394 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (27/27);  new base C (2/2);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCC  >  NC_000913/2085033‑2085316
                                                                                                                                                  |                                                                                                                                         
gCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCCCCatat                                                                                                                                           >  2:107376/1‑147 (MQ=255)
        aCCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGaaa                                                                                                                                   <  3:263251/147‑1 (MQ=255)
             cAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGcgcg                                                                                                                             <  5:284009/148‑1 (MQ=255)
                   ggCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCaa                                                                                                                       >  4:161006/1‑148 (MQ=255)
                   ggCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCaa                                                                                                                       <  3:161006/148‑1 (MQ=255)
                        ccaacGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAATTGAAAAGAGAGATCCAACTGcc                                                                                                                  >  6:211629/1‑148 (MQ=255)
                          aacGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCg                                                                                                                 >  4:62806/1‑147 (MQ=255)
                          aacGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCg                                                                                                                 <  3:62806/147‑1 (MQ=255)
                             ggCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGa                                                                                                                                     <  4:188390/124‑1 (MQ=255)
                             ggCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGa                                                                                                                                     >  3:188390/1‑124 (MQ=255)
                                                aaGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCCCCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATa                                                                                          <  1:107376/148‑1 (MQ=255)
                                                  gTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATAcc                                                                                        <  6:167589/148‑1 (MQ=255)
                                                      cAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCTCCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGt                                                                                    >  8:398436/1‑148 (MQ=255)
                                                      cAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGt                                                                                    >  6:252139/1‑148 (MQ=255)
                                                            tAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCcgcgcg                                                                                             <  6:129081/133‑1 (MQ=255)
                                                            tAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCcgcgcg                                                                                             >  5:129081/1‑133 (MQ=255)
                                                             agagTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCaa                                                                                                                       >  2:33953/1‑106 (MQ=255)
                                                             agagTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCaa                                                                                                                       <  1:33953/106‑1 (MQ=255)
                                                                      tgcAGTACTGTATTGCTGCAAGGTTTGATTTAAACTACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATACAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCgg                                                                               <  6:103092/135‑1 (MQ=255)
                                                                       tCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATACAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCgg                                                                               <  2:130404/136‑1 (MQ=255)
                                                                       tCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATACAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCgg                                                                               >  1:130404/1‑136 (MQ=255)
                                                                       tCAGTACTGTAGTGCGGCAAGGTTTGATTTAAACCACTGGCATGTGGAGCACGCATCACCGCACTCATCACCATACAAGTGAAACGCGCGAGCCAACTGCCGATCCAGCCCTCACCCGCGCGAGACCAGGTCCCGGt                                                                              >  5:103092/1‑137 (MQ=255)
                                                                               gTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGa                                                                                                                >  7:149465/1‑95 (MQ=255)
                                                                               gTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGa                                                                                                                <  8:149465/95‑1 (MQ=255)
                                                                               gTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGa                                                                                                                >  3:91933/1‑95 (MQ=255)
                                                                               gTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGa                                                                                                                <  4:91933/95‑1 (MQ=255)
                                                                               gTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGa                                                           >  8:119326/1‑148 (MQ=255)
                                                                                  cctctgcAAGCCGTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTCAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACa                                                        <  4:231197/145‑1 (MQ=255)
                                                                                   tgctgcAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCa                                                                                       <  7:52315/116‑1 (MQ=255)
                                                                                    gctgcAAGGTTTGATTTAAACCCCTGTCATGTGGAGCACGCATCACCGCACACATCACCATATAAGTGAAAATAGCGATCCAACTGCCGATCAATCCCTCACCAGCGCGATACCAg                                                                                      >  8:52315/1‑116 (MQ=255)
                                                                                    gctgcAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAAt                                                      >  4:341790/1‑148 (MQ=255)
                                                                                    gctgcAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAAt                                                      >  1:341776/1‑148 (MQ=255)
                                                                                     ctgcAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAAt                                                      <  1:125585/147‑1 (MQ=255)
                                                                                       gcAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACaa                                                       >  3:231197/1‑144 (MQ=255)
                                                                                           ggTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGc                                               >  6:118031/1‑148 (MQ=255)
                                                                                               tGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCg                                            >  7:108087/1‑147 (MQ=255)
                                                                                                gATTTAAACCACTGGCATGTGGAGAACGCATCTCCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGa                                           <  7:398436/147‑1 (MQ=255)
                                                                                                gATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGa                                           <  5:252139/147‑1 (MQ=255)
                                                                                                    tAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGat                                       <  7:119326/147‑1 (MQ=255)
                                                                                                    tAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGat                                       <  3:341790/147‑1 (MQ=255)
                                                                                                               cATGTGGAGAACGCATCACCGCACTCATCACCATATAAGGGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCTCCAGAACAATTCCCAGCCCGAAGATATACCCACCtat                           <  5:172407/148‑1 (MQ=255)
                                                                                                                    ggAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGa                                        <  5:23131/130‑1 (MQ=255)
                                                                                                                    ggAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGa                                        >  6:23131/1‑130 (MQ=255)
                                                                                                                      agaACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGATCAATTCCCAGCCCGAAGATATACCCACCTATAACAGt                     <  5:211629/147‑1 (MQ=255)
                                                                                                                            cATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAAACCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCAGACAGAACAATTCCCAGCCCGAAGATATACCCCCCTATAACACTACCAAGc              >  3:328721/1‑148 (MQ=255)
                                                                                                                               cACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGata                                      >  3:24071/1‑121 (MQ=255)
                                                                                                                               cACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGata                                      <  4:24071/121‑1 (MQ=255)
                                                                                                                               cACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGat                                       <  3:217891/120‑1 (MQ=255)
                                                                                                                               cACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGat                                       >  4:217891/1‑120 (MQ=255)
                                                                                                                               cACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGt                     >  6:342138/1‑138 (MQ=255)
                                                                                                                               cACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGt                     <  5:342138/138‑1 (MQ=255)
                                                                                                                               cACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCAc           >  3:132135/1‑148 (MQ=255)
                                                                                                                                        catcaCCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGcc  >  6:88214/1‑148 (MQ=255)
                                                                                                                                           caCCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACTCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGat                                       >  3:85512/1‑108 (MQ=255)
                                                                                                                                           caCCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACTCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGat                                       <  4:85512/108‑1 (MQ=255)
                                                                                                                                           caCCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGat                                       <  7:3828/108‑1 (MQ=255)
                                                                                                                                           caCCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGat                                       >  8:3828/1‑108 (MQ=255)
                                                                                                                                           caCCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGa       <  3:327053/140‑1 (MQ=255)
                                                                                                                                           caCCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGa       >  4:327053/1‑140 (MQ=255)
                                                                                                                                                 ataAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGat                                       >  7:208095/1‑102 (MQ=255)
                                                                                                                                                 ataAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGat                                       <  8:208095/102‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                         
GCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCC  >  NC_000913/2085033‑2085316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑