Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,120,760 C→T 6.0% P265P (CCG→CCA wcaJ ← colanic biosynthesis UDP‑glucose lipid carrier transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,120,7600CT 6.0% 216.5 / 2.7 66P265P (CCG→CCAwcaJcolanic biosynthesis UDP‑glucose lipid carrier transferase
Reads supporting (aligned to +/- strand):  ref base C (31/31);  new base T (2/2);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

CGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCG  >  NC_000913/2120614‑2120907
                                                                                                                                                  |                                                                                                                                                   
cGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAgcg                                                                                                                                                    <  5:259365/148‑1 (MQ=255)
        gCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCg                                                                                                                                            >  6:258603/1‑148 (MQ=255)
         cGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCg                                                                                                                                            >  1:100683/1‑147 (MQ=255)
               aTAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATAAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCAGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTAGTAAAgag                                                                                                                                     >  4:88595/1‑146 (MQ=255)
                  cccGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGc                                                                                                                                   <  7:244055/146‑1 (MQ=255)
                        ccTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCg                                                                                                                            <  7:175329/148‑1 (MQ=255)
                           ggTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAgcggc                                                                                                                                                  <  1:15590/123‑1 (MQ=255)
                           ggTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAgcggc                                                                                                                                                  >  2:15590/1‑123 (MQ=255)
                           ggTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTa                                                                                                                         >  7:372975/1‑148 (MQ=255)
                           ggTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTa                                                                                                                         >  2:95625/1‑148 (MQ=255)
                            gTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGt                                                                                                                          <  3:195315/146‑1 (MQ=255)
                            gTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGt                                                                                                                          <  2:100683/146‑1 (MQ=255)
                              gAACTGAGTTTCACCGCAAGCGCAATACAGCACAGAACCGGGCAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATTTCTTCCGCACTTTTGACCAGGCGGTTAACACCGCAAAGCGGCGGTTCGTAAAGCGGCACCACCGGTACGc                                                                                                                      >  6:412/1‑148 (MQ=255)
                                aCTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCg                                                                                                                    >  1:247914/1‑148 (MQ=255)
                                 cTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGt                                                                                                                   <  6:151854/148‑1 (MQ=255)
                                       ttCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGt                                                                                                                          >  2:86301/1‑135 (MQ=255)
                                       ttCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGt                                                                                                                          <  1:86301/135‑1 (MQ=255)
                                       ttCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCAtct                                                                                                             >  3:68381/1‑148 (MQ=255)
                                        tCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCAtct                                                                                                             <  5:258603/147‑1 (MQ=255)
                                            cGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCg                                                                                                        <  2:247914/148‑1 (MQ=255)
                                              ccAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAg                                                                                                      <  4:68381/148‑1 (MQ=255)
                                              ccAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAg                                                                                                      <  3:88595/148‑1 (MQ=255)
                                                     aaTACAGCACAGCTCCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGAACAATGTCTTCCGCACGATTGAGCTGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTg                                                                                                 <  5:412/146‑1 (MQ=255)
                                                      aTACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCAt                                                                                                               <  1:335202/131‑1 (MQ=255)
                                                        acagcacagcACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGTGGCGTGTCGTAAAGCGGCACCACCGGt                                                                                                                          >  3:149323/1‑118 (MQ=255)
                                                        acagcacagcACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGTGGCGTGTCGTAAAGCGGCACCACCGGt                                                                                                                          <  4:149323/118‑1 (MQ=255)
                                                        acagcacagcACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATg                                                                                             >  2:55322/1‑147 (MQ=255)
                                                        acagcacagcACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATg                                                                                             >  7:125851/1‑147 (MQ=255)
                                                        acagcacagcACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATg                                                                                             >  7:67354/1‑147 (MQ=255)
                                                                          gaTCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATctct                                                                                                           >  4:277227/1‑115 (MQ=255)
                                                                          gaTCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATctct                                                                                                           <  3:277227/115‑1 (MQ=255)
                                                                          gaTCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTg                                                                                                 >  6:200691/1‑125 (MQ=255)
                                                                          gaTCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTg                                                                                                 <  5:200691/125‑1 (MQ=255)
                                                                            tCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCg                                                                                                        <  1:120877/116‑1 (MQ=255)
                                                                            tCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCg                                                                                                        >  2:120877/1‑116 (MQ=255)
                                                                            tCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGa                                                                                                >  4:19633/1‑124 (MQ=255)
                                                                            tCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGa                                                                                                <  3:19633/124‑1 (MQ=255)
                                                                            tCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGa                                                                                                <  7:203593/124‑1 (MQ=255)
                                                                            tCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGa                                                                                                >  8:203593/1‑124 (MQ=255)
                                                                            tCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTaa                                                                            >  4:310727/1‑144 (MQ=255)
                                                                            tCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTaa                                                                            <  3:310727/144‑1 (MQ=255)
                                                                                 agcagAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCgg                                                                   >  5:101740/1‑148 (MQ=255)
                                                                                 agcagAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCgg                                                                   >  5:295452/1‑148 (MQ=255)
                                                                                   cagAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGt                                                                                                                          <  6:123339/91‑1 (MQ=255)
                                                                                   cagAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGt                                                                                                                          >  5:123339/1‑91 (MQ=255)
                                                                                      aaTAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGAt                                                               <  4:302325/147‑1 (MQ=255)
                                                                                      aaTAAGCGTCGCAAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGAt                                                               >  3:302325/1‑147 (MQ=255)
                                                                                         aaGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGa                                                                                                <  3:176670/111‑1 (MQ=255)
                                                                                         aaGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGa                                                                                                >  4:176670/1‑111 (MQ=255)
                                                                                          aGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATcagc                                                           <  1:55322/147‑1 (MQ=255)
                                                                                               cGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGa                                                                                                <  4:7481/105‑1 (MQ=255)
                                                                                               cGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGa                                                                                                >  3:7481/1‑105 (MQ=255)
                                                                                                     cacaATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAg                                               >  8:210824/1‑148 (MQ=255)
                                                                                                      acaATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAgg                                              <  8:125851/148‑1 (MQ=255)
                                                                                                       caATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAggt                                             <  7:362138/148‑1 (MQ=255)
                                                                                                        aaTGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCgggg                                                                 <  1:321724/127‑1 (MQ=255)
                                                                                                        aaTGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCgggg                                                                 >  2:321724/1‑127 (MQ=255)
                                                                                                           gTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGTGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCGGCAGCACCGAACAGgtggtg                                         >  7:137682/1‑148 (MQ=255)
                                                                                                            tCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGgtgt                                        <  6:101740/148‑1 (MQ=255)
                                                                                                                   cACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCg                                                                                                        >  5:132237/1‑77 (MQ=255)
                                                                                                                   cACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCg                                                                                                        <  6:132237/77‑1 (MQ=255)
                                                                                                                   cACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGgaga                                                                                         >  6:47959/1‑92 (MQ=255)
                                                                                                                   cACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGgaga                                                                                         <  5:47959/92‑1 (MQ=255)
                                                                                                                      gTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTg                              <  1:95625/148‑1 (MQ=255)
                                                                                                                                 cGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGAGCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGtt                   >  3:11699/1‑148 (MQ=255)
                                                                                                                                    ttAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACTCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGttttt                >  4:171193/1‑148 (MQ=255)
                                                                                                                                       aCCCCGGAAAGTGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCGGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTc              <  8:137682/147‑1 (MQ=255)
                                                                                                                                        ccccGGAAAGCGGCGTGTCGTAAAGCGGCTCCACCGGTACGCCGGTGATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAAGAGGTGGGGTCCGCCAGTTGATGGCCCAGtttgttacc            >  2:60179/1‑145 (MQ=255)
                                                                                                                                                aGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCgcgcgc    <  6:295452/148‑1 (MQ=255)
                                                                                                                                                  cggcgTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCg  <  1:60179/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCG  >  NC_000913/2120614‑2120907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: