Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,413,461 G→A 100% intergenic (‑329/‑9) yfbV ← / → ackA UPF0208 family inner membrane protein/acetate kinase A and propionate kinase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,413,4610GA100.0% 331.5 / ‑6.7 93intergenic (‑329/‑9)yfbV/ackAUPF0208 family inner membrane protein/acetate kinase A and propionate kinase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (41/51);  minor base T (0/1);  total (41/52)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCAAA  >  NC_000913/2413316‑2413604
                                                                                                                                                 |                                                                                                                                               
aTTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGt                                                                                                                                               <  5:270502/148‑1 (MQ=255)
      cAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTcc                                                                                                                                         <  5:372070/148‑1 (MQ=255)
      cAAAATGGCATAGACTCAAGATATTTCTTCCATCATGAAAAAAAAATTTGCAGTTCATGATGTTAATCATAAATGTCGGTGTCATCATGCTCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTcc                                                                                                                                         <  7:171767/148‑1 (MQ=255)
        aaaTGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGt                                                                                                                                               >  8:401369/1‑140 (MQ=255)
        aaaTGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGt                                                                                                                                               <  7:401369/140‑1 (MQ=255)
         aaTGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTcc                                                                                                                                         <  1:121566/145‑1 (MQ=255)
         aaTGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTcc                                                                                                                                         >  2:121566/1‑145 (MQ=255)
         aaTGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATg                                                                                                                                      >  3:282274/1‑148 (MQ=255)
         aaTGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATg                                                                                                                                      >  3:277359/1‑148 (MQ=255)
          aTGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGt                                                                                                                                     <  3:236196/148‑1 (MQ=255)
             gCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGa                                                                                                                                  <  4:49195/148‑1 (MQ=255)
               aTAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGt                                                                                                                                <  4:46293/148‑1 (MQ=255)
                tAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGt                                                                                                                                >  4:336755/1‑147 (MQ=255)
                 aGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGt                                                                                                                                               <  7:138006/131‑1 (MQ=255)
                 aGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGt                                                                                                                                               >  8:138006/1‑131 (MQ=255)
                 aGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTaa                                                                                                                              >  4:74266/1‑148 (MQ=255)
                    cTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGt                                                                                                                            >  8:288531/1‑147 (MQ=255)
                      cAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAg                                                                                                                         >  8:18096/1‑148 (MQ=255)
                       aatatatTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGTGCTAATCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAg                                                                                                                         <  3:277907/144‑1 (MQ=255)
                       aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAg                                                                                                                         >  4:146001/1‑147 (MQ=255)
                       aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGt                                                                                                                        <  4:99270/148‑1 (MQ=255)
                       aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGt                                                                                                                        <  2:364269/148‑1 (MQ=255)
                       aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGt                                                                                                                        <  4:282274/148‑1 (MQ=255)
                       aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGt                                                                                                                        <  4:277359/148‑1 (MQ=255)
                       aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGt                                                                                                                        <  3:336755/148‑1 (MQ=255)
                       aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGt                                                                                                                        <  2:247422/148‑1 (MQ=255)
                         gATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTAc                                                                                                                      >  4:23717/1‑148 (MQ=255)
                          atatTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACt                                                                                                                     >  7:89592/1‑148 (MQ=255)
                            atTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTgg                                                                                                                   >  4:181047/1‑148 (MQ=255)
                            atTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGACGAGTaa                                                                                                                              <  8:307157/137‑1 (MQ=255)
                            atTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGACGAGTaa                                                                                                                              >  7:307157/1‑137 (MQ=255)
                              ttcttcCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTGCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTcc                                                                                                                                         >  3:134750/1‑124 (MQ=255)
                              ttcttcCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTGCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTcc                                                                                                                                         >  3:129541/1‑124 (MQ=255)
                              ttcttcCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTcc                                                                                                                                         <  4:129541/124‑1 (MQ=255)
                              ttcttcCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTcc                                                                                                                                         <  4:134750/124‑1 (MQ=255)
                               tcttcCATCATGCAAAAAAAATTTCCAGTGCATGATGTTAATCATAAATGTCGGTGGCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGtt                                                                                                                 <  6:367825/147‑1 (MQ=255)
                                   ccATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGa                                                                                                             <  3:146001/147‑1 (MQ=255)
                                                  aaTTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGttcttc                                                                                             <  3:74266/148‑1 (MQ=255)
                                                          gTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAAt                                                                                     <  7:18096/148‑1 (MQ=255)
                                                            gCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACTCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTATTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAAttt                                                                                   >  6:156390/1‑148 (MQ=255)
                                                            gCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCg                                                                                                                                   >  8:284507/1‑100 (MQ=255)
                                                            gCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCg                                                                                                                                   <  7:284507/100‑1 (MQ=255)
                                                            gCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAAttt                                                                                   >  3:112800/1‑148 (MQ=255)
                                                            gCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAAttt                                                                                   >  6:166231/1‑148 (MQ=255)
                                                            gCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAAttt                                                                                   >  4:300232/1‑148 (MQ=255)
                                                             cATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTg                                                                                  >  7:205261/1‑148 (MQ=255)
                                                                gatgTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCca                                                                               >  7:99574/1‑148 (MQ=255)
                                                                 atgTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCcat                                                                              >  5:7715/1‑148 (MQ=255)
                                                                 atgTTAATCATAAATGTCGGTGTCACCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAAt                                                                                     >  3:91739/1‑141 (MQ=255)
                                                                 atgTTAATCATAAATGTCGGTGTCACCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAAt                                                                                     <  4:91739/141‑1 (MQ=255)
                                                                   gTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTAAAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCATAACTGCGGTAGTTCTTCACTGAAATTTGCcataa                                                                            >  6:129523/1‑146 (MQ=255)
                                                                    ttAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCcatcat                                                                           >  8:361272/1‑148 (MQ=255)
                                                                     tAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCatcatc                                                                          >  6:199218/1‑148 (MQ=255)
                                                                       aTCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCg                                                                         <  3:181047/147‑1 (MQ=255)
                                                                       aTCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCg                                                                         <  1:111313/147‑1 (MQ=255)
                                                                       aTCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCg                                                                         <  4:112800/147‑1 (MQ=255)
                                                                          aTAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGc                                                                                                        >  3:145766/1‑113 (MQ=255)
                                                                          aTAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGc                                                                                                        <  4:145766/113‑1 (MQ=255)
                                                                           tAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATg                                                                      >  3:32761/1‑146 (MQ=255)
                                                                            aaaTGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAg                                                                   >  3:282100/1‑148 (MQ=255)
                                                                               tGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTaa                                                                <  3:300232/148‑1 (MQ=255)
                                                                               tGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTaa                                                                <  4:32761/148‑1 (MQ=255)
                                                                                     tgtCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTg                                                          <  8:99574/148‑1 (MQ=255)
                                                                                     tgtCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTg                                                          <  4:282100/148‑1 (MQ=255)
                                                                                      gtCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTaa                                                                                                                              <  2:80017/79‑1 (MQ=255)
                                                                                      gtCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTaa                                                                                                                              >  1:80017/1‑79 (MQ=255)
                                                                                       tcatcaTGCGCTACGCTCCATGGCTCCCTGACTTTGTTTTATCCACGTATCAATTATAAGTACTTCCATTTCGAGTAAGTTAGTCCTGGTTCTGACCTGCGGTAGTTCTTCCCTGAAATTTGCCAGCATCGATGCAGTAAATGGTGa                                                         <  8:89592/147‑1 (MQ=255)
                                                                                        catcatGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGt                                                                                                                        <  3:73028/83‑1 (MQ=255)
                                                                                        catcatGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGt                                                                                                                        >  4:73028/1‑83 (MQ=255)
                                                                                         atcatGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAaga                                                      <  7:288531/148‑1 (MQ=255)
                                                                                           catGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAag                                                       >  3:240603/1‑145 (MQ=255)
                                                                                           catGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAag                                                       <  4:240603/145‑1 (MQ=255)
                                                                                                   aCGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGttctt                                                                                              >  2:289623/1‑98 (MQ=255)
                                                                                                   aCGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGttctt                                                                                              <  1:289623/98‑1 (MQ=255)
                                                                                                    cGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCt                                            <  3:23717/147‑1 (MQ=255)
                                                                                                    cGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTg                                           >  8:150623/1‑148 (MQ=255)
                                                                                                     gCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTgg                                          <  7:361272/148‑1 (MQ=255)
                                                                                                                    gACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACAGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGAAGCAGTAAATGTTGAAGATTACCTTTCTGGTTTAGCCTAATGttt                           >  6:22006/1‑148 (MQ=255)
                                                                                                                      cGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTcc                         <  7:140004/148‑1 (MQ=255)
                                                                                                                        tttttttAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCAc                       <  5:199218/148‑1 (MQ=255)
                                                                                                                        tttttttAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCAc                       <  7:150623/148‑1 (MQ=255)
                                                                                                                                gacACGTTTCAATTATATGTACTTCCATGTCGAGTAAGTTAGTACTGTTTCTGAACTGCGGTAGTTCTTCACTGTAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGa               <  5:22006/146‑1 (MQ=255)
                                                                                                                                    cGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCa           <  6:7715/148‑1 (MQ=255)
                                                                                                                                     gTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGt                             <  4:202170/129‑1 (MQ=255)
                                                                                                                                     gTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGt                             >  3:202170/1‑129 (MQ=255)
                                                                                                                                     gTATCAATTATAAGTACTTCCATGTCAAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTcc                         >  6:344512/1‑133 (MQ=255)
                                                                                                                                     gTATCAATTATAAGTACTTCCATGTCAAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTcc                         <  5:344512/133‑1 (MQ=255)
                                                                                                                                      tATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTGCTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACg         >  1:175568/1‑148 (MQ=255)
                                                                                                                                         cAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGCTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTAt      >  7:47526/1‑148 (MQ=255)
                                                                                                                                            ttATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCaa   <  5:129523/148‑1 (MQ=255)
                                                                                                                                            ttATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCaa   <  5:166231/148‑1 (MQ=255)
                                                                                                                                            ttATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCaa   <  8:47526/148‑1 (MQ=255)
                                                                                                                                              ataAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCaaa  <  5:156390/147‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
ATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCAAA  >  NC_000913/2413316‑2413604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: