Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,469,009 G→T 100% V266F (GTT→TTT)  gtrB → CPS‑53 (KpLE1) prophage; bactoprenol glucosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,469,0090GT99.2% 466.6 / ‑3.8 129coding (796/921 nt)gtrBCPS‑53 (KpLE1) prophage; bactoprenol glucosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (75/53);  minor base . (0/1);  total (75/54)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 131 total aligned reads displayed.

TACAAGCTTTTCCACATTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTGTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTAT  >  NC_000913/2468867‑2469152
                                                                                                                                              |                                                                                                                                               
tACAAGCTTTTCCACATTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTc                                                                                                                                             >  3:8735/1‑147 (MQ=255)
  cAAGCTTTTCCACATTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTtttt                                                                                                                                               <  1:236434/143‑1 (MQ=255)
                       ttCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAAtt                                                                                                                       >  5:371455/1‑146 (MQ=255)
                       ttCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAAtt                                                                                                                       >  5:51394/1‑146 (MQ=255)
                              cTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTTGAATTGAGATGAtt                                                                                                              >  3:307002/1‑148 (MQ=255)
                              cTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGAtt                                                                                                              >  4:238998/1‑148 (MQ=255)
                               tGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTg                                                                                                             >  1:170432/1‑148 (MQ=255)
                                   catacataGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAAt                                                                                                         <  1:169928/148‑1 (MQ=255)
                                         taGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGt                                                                                                   <  4:8735/148‑1 (MQ=255)
                                           gggTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTa                                                                                                  <  3:157536/147‑1 (MQ=255)
                                           gggTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTAt                                                                                                 >  7:131021/1‑148 (MQ=255)
                                           gggTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTAt                                                                                                 <  6:371455/148‑1 (MQ=255)
                                            ggTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGttttt                                                                                                                                 >  2:283252/1‑115 (MQ=255)
                                              ttAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAg                                                                                              <  2:145986/148‑1 (MQ=255)
                                                         cAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATAtt                                                                                   <  3:149294/148‑1 (MQ=255)
                                                           gtgtAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTgg                                                                                 <  3:238998/148‑1 (MQ=255)
                                                             gtAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTAtt                                                                                                <  1:219106/131‑1 (MQ=255)
                                                             gtAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTAtt                                                                                                >  2:219106/1‑131 (MQ=255)
                                                             gtAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGAc                                                                               >  2:158770/1‑148 (MQ=255)
                                                             gtAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGAc                                                                               >  2:92572/1‑148 (MQ=255)
                                                             gtAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGAc                                                                               >  5:202987/1‑148 (MQ=255)
                                                             gtAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGAc                                                                               >  2:264026/1‑148 (MQ=255)
                                                               aGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAAt                                                                                                                        <  6:123943/105‑1 (MQ=255)
                                                               aGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGATTAGGAGTATTAGGTGAATATATTGGACg                                                                              >  2:157536/1‑147 (MQ=255)
                                                               aGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAatat                                                                                      >  8:121095/1‑139 (MQ=255)
                                                               aGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAatat                                                                                      <  7:121095/139‑1 (MQ=255)
                                                               aGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAAGAGGAGTAATAGGTGAATATATTGGACg                                                                              >  6:331884/1‑147 (MQ=255)
                                                                 cATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGcac                                                                           <  4:352625/148‑1 (MQ=255)
                                                                  atttatttATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGcac                                                                           <  2:175474/147‑1 (MQ=255)
                                                                   tttatttaTGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGcaca                                                                          >  4:244581/1‑147 (MQ=255)
                                                                   tttatttaTGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGAAGcacc                                                                          >  4:144139/1‑146 (MQ=255)
                                                                    ttatttatGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCacata                                                                        >  6:139805/1‑148 (MQ=255)
                                                                    ttatttatGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCacata                                                                        >  5:28969/1‑148 (MQ=255)
                                                                    ttatttatGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCacata                                                                        >  5:170966/1‑148 (MQ=255)
                                                                     tatttatGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTa                                                                                                                                <  2:21276/91‑1 (MQ=255)
                                                                     tatttatGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTa                                                                                                                                >  1:21276/1‑91 (MQ=255)
                                                                     tatttatGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCacata                                                                        <  1:264026/147‑1 (MQ=255)
                                                                     tatttatGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCacata                                                                        >  8:251305/1‑147 (MQ=255)
                                                                      atttatGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCacatac                                                                       <  6:202987/147‑1 (MQ=255)
                                                                       tttatGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCacataca                                                                      <  1:158770/147‑1 (MQ=255)
                                                                           tGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGaa                                                                 <  3:159602/148‑1 (MQ=255)
                                                                            ggggCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGaaa                                                                >  3:231326/1‑148 (MQ=255)
                                                                             gggCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGaaa                                                                <  2:170432/147‑1 (MQ=255)
                                                                               gCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTGAGGGGATATCCTTCACTACTTTTTTCACTACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAAcc                                                              >  2:374556/1‑147 (MQ=255)
                                                                                  tGGATGATTTTAGATACTATCAAATTTGGAAATGCTGTTAGGTGAGATCATTCATTACTTTTTTCATTACTGTTTTTAGTTGGAATTAAGATGATTGGAATAGTAGTATTATGTGAATATATTGTACGCacataca                                                                      <  2:247760/136‑1 (MQ=255)
                                                                                   ggATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGTTATATCCTTCAGTACTTTTTTAAATAATGTTTTTAGGTTGAATTCAGATGATTGGAATAGCAGTATTAGGTGAATATATTGGACGCaaatacat                                                                     >  1:247760/1‑136 (MQ=255)
                                                                                   ggATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGca                                                                            >  2:235381/1‑129 (MQ=255)
                                                                                   ggATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCacat                                                                         <  4:88212/132‑1 (MQ=255)
                                                                                   ggATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCacat                                                                         >  3:88212/1‑132 (MQ=255)
                                                                                   ggATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCaaaa                                                          >  6:39403/1‑147 (MQ=255)
                                                                                   ggATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCaaaa                                                          >  8:398322/1‑147 (MQ=255)
                                                                                   ggATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCaaaa                                                          >  1:363931/1‑147 (MQ=255)
                                                                                   ggATGAGTTTAGATACTATCATTTTTGGAAATGCTGTTAGGGGAGATCCTTGACTACTTTTTTCAATACTGGTGTTAGGTGGAAGTCTGATGATTGGAATAg                                                                                                       >  2:268453/1‑102 (MQ=255)
                                                                                    gatgatTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTAtt                                                                                                <  1:384823/108‑1 (MQ=255)
                                                                                    gatgatTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCaaaaa                                                         <  4:231326/147‑1 (MQ=255)
                                                                                    gatgatTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCaaaaa                                                         <  3:345386/147‑1 (MQ=255)
                                                                                    gatgatTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCaaaaa                                                         <  6:28969/147‑1 (MQ=255)
                                                                                    gatgatTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCaaaaa                                                         <  2:363931/147‑1 (MQ=255)
                                                                                      tgatTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTTGGTGAATATATTGGACGCACATACATTGAAACCAAAATACg                                                      >  1:312000/1‑148 (MQ=255)
                                                                                        atTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGc                                                     <  3:236282/147‑1 (MQ=255)
                                                                                          tttAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTg                                                                   <  6:229110/131‑1 (MQ=255)
                                                                                          tttAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTg                                                                   >  5:229110/1‑131 (MQ=255)
                                                                                                tACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATa                                             >  5:318857/1‑147 (MQ=255)
                                                                                                    aTCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATATTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATAcatc                                         >  6:253239/1‑147 (MQ=255)
                                                                                                    aTCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCaaaaa                                                         <  1:11698/131‑1 (MQ=255)
                                                                                                    aTCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCaaaa                                                          <  1:228116/130‑1 (MQ=255)
                                                                                                    aTCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCaaaa                                                          >  2:228116/1‑130 (MQ=255)
                                                                                                    aTCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATAcatca                                        >  5:224975/1‑148 (MQ=255)
                                                                                                    aTCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATAcatca                                        >  7:265140/1‑148 (MQ=255)
                                                                                                       atatTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACatcatc                                      <  6:318857/147‑1 (MQ=255)
                                                                                                         atTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGcac                                                                           <  2:90551/108‑1 (MQ=255)
                                                                                                         atTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGcac                                                                           >  1:90551/1‑108 (MQ=255)
                                                                                                         atTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGaaa                                                                >  5:115793/1‑119 (MQ=255)
                                                                                                         atTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACatcatc                                      >  7:198405/1‑145 (MQ=255)
                                                                                                          tttGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAag                                   <  5:39403/147‑1 (MQ=255)
                                                                                                          tttGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAag                                   <  5:139805/147‑1 (MQ=255)
                                                                                                                   gCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCaaaaa                                                         <  8:145635/116‑1 (MQ=255)
                                                                                                                   gCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCaaaaa                                                         >  7:145635/1‑116 (MQ=255)
                                                                                                                   gCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCaaa                          >  3:329028/1‑147 (MQ=255)
                                                                                                                     tGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCaaaaaa                       >  4:223511/1‑148 (MQ=255)
                                                                                                                       tacgggtATATCATACAATACTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATg                     <  6:374281/144‑1 (MQ=255)
                                                                                                                        tAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAAt                                              >  5:307051/1‑122 (MQ=255)
                                                                                                                        tAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAAt                                              <  6:307051/122‑1 (MQ=255)
                                                                                                                               tatCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGa                    >  4:288196/1‑141 (MQ=255)
                                                                                                                               tatCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGa                    <  3:288196/141‑1 (MQ=255)
                                                                                                                                atCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAga             >  5:320358/1‑146 (MQ=255)
                                                                                                                                 tCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCaa           <  7:398322/148‑1 (MQ=255)
                                                                                                                                  ccTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCaa           >  8:132124/1‑147 (MQ=255)
                                                                                                                                     tCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATaa        >  8:141816/1‑147 (MQ=255)
                                                                                                                                      cACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATAca                                           <  4:218664/111‑1 (MQ=255)
                                                                                                                                      cACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATaaa       <  1:92572/147‑1 (MQ=255)
                                                                                                                                      cACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATaa        >  6:68346/1‑146 (MQ=255)
                                                                                                                                         tactTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCaaaaaa                       >  2:291716/1‑128 (MQ=255)
                                                                                                                                         tactTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCaaaaaa                       <  1:291716/128‑1 (MQ=255)
                                                                                                                                         tactTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTa   >  6:133725/1‑148 (MQ=255)
                                                                                                                                         tactTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTa   >  7:211219/1‑148 (MQ=255)
                                                                                                                                          actTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCaa                                    <  1:85695/114‑1 (MQ=255)
                                                                                                                                          actTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCaa                                    >  2:85695/1‑114 (MQ=255)
                                                                                                                                          actTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGAAATAAAAGTa   >  7:52406/1‑147 (MQ=255)
                                                                                                                                           ctTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTAt  >  8:290515/1‑147 (MQ=255)
                                                                                                                                              |                                                                                                                                               
TACAAGCTTTTCCACATTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTGTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTAT  >  NC_000913/2468867‑2469152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: