| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,538,798 | T→C | 8.0% | G262G (GGA→GGG) | cysM ← | cysteine synthase B (O‑acetylserine sulfhydrolase B) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,538,798 | 0 | T | C | 8.0% | 148.6 / 3.3 | 50 | G262G (GGA→GGG) | cysM | cysteine synthase B (O‑acetylserine sulfhydrolase B) |
| Reads supporting (aligned to +/- strand): ref base T (22/24); new base C (2/2); total (24/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGATGCTATTAATCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGT > NC_000913/2538656‑2538944 | ccGATGCTATTAATCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGc > 2:88512/1‑148 (MQ=255) cGATGCTATTAATCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCa < 1:316350/148‑1 (MQ=255) aTGCTATTAATCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAAc > 7:220961/1‑148 (MQ=255) ttAATCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACcgcg < 3:87177/146‑1 (MQ=255) aaTCGTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGcgccgc > 7:187142/1‑148 (MQ=255) aaTCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGcgccgc > 3:293436/1‑148 (MQ=255) aaTCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTg < 4:293436/148‑1 (MQ=255) tCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGAc > 6:383552/1‑148 (MQ=255) cTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAat < 8:375535/148‑1 (MQ=255) cTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAat < 1:88512/148‑1 (MQ=255) tGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTcc < 4:198284/113‑1 (MQ=255) tGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTcc > 3:198284/1‑113 (MQ=255) ggCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAatat > 6:254612/1‑148 (MQ=255) gCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAatat < 8:220961/147‑1 (MQ=255) ctTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCAc > 7:369653/1‑148 (MQ=255) tCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAat < 2:242827/132‑1 (MQ=255) tCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAat > 1:242827/1‑132 (MQ=255) ccccAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCCCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGc > 7:95954/1‑148 (MQ=255) aaaCACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCCCCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGt > 2:278738/1‑148 (MQ=255) cccGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCgcgc < 5:254612/148‑1 (MQ=255) ggTAGAAAGGTAGCGATCGCCACGATCGCAGATGCTCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATg > 2:105288/1‑148 (MQ=255) aGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCg > 1:213702/1‑147 (MQ=255) gATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGACACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATc < 8:187142/148‑1 (MQ=255) tCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCCCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCgcgc < 3:161608/129‑1 (MQ=255) tCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCCCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCgcgc > 4:161608/1‑129 (MQ=255) cGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCgcgc > 7:269427/1‑128 (MQ=255) cGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCgcgc < 8:269427/128‑1 (MQ=255) cACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCtg < 8:369653/148‑1 (MQ=255) gATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCCCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCtgatg < 8:95954/148‑1 (MQ=255) tCGCAGATGATCGCCACCCCCACCGCGTCAGGCTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGATGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCAGCGCGCTGATGaa < 2:264553/148‑1 (MQ=255) gatgatCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATcc < 5:383552/148‑1 (MQ=255) atCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATCCAGc < 5:279484/147‑1 (MQ=255) caccGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATc < 1:226659/132‑1 (MQ=255) caccGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATc > 2:226659/1‑132 (MQ=255) gcgTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCtg < 7:65100/119‑1 (MQ=255) gcgTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCtg > 8:65100/1‑119 (MQ=255) cAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCgcgc < 8:108245/92‑1 (MQ=255) cAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCgcgc > 7:108245/1‑92 (MQ=255) cAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCgcgc > 6:357349/1‑92 (MQ=255) cAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCgcgc < 5:357349/92‑1 (MQ=255) gTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATCCAGCACCTCATCCACCagag > 2:389210/1‑140 (MQ=255) gTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATCCAGCACCTCATCCACCagag < 1:389210/140‑1 (MQ=255) aGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCtg > 4:136992/1‑108 (MQ=255) aGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCtg < 3:136992/108‑1 (MQ=255) aGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGaa > 8:310848/1‑147 (MQ=255) tGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATcccc > 6:257369/1‑148 (MQ=255) gCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATcc > 2:55086/1‑99 (MQ=255) gCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATcc < 1:55086/99‑1 (MQ=255) cTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGt < 5:257369/148‑1 (MQ=255) cTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGt < 2:213702/148‑1 (MQ=255) | CCGATGCTATTAATCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGT > NC_000913/2538656‑2538944 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |