Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,159,199	G→T	G444V (GGC→GTC)	ptsG →	fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,159,199	0	G	T	100.0%	159.0 / NA	49	G444V (GGC→GTC)	ptsG	fused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/25);  total (24/25)

GTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGAT  >  NC_000913/1159058‑1159337                                                                                                                                              |                                                                                                                                           gTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCg                                                                                                                                     >  1:35718/1‑149 (MQ=255)            aTGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGt                                                                                                                          <  4:116756/149‑1 (MQ=255)              ggCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGGATGTATTACCCGTCTGAGCGTCAGCGTTGCTGAGGTGTCTAATGTGGATCAGGACGGCCTGAAGAAACTGGTCGAAGCGGGCGGAGTGGTTg                                                                                                                        >  8:116015/1‑149 (MQ=255)                cACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCt                                                                                                                      >  1:169299/1‑149 (MQ=255)                 aCCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTg                                                                                                                     >  3:55993/1‑149 (MQ=255)                    ggCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGtt                                                                                                                  <  4:68445/149‑1 (MQ=255)                         tGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGAGGTTGCTGGTTCTGgt                                                                                                             >  3:79244/1‑149 (MQ=255)                             tgctgcATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTc                                                                                                         <  7:3146/149‑1 (MQ=255)                              gctgcATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTc                                                                                                         <  2:169299/148‑1 (MQ=255)                               ctgcATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAg                                                                                                       >  5:105498/1‑149 (MQ=255)                                         tggtAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGtcg                                                                                                                                          <  8:21735/104‑1 (MQ=255)                                              aaGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCa                                                                                                        <  4:86607/133‑1 (MQ=255)                                              aaGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCa                                                                                                        >  3:86607/1‑133 (MQ=255)                                                gAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTa                                                                                       >  2:26594/1‑148 (MQ=255)                                                gAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTa                                                                                       >  6:80113/1‑148 (MQ=255)                                                       ttACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGTGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGAt                                                                               <  7:116015/149‑1 (MQ=255)                                                           tAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCg                                                                                             <  6:107278/131‑1 (MQ=255)                                                           tAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCg                                                                                             >  5:107278/1‑131 (MQ=255)                                                             aCCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAAcc                                                                           <  6:3857/147‑1 (MQ=255)                                                             aCCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCt                                                                          <  2:35718/148‑1 (MQ=255)                                                                            ttACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgatggat                                                          <  1:26594/149‑1 (MQ=255)                                                                             tACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATc                                                                                   >  8:106092/1‑123 (MQ=255)                                                                             tACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATc                                                                                   <  7:106092/123‑1 (MQ=255)                                                                              aCCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgatggat                                                          >  7:143074/1‑147 (MQ=255)                                                                               cccGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAg                                                       <  8:16777/149‑1 (MQ=255)                                                                               cccGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGAGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAg                                                       <  4:79244/149‑1 (MQ=255)                                                                                ccGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGa                                                        <  5:109632/147‑1 (MQ=255)                                                                                ccGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGa                                                        >  6:109632/1‑147 (MQ=255)                                                                                ccGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAg                                                       >  3:38562/1‑148 (MQ=255)                                                                                       cgcgTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGGTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCg                                               >  6:133002/1‑149 (MQ=255)                                                                                        gcgTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATcc                                                                                  >  2:98200/1‑113 (MQ=255)                                                                                        gcgTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATcc                                                                                  <  1:98200/113‑1 (MQ=255)                                                                                               cGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACAt                                                  >  2:35547/1‑138 (MQ=255)                                                                                               cGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACAt                                                  <  1:35547/138‑1 (MQ=255)                                                                                               cGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACt                                       <  5:80113/149‑1 (MQ=255)                                                                                                       aTGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGt                                                      >  3:12210/1‑126 (MQ=255)                                                                                                       aTGTGTCTAAAGTGCATCAGTCCGGCCTGAAGAAACTGCTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCCGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGt                                                      <  4:12210/126‑1 (MQ=255)                                                                                                         gtgtCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGt                                >  5:52790/1‑146 (MQ=255)                                                                                                         gtgtCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTaa                              <  6:52790/148‑1 (MQ=255)                                                                                                           gtCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGc                                                                                                     >  8:18179/1‑75 (MQ=255)                                                                                                           gtCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGc                                                                                                     <  7:18179/75‑1 (MQ=255)                                                                                                                 aGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTggg                     <  6:105498/149‑1 (MQ=255)                                                                                                                     gATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATcc                                  >  5:39081/1‑132 (MQ=255)                                                                                                                      aTCAGGCCGGCCGGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGGGGTCAGGCGATTGTCGGGACGAAAGCCGATAACCTGACAACCGAGAGGGATGAGTACATCCGTACCCACTAATCCGTAAGCCGTTGGGgagcc                >  3:38899/1‑147 (MQ=255)                                                                                                                           gccggccTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGt                                              <  6:28832/114‑1 (MQ=255)                                                                                                                               gccTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGc       <  8:143074/149‑1 (MQ=255)                                                                                                                                    aagaaACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGAt  <  7:53213/149‑1 (MQ=255)                                                                                                                                    aagaaACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGAt  >  8:53213/1‑149 (MQ=255)                                                                                                                                          cTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGc          <  6:171056/135‑1 (MQ=255)                                                                                                                                          cTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGc          >  5:171056/1‑135 (MQ=255)                                                                                                                                              |                                                                                                                                           GTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGAT  >  NC_000913/1159058‑1159337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑