Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,158,626 G→A 100% G253D (GGC→GAC)  ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,158,6260GA100.0% 242.6 / ‑6.7 72G253D (GGC→GAC) ptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (33/38);  minor base C (1/0);  total (34/38)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGAGCCGAT  >  NC_000913/1158482‑1158767
                                                                                                                                                |                                                                                                                                             
tGGTACCGTTTGGTCTGCACCACATCTGGAACGTCCCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATAGGGCGGGAGACCCGACTGCGGGTAAACTGGCTGGTGACTTc                                                                                                                                           >  4:236209/1‑149 (MQ=255)
tGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTc                                                                                                                                           >  4:216706/1‑149 (MQ=255)
  gTACCGTTTGGTCTTCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGGTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTc                                                                                                                                           <  6:27482/147‑1 (MQ=255)
  gTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTc                                                                                                                                           <  7:372879/147‑1 (MQ=255)
  gTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCt                                                                                                                                         <  6:357506/149‑1 (MQ=255)
  gTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCt                                                                                                                                         >  5:279632/1‑149 (MQ=255)
     ccGTTTGTTCTGCACCAGATCAGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGGAGCAGGTCATGTTTTCGACGTCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGt                                                                                                                                       <  5:98403/148‑1 (MQ=255)
     ccGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTTAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCTTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGt                                                                                                                                       <  2:302551/148‑1 (MQ=255)
           ggTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCaaaa                                                                                                                                 <  7:290946/148‑1 (MQ=255)
               tGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTAc                                                                                                                            >  1:124924/1‑149 (MQ=255)
               tGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTAc                                                                                                                            >  7:60806/1‑149 (MQ=255)
                 caccacATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTATTCAAAATGTAc                                                                                                                            <  5:85562/147‑1 (MQ=255)
                  accacATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGCCTTCCTGTTCAAAATGTACGGt                                                                                                                         >  3:116776/1‑149 (MQ=255)
                  accacATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGGGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGt                                                                                                                         <  5:97903/149‑1 (MQ=255)
                   ccacATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGTGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTc                                                                                                                        >  1:18736/1‑149 (MQ=255)
                    cacaTCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCt                                                                                                                       >  5:126558/1‑149 (MQ=255)
                       aTCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGc                                                                                                                     >  6:162551/1‑148 (MQ=255)
                         cTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAg                                                                                                                  >  6:98504/1‑149 (MQ=255)
                           ggAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCt                                                                                                                >  7:108467/1‑149 (MQ=255)
                           ggAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACGTCCTGTTCAAAATGTACGGTCTGCCAGCt                                                                                                                >  8:99708/1‑149 (MQ=255)
                            gAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCa                                                                                                                   <  6:279632/145‑1 (MQ=255)
                            gAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCa                                                                                                                   <  2:2304/145‑1 (MQ=255)
                                  aCCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCaa                                                                                                         >  2:31854/1‑149 (MQ=255)
                                  aCCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTAAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAATATGTACGGTCTGCCAGCTGCCGCaa                                                                                                         >  7:182341/1‑149 (MQ=255)
                                   ccTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCaa                                                                                                         <  2:18736/148‑1 (MQ=255)
                                       tCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGc                                                                                                     <  1:168327/148‑1 (MQ=255)
                                         cAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTa                                                                                                   <  2:195729/148‑1 (MQ=255)
                                          aGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATc                                                                                                 <  2:124924/149‑1 (MQ=255)
                                          aGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATc                                                                                                 >  7:122941/1‑149 (MQ=255)
                                              gCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTAc                                                                                                                            <  2:309340/118‑1 (MQ=255)
                                                  aTTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCActc                                                                                         <  8:60806/149‑1 (MQ=255)
                                                   ttGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCActct                                                                                        <  1:31854/149‑1 (MQ=255)
                                                     ggttactACACCATCGCAGCAGGTCAGGTTATCCACGGCGACATTCCGCGTTATATGGCGGGTGAGCGGACTGCGGGTAAACTGGCTGGGGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTTCCGCAATTGCTATCTGGCACTCTg                                                                                       <  4:116776/142‑1 (MQ=255)
                                                     ggTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTg                                                                                       <  4:328431/148‑1 (MQ=255)
                                                      gTGACTACACCAACGCAGCAGGTCAGGGTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAATCTGGCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGc                                                                                      <  3:236209/148‑1 (MQ=255)
                                                        gAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTa                                                                                    >  2:393340/1‑148 (MQ=255)
                                                        gAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTa                                                                                    >  5:150254/1‑148 (MQ=255)
                                                           tACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAAcc                                                                                <  1:197429/149‑1 (MQ=255)
                                                                caccGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAATCTGTCTGGTGACTTCCTGTTCAAAATGTCCGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGaa                                                                            >  3:246614/4‑148 (MQ=255)
                                                                   cGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAAcc                                                                        <  5:98504/149‑1 (MQ=255)
                                                                            tcaggtTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCGATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAg                                                               >  2:130571/1‑149 (MQ=255)
                                                                            tcaggtTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGATATCTGGCActct                                                                                        >  7:433553/1‑124 (MQ=255)
                                                                            tcaggtGTTCCACGGCGACATTCCGCTTTATATGGCGGGTGAACCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCActct                                                                                        <  8:433553/124‑1 (MQ=255)
                                                                                       aCGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGtat                                                     >  4:193148/1‑148 (MQ=255)
                                                                                          gCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATg                                                 <  8:122941/149‑1 (MQ=255)
                                                                                           cGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTgg                                                            <  4:397589/137‑1 (MQ=255)
                                                                                           cGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTgg                                                            >  3:397589/1‑137 (MQ=255)
                                                                                            gACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGTTAAACCAGAAAACCGCGCGAAAGTGGGCGGtatt                                                    >  5:116767/1‑144 (MQ=255)
                                                                                            gACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGTTAAACCAGAAAACCGCGCGAAAGTGGGCGGtatt                                                    <  6:116767/144‑1 (MQ=255)
                                                                                                   cgcgTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCcgcg                                        >  2:234328/1‑149 (MQ=255)
                                                                                                            tGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGAc                                <  3:193148/148‑1 (MQ=255)
                                                                                                                 ggTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGtt                          >  8:8930/1‑149 (MQ=255)
                                                                                                                  gTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGGCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTc                         <  3:216706/149‑1 (MQ=255)
                                                                                                                      cccGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGa                     >  3:143622/1‑149 (MQ=255)
                                                                                                                       ccGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGAc                    >  7:169771/1‑149 (MQ=255)
                                                                                                                       ccGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGAc                    >  3:337326/1‑149 (MQ=255)
                                                                                                                         gACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCTAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGAGCTCCGCGGCGCTGACCTCGTTCCTGACCg                  <  6:126558/149‑1 (MQ=255)
                                                                                                                              cGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTAt              <  8:108467/148‑1 (MQ=255)
                                                                                                                                ggTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGAc                    <  6:27381/140‑1 (MQ=255)
                                                                                                                                ggTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGAc                    >  5:27381/1‑140 (MQ=255)
                                                                                                                                ggTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCAc           <  8:169771/149‑1 (MQ=255)
                                                                                                                                 gTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCAcc          >  2:135288/1‑149 (MQ=255)
                                                                                                                                 gTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCAcc          >  2:225431/1‑149 (MQ=255)
                                                                                                                                   aaaCTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGt                                                              <  7:420055/95‑1 (MQ=255)
                                                                                                                                   aaaCTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGt                                                              >  8:420055/1‑95 (MQ=255)
                                                                                                                                    aaCTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGa        <  1:393340/148‑1 (MQ=255)
                                                                                                                                    aaCTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGa        <  1:234328/148‑1 (MQ=255)
                                                                                                                                    aaCTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGAg       <  1:135288/149‑1 (MQ=255)
                                                                                                                                    aaCTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGAg       >  7:17110/1‑149 (MQ=255)
                                                                                                                                     aCTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTTCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGAGc      <  5:162551/149‑1 (MQ=255)
                                                                                                                                       tGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGAGcc     >  5:193710/1‑148 (MQ=255)
                                                                                                                                         tCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGAGCCGAt  <  7:8930/149‑1 (MQ=255)
                                                                                                                                           tggtgaCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGa        <  4:182996/141‑1 (MQ=255)
                                                                                                                                           tggtgaCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGa        >  3:182996/1‑141 (MQ=255)
                                                                                                                                                |                                                                                                                                             
TGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGAGCCGAT  >  NC_000913/1158482‑1158767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: