Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,196,247 A→G 9.0% L375L (TTA→TTG) ‡ icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,196,2470AG 9.0% 254.4 / 10.5 88L375L (TTA→TTG) ‡icdisocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
Reads supporting (aligned to +/- strand):  ref base A (39/41);  new base G (4/4);  total (43/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTAT  >  NC_000913/1196102‑1196395
                                                                                                                                                 |                                                                                                                                                    
aCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAAtt                                                                                                                                                   <  2:400730/149‑1 (MQ=255)
aCATCGGTGACGAATGCCCACTGTTAGAAGCCAAACACGGAACTTCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTCGCTCTATTAATCACTCAGCAGAGATGATGCTGCGCCACATGGGTTGGACCGAACCGGCTGACTTAAtt                                                                                                                                                   >  4:391590/1‑149 (MQ=255)
 cATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGGCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTg                                                                                                                                                  <  4:117513/149‑1 (MQ=255)
      gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTaaa                                                                                                                                             <  5:193366/149‑1 (MQ=255)
       tGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCCGGACAAAGTAAATCCTGGCGCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg                                                                                                                                            <  5:340659/149‑1 (MQ=38)
       tGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAg                                                                                                                                            <  4:151684/149‑1 (MQ=255)
          cGAATGCGCCCTGTTTGAAGCCACCCTCGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCCGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGGTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTa                                                                                                                                         <  5:255641/149‑1 (MQ=255)
           gAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAACTCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTAt                                                                                                                                        <  3:217300/149‑1 (MQ=255)
           gAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTAt                                                                                                                                        <  6:97154/149‑1 (MQ=255)
           gAATGCGCCCTGTTGGAAGCCACCCACTGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGGTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTAt                                                                                                                                        <  3:222785/149‑1 (MQ=255)
            aaTGCGCACTGTTTGAAGCCACCAACGGTACTGCGACGAAATATGCCGGACAGGACAAACTAAATCCTGGATCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATCGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATg                                                                                                                                       >  8:365224/1‑149 (MQ=255)
             aTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATgg                                                                                                                                      <  3:168815/149‑1 (MQ=255)
              tGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGa                                                                                                                                     <  6:102006/149‑1 (MQ=255)
                cgcCCGGTTTGAATCCACACACGTTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGATATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAg                                                                                                                                   <  2:112677/149‑1 (MQ=38)
                 gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAgg                                                                                                                                  >  3:3355/1‑149 (MQ=255)
                        ttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTg                                                                                                                                                  <  1:37571/126‑1 (MQ=255)
                        ttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTg                                                                                                                                                  >  2:37571/1‑126 (MQ=255)
                          gAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCaa                                                                                                                         >  3:259464/1‑149 (MQ=255)
                           aaGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAc                                                                                                                        >  8:382786/1‑149 (MQ=255)
                                acccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcca                                                                                                                    >  8:166549/1‑146 (MQ=25)
                                acccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAg                                                                                                                                            <  2:2580/124‑1 (MQ=255)
                                acccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAg                                                                                                                                            >  1:2580/1‑124 (MQ=255)
                                  ccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCACCGCGaaaa                                                                                                                 >  4:397197/1‑149 (MQ=255)
                                  ccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGaaaa                                                                                                                 >  3:252807/1‑149 (MQ=255)
                                           tGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAAcc                                                                                                         >  6:306938/1‑148 (MQ=255)
                                             cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAt                                                                                                      <  7:382786/149‑1 (MQ=255)
                                               ccGAAATATGCCGGGCAGGACCTAGTAAATCGTGGATCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAATCGGCTGACTTAATTGTTAAAGGTATGGAAGTCGCAATCAACGCGAAAACCGTAACCTATGa                                                                                                    <  1:270307/149‑1 (MQ=255)
                                                cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAg                                                                                                                                            >  2:55357/1‑108 (MQ=255)
                                                cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAg                                                                                                                                            <  1:55357/108‑1 (MQ=255)
                                                cGAAATATGCCGGACAGGACAAAGTAAATCCTGGCTCTACTATTCACTCCGCAGAGATGATGCTGAGCCACAAGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGTTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATTAc                                                                                                   >  2:297264/1‑149 (MQ=255)
                                                    atatGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCg                                                                                               <  4:252807/149‑1 (MQ=255)
                                                      atGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAg                                                                                             >  8:291202/1‑149 (MQ=255)
                                                       tGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGc                                                                                            >  5:64825/1‑149 (MQ=255)
                                                          cGGTCAGTACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGCTTGGACCGAAGCGGCTGACTTAATTGTTAAAGTTATGGAAGTCGCAATCAACGCGAAAACCGTAACCTATGCCATCGAGCGTc                                                                                         >  2:155627/1‑149 (MQ=255)
                                                            gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTg                                                                                       <  4:131111/149‑1 (MQ=255)
                                                               aGGTCAAAGTAAATCCTGGCTCTATTATTCTCTCCGATGATATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgat                                                                                     <  3:10870/148‑1 (MQ=255)
                                                                ggACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatg                                                                                    >  2:201947/1‑148 (MQ=255)
                                                                 gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACCTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAgg                                                                                                                                           <  5:230736/92‑1 (MQ=255)
                                                                 gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAgg                                                                                                                                           >  6:230736/1‑92 (MQ=255)
                                                                 gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTc                                                                                         >  3:284957/1‑142 (MQ=255)
                                                                 gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTc                                                                                         <  2:70597/142‑1 (MQ=255)
                                                                 gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTc                                                                                         >  1:70597/1‑142 (MQ=255)
                                                                 gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTc                                                                                         <  4:284957/142‑1 (MQ=255)
                                                                 gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatgga                                                                                  >  2:86822/1‑149 (MQ=255)
                                                                 gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatgga                                                                                  >  2:55132/1‑149 (MQ=255)
                                                                   cAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAACTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAAc                                                                                                                <  5:302027/117‑1 (MQ=255)
                                                                   cAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAACTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAAc                                                                                                                >  6:302027/1‑117 (MQ=255)
                                                                       gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTa                                                                                                                                         >  1:126513/1‑88 (MQ=18)
                                                                       gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTa                                                                                                                                         <  2:126513/88‑1 (MQ=18)
                                                                       gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt                                                                                                 >  1:308902/1‑128 (MQ=14)
                                                                       gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt                                                                                                 <  2:314596/128‑1 (MQ=14)
                                                                       gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt                                                                                                 <  2:308902/128‑1 (MQ=14)
                                                                       gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt                                                                                                 >  1:314596/1‑128 (MQ=14)
                                                                              cTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgat                                                                                     <  5:405327/133‑1 (MQ=255)
                                                                              cTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgat                                                                                     >  6:405327/1‑133 (MQ=255)
                                                                              cTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActg                                                                     <  7:365224/149‑1 (MQ=255)
                                                                               tGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActgc                                                                    >  1:419305/1‑149 (MQ=255)
                                                                                 gCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActgctg                                                                  <  4:180760/149‑1 (MQ=255)
                                                                                   tctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa                                                                 >  7:281181/1‑148 (MQ=255)
                                                                                   tctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa                                                                 >  8:165499/1‑148 (MQ=255)
                                                                                       ttattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGt                                                            <  7:291202/149‑1 (MQ=255)
                                                                                       ttattCTCACCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGACGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGAGAAAACCGTAACCTATGACTTCGAGCGTCAGATGGATGGCGCTAACCTGCTGAAATg                                                             >  6:101126/1‑148 (MQ=21)
                                                                                          ttCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGtt                                                           >  8:409544/1‑147 (MQ=255)
                                                                                            ctctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCa                                                         >  2:271225/1‑147 (MQ=255)
                                                                                             tctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCagag                                                      >  1:160471/1‑149 (MQ=255)
                                                                                               tcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCTAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCaga                                                       <  7:175537/146‑1 (MQ=255)
                                                                                               tcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCaga                                                       <  7:409544/146‑1 (MQ=255)
                                                                                               tcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGtt                                                    >  5:43754/1‑149 (MQ=255)
                                                                                                 cGCTGAGATGATGCTGCGCCACATTGGTTTGACCGAAGCGTCTGACTTAATTGTTAAAGGTATTGAAGGCGCAATCAACGAGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTg                                                  <  6:64825/149‑1 (MQ=18)
                                                                                                      agaTGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGAGTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAc                                             >  7:375093/1‑149 (MQ=255)
                                                                                                      agaTGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTg                                               <  4:3355/147‑1 (MQ=255)
                                                                                                      agaTGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAc                                             >  8:209202/1‑149 (MQ=255)
                                                                                                         tgatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgc                                           >  1:28048/1‑148 (MQ=255)
                                                                                                          gatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCaga                                                       <  7:116010/135‑1 (MQ=255)
                                                                                                          gatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCaga                                                       >  8:116010/1‑135 (MQ=255)
                                                                                                                 cgcCACATGGGTTGGACCGAATCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatcatc                                    <  8:281181/147‑1 (MQ=255)
                                                                                                                 cgcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatcatc                                    <  6:43754/147‑1 (MQ=255)
                                                                                                                 cgcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatcatc                                    <  4:259464/147‑1 (MQ=255)
                                                                                                                   ccACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa                                <  1:201947/149‑1 (MQ=255)
                                                                                                                    cacaTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa                               >  6:271285/1‑149 (MQ=255)
                                                                                                                            ttGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAAt                       <  2:419305/149‑1 (MQ=255)
                                                                                                                                 ccGAATCTGCTGACTTAATTGTTAATGGTATGGAAGGCGCAATCTACTCGAAAACCGTAACCTATGAATTCGAGCGGCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGt                  <  1:297264/149‑1 (MQ=18)
                                                                                                                                  cGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTa                 <  1:86822/149‑1 (MQ=255)
                                                                                                                                         gcagACTTAATTCTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAAGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGCGTTTGGTGACGCGATCATCGAAAACATGAAAAGCCGTAGTTTGt           >  4:323723/4‑148 (MQ=18)
                                                                                                                                         gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGtt          <  5:271285/149‑1 (MQ=255)
                                                                                                                                          cttaCTTAATTGTTAAAGGTATGGAAGGCGCAATAAACGCGTAATACTTAACCTATGACTTCGTGCTTCTGATGGATGGCGCTATACTGCTGAAATGTTCAGAGTTTTGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGtt          <  3:323723/145‑1 (MQ=18)
                                                                                                                                               ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt     >  4:275704/1‑148 (MQ=255)
                                                                                                                                               ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt     >  7:192540/1‑148 (MQ=255)
                                                                                                                                                 aaTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGGAACCGATGACTTCGAGCGGATGATGGATGGCGGTAAACTGCTGAAATGTTCATATTTTGGTGACGAGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATttat  >  6:116483/1‑149 (MQ=21)
                                                                                                                                                 |                                                                                                                                                    
ACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTAT  >  NC_000913/1196102‑1196395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: