Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 168,474 C→T 79.3% Q331* (CAG→TAG)  fhuA → ferrichrome outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913168,4740CT79.3% 217.2 / 52.8 107Q331* (CAG→TAG) fhuAferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (11/11);  new base T (51/33);  total (63/44)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

GGAACCGTTGAGCCGCTGCCGAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTC  >  NC_000913/168333‑168620
                                                                                                                                             |                                                                                                                                                  
ggAACCGTTGAGCCGCTGCCGAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCt                                                                                                                                             >  5:87264/1‑149 (MQ=255)
    ccGTTGAGCCGCTGCCGAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTgcgc                                                                                                                                         <  2:434126/149‑1 (MQ=255)
            ccGCTGCCGAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTCATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGa                                                                                                                                 <  1:29384/149‑1 (MQ=255)
                  ccGAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGaaaacaa                                                                                                                           <  2:426256/149‑1 (MQ=255)
                   cGAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGaaaacaa                                                                                                                           >  1:326517/1‑148 (MQ=255)
                    gAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGaaaacaaaa                                                                                                                         >  5:343876/1‑149 (MQ=255)
                        ggTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTc                                                                                                                     <  6:87264/149‑1 (MQ=255)
                            aGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCa                                                                                                                  >  5:255731/1‑148 (MQ=255)
                               gTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACCCCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCaaaa                                                                                                               >  3:237256/1‑148 (MQ=255)
                                    ccGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGt                                                                                                         >  5:31501/1‑149 (MQ=255)
                                       aCAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGATAAGATGGTCGGCTACAGCGTCGCTCACGAATTTAACGACCCCTTTAATGTTCGTTAGAACATGAGCTTTGCTTAACACAAAACCTCGCAAAACAGAGTGTa                                                                                                      >  8:307247/1‑149 (MQ=255)
                                       aCAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTa                                                                                                      <  3:94614/149‑1 (MQ=255)
                                         aGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATg                                                                                                    >  1:170944/1‑149 (MQ=255)
                                           aCTTTAATGAAGGGGCGAAGCCCCACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACCGCGTTTGTGGt                                                                                                  >  2:171501/1‑149 (MQ=255)
                                             tttAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAACCAAAACCTCGCAAAACAGCGTTTATGGTTa                                                                                                >  8:247787/1‑149 (MQ=255)
                                             tttAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTa                                                                                                >  4:153636/1‑149 (MQ=255)
                                               tAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATGACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACg                                                                                              >  6:394319/1‑149 (MQ=255)
                                               tAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACg                                                                                              >  5:160423/1‑149 (MQ=255)
                                               tAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACg                                                                                              >  1:402835/1‑149 (MQ=255)
                                                aaTGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACg                                                                                              <  6:255731/148‑1 (MQ=255)
                                                aaTGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACg                                                                                              <  1:45531/148‑1 (MQ=255)
                                                   gAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCCTTTATGGTTACGGCGt                                                                                          >  2:250872/1‑149 (MQ=255)
                                                     aGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCt                                                                                        >  2:415596/1‑149 (MQ=255)
                                                       gggCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGc                                                                                      >  3:296438/1‑149 (MQ=255)
                                                         gCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTc                                                                                    >  3:102994/1‑149 (MQ=255)
                                                           gaagaaCAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTcc                                                                                   >  7:79926/1‑148 (MQ=255)
                                                            aagaaCAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGa                                                                                 <  4:296438/149‑1 (MQ=255)
                                                            aagaaCAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGa                                                                                 >  2:240224/1‑149 (MQ=255)
                                                             agaaCAACACCTATTCTCGTAATGAGAAGATGGTCGTAGACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGAt                                                                                <  6:343876/149‑1 (MQ=255)
                                                                  aacaCCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGc                                                                           >  7:93322/1‑149 (MQ=255)
                                                                  aacaCCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGc                                                                           <  3:153636/149‑1 (MQ=255)
                                                                    cacCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGaaaacaaaa                                                                                                                         <  8:137169/101‑1 (MQ=255)
                                                                    cacCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGaaaacaaaa                                                                                                                         >  7:137169/1‑101 (MQ=255)
                                                                    cacCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGc                                                                                                           >  5:108200/1‑115 (MQ=255)
                                                                    cacCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGc                                                                                                           <  6:108200/115‑1 (MQ=255)
                                                                     acCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACgg                                                                                             >  2:207768/1‑128 (MQ=255)
                                                                     acCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACgg                                                                                             <  1:207768/128‑1 (MQ=255)
                                                                      ccTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAAt                                                                       >  5:15623/1‑149 (MQ=255)
                                                                       cTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATg                                                                      >  3:27707/1‑149 (MQ=255)
                                                                       cTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATg                                                                      >  6:20696/1‑149 (MQ=255)
                                                                       cTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATg                                                                      <  6:31501/149‑1 (MQ=255)
                                                                          ttCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCt                                                                    <  2:326517/148‑1 (MQ=255)
                                                                           tctcGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTa                                                                  >  1:84271/1‑149 (MQ=255)
                                                                           tctcGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTa                                                                  >  6:60552/1‑149 (MQ=255)
                                                                             tcGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTa                                                                  >  7:304751/1‑147 (MQ=255)
                                                                             tcGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAAAAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCAGGCGAATGATTACa                                                                >  7:232452/1‑149 (MQ=255)
                                                                               gTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTAc                                                                 <  4:370159/146‑1 (MQ=255)
                                                                               gTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTAc                                                                 <  6:15623/146‑1 (MQ=255)
                                                                               gTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACa                                                                <  4:27707/147‑1 (MQ=255)
                                                                                tAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGc                                                              >  3:44477/1‑148 (MQ=255)
                                                                                  aTGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCaaa                                                           <  5:60552/149‑1 (MQ=255)
                                                                                     agaagaTGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAg                                                        >  3:373749/1‑149 (MQ=255)
                                                                                     agaagaTGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACa                                                         <  1:61466/148‑1 (MQ=255)
                                                                                        agaTGGTCGGCTACATCTTCGATCACGAATATAACGAGACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAgtgt                                                     <  8:232452/149‑1 (MQ=255)
                                                                                              tCGGCTACAGCTTCGTTCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTCCGGCGTCTGCTCCGAACCGGCGAATGCTTACAGCAAACAGTGTGCGGCa                                               >  8:417775/1‑149 (MQ=255)
                                                                                              tCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCa                                               <  2:402835/149‑1 (MQ=255)
                                                                                              tCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTGAGAACCTGCGCTTTGCTGAAAACAAACCCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATGCGGCGAATGCTTACCGCAAACAGTGTGCGGCa                                               >  8:411954/1‑149 (MQ=255)
                                                                                                ggCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCAt                                              <  8:304751/148‑1 (MQ=255)
                                                                                                ggCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGAACACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCAtt                                             <  4:102994/149‑1 (MQ=255)
                                                                                                   tACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAgc                                          >  3:25712/1‑149 (MQ=255)
                                                                                                    aCAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAgcg                                         >  8:294470/1‑149 (MQ=255)
                                                                                                        cTTCTATCACGAATTTACCGACACCTTTACTGTGCGTTAGAACCTTCGCTTTGCTGAAAACAAAACCACGCAAAACAGCGTGTATGTTTTCGGCGCATGCTCCGATCCGGCGAATTCTTACAGCAAACAGTGTGCGGCATGAGCGCCAg                                     <  7:307247/149‑1 (MQ=255)
                                                                                                         ttCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGc                                    >  2:194903/1‑149 (MQ=255)
                                                                                                             aTCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGa                                 >  3:257315/1‑148 (MQ=255)
                                                                                                              tCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGt                                                                                          >  4:304244/1‑90 (MQ=255)
                                                                                                              tCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGt                                                                                          <  3:304244/90‑1 (MQ=255)
                                                                                                               cACGAATTTAACGACACCTTTAATGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAACCCTCGCAAAACCGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCAt                                              >  2:215713/1‑133 (MQ=255)
                                                                                                                aCGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCg                                   >  1:39916/1‑143 (MQ=255)
                                                                                                                aCGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCg                                   <  2:39916/143‑1 (MQ=255)
                                                                                                                aCGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCAGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATa                               >  4:191517/1‑147 (MQ=255)
                                                                                                                aCGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACa                                                         >  4:110797/1‑121 (MQ=255)
                                                                                                                aCGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACa                                                         <  3:110797/121‑1 (MQ=255)
                                                                                                                  gAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAgg                           <  1:240224/149‑1 (MQ=255)
                                                                                                                  gAAATTAACGCCACCTTTACTGTGCTTTAGAACCTGCGCTTTGCTGACAACAAAACCTCGAAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGTCGAATGCTTACAGCAAACAGTGTGAGGCATTAGCGCCAGCGGATaaatg                           >  5:70143/1‑147 (MQ=255)
                                                                                                                   aaTTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGc                          <  4:257315/149‑1 (MQ=255)
                                                                                                                    aTTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAACCCACGCAAAACAGCGTTTATGGTTAAGGCGTCTGCTCCGATCCGGCGAATGCTTACAGAAAACAGTGTGCGGCATTAGCGCGAGCGGATAAAGGc                          >  6:97533/1‑148 (MQ=255)
                                                                                                                     tttAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCa                        <  1:194903/149‑1 (MQ=255)
                                                                                                                     tttAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCa                        <  5:394319/149‑1 (MQ=255)
                                                                                                                      ttAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAACGGCCAt                       >  1:84829/1‑149 (MQ=255)
                                                                                                                       tAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCAt                       >  1:335111/1‑148 (MQ=255)
                                                                                                                          cGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATgg                                                                                                   <  3:1227/69‑1 (MQ=255)
                                                                                                                          cGACACCTTTACTGAGACTTAGAACCTGCGCTTTTCTGAAAACAAAACCTCGCAAAACAGCGATAATgg                                                                                                   >  4:1227/1‑69 (MQ=255)
                                                                                                                           gACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCAGAAAACAAAACCTCGCAACACAGCGTGTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCt                  <  6:70143/149‑1 (MQ=255)
                                                                                                                             cacCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTgg                <  3:247357/149‑1 (MQ=255)
                                                                                                                              acCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTgg                >  3:196783/1‑148 (MQ=255)
                                                                                                                              acCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGAGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTTTGCg                                                  >  1:219418/1‑114 (MQ=255)
                                                                                                                              acCTTTACTGTGAGTTAGAACCTGAGCTTTGATGAAAACAAAACCTCGCAAAACAGCTTTTATGGTTACGGCGTCTGCTCCGTTCCTGCGAATGCTTACAGCAAACAGTGTTCg                                                  <  2:219418/114‑1 (MQ=255)
                                                                                                                                cTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGc                                                                           >  8:70073/1‑87 (MQ=255)
                                                                                                                                cTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGc                                                                           <  7:70073/87‑1 (MQ=255)
                                                                                                                                cTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAg                                                               <  6:67447/99‑1 (MQ=255)
                                                                                                                                cTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGc               >  8:146788/1‑147 (MQ=255)
                                                                                                                                cTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGc               <  7:146788/147‑1 (MQ=255)
                                                                                                                                cTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCAc             >  8:276631/1‑149 (MQ=255)
                                                                                                                                 tttACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACg            >  3:163507/1‑149 (MQ=255)
                                                                                                                                   tACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCa                        <  2:210496/135‑1 (MQ=255)
                                                                                                                                   tACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCa                        >  1:210496/1‑135 (MQ=255)
                                                                                                                                      tgtgCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTaaa        >  7:267191/1‑148 (MQ=255)
                                                                                                                                      tgtgCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTg                 <  3:261499/139‑1 (MQ=255)
                                                                                                                                      tgtgCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTg                 >  4:261499/1‑139 (MQ=255)
                                                                                                                                           gTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATAcgtc  >  4:101569/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
GGAACCGTTGAGCCGCTGCCGAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGTC  >  NC_000913/168333‑168620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: