Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,819,040 C→G 5.3% L136F (TTG→TTC chbR ← repressor of chb operon for N,N'‑diacetylchitobiose utilization

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,819,0400CG 5.3% 287.2 / 2.5 87L136F (TTG→TTCchbRrepressor of chb operon for N,N'‑diacetylchitobiose utilization
Reads supporting (aligned to +/- strand):  ref base C (40/40);  new base G (2/3);  total (44/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGTGGCAC  >  NC_000913/1818893‑1819188
                                                                                                                                                   |                                                                                                                                                    
ctctTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCTGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCCACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCa                                                                                                                                                     <  4:87803/149‑1 (MQ=255)
  ctTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAAt                                                                                                                                                   >  1:152990/1‑149 (MQ=255)
   tttATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTGAAt                                                                                                                                                   >  8:144513/1‑148 (MQ=255)
   tttATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTGAAt                                                                                                                                                   >  8:138226/1‑148 (MQ=255)
   tttATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCaa                                                                                                                                                    <  5:180121/147‑1 (MQ=255)
   tttATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAAt                                                                                                                                                   >  2:302346/1‑148 (MQ=255)
   tttATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAAt                                                                                                                                                   >  6:184680/1‑148 (MQ=255)
   tttATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAAt                                                                                                                                                   >  2:189457/1‑148 (MQ=255)
          tGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAAt                                                                                                                                           <  1:65515/149‑1 (MQ=255)
           gCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAAt                                                                                                                                           >  3:360729/1‑148 (MQ=255)
           gCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAAt                                                                                                                                           >  8:14442/1‑148 (MQ=255)
             aTCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCAc                                                                                                                                        >  3:51893/1‑149 (MQ=255)
               cTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATAACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAAACATCTCAACAACCTCTTCCAGCCCTGTTTCGCGGAAATTAAATGAAGAAATCCCTg                                                                                                                                      >  6:213966/1‑149 (MQ=255)
                  ttCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTGAATGAAGAAATCACt                                                                                                                                       <  7:144513/145‑1 (MQ=255)
                  ttCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTGAATGAAGAAATCACt                                                                                                                                       <  7:138226/145‑1 (MQ=255)
                   tCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGttt                                                                                                                                   <  2:152990/148‑1 (MQ=255)
                   tCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGttt                                                                                                                                   <  7:37786/148‑1 (MQ=255)
                    cTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTc                                                                                                                                  <  2:277971/148‑1 (MQ=255)
                       ccGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTCAACGGTTAATGACATAAAAAGTCACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCcac                                                                                                                               <  2:7534/148‑1 (MQ=255)
                       ccGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTcc                                                                                                                                 <  7:121748/146‑1 (MQ=255)
                          tACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATgaa                                                                                                                                                <  7:204198/128‑1 (MQ=255)
                          tACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATgaa                                                                                                                                                >  8:204198/1‑128 (MQ=255)
                             ttttCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGt                                                                                                                         >  7:159355/1‑148 (MQ=255)
                             ttttCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACAATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAATTCACTGTTTCCACATAggg                                                                                                                         >  6:360751/1‑147 (MQ=255)
                                  aGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGaaa                                                                                                                   <  7:14442/149‑1 (MQ=255)
                                    ccACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAg                                                                                                                 >  4:311609/1‑149 (MQ=255)
                                      aCTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGaa                                                                                                                    >  7:4379/1‑144 (MQ=255)
                                      aCTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGaa                                                                                                                    <  8:4379/144‑1 (MQ=255)
                                      aCTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCg                                                                                                               <  5:273345/149‑1 (MQ=255)
                                      aCTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCg                                                                                                               >  8:258444/1‑149 (MQ=255)
                                          cGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTa                                                                                                            <  1:207927/148‑1 (MQ=255)
                                          cGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTAt                                                                                                           <  4:51893/149‑1 (MQ=255)
                                          cGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTAt                                                                                                           <  1:189457/149‑1 (MQ=255)
                                                  catcaATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTg                                                                                                                                      <  4:395721/114‑1 (MQ=255)
                                                  catcaATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTg                                                                                                                                      >  3:395721/1‑114 (MQ=255)
                                                       atccCCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGGTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATAc                                                                                              <  4:315445/149‑1 (MQ=255)
                                                       atcaCCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATAc                                                                                              <  5:184680/149‑1 (MQ=255)
                                                       atcaCCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATAc                                                                                              <  8:159355/149‑1 (MQ=255)
                                                        tcaCCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTc                                                                                                                                  <  4:155815/112‑1 (MQ=255)
                                                        tcaCCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTc                                                                                                                                  >  3:155815/1‑112 (MQ=255)
                                                        tcaCCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATAcc                                                                                             >  1:73265/1‑149 (MQ=255)
                                                           ccTGTTATTCGCGGTAATGCCGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGACCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCTGTATACCTGc                                                                                          <  5:213966/149‑1 (MQ=255)
                                                            cTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGc                                                                                          >  5:395034/1‑148 (MQ=255)
                                                                 cttcGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGc                                                                                     <  1:31161/148‑1 (MQ=255)
                                                                   tcGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGa                                                                                   >  8:8206/1‑148 (MQ=255)
                                                                    cgcgGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGAc                                                                                  <  7:442570/148‑1 (MQ=255)
                                                                       ggTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGCGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAg                                                                              <  5:116106/149‑1 (MQ=255)
                                                                         tAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGtata                                                                                               >  6:31220/1‑130 (MQ=255)
                                                                         tAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGtata                                                                                               <  5:31220/130‑1 (MQ=255)
                                                                           aTGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAAt                                                                          <  2:49903/149‑1 (MQ=255)
                                                                           aTGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAAt                                                                          <  7:8206/149‑1 (MQ=255)
                                                                            tGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCcaca                                                                                                                              <  2:268138/96‑1 (MQ=255)
                                                                            tGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCcaca                                                                                                                              >  1:268138/1‑96 (MQ=255)
                                                                              aCGTAAACGGTTAATGACATAAAAAGTCCCCATCTCAACAAACTATTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACAGAGGTGAGAACAGCGTTATTGGTCCGTTATACCTCCGAAGAGACAAAGCACTAAg                                                                       >  7:314736/1‑149 (MQ=255)
                                                                              aCGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATaa                                                                        <  4:360729/148‑1 (MQ=255)
                                                                              aCGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAg                                                                       <  1:302346/149‑1 (MQ=255)
                                                                                 tAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGc                                                                     >  1:75427/1‑148 (MQ=255)
                                                                                     cGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGcaacaa                                                                >  3:231744/1‑149 (MQ=255)
                                                                                            tGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAgg                                                         >  1:427668/1‑149 (MQ=255)
                                                                                              aCATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTg                                                                                                         >  4:69574/1‑99 (MQ=255)
                                                                                              aCATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGc                                                                                     >  1:223752/1‑119 (MQ=255)
                                                                                              aCATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGc                                                                                     <  2:223752/119‑1 (MQ=255)
                                                                                                aTAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAAt                                                     >  4:298121/1‑149 (MQ=255)
                                                                                                 tAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAAtg                                                    >  2:138392/1‑149 (MQ=255)
                                                                                                  aaaaaGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATCAAGAAAACACTGTATCCCCATAGCAGACAAAAGCGTTAAAGGTCCTGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAAAGGCAGGTAAtg                                                    >  3:124242/1‑148 (MQ=255)
                                                                                                      aGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATgctgct                                               <  2:75427/149‑1 (MQ=255)
                                                                                                          aCCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCaa                                            >  8:21191/1‑148 (MQ=255)
                                                                                                              tctcAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACaaa                                                                               >  1:341321/1‑109 (MQ=255)
                                                                                                              tctcAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACaaa                                                                               <  2:341321/109‑1 (MQ=255)
                                                                                                                  aacaaACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGtt                                   >  6:35833/1‑149 (MQ=255)
                                                                                                                  aacaaACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGt                                    <  2:73265/148‑1 (MQ=255)
                                                                                                                      aaCTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACt                               >  7:49607/1‑149 (MQ=255)
                                                                                                                           ttCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGATTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGAt                            <  5:360751/147‑1 (MQ=255)
                                                                                                                              cAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCaa                       >  3:149510/1‑149 (MQ=255)
                                                                                                                              cAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCaa                       >  4:128002/1‑149 (MQ=255)
                                                                                                                                gCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACg                     >  8:172789/1‑149 (MQ=255)
                                                                                                                                 cccTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGt                    >  5:78531/1‑149 (MQ=255)
                                                                                                                                 caatGTTTCTCGGAAATTAAATAAAGAAATCACTCTAACCACATAGGTCAGAAAAGCGTAAATGGTCAGGTAAACCTGCGAAGCGACAAAGCAATAACGCAACAATGGAAGGTAATGATGCTCAAAAAAGCGTTTACTGATCCCAACGt                    >  4:100294/4‑149 (MQ=255)
                                                                                                                                  ccTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGtt                   <  3:311609/149‑1 (MQ=255)
                                                                                                                                   gtGTTTAGCGGAAATTGAATGAAGAAATCTCTGTTTCCACTTAGGTGAGAAAAGCGTTATTGGTCCGTTTTACCTGCGAAGCGACAAAGCTATAAGGCTACAATGGCAGGTATTTCTGCTCAAAAAAGCGTTTACTGATCCCAACGttg                  <  3:100294/148‑2 (MQ=255)
                                                                                                                                         cgcgGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAatat             <  4:231744/148‑1 (MQ=255)
                                                                                                                                          gcgGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATgc           <  4:124242/149‑1 (MQ=255)
                                                                                                                                            ggAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATgcgc         >  7:185665/1‑149 (MQ=255)
                                                                                                                                            ggAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATgc           <  4:72688/147‑1 (MQ=255)
                                                                                                                                            ggAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATgc           >  3:72688/1‑147 (MQ=255)
                                                                                                                                              aaaTTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGt       <  7:21191/149‑1 (MQ=255)
                                                                                                                                                aTTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGTgg     >  1:226065/1‑149 (MQ=255)
                                                                                                                                                   cAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGTGGCAc  >  8:231709/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTCTTTATCATGCATCTTTTCTACCGTACTTTTCAGCCACTGCGGTACATCATCAATCACCTGTTCTTCGCGGTAATGACGTAAACGGTTAATGACATAAAAAGTAACCATCTCAACAAACTCTTCCAGCCCTGTTTCGCGGAAATTCAATGAAGAAATCACTGTTTCCACATAGGTGAGAAAAGCGTTATTGGTCCGGTATACCTGCGAAGCGACAAAGCAATAAGGCAACAATGGCAGGTAATGCTGCTCAAAAAAGCGTTTACTGATCCCAACGTTCAATATGCGCGTGGCAC  >  NC_000913/1818893‑1819188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: