Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,824,688 G→T 5.3% P84T (CCT→ACT)  ves ← cold‑ and stress‑inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,824,6880GT 5.3% 255.2 / 2.2 75P84T (CCT→ACT) vescold‑ and stress‑inducible protein
Reads supporting (aligned to +/- strand):  ref base G (37/34);  new base T (2/2);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

CGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTA  >  NC_000913/1824543‑1824835
                                                                                                                                                 |                                                                                                                                                   
cgTTAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTg                                                                                                                                                  <  2:81873/149‑1 (MQ=255)
        aaaGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGtt                                                                                                                                          >  4:64706/1‑149 (MQ=255)
        aaaGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGtt                                                                                                                                          <  1:384779/149‑1 (MQ=255)
            gtagtaAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTaa                                                                                                                                      <  8:27597/149‑1 (MQ=255)
            gtagtaAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTaa                                                                                                                                      <  8:34431/149‑1 (MQ=255)
             tagtaAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAg                                                                                                                                     <  2:49737/149‑1 (MQ=255)
               gtaAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAgg                                                                                                                                    >  5:181886/1‑148 (MQ=255)
                taAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTa                                                                                                                                  >  5:68144/1‑149 (MQ=255)
                         gtTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAg                                                                                                                         >  7:78119/1‑149 (MQ=255)
                          ttCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATCCTGTTGAAATCCATCTACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGc                                                                                                                        <  4:317267/149‑1 (MQ=255)
                              gCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTc                                                                                                                    >  8:135224/1‑149 (MQ=255)
                                aaTTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGCCATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTg                                                                                                                  >  2:90225/1‑149 (MQ=255)
                                aaTTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTg                                                                                                                  >  2:130846/1‑149 (MQ=255)
                                 aTTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTgc                                                                                                                 <  1:415313/149‑1 (MQ=255)
                                     tGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCAACTGGTCCGCTGCAAAGGCAAAATGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCtt                                                                                                             >  2:318189/1‑149 (MQ=255)
                                           tCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAgg                                                                                                       >  1:160429/1‑149 (MQ=255)
                                            cGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAgg                                                                                                       <  7:295867/148‑1 (MQ=255)
                                                ttACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTaaa                                                                                                                          >  7:364130/1‑125 (MQ=255)
                                                ttACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTaaa                                                                                                                          <  8:364130/125‑1 (MQ=255)
                                                ttACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCAt                                                                                                  >  4:199429/1‑149 (MQ=255)
                                                   cAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCAtctc                                                                                               <  4:302162/149‑1 (MQ=255)
                                                    aGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAgg                                                                                                                                    >  6:373864/1‑111 (MQ=255)
                                                    aGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAgg                                                                                                                                    <  5:373864/111‑1 (MQ=255)
                                                    aGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGTGCAtc                                                                                                 <  2:228210/146‑1 (MQ=255)
                                                    aGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCAtc                                                                                                 >  1:228210/1‑146 (MQ=255)
                                                    aGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTcg                                                                                              >  1:235965/1‑149 (MQ=255)
                                                      aCATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATg                                                                                                                                >  5:69014/1‑113 (MQ=255)
                                                      aCATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATg                                                                                                                                <  6:69014/113‑1 (MQ=255)
                                                        aTCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTcgccgc                                                                                          <  1:130846/149‑1 (MQ=255)
                                                        aTCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTcgccgc                                                                                          >  6:98862/1‑149 (MQ=255)
                                                        aTCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTcgcc                                                                                            >  6:264773/1‑147 (MQ=255)
                                                        aTCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTcgcc                                                                                            <  5:264773/147‑1 (MQ=255)
                                                         tCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCgccgcc                                                                                         >  7:223538/1‑149 (MQ=255)
                                                         tCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCgccgcc                                                                                         >  3:316094/1‑149 (MQ=255)
                                                            aGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGTCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTc                                                                                      >  5:131014/1‑149 (MQ=255)
                                                             ggCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTcc                                                                                     >  4:211615/1‑149 (MQ=255)
                                                             ggCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTcc                                                                                     <  7:135224/149‑1 (MQ=255)
                                                              gCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCa                                                                                    <  1:90225/149‑1 (MQ=255)
                                                                gtgtCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCttt                                                                                                            <  7:318515/123‑1 (MQ=255)
                                                                gtgtCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCttt                                                                                                            >  8:318515/1‑123 (MQ=255)
                                                                   tCATGAGGTTGAACTCCATCGACATCTGCCCTGCCGTCAGTGTCGCTTTTACCACCTGGTCCGCTGCGAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAAc                                                                               <  5:18759/149‑1 (MQ=255)
                                                                     atgatgTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTACCAGCAACGt                                                                             >  1:178627/1‑149 (MQ=255)
                                                                        atgTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCa                                                                           <  6:181886/148‑1 (MQ=255)
                                                                             gAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTa                                                                                                                                  >  8:20754/1‑88 (MQ=255)
                                                                             gAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTa                                                                                                                                  <  7:20754/88‑1 (MQ=255)
                                                                                aTCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATTGTTAAAGCGGTc                                                                                                                    <  8:438528/99‑1 (MQ=255)
                                                                                aTCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTc                                                                                                                    >  7:438528/1‑99 (MQ=255)
                                                                                     tCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGGCCCTATCCGTTCCATg                                                             >  6:59815/1‑149 (MQ=255)
                                                                                     tCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGGTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATg                                                             >  3:43275/1‑149 (MQ=255)
                                                                                            ctgccctgccGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAGAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGa                                                       >  4:392040/1‑148 (MQ=255)
                                                                                               ccctgccGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGaaa                                                     <  3:64706/147‑1 (MQ=255)
                                                                                                 ctgccGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACaaa                                                 <  2:235965/149‑1 (MQ=255)
                                                                                                    ccGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAGAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGa                                               <  3:392040/148‑1 (MQ=255)
                                                                                                      gTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTcc                                                                                     >  4:184943/1‑108 (MQ=255)
                                                                                                      gTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTcc                                                                                     <  3:184943/108‑1 (MQ=255)
                                                                                                         aGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTc                                         <  6:68144/149‑1 (MQ=255)
                                                                                                                       caccTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGTAAACAAAGAAAACTCCCCATTCGCCGCga                           >  6:367075/1‑149 (MQ=255)
                                                                                                                       caccTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGAGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTcgcc                               >  6:58608/1‑145 (MQ=255)
                                                                                                                             gTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgatggatg                     <  3:211615/149‑1 (MQ=255)
                                                                                                                             gTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgatggat                      <  5:58608/148‑1 (MQ=255)
                                                                                                                                 gCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAAt                 <  2:160429/149‑1 (MQ=255)
                                                                                                                                  cTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATg                >  7:20535/1‑149 (MQ=255)
                                                                                                                                     cAAAGGCAAAAGTCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTc                                                                                      <  6:99797/76‑1 (MQ=255)
                                                                                                                                     cAAAGGCAAAAGTCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTc                                                                                      >  5:99797/1‑76 (MQ=255)
                                                                                                                                     cAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTg             <  8:78119/149‑1 (MQ=255)
                                                                                                                                     cAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTg             <  3:199429/149‑1 (MQ=255)
                                                                                                                                      aaaGGCAAAAGTCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTgg            <  6:131014/149‑1 (MQ=255)
                                                                                                                                        aGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCa          >  1:251493/1‑149 (MQ=255)
                                                                                                                                          gCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACg        >  7:422025/1‑149 (MQ=255)
                                                                                                                                          gCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACg        >  2:141144/1‑149 (MQ=255)
                                                                                                                                           cAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTCGCACg        >  5:202403/1‑148 (MQ=255)
                                                                                                                                            aaaaGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGcc      <  8:223538/149‑1 (MQ=255)
                                                                                                                                              aaGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTc                                         <  4:191676/112‑1 (MQ=255)
                                                                                                                                              aaGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTc                                         >  3:191676/1‑112 (MQ=255)
                                                                                                                                              aaGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAg    >  5:374196/1‑149 (MQ=255)
                                                                                                                                                 gCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTa  >  4:233480/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTA  >  NC_000913/1824543‑1824835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑