Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,905,629 T→A 5.1% intergenic (+370/‑59) yobD → / → mntP UPF0266 family inner membrane protein/putative Mn(2+) efflux pump, mntR‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,905,6290TA 5.1% 254.8 / 3.8 78intergenic (+370/‑59)yobD/mntPUPF0266 family inner membrane protein/putative Mn(2+) efflux pump, mntR‑regulated
Reads supporting (aligned to +/- strand):  ref base T (39/35);  new base A (2/2);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01

ATTTCCAGATTCCGGAAATGTACGTGTCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATA  >  NC_000913/1905481‑1905775
                                                                                                                                                    |                                                                                                                                                  
aTTTCCAGATTCCGGAAATGTACGTGTCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGt                                                                                                                                                    <  1:49065/149‑1 (MQ=255)
     cAGATTCCGGAAATGTACGTGTCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGc                                                                                                                                                <  5:140983/148‑1 (MQ=255)
     cAGATTCCGGAAATGTACGTGTCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGAGAGACCATATGCACATCAATCGCTATTCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGc                                                                                                                                                <  7:305266/148‑1 (MQ=255)
            cGGAAATGTACGTGTCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGc                                                                                                                                        >  8:228477/1‑149 (MQ=255)
            cGGAAATGTACGTGTCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGAATGCATGTTTGc                                                                                                                                        >  1:253723/1‑149 (MQ=255)
             ggAAATGTACGTGTCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCt                                                                                                                                       >  6:229140/1‑149 (MQ=255)
               aaaTGTACGTGTCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTgg                                                                                                                                     >  5:263907/1‑149 (MQ=255)
                aaTGTACGTGTCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTggg                                                                                                                                    <  5:76988/149‑1 (MQ=255)
                 aTGTACGTGTCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTgggg                                                                                                                                   <  6:10227/149‑1 (MQ=255)
                      cGTGTCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCgat                                                                                                                              <  5:144719/149‑1 (MQ=255)
                       gtgtCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCgat                                                                                                                              >  1:122814/1‑148 (MQ=255)
                        tgtCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCgatga                                                                                                                            <  5:229140/149‑1 (MQ=255)
                          tCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTgg                                                                                                                                     <  1:44685/138‑1 (MQ=255)
                          tCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTgg                                                                                                                                     >  2:44685/1‑138 (MQ=255)
                           cAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGAtgt                                                                                                                         >  1:228532/1‑149 (MQ=255)
                             aCATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATgtgt                                                                                                                       >  3:378875/1‑149 (MQ=255)
                             aCATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGAATGCATGTTTGCTGGGGGCGATGATgtgt                                                                                                                       <  2:253723/149‑1 (MQ=255)
                                  cTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTAt                                                                                                                  <  4:378875/149‑1 (MQ=255)
                                   tCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTgg                                                                                                                                     <  4:63237/129‑1 (MQ=255)
                                   tCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATg                                                                                                                 >  1:42513/1‑149 (MQ=255)
                                    cGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATg                                                                                                                 >  8:164/1‑148 (MQ=255)
                                         cAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATAcc                                                                                                           >  5:181533/1‑149 (MQ=255)
                                             aCGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCgg                                                                                                       >  6:241668/1‑149 (MQ=255)
                                               gTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTc                                                                                                     <  5:241668/149‑1 (MQ=255)
                                                   cACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGa                                                                                                 <  7:228477/149‑1 (MQ=255)
                                                    aCGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGa                                                                                                 >  3:58393/1‑148 (MQ=255)
                                                       tACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACAtt                                                                                             >  4:301730/1‑149 (MQ=255)
                                                          ggACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGt                                                                                           >  1:306999/1‑148 (MQ=255)
                                                          ggACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTc                                                                                          <  7:164/149‑1 (MQ=255)
                                                           gACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCa                                                                                         <  3:301730/149‑1 (MQ=255)
                                                            aCTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCAt                                                                                        <  6:263907/149‑1 (MQ=255)
                                                            aCTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCAt                                                                                        <  2:122814/149‑1 (MQ=255)
                                                            aCTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCAt                                                                                        >  5:157614/1‑149 (MQ=255)
                                                             cTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCAt                                                                                        >  4:373478/1‑148 (MQ=255)
                                                              tGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAACCCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATg                                                                                       >  5:1151/1‑148 (MQ=255)
                                                                aaTACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGaa                                                                                     >  7:351568/1‑148 (MQ=255)
                                                                aaTACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGaa                                                                                     >  7:38317/1‑148 (MQ=255)
                                                                     tttCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAg                                                                                                   <  8:195309/129‑1 (MQ=255)
                                                                     tttCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAg                                                                                                   >  7:195309/1‑129 (MQ=255)
                                                                     tttCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCAc                                                                               >  5:186499/1‑149 (MQ=255)
                                                                     tttCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTAGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCAc                                                                               >  3:223653/1‑149 (MQ=255)
                                                                         agtcagGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGc                                                                            >  1:279453/1‑148 (MQ=255)
                                                                          gtcagGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTAGGGATACCCCGGTCAGGACATTGTCTTGAATATCACTGCTa                                                                          >  7:403771/1‑149 (MQ=255)
                                                                             agGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTg                                                                       <  8:351568/149‑1 (MQ=255)
                                                                                  agaCCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGttctt                                                                  <  6:157614/149‑1 (MQ=255)
                                                                                       atatGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGc                                                             >  2:10077/1‑149 (MQ=255)
                                                                                        tatGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCgat                                                                                                                              >  4:343088/1‑83 (MQ=255)
                                                                                        tatGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCgat                                                                                                                              <  3:343088/83‑1 (MQ=255)
                                                                                            cacaTCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGttt                                                         <  3:373478/148‑1 (MQ=255)
                                                                                            cacaTCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTg                                                        <  4:235672/149‑1 (MQ=255)
                                                                                            cacaTCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTACATGTAGGCATGTTTGCTGGGGGCGAGGATGTGTTTTATGGATACCACTGTCAGGACATTGGCATGAAGATCACTGCTACGGGTCGTCTAGCGTTTg                                                        >  8:206751/1‑149 (MQ=255)
                                                                                            cacaTCAATCGCTATGCCTCGATGAACGCGATAACCATCACGGCTTGTTGGTCATCTCTGCATGTTTGCTGGCTGCGATGATGTTTTTTCTTGATACATCGGTCAGGACATTGTCATGAATATCACTGCTACTGATCTTCTTGCGTTTg                                                        <  6:188920/149‑1 (MQ=255)
                                                                                             acaTCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTgg                                                       >  7:172427/1‑149 (MQ=255)
                                                                                             acaTCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTgg                                                       >  6:36390/1‑149 (MQ=255)
                                                                                             acaTCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTgg                                                       >  2:252489/1‑149 (MQ=255)
                                                                                               aTCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGc                                                                            >  2:357719/1‑126 (MQ=255)
                                                                                               aTCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGc                                                                            <  1:357719/126‑1 (MQ=255)
                                                                                               aTCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTa                                                     >  7:286744/1‑149 (MQ=255)
                                                                                                tCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGTTAt                                                    <  8:286744/149‑1 (MQ=255)
                                                                                                tCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTAt                                                    >  2:339925/1‑149 (MQ=255)
                                                                                                  aaTCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGt                                                  >  1:395619/1‑149 (MQ=255)
                                                                                                   aTCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTc                                                 <  8:172427/149‑1 (MQ=255)
                                                                                                   aTCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTc                                                 <  2:228532/149‑1 (MQ=255)
                                                                                                       cTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGAATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCgatg                                             >  1:76053/1‑149 (MQ=255)
                                                                                                          tGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCgatgg                                            <  8:38317/147‑1 (MQ=255)
                                                                                                                    aaCGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTtgctg                                <  2:395619/149‑1 (MQ=255)
                                                                                                                     aCGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTtgctgc                               <  1:252489/149‑1 (MQ=255)
                                                                                                                     aCGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTtgctgc                               <  2:306999/149‑1 (MQ=255)
                                                                                                                     aCGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTAGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTtgctgc                               <  4:223653/149‑1 (MQ=255)
                                                                                                                           aaaCCATCACGGCTTGTTGTTCATGAATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAAt                         <  2:76053/149‑1 (MQ=255)
                                                                                                                             aCCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCg                       <  6:1151/149‑1 (MQ=255)
                                                                                                                              ccATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGGCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCgg                      <  5:36390/149‑1 (MQ=255)
                                                                                                                                aTCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTtgc                                  >  4:232102/1‑135 (MQ=255)
                                                                                                                                aTCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTtgc                                  <  3:232102/135‑1 (MQ=255)
                                                                                                                                   aCGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAg                 >  6:243653/1‑149 (MQ=255)
                                                                                                                                     ggCTTGTTGTTCATGTATGCATGTTTGATGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGt               <  7:206751/149‑1 (MQ=255)
                                                                                                                                                 aTGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCa    >  4:184795/1‑148 (MQ=255)
                                                                                                                                                   gTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCCTa  >  7:408634/1‑148 (MQ=255)
                                                                                                                                                   gTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATa  >  2:168000/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATTTCCAGATTCCGGAAATGTACGTGTCAACATACTCGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATA  >  NC_000913/1905481‑1905775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: