Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,113,178 T→C 6.8% intergenic (‑117/+256) rfbB ← / ← wcaN dTDP‑glucose 4,6 dehydratase, NAD(P)‑binding/putative regulatory subunit for GalU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,113,1780TC 6.8% 179.1 / 2.7 61intergenic (‑117/+256)rfbB/wcaNdTDP‑glucose 4,6 dehydratase, NAD(P)‑binding/putative regulatory subunit for GalU
Reads supporting (aligned to +/- strand):  ref base T (23/32);  new base C (2/2);  total (26/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCT  >  NC_000913/2113033‑2113322
                                                                                                                                                 |                                                                                                                                                
ccTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACtgcatgca                                                                                                                                               >  5:153743/1‑149 (MQ=255)
 cTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTgcatgcat                                                                                                                                              <  8:152719/149‑1 (MQ=255)
 cTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTgcatgcat                                                                                                                                              <  4:223953/149‑1 (MQ=255)
     gcCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCt                                                                                                                                          <  8:25971/149‑1 (MQ=255)
      ccaccaGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTa                                                                                                                                         >  2:406659/1‑149 (MQ=255)
      ccaccaGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTa                                                                                                                                         <  6:287459/149‑1 (MQ=255)
      ccaccaGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTa                                                                                                                                         <  5:204723/149‑1 (MQ=255)
       caccaGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTat                                                                                                                                        <  6:36719/149‑1 (MQ=255)
       caccaGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCAAGCATGCCTat                                                                                                                                        >  2:85201/1‑149 (MQ=255)
        accaGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATTCTGTGAAATAAGCGGCTCTCAGTTTGAATAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTatg                                                                                                                                       <  5:274488/149‑1 (MQ=255)
        accaGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTatg                                                                                                                                       <  7:66586/149‑1 (MQ=255)
         ccaGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTatga                                                                                                                                      <  3:192254/149‑1 (MQ=255)
            gTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGAtgt                                                                                                                                   <  8:1508/149‑1 (MQ=255)
               aCAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACtgcatgca                                                                                                                                               <  8:288283/134‑1 (MQ=255)
               aCAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACtgcatgca                                                                                                                                               >  7:288283/1‑134 (MQ=255)
                 aaGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGAc                                                                                                                                >  2:6549/1‑147 (MQ=255)
                 aaGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCACGCATGCCTATGATGTGAc                                                                                                                                >  4:289130/1‑147 (MQ=255)
                 aaGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTATCAGTTTGATTAATAGAGGTATTAATTCACGCTAAATCACATTGCTTTAAATCAACCCGAACAATTCACGCATTCCTATGATGTGACg                                                                                                                               >  6:177432/1‑148 (MQ=255)
                    tATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCg                                                                                                                           <  5:134554/149‑1 (MQ=255)
                         tCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTAc                                                                                                                       <  3:236882/148‑1 (MQ=255)
                               aaTTTTTTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCCCGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCACGCATGCCTATGATGTGACGAGCGTTACCCACTc                                                                                                                 <  5:177432/148‑1 (MQ=255)
                               aaTTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCACGCATGCCTATGATGTGACGAGCGTTACCCACTc                                                                                                                 <  3:289130/148‑1 (MQ=255)
                                       ccATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTaaa                                                                                                         <  5:306096/148‑1 (MQ=255)
                                         atatTTCTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAAccc                                                                                                      >  6:137335/1‑149 (MQ=255)
                                         atatTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACtgcat                                                                                                                                                  <  1:1536/105‑1 (MQ=255)
                                         atatTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACtgcat                                                                                                                                                  >  2:1536/1‑105 (MQ=255)
                                         atatTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCatgcatgc                                                                                                                                            <  1:295554/111‑1 (MQ=255)
                                         atatTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCatgcatgc                                                                                                                                            >  2:295554/1‑111 (MQ=255)
                                          tatTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAAccc                                                                                                      >  7:88390/1‑148 (MQ=255)
                                               cTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGaaaaa                                                                                                <  4:287301/149‑1 (MQ=255)
                                                    gagCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCa                                                                                            >  5:339541/1‑148 (MQ=255)
                                                           cagtgAAATAAGCGGCTCTCAGTTTGATTAATAGAGCTATTAATGCACGCTACCGCCACTGCCTTTACATAAACAAGAGAAATGCATGCATGCCTATGATCAGACGACCGATCCCCACACCCGCTAAACCCGAAAAATTCAAACTCTaa                                                                                    >  8:265621/3‑149 (MQ=255)
                                                            tgtgAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAAt                                                                                   >  6:208247/1‑149 (MQ=255)
                                                                 aaTAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCAAGCATGCCTATGATGTGACGAGTGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCt                                                                              <  1:85201/149‑1 (MQ=255)
                                                                   tAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTa                                                                            <  6:339541/149‑1 (MQ=255)
                                                                     aGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTAc                                                                           <  1:406659/148‑1 (MQ=255)
                                                                       cGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCaa                                                                        <  2:104167/149‑1 (MQ=255)
                                                                               aGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGctct                                                                >  7:66901/1‑149 (MQ=255)
                                                                                 tttGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTg                                                               >  3:117526/1‑148 (MQ=255)
                                                                                  ttGATTTATAGATGTATTAATTCCCGATACCGCCCCTGGCGTTACAGCTACCAGATCACTGCATGCATTCCTCTGATGTGCCGAGAGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTgg                                                              <  5:320286/148‑1 (MQ=255)
                                                                                    gATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGaaa                                                           >  2:80834/1‑149 (MQ=255)
                                                                                     aTTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAAc                                                          <  4:117526/149‑1 (MQ=255)
                                                                                      ttAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAAc                                                          >  1:425459/1‑148 (MQ=255)
                                                                                         aTAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAg                                                       <  6:153743/148‑1 (MQ=255)
                                                                                         aTAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAg                                                       <  8:88390/148‑1 (MQ=255)
                                                                                          tAGAGGTATTACTGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCTCTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGa                                                     <  8:156407/149‑1 (MQ=255)
                                                                                               gTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATc                                                <  1:80834/149‑1 (MQ=255)
                                                                                                tATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATcc                                               <  1:6549/149‑1 (MQ=255)
                                                                                                 aTTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAAtt                                                                                  >  5:145521/1‑113 (MQ=255)
                                                                                                 aTTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAAtt                                                                                  <  6:145521/113‑1 (MQ=255)
                                                                                                    aaTGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTgg                                            <  8:66901/148‑1 (MQ=255)
                                                                                                       gCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGaaaa                                        >  5:292908/1‑149 (MQ=255)
                                                                                                           gCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACttt                                    <  5:208247/149‑1 (MQ=255)
                                                                                                           gCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACttt                                    >  7:96502/1‑149 (MQ=255)
                                                                                                             tACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTa                                                                            <  1:327123/107‑1 (MQ=255)
                                                                                                             tACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTa                                                                            >  2:327123/1‑107 (MQ=255)
                                                                                                             tACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCt                                              >  4:279154/1‑137 (MQ=255)
                                                                                                             tACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCt                                              <  3:279154/137‑1 (MQ=255)
                                                                                                               ccGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGaa                                 <  7:367036/148‑1 (MQ=255)
                                                                                                                cGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACaa                                                        >  4:271693/1‑124 (MQ=255)
                                                                                                                cGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACaa                                                        <  3:271693/124‑1 (MQ=255)
                                                                                                                   cccTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATaaaa                            >  4:140118/1‑149 (MQ=255)
                                                                                                                     cTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATACAAACGCTAATTGTCTTACCAAACCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAccc                          >  4:273537/1‑149 (MQ=255)
                                                                                                                     cTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTAGGATGTGACGAGCGTTAGCCACTCGCGCTAAACCCGAAAAATTCACACGCTAATTGTCTTACCAAGCCGCGCTGGAAACAAGGAAAATCGTGGATAACTATGATTaacccc                          >  4:399737/1‑145 (MQ=255)
                                                                                                                             aCAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGc                   >  1:189390/1‑148 (MQ=255)
                                                                                                                              cAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCt                  <  5:137335/148‑1 (MQ=255)
                                                                                                                                  tACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTc             >  4:339887/1‑149 (MQ=255)
                                                                                                                                     cAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCgtt          <  7:265621/149‑1 (MQ=255)
                                                                                                                                      agagCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTATTGCTAACTCgttg         <  2:425459/149‑1 (MQ=255)
                                                                                                                                       gagCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCgttgt        >  6:131426/1‑149 (MQ=255)
                                                                                                                                             tgcatgcaTGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCt  >  2:31640/1‑149 (MQ=255)
                                                                                                                                             tgcatgcaTGCCTATGATGTGACGAGCGTTACCAACTCGCGCTAAACCCGAAAAATTCAAAAGCTAATTGTCTTACAAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCt  >  8:283561/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCT  >  NC_000913/2113033‑2113322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: