Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,801,032 C→T 100% G323E (GGA→GAA)  waaR ← lipopolysaccharide 1,2‑glucosyltransferase; UDP‑glucose:(glucosyl)LPS alpha‑1,2‑glucosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,801,0320CT100.0% 143.2 / NA 41G323E (GGA→GAA) waaRlipopolysaccharide 1,2‑glucosyltransferase; UDP‑glucose:(glucosyl)LPS alpha‑1,2‑glucosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/20);  total (21/20)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTCCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATGGATAAATTGCCCA  >  NC_000913/3800888‑3801177
                                                                                                                                                |                                                                                                                                                 
aTTTAATCCATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTga                                                                                                                                               <  6:59223/149‑1 (MQ=255)
aTTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTga                                                                                                                                               <  6:367456/149‑1 (MQ=255)
aTTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTga                                                                                                                                               <  4:95153/149‑1 (MQ=255)
aTTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTg                                                                                                                                                <  8:178684/148‑1 (MQ=255)
    aaTACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGata                                                                                                                                           >  3:397140/1‑149 (MQ=255)
    aaTACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGat                                                                                                                                            <  6:94340/148‑1 (MQ=255)
         aTTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGCTCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAat                                                                                                                                       >  6:43352/1‑148 (MQ=255)
         aTTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCATTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAatg                                                                                                                                      <  6:421144/149‑1 (MQ=255)
                                 aTCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTtata                                                                                                              <  1:217879/149‑1 (MQ=255)
                                      tAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCttt                                                                                                         >  4:44200/1‑149 (MQ=255)
                                        aaaaCAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCttttt                                                                                                       >  1:62041/1‑149 (MQ=255)
                                           acaacCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCttttttt                                                                                                     >  7:67491/1‑148 (MQ=255)
                                                  aGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCaa                                                                                             >  5:101245/1‑149 (MQ=255)
                                                        tGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAAtt                                                                                        <  8:67491/148‑1 (MQ=255)
                                                         gATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGa                                                                                      >  6:12967/1‑149 (MQ=255)
                                                               gCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTc                                                                                  <  2:355426/147‑1 (MQ=255)
                                                                 tcttctGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCa                                                                               >  8:175540/1‑148 (MQ=255)
                                                                  cttctGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCAtc                                                                             >  4:344669/1‑149 (MQ=255)
                                                                         aTCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGga                                                                      <  6:195988/149‑1 (MQ=255)
                                                                            ataataAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGagag                                                                   >  4:40658/1‑149 (MQ=255)
                                                                             taataaACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGt                                                                  >  6:390647/1‑149 (MQ=255)
                                                                                       gTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCa                                                        <  5:390647/149‑1 (MQ=255)
                                                                                       gTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCa                                                        <  7:175540/149‑1 (MQ=255)
                                                                                        ttAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGgaga                                                                    >  4:379411/1‑136 (MQ=255)
                                                                                        ttAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGgaga                                                                    <  3:379411/136‑1 (MQ=255)
                                                                                          aaaTGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCAATAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAggg                                                     >  2:296370/1‑149 (MQ=255)
                                                                                            aTGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTACAgggg                                                    >  5:1416/1‑148 (MQ=255)
                                                                                                       ggTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTT‑CAGGGGGAATTTTATTAAg                                       >  5:93333/1‑149 (MQ=255)
                                                                                                        gTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAg                                       <  1:296370/149‑1 (MQ=255)
                                                                                                           atatTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCAtctc                                                                           <  2:142295/110‑1 (MQ=255)
                                                                                                            tatTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTAt                                   >  7:165789/1‑149 (MQ=255)
                                                                                                              ttttCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTc                                                                                               >  4:45578/1‑87 (MQ=255)
                                                                                                              ttttCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTc                                                                                               <  3:45578/87‑1 (MQ=255)
                                                                                                              ttttCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTAtt                                  <  4:397140/148‑1 (MQ=255)
                                                                                                                   ggCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAg                            <  2:62041/149‑1 (MQ=255)
                                                                                                                    gCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGga                                                                      >  6:326006/1‑106 (MQ=255)
                                                                                                                     cAAAGATAACACCAACCTGCAATAATTTCTGCCATATAATGAAGTTGCACAAAAACATGGTTATAACTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTa                          <  3:44200/149‑1 (MQ=255)
                                                                                                                         gATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTAttt                       >  8:412438/1‑148 (MQ=255)
                                                                                                                               aGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGAt                <  5:12967/149‑1 (MQ=255)
                                                                                                                                      tGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATGGATaaa         >  7:190432/1‑149 (MQ=255)
                                                                                                                                             aTTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAATCTATTTTATAGTATTTTACCGATGGATAAATTGCCCa  >  3:104307/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
ATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTCCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATGGATAAATTGCCCA  >  NC_000913/3800888‑3801177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑