Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,534,954	G→T	L305L (CTC→CTA)	envZ ←	sensory histidine kinase in two‑component regulatory system with OmpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,534,954	0	G	T	100.0%	284.3 / NA	82	L305L (CTC→CTA)	envZ	sensory histidine kinase in two‑component regulatory system with OmpR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (41/41);  total (41/41)

ATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCT  >  NC_000913/3534810‑3535099                                                                                                                                                 |                                                                                                                                                  aTTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTa                                                                                                                                               <  7:351973/149‑1 (MQ=255) aTTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTa                                                                                                                                               <  1:339492/149‑1 (MQ=255)     ccATAACGGGCGGCGTTGCCCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGc                                                                                                                                           <  8:397618/149‑1 (MQ=255)      cATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCa                                                                                                                                          >  3:280395/1‑149 (MQ=255)       aTAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCAt                                                                                                                                         >  6:78212/1‑149 (MQ=255)           cGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCCCCTAGTACTGCAtt                                                                                                                                        >  1:126586/1‑146 (MQ=255)           cGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCAtt                                                                                                                                        >  5:132810/1‑146 (MQ=255)                        ccaccaTATTCGCCACCGCGCGTTTGATCGACAGCGGTTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTAATAGCCACTTTCTGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTc                                                                                                                         >  5:398174/1‑148 (MQ=255)                         caccaTATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCa                                                                                                                       >  1:10690/1‑149 (MQ=255)                         caccaTATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCa                                                                                                                       >  5:316306/1‑149 (MQ=255)                         caccaTATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCa                                                                                                                       >  3:37370/1‑149 (MQ=255)                           ccaTATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATc                                                                                                                     <  3:37846/149‑1 (MQ=255)                            caTATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCg                                                                                                                    >  8:322495/1‑149 (MQ=255)                            caTATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCg                                                                                                                    >  4:287039/1‑149 (MQ=255)                               atTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCa                                                                                                                 >  8:392750/1‑149 (MQ=255)                                ttCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCAt                                                                                                                <  5:78212/149‑1 (MQ=255)                                    ccACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCAGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTcc                                                                                                            >  6:447873/1‑149 (MQ=255)                                      acagcgcgTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTg                                                                                                          >  1:136330/4‑149 (MQ=255)                                        cgcgcgTTTGATAGCCAGCGGGTGCATTTTCACGTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGcc                                                                                                        <  4:352272/149‑1 (MQ=255)                                            cgTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCACTCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGt                                                                                                    <  5:362329/149‑1 (MQ=255)                                            cgTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGt                                                                                                    <  5:48316/149‑1 (MQ=255)                                                 gATCGACAGCTGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTAGGCAGCAATAACCTCACCTAGTACTTCATTAAGATCCTCCAATTCCATCGGCATCTCCTGCCCAGTgcg                                                                                                 >  2:395886/1‑147 (MQ=255)                                                  aTCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAg                                                                                              >  5:383113/1‑149 (MQ=255)                                                      aCAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTTGTACTGCATTAAGATCCGCCAtt                                                                                                                           <  4:160364/116‑1 (MQ=255)                                                      aCAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTGGTACTGCATTAAGATCCGCCAtt                                                                                                                           >  3:160364/1‑116 (MQ=255)                                                        aGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTc                                                                                        <  2:4098/149‑1 (MQ=255)                                                           gggTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTCCCCGGt                                                                                                    >  4:135233/1‑134 (MQ=255)                                                           gggTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTCCCCGGt                                                                                                    <  3:135233/134‑1 (MQ=255)                                                               gCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAAc                                                                                 <  1:150726/149‑1 (MQ=255)                                                                cATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACt                                                                                <  3:287039/149‑1 (MQ=255)                                                                 aTTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACATTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACt                                                                                >  6:263565/1‑148 (MQ=255)                                                                    ttCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCt                                                                             <  5:447873/148‑1 (MQ=255)                                                                    ttCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCAGCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCt                                                                             <  4:37370/148‑1 (MQ=255)                                                                       aCTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAat                                                                         <  6:446239/149‑1 (MQ=255)                                                                       aCTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAat                                                                         >  5:446239/1‑149 (MQ=255)                                                                         ttCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAatga                                                                       >  5:455508/1‑149 (MQ=255)                                                                         ttCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAatga                                                                       >  2:97200/1‑149 (MQ=255)                                                                           cAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAatgat                                                                      <  6:383113/148‑1 (MQ=255)                                                                            aaTGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAatgatg                                                                     <  2:136330/148‑1 (MQ=255)                                                                              tgctgcCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCg                                                                  >  8:374629/1‑149 (MQ=255)                                                                                  gcCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTg                                                               >  8:2148/1‑148 (MQ=255)                                                                                   ccGGGGGAAAGCGCGGTTTCAATTTCGCGCTCATAGCCACTTTCGGCTGCAATCACCTCAGCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCTGTGCGAAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCa                                                             <  6:398174/149‑1 (MQ=255)                                                                                       ggTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTaa                                                                                                                                      >  5:102662/1‑72 (MQ=255)                                                                                       ggTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTaa                                                                                                                                      <  6:102662/72‑1 (MQ=255)                                                                                        gTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGc                                                              >  7:337110/1‑143 (MQ=255)                                                                                        gTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGc                                                              <  8:337110/143‑1 (MQ=255)                                                                                         tAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTc                                                       >  7:12850/1‑149 (MQ=255)                                                                                           aaGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGa                                                     <  8:344014/149‑1 (MQ=255)                                                                                            aGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGa                                                     >  6:378313/1‑148 (MQ=255)                                                                                            aGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGa                                                     <  5:378313/148‑1 (MQ=255)                                                                                              cgcgGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGatat                                                  <  4:280395/149‑1 (MQ=255)                                                                                               gcgGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATc                                                 >  3:113616/1‑149 (MQ=255)                                                                                                cgGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCt                                                <  6:316306/149‑1 (MQ=255)                                                                                                  gTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCttt                                              <  2:12179/149‑1 (MQ=255)                                                                                                   tttCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTa                                             >  5:456143/1‑149 (MQ=255)                                                                                                    ttCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTAt                                            >  3:204914/1‑149 (MQ=255)                                                                                                       aaTTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGa                                         <  8:25281/149‑1 (MQ=255)                                                                                                       aaTTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGa                                         <  2:10690/149‑1 (MQ=255)                                                                                                       aaTTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGa                                         >  8:369899/1‑149 (MQ=255)                                                                                                       aaTTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGa                                         >  3:297198/1‑149 (MQ=255)                                                                                                        aTTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGAt                                        <  7:392750/149‑1 (MQ=255)                                                                                                        aTTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGAt                                        <  7:322495/149‑1 (MQ=255)                                                                                                             ccGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCt                                                                             >  6:171831/1‑107 (MQ=255)                                                                                                             ccGCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCt                                                                             <  5:171831/107‑1 (MQ=255)                                                                                                               gCTCATAGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCt                                 <  1:421261/149‑1 (MQ=255)                                                                                                                     aGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGc                               >  4:94218/1‑145 (MQ=255)                                                                                                                     aGCCACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGc                               <  3:94218/145‑1 (MQ=255)                                                                                                                       ccACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCaa                                                                          >  8:254581/1‑100 (MQ=255)                                                                                                                       ccACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGa                                         >  4:442918/1‑133 (MQ=255)                                                                                                                       ccACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGa                                         <  3:442918/133‑1 (MQ=255)                                                                                                                       ccACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATa                         <  1:395886/149‑1 (MQ=255)                                                                                                                       ccACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATa                         <  7:369899/149‑1 (MQ=255)                                                                                                                        cACTTTCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAg                        >  6:358557/1‑149 (MQ=255)                                                                                                                           tttCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCa                     >  5:262484/1‑149 (MQ=255)                                                                                                                             tCGGCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCa                     <  6:455508/147‑1 (MQ=255)                                                                                                                               ggCAGCAATCACCTCACCTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCt                 <  5:263565/149‑1 (MQ=255)                                                                                                                                         acctcaccTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGAt                                        >  4:415336/1‑116 (MQ=255)                                                                                                                                         acctcaccTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGAt                                        <  3:415336/116‑1 (MQ=255)                                                                                                                                           ctcaccTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCtcatca     >  4:213513/1‑149 (MQ=255)                                                                                                                                              accTAGTACTGCATTCAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTCCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCatcatc   <  6:132810/148‑1 (MQ=255)                                                                                                                                              accTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCAtct  <  7:385484/149‑1 (MQ=255)                                                                                                                                              accTAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCAtct  >  8:385484/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                  ATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCAATGCTGCCGGGGTAAAGCGCGGTTTCAATTTCCCGCTCATAGCCACTTTCGGCAGCAATCACCTCACCGAGTACTGCATTAAGATCCGCCATTTCCATCGGCATCTCCTGCCCGGTGCGCAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCT  >  NC_000913/3534810‑3535099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑